Effect of N-stearoylethanolamine on free amino acid levels in rat plasma and liver with burn

The effect of the endocannabinoid congener N-stearoylethanolamine (NSE) on the content of plasma and liver free amino acids in burned rats was studied. The animals after the thermal burn of the skin received per os during 7 days the water suspension of NSE in the doze 10 mg/kg of body weight. In the other group of rats the suspension was applied on the wound (the concentration of NSE was 10 mg/ml). In experimental animals the total amount of free amino acids in plasma decreased after burn while the total amount of free amino acids in liver significantly increased. In burn animals the ratio of plasma and liver Phe/Tyr and Gly/Val was found to be augmented while the Fischer ratio (Ile+Leu+Val/Phe+Tyr) diminished compared with intact rats. It was shown for the first time that NSE caused normalization of the concentration of some free amino acids and of Phe/Tyr, Gly/Val ratios, and the Fischer ratio in blood plasma and liver of rats with the burn injury.Altogether, these data demonstrate that NSE possesses adaptogenic properties, and is involved in the organism response to a burn. These findings suggest possibility of NSE usage for burn treatment.

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EFFECT OF CORTISOL TREATMENT ON FREE AMINO ACID LEVELS IN RATS

Journal of Endocrinology ◽

10.1677/joe.0.0590057 ◽

1973 ◽

Vol 59 (1) ◽

pp. 57-63 ◽

Cited By ~ 8

Author(s):

W. DE LOECKER ◽

M. L. STAS

Keyword(s):

Skeletal Muscle ◽

Amino Acids ◽

Amino Acid ◽

Free Amino Acid ◽

Protein Turnover ◽

Free Amino Acids ◽

Free Amino ◽

Experimental Period ◽

Amino Acid Levels ◽

Amino Acid Concentrations

SUMMARY Changes in the concentrations of free amino acids in intracellular fluids and blood plasma were measured in rats treated with cortisol. Increasing age raised the concentrations of free amino acids in plasma, while in liver, with the exception of glycine and alanine, decreased concentrations were observed. Cortisol treatment reduced free amino acid levels in plasma and liver which suggested a progressive catabolism of body proteins and increased protein synthesis in the liver. In skeletal muscle of control rats the free amino acid concentrations increased during the experimental period. Cortisol increased the concentration of certain amino acids and decreased that of others due to an increased protein turnover in muscle.

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Free Amino Acids as Regulators of Osmotic Pressure in Aquatic Insect Larvae

Journal of Experimental Biology ◽

10.1242/jeb.101.1.153 ◽

1982 ◽

Vol 101 (1) ◽

pp. 153-160 ◽

Cited By ~ 7

Author(s):

H.A. EDWARDS

Keyword(s):

Amino Acids ◽

Osmotic Pressure ◽

Free Amino Acids ◽

Free Amino ◽

Organic Molecules ◽

Aquatic Insect ◽

Insect Larvae ◽

Inorganic Ion ◽

Amino Acid Levels ◽

Aquatic Insect Larvae

Microanalytical techniques are used to show that organic molecules play an important role is osmoregulation. Changes in the inorganic ion composition of haemolymph from fourth instar larvae of Aedes aegypti are correlated with the changes in the concentration of organic ions. Free amino acids have a significant role in regulating haemolymph osmotic pressure with respect to the osmotic pressure of the water in which the animal lives. Mechanisms by which amino acid levels could respond to changes in salt concentration are discussed.

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A COMPARISON OF THE EFFECT OF DIETARY FAT AND CARBOHYDRATE ON FREE AMINO ACIDS IN THEBLOOD PLASMA OF CHICKS

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y67-025 ◽

1967 ◽

Vol 45 (2) ◽

pp. 225-234 ◽

Cited By ~ 8

Author(s):

Tsang-Cheng Shao ◽

D. C. Hill

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Dietary Fat ◽

Free Amino Acids ◽

Free Amino ◽

Plasma Amino Acid ◽

Considerable Time ◽

Lower Plasma ◽

Amino Acid Levels ◽

Plasma Free Amino Acids

As previously reported, an increased concentration of plasma free amino acids, particularly lysine and threonine, was found when chicks were fasted for 24 hours. This increase was suppressed where a nonprotein diet was fed. Dextrose as the chief energy source in the nonprotein diet was more effective than shortening or lard in this respect. However, the degree to which the two energy sources differed was less where birds were adapted to the utilization of fat by feeding a diet rich in fat for a considerable time before making the comparison. Insulin administration to fasted chicks was found to lower plasma amino acid levels. The possible influence of interrelationships among dextrose, fat, and insulin on plasma amino acid levels is discussed.

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Use of NMR spectroscopy in the analysis of carnosine and free amino acids in fermented sausages during ripening

Chemical Papers ◽

10.1515/chempap-2015-0148 ◽

2015 ◽

Vol 69 (10) ◽

Cited By ~ 4

Author(s):

Marcel Mati ◽

Ladislav Staruch ◽

Michal Šoral

Keyword(s):

Amino Acids ◽

Nmr Spectroscopy ◽

Free Amino Acids ◽

Free Amino ◽

High Fat ◽

Nmr Method ◽

Fermented Sausages ◽

Quantitative Changes ◽

First Time

AbstractQuantitative changes of carnosine and free amino acids in high-fat (43-50 mass %) fermented sausages during ripening were analysed using a 600 MHz VNMRS NMR spectrometer. Seven free amino acids were identified in the samples and a relatively high content of carnosine was observed in the final stage of ripening. The NMR method for the determination of free amino acids and carnosine content applied in this work has been used for the first time and it has proven to be suitable for the analysis of fermented sausages.

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Metabolic profile analysis of free amino acids in experimental autoimmune uveoretinitis rat plasma

International Journal of Ophthalmology ◽

10.18240/ijo.2019.01.03 ◽

2019 ◽

Cited By ~ 1

Keyword(s):

Amino Acids ◽

Free Amino Acids ◽

Free Amino ◽

Metabolic Profile ◽

Profile Analysis ◽

Rat Plasma ◽

Experimental Autoimmune Uveoretinitis ◽

Experimental Autoimmune

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The variation in amino acid levels in the blood plasma of breeding roosters during sexual maturation*

Archives Animal Breeding ◽

10.5194/aab-45-279-2002 ◽

2002 ◽

Vol 45 (3) ◽

pp. 279-285

Author(s):

E. Straková ◽

P. Suchý ◽

V. Večerek ◽

L. Máchal

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Blood Plasma ◽

Free Amino Acid ◽

Sexual Maturation ◽

Free Amino Acids ◽

Free Amino ◽

Molar Concentration ◽

Dynamic Changes ◽

Amino Acid Levels

Abstract. The paper provides an overview of results obtained in the study of variation in free amino acid levels in the blood plasma of breeding roosters (RIR 05 line) during sexual maturation (in the 10th, 15th, 20th and 25th weeks of age). The results prove that dynamic changes in free amino acid concentrations in blood plasma are connected with the sexual maturation of breeding roosters. During this period the concentrations of each particular free amino acid varied at a significance level of P ≤ 0.05 and P ≤ 0.01. Furthermore, in most cases the plasma concentrations of free amino acids in the 10th and 15th week were higher in comparison with those in the 20th and 25th week. Interestingly, the concentrations of individual free amino acids ranged over a wide interval (units, tens and hundreds of μmol/l). In this respect the free amino acids in plasma can be divided into three groups. The first group consisted of amino acids that occurred at low molar concentration (up to 100 μmol/l): cysteic acid, aspartic acid, α-aminoadipic acid, methionine, isoleucine, γ-aminobutyric acid, 3-methylhistidine, tryptophan and ornitine. The second group included amino acids occurring at medium to high molar concentration (100–300 μmol/l): taurine, glutamine, 1/2 cystine, valine, leucine, tyrosine, phenylalanine, histidine, lysine and arginine. Finally, the third group contained threonine, serine, glutamic acid, proline, glycine and alanine that were present at high molar concentrations (above 300 μmol/l). Analyses of plasma in the period from the 10th to 25th week detected dynamic changes in levels of individual free amino acids and showed that the total content of these amino acids gradually decreased to the following mean values: 5 685.00 μmol/l in the 10th week, 5 076.21 μmol/l in the 15th week, 4 384.78 μmol/l in the 20th week and 4 793.30 μmol/l in the 25th week.

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