scholarly journals Is rapid diagnostic test (malaria Pv/Pf Ag card test) reliable in diagnosing malaria

2017 ◽  
Vol 5 (1) ◽  
pp. 92
Author(s):  
Vishruti Gandhi Vishruti Gandhi ◽  
Prasad Muley ◽  
Niyati Parikh ◽  
Hardik Gandhi ◽  
Akash Mehta
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Vivax ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Sensitivity And Specificity ◽  
Peripheral Blood Smear ◽  
Peripheral Smear ◽  
Infected Female ◽  
Anopheles Mosquitoes ◽  
Smear Examination

Background: Malaria is a protozoan disease transmitted by the bite of infected female anopheles mosquitoes is one of the most important parasitic diseases of human with transmission in 109 countries, affecting more than one billion people worldwide. This study was planned to compare the gold standard i.e. peripheral blood smear examination and the newer rapid diagnostic test (malaria plasmodium falciparum/ plasmodium vivax antigen card) to know the diagnostic accuracy of Rapid Diagnostic Test (RDT) kits. Methods: All the suspected cases of WHO defined malaria between 1month to 18 years of age were enrolled in the study.Results: Out of 96 clinically suspected cases of malaria 63 were confirmed by peripheral smear. The age range of participants ranged from 4 months to 17 years. On peripheral smear examination, out of 96 clinically suspected cases, 37 (38.5%) cases were positive for P. vivax, 23 (23.9%) were positive for P. falciparum and 3 (3.1%) were positive for both parasites by microscopy. Sensitivity and specificity of RDT for Plasmodium Vivax is 92.5% and 96.4% respectively. Sensitivity and specificity of RDT for Plasmodium Falciparum is 96.2% and 90%.Conclusions: The rational use of RDTs as a complement to microscopy might give substantial health benefits through earlier treatment, reduction in morbidity and mortality and more rationalized approach for choosing anti-malarial drugs, which in terms may prevent drug resistance.

scholarly journals Improving the Specificity of Plasmodium falciparum Malaria Diagnosis in High-Transmission Settings with a Two-Step Rapid Diagnostic Test and Microscopy Algorithm

2017 ◽  
Vol 55 (5) ◽  
pp. 1540-1549 ◽  
Author(s):  
Moses Murungi ◽  
Travis Fulton ◽  
Raquel Reyes ◽  
Michael Matte ◽  
Moses Ntaro ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Real Time ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Sensitivity And Specificity ◽  
Real Time Pcr ◽  
High Transmission ◽  
Transmission Setting ◽  
Multiple Antigen ◽  
Step Algorithm

ABSTRACT Poor specificity may negatively impact rapid diagnostic test (RDT)-based diagnostic strategies for malaria. We performed real-time PCR on a subset of subjects who had undergone diagnostic testing with a multiple-antigen (histidine-rich protein 2 and pan -lactate dehydrogenase pLDH [HRP2/pLDH]) RDT and microscopy. We determined the sensitivity and specificity of the RDT in comparison to results of PCR for the detection of Plasmodium falciparum malaria. We developed and evaluated a two-step algorithm utilizing the multiple-antigen RDT to screen patients, followed by confirmatory microscopy for those individuals with HRP2-positive (HRP2 + )/pLDH-negative (pLDH − ) results. In total, dried blood spots (DBS) were collected from 276 individuals. There were 124 (44.9%) individuals with an HRP2 + /pLDH + result, 94 (34.1%) with an HRP2 + /pLDH − result, and 58 (21%) with a negative RDT result. The sensitivity and specificity of the RDT compared to results with real-time PCR were 99.4% (95% confidence interval [CI], 95.9 to 100.0%) and 46.7% (95% CI, 37.7 to 55.9%), respectively. Of the 94 HRP2 + /pLDH − results, only 32 (34.0%) and 35 (37.2%) were positive by microscopy and PCR, respectively. The sensitivity and specificity of the two-step algorithm compared to results with real-time PCR were 95.5% (95% CI, 90.5 to 98.0%) and 91.0% (95% CI, 84.1 to 95.2), respectively. HRP2 antigen bands demonstrated poor specificity for the diagnosis of malaria compared to that of real-time PCR in a high-transmission setting. The most likely explanation for this finding is the persistence of HRP2 antigenemia following treatment of an acute infection. The two-step diagnostic algorithm utilizing microscopy as a confirmatory test for indeterminate HRP2 + /pLDH − results showed significantly improved specificity with little loss of sensitivity in a high-transmission setting.

scholarly journals The sensitivity and specificity of a urine based Rapid Diagnostic Test for the diagnosis of plasmodium falciparum in a malaria endemic area in Odisha, India

2017 ◽  
Vol 111 (7) ◽  
pp. 383-387 ◽  
Author(s):  
Ajit Gopal Samal ◽  
Prativa Kumari Behera ◽  
Akshay Kumar Mohanty ◽  
Sanghamitra Satpathi ◽  
Abhishek Kumar ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Sensitivity And Specificity ◽  
Endemic Area ◽  
Malaria Endemic Area

Rapid diagnostic test for G6PD deficiency in Plasmodium vivax -infected men: a budget impact analysis based in Brazilian Amazon

2016 ◽  
Vol 22 (1) ◽  
pp. 21-31
Author(s):  
Henry Maia Peixoto ◽  
Marcelo Augusto Mota Brito ◽  
Gustavo Adolfo Sierra Romero ◽  
Wuelton Marcelo Monteiro ◽  
Marcus Vinícius Guimarães de Lacerda ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
G6pd Deficiency ◽  
Impact Analysis ◽  
Budget Impact ◽  
Brazilian Amazon ◽  
Budget Impact Analysis

COMPARISON OF FOURTH GENERATION ELISA AND RAPID DIAGNOSTIC TEST FOR DIAGNOSIS OF HEPATITIS C VIRUS(HCV) INFECTION IN A TERTIARY CARE HOSPITAL.

2021 ◽  
pp. 15-17
Author(s):  
Stuti Kansra Arora ◽  
Mala Chhabra ◽  
Anuradha Anuradha ◽  
Arvind Achra ◽  
Nandini Duggal
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Sensitivity And Specificity ◽  
Treatment Initiation ◽  
Tertiary Care ◽  
Hcv Infection ◽  
Fourth Generation ◽  
World Population ◽  
Test Results ◽  
Blood Samples

Introduction:Hepatitis C virus (HCV) infection has emerged as one of the major global health challenge affecting about 2 - 3% of the world population. Epidemiological studies have shown that HCV infection is a major risk factor for development of Acute hepatitis,chronic liver disease,cirrhosis and Hepatocellular carcinoma (HCC).Early diagnosis of HCV is important to link hepatitis testing to care and treatment initiation. Aim:To compare sensitivity and specificity of rapid diagnostic test (RDT) with fourth generation ELISA Material and Method: This study was conducted in the Department of Microbiology at Atal Bihari Vajpayee Institute of Medical Sciences (formerly Post Graduate Institute of Medical Education and Research) and Dr Ram Manohar Lohia Hospital from January 2018 to December 2018.Blood samples of patients suspected with hepatitis were tested using ELISA and rapid diagnostic test Results: In our study 26378 blood samples were tested for HCV,using fourth generation ELISA.Of these,581(2.20%) samples were found to be positive by ELISA.These HCV positive samples along with equal number of ELISA negative samples were tested by rapid diagnostic test. Sensitivity and specificity of the rapid diagnostic test was found to be 72.98 % and 100% respectively. Discussion:Rapid diagnostic test can be used during emergency hours but their results must be followed by ELISA test results in a tertiary care hospital.Reporting of false negative results should be minimized for rapid linkage to treatment initiation and to avoid silent transmission of infection.

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