scholarly journals Slit skin smear or Fite-Faraco staining of tissue sample, which is a better indicator of bacillary load?

2021 ◽  
Vol 7 (5) ◽  
pp. 684
Author(s):  
Shruti Appannavar ◽  
Kiran . ◽  
Amit Ashok Ingalagi ◽  
Rohith .
Keyword(s):  
Tissue Sample ◽  
Cutaneous Nerve ◽  
Reliable Indicator ◽  
Acid Fast Bacilli

<p><strong>Background: </strong>Leprosy is clinically diagnosed on the basis of presence of following cardinal signs: 1. Hypopigmented or erythematous anesthetic patch on skin, 2. Thickened and/or tender peripheral/cutaneous nerve, 3. Acid fast bacilli in slit skin smear. This study aims at comparing bacillary indices in slit skin smears with that from biopsy samples.</p><p><strong>Methods: </strong>After obtaining informed written consent, slit skin smears were performed and observed for acid fast bacilli. Punch biopsies of lesions were taken and processed and stained with Fite-Faraco technique. The presence or absence of acid-fast bacilli in both modalities was noted.</p><p><strong>Results: </strong>Slit skin smears were positive in 24 patients and negative in the rest. And 26 patient’s biopsy reports had Fite-Faraco positivity, out of the total 46 patients. The p=0.67 which was not significant at significance value 0.05.</p><p><strong>Conclusions: </strong>Skin smear is an equally reliable indicator of bacillary load as Fite-Faraco bacillary index in tissue.</p><p><strong> </strong></p>

Biomedical applications: Pitfalls in the practice

1994 ◽  
Vol 52 ◽  
pp. 378-379
Author(s):  
J. D. Shelburne ◽  
Peter Ingram ◽  
Victor L. Roggli ◽  
Ann LeFurgey
Keyword(s):  
Operating Room ◽  
Liquid Nitrogen ◽  
Lung Tissue ◽  
Biomedical Applications ◽  
Microprobe Analysis ◽  
Tissue Sample ◽  
Cellular Level ◽  
Tissue Samples ◽  
Asbestos Fibers

At present most medical microprobe analysis is conducted on insoluble particulates such as asbestos fibers in lung tissue. Cryotechniques are not necessary for this type of specimen. Insoluble particulates can be processed conventionally. Nevertheless, it is important to emphasize that conventional processing is unacceptable for specimens in which electrolyte distributions in tissues are sought. It is necessary to flash-freeze in order to preserve the integrity of electrolyte distributions at the subcellular and cellular level. Ideally, biopsies should be flash-frozen in the operating room rather than being frozen several minutes later in a histology laboratory. Electrolytes will move during such a long delay. While flammable cryogens such as propane obviously cannot be used in an operating room, liquid nitrogen-cooled slam-freezing devices or guns may be permitted, and are the best way to achieve an artifact-free, accurate tissue sample which truly reflects the in vivo state. Unfortunately, the importance of cryofixation is often not understood. Investigators bring tissue samples fixed in glutaraldehyde to a microprobe laboratory with a request for microprobe analysis for electrolytes.

Classification of small type B/C follicles as primordial follicles in mature rats

Reproduction ◽  
2000 ◽  
pp. 43-48 ◽  
Author(s):  
S Meredith ◽  
G Dudenhoeffer ◽  
K Jackson
Keyword(s):  
Dna Synthesis ◽  
Granulosa Cells ◽  
Single Layer ◽  
Reliable Indicator ◽  
Type B ◽  
Primordial Follicles ◽  
Type C

In the present study, follicles were classified according to the morphology of their granulosa cells. Type B follicles contained only flattened granulosa cells; type B/C follicles had a mixture of flattened and cuboidal granulosa cells in a single layer, and type C follicles had a single layer of cuboidal granulosa cells. The primary objectives of the study were to determine whether 5-bromo-2-deoxyuridine incorporation into type B/C follicles was a marker for initiation of growth and how long type B/C follicles could remain at the same stage before transformation to type C follicles. Female Holtzman rats received bromo-deoxyuridine for 7 days. After the infusion (day minipumps were removed = day 0), rats were ovariectomized on days 0 (n = 9), 30 (n = 8), 90 (n = 8) and 150 (n = 9). The numbers of type B, B/C and C follicles within one ovary were determined using modified fractionator counting. Analysis over all times demonstrated that there were more (P < 0.0001) type B/C (941 +/- 61 per ovary) than type C (140 +/- 18 per ovary) or type B (159 +/- 19 per ovary) follicles. The numbers of type B and type C follicles did not differ from each other at any time. Only one of 34 rats evaluated had bromo-deoxyuridine-labelled type B follicles. On day 150, 57% of the bromo-deoxyuridine-labelled type B/C follicles remained from day 0. It is concluded that (1) DNA synthesis in granulosa cells of type B/C follicles is not a reliable indicator of impending growth; and (2) type B and type B/C follicles are both components of the pool of primordial follicles.

Harvesting Split-thickness Skin Grafts with Lateral Femoral Cutaneous Nerve Block

2004 ◽  
Vol 66 (6) ◽  
pp. 612-614
Author(s):  
Yukiko TERAMOTO ◽  
Makoto ICHIMIYA ◽  
Yuko TAKITA ◽  
Yoshiaki YOSHIKAWA ◽  
Masahiko MUTO
Keyword(s):  
Nerve Block ◽  
Cutaneous Nerve ◽  
Lateral Femoral Cutaneous Nerve ◽  
Skin Grafts ◽  
Thickness Skin ◽  
Femoral Cutaneous Nerve
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