Protective effects of riboflavin-UVA-mediated posterior sclera collagen cross-linking in a guinea pig model of form-deprived myopia

AIM: To evaluate the effect of posterior sclera collagen cross-linking induced by riboflavin-ultraviolet A (UVA) on form-deprived myopia in guinea pigs. RESULTS: After 4wk, the interocular difference of refractive error were -0.11±0.67, -2.93±0.56, 1.10±0.58, and -1.63±0.41 D in the NOR, FDM, NOR+CL, and FDM+CL groups respectively. Mixed-effect linear model revealed significant effect of FDM (P<0.01) and CL (P<0.001). Also, after 4wk, the interocular difference of axial length were 0.01±0.04, 0.29±0.07, -0.13±0.06, and 0.11±0.05 mm in the NOR, FDM, NOR+CL, and FDM+CL group. Mixed-effect linear model revealed significant effect of FDM (P<0.001) and CL (P<0.01). As for corneal curvature, significant interocular difference have not found between any of the two groups. At the end of this experiment, the ultimate stress and elastic modulus were found significantly increased in both CL groups. But no difference was found in the groups without cross-linked. There was no abnormality observed in the retina and RPE cells of the treated eyes. CONCLUSION: The posterior sclera collagen cross-linking induced by riboflavin-UVA can slow down the progress of myopia and increase the sclera biomechanical strength in the guinea pig model of form-deprived myopia.

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Protective Effects of SM-20302, an Orally Active GPIIb/IIIa Antagonist, in an ADP/Epinephrine-Induced Guinea Pig Model of Transient Cerebral Ischemia

Thrombosis Research ◽

10.1016/s0049-3848(00)00378-9 ◽

2001 ◽

Vol 101 (3) ◽

pp. 119-126 ◽

Cited By ~ 4

Author(s):

Seiya Horisawa ◽

Munekiyo Kaneko ◽

Teruo Sakurama

Keyword(s):

Cerebral Ischemia ◽

Guinea Pig ◽

Transient Cerebral Ischemia ◽

Pig Model ◽

Protective Effects ◽

Guinea Pig Model ◽

Orally Active

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Chloroquine inhibited Ebola virus replication in vitro but failed to protect against infection and disease in the in vivo guinea pig model

Journal of General Virology ◽

10.1099/jgv.0.000309 ◽

2015 ◽

Vol 96 (12) ◽

pp. 3484-3492 ◽

Cited By ~ 59

Author(s):

Stuart D. Dowall ◽

Andrew Bosworth ◽

Robert Watson ◽

Kevin Bewley ◽

Irene Taylor ◽

...

Keyword(s):

Guinea Pig ◽

Ebola Virus ◽

Pig Model ◽

Human Populations ◽

Therapeutic Effects ◽

Protective Effects ◽

Highly Pathogenic ◽

Guinea Pig Model

Ebola virus (EBOV) is highly pathogenic, with a predisposition to cause outbreaks in human populations accompanied by significant mortality. Owing to the lack of approved therapies, screening programmes of potentially efficacious drugs have been undertaken. One of these studies has demonstrated the possible utility of chloroquine against EBOV using pseudotyped assays. In mouse models of EBOV disease there are conflicting reports of the therapeutic effects of chloroquine. There are currently no reports of its efficacy using the larger and more stringent guinea pig model of infection. In this study we have shown that replication of live EBOV is impaired by chloroquine in vitro. However, no protective effects were observed in vivo when EBOV-infected guinea pigs were treated with chloroquine. These results advocate that chloroquine should not be considered as a treatment strategy for EBOV.

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