scholarly journals Efficacy of Cymbopogon citratus Stapf leaf extract as seed protectant against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) on stored maize (Zea mays L.)

2017 ◽  
Vol 30 (4) ◽  
pp. 220
Author(s):  
Diligent Oboho ◽  
Joseph Eyo ◽  
Felicia Ekeh ◽  
Solomon Okweche

A laboratory experiment was conducted to evaluate the insecticidal property of <em>Cymbopogon citratus</em> leaf extract as seed protectant against maize weevil. One (1) gram each of acetone, chloroform, methanol and aqueous extracts were re-suspended in 5 ml of deionized water and used to impregnate filter papers set in four replicates. Varied concentrations of the aqueous extract per 50 g of <em>Zea mays</em> grains infested with 10 pairs of sexed <em>Sitophilus zeamais</em> for 28 days were tested for insecticidal properties and compared with experimental and synthetic conventional insecticide [Coopex (0.25 g)] controls. Parameters assessed were effect of <em>C. citratus</em> extracts on weevil mortality (toxicity test) and protection of maize against <em>S. zeamais</em>. Data obtained were analyzed using Analysis of Variance (ANOVA) and means were separated using New Duncan Multiple Range Test at 5% level of significance. Results showed significant (P &lt; 0.05) concentration and duration dependent mortalities of <em>S. zeamais</em>. The aqueous extract gave the highest protection of the maize grains followed by chloroform, methanol and acetone extracts respectively. The insecticidal potency of <em>C. citratus</em> extracts and its availability places it as an attractive biopesticide in traditional post-harvest seed protection.

2017 ◽  
Vol 30 (4) ◽  
pp. 220
Author(s):  
Diligent Oboho ◽  
Joseph Eyo ◽  
Felicia Ekeh ◽  
Solomon Okweche

A laboratory experiment was conducted to evaluate the insecticidal property of <em>Cymbopogon citratus</em> leaf extract as seed protectant against maize weevil. One (1) gram each of acetone, chloroform, methanol and aqueous extracts were re-suspended in 5 ml of deionized water and used to impregnate filter papers set in four replicates. Varied concentrations of the aqueous extract per 50 g of <em>Zea mays</em> grains infested with 10 pairs of sexed <em>Sitophilus zeamais</em> for 28 days were tested for insecticidal properties and compared with experimental and synthetic conventional insecticide [Coopex (0.25 g)] controls. Parameters assessed were effect of <em>C. citratus</em> extracts on weevil mortality (toxicity test) and protection of maize against <em>S. zeamais</em>. Data obtained were analyzed using Analysis of Variance (ANOVA) and means were separated using New Duncan Multiple Range Test at 5% level of significance. Results showed significant (P &lt; 0.05) concentration and duration dependent mortalities of <em>S. zeamais</em>. The aqueous extract gave the highest protection of the maize grains followed by chloroform, methanol and acetone extracts respectively. The insecticidal potency of <em>C. citratus</em> extracts and its availability places it as an attractive biopesticide in traditional post-harvest seed protection.


2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Alfia Wulansari ◽  
Nur Rochman ◽  
Setyono Setyono

Sitophilus zeamais Motsch. is a primary storage insect pest damaging maize grains. Control of S. zeamais can be done by applying biopesticide. This study was aimed at assessing the ability of Jeruk Purut leaf extract, as biopesticide, to kill and repel Sitophilus zeamais Motsch. The study was conducted at Entomology Laboratory, Seameo Biotrop in March to August 2018. A completely randomized design with three replicates in each rate of Jeruk Purut leaf extract was used. In preliminary pesticide toxicity experiment, five rates of Jeruk Purut leaf extract, namely 0%, 2%, 4%, 6%, and 8% were applied. In preliminary repelling ability experiment, five rates of Jeruk Purut leaf extract, namely 1%, 2%, 4%, 6%, and 8% were applied. In the main pesticide toxicity and repelling ability trial, five rates of Jeruk Purut leaf extract, namely 3%, 6%, 9%, 12%, and 15% were applied. Results showed that in 24 hour after treating (HAT) no significant effects were found. Significant effects of Jeruk Purut leaf extract as insecticide on S. zeamais were found in 48 and 72 HAT. The highest mortality rate (82.2%) of S. zeamais was found in 15% rate of Jeruk Purut leaf extract in 72 HAT. The application of Jeruk Purut leaf extract was not found to give significant effects on repelling S. zeamais in 24, 48, and 72 HAT as indicated in their sharply fluctuating repellent values. The highest repelling ability (95%) was found in the application of 9% Jeruk Purut leaf extract in 24 HAT. It was concluded that Jeruk Purut leaf extract was more potential as insecticide repellent for Sitophilus zeamais Motsch. Key words: biopesticide, repellent, insecticide, maize, primary storage insect pest


2021 ◽  
Vol 11 (1) ◽  
pp. 34-44
Author(s):  
Susmita Gautam ◽  
Subodh Khanal ◽  
Dipak Khanal ◽  
Saroj Raj Mishra ◽  
Sunil Ghimire

Laboratory experiment was done to perform the phytochemical screening of six locally available botanicals (Azadirachta indica Juss, Artemisia dubia Wall, Lantana camara L, Parthenium hysterophorus L, Justicia adhatoda Linn. and Moringa oleifera L.) and their effectiveness to control the maize weevil (Sitophilus zeamais Motsch.) at Agriecology lab of Institute of Agriculture and Animal Science, Paklihawa Campus, Rupandehi, Nepal. Seven treatments (leaf extract of A. indica, A. dubia, L. camara, P. hysterophorus. J. adhatoda and M. oleifera and distilled water as control) applied at 3 different doses (0.5, 1 & 2g/50g seeds) were arranged in CRD design with 3 replications. Result of mortality test of maize weevil revealed that, at highest concentration of L. camara powder (T17- 2g/50 gm seeds) mortality of maize weevil was observed up to 85% at 45 DAT. Whereas number of damaged grain was lowest in T17 (L. camara powder- 2g/50 gm seeds). The effectiveness of botanicals was found to increase with increasing the concentration. Besides that, Justicia adhatoda (2g/50g seeds) and Artemisia dubia (1g/50g seeds) were also found as good candidates to use for the control of Sitophilus zeamais. Qualitative tests done for the presence of various phytochemicals showed that Azadirachta indica Juss and Artemisia dubia Wall. were found to contain most of the tested metabolites among all botanicals. Most of those phytochemicals were extracted by the aqueous solution rather than other 3 menstrums used (acetone, methanol and petroleum ether). Similarly, quantitative test revealed that percentage of alkaloid was found 18.01%, 17.78%, 17.75%, 11.58%, 8.20%, 6.78% and flavonoid15.32%, 13.63%, 12.86%, 11.36%, 7. 51% 2.85% respectively in L. camara L. P. hysterophorus L., J. adhatoda Linn., M. oleifera L., A. indica Juss. and A. dubia Wall. Leaf extract of L. camera found to contain highest botanicals extract reach in alkaloid and flavonoid mayhave insecticidal property and could be use as grain protectant percentage of alkaloid (18.01%) as well as flavonoid (15.32%) So, it was concluded that the locally available against the maize weevil at storage condition in cost effective and eco-friendly manner; however, application at proper dose is necessary to maintain the efficacy at higher level.


2012 ◽  
Vol 38 (11) ◽  
pp. 1402-1409 ◽  
Author(s):  
Donald A. Ukeh ◽  
Christine M. Woodcock ◽  
John A. Pickett ◽  
Michael A. Birkett

2017 ◽  
Vol 52 (4) ◽  
pp. 337-342
Author(s):  
SA Babarinde ◽  
MO Petinrin ◽  
AD Adeleye ◽  
BS Ajani

Toxicity of Sclerocarya birrea A. Rich. (Anacardiaceae) leaf and bark and Azadirachta indica A. Juss (Meliaceae) leaf extracts against Sitophilus zeamais Motchulsky (Coleoptera: Curculionidae) was evaluated under the laboratory conditions (77±4% relative humidity and 27±3°C temperature). When extracted doses were compared, mortality was dose- and exposure period-dependent. At 1 DAT, A. indica leaf extract had significantly (p<0.05) higher mortality (12.50-15.00%) than that of S. birrea bark extract (5.00-15.50%). However at 5 DAT, when 0.45 ml/33 cm2 was applied, mortality was not significantly (p>0.05) different and ranged from 91.25 to 97.5% for all the botanicals. When the extracting solvents were compared, mortality was exposure period-dependent with highest percentage mortality (99.17%) recorded at 5 DAT in S. birrea petroleum ether leaf extract, which was significantly higher than that of mortality observed in filter paper treated with the petroleum ether extracts of other botanicals. However, there was no significant difference in the effect of the two studied solvents. The results highlight the insecticidal potentials of the selected Nigerian-grown botanicals against maize weevil and recommend them as component of weevil Integrated Pest Management, in the areas where the botanicals are abundant.Bangladesh J. Sci. Ind. Res. 52(4), 337-342, 2017


2013 ◽  
Vol 54 ◽  
pp. 26-33 ◽  
Author(s):  
Thiago Henrique Napoleão ◽  
Bernardo do Rego Belmonte ◽  
Emmanuel Viana Pontual ◽  
Lidiane Pereira de Albuquerque ◽  
Roberto Araújo Sá ◽  
...  

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