scholarly journals Enzymatic profile, adhesive and invasive properties of Candida albicans under the influence of selected plant essential oils.

2014 ◽  
Vol 61 (1) ◽  
Author(s):  
Aleksandra Budzyńska ◽  
Beata Sadowska ◽  
Marzena Więckowska-Szakiel ◽  
Barbara Różalska
Keyword(s):  
Candida Albicans ◽  
Essential Oils ◽  
Virulence Factors ◽  
Significant Activity ◽  
Lemon Balm ◽  
Minimal Inhibitory Concentrations ◽  
Plant Essential Oils ◽  
Significant Difference ◽  
Germ Tubes ◽  
Fibroblast Monolayer

The influence of essential oils (EOs) used at sublethal level, on the presence and intensity of Candida albicans virulence factors was evaluated. Minimal inhibitory concentrations (MICs) of Lemon balm, Citronella, Geranium and Clove oils were established as 0.097% (v/v). Using the agar plates with substrates for proteases, phospholipases and hemolysins it was shown that C. albicans ATCC 10231 and C. albicans ATCC 90028 strains differed in the type and amount of enzymes produced. No significant difference in their total amount could be detected after pretreatment for 24 h with EOs at ½ MIC. However, the short-term (1 h) acting oils at MIC caused a statistically significant reduction in this activity. In the API ZYM test it was demonstrated that both strains exhibited activity of the same 9 out of 19 enzyme types and that EOs caused a significant decrease in the release of some of them. In the presence of subMIC of EOs, or when the fungus had previously been exposed to the MIC of oil, germ tubes formation was significantly and irreversibly reduced. Such C. albicans spotted on the Spider agar containing EOs at subMICs were unable to penetrate the agar. A significant decrease in the C. albicans adhesion to the fibroblast monolayer with respect to controls was also demonstrated when yeasts had been exposed to EOs at MIC (1 h) in liquid medium. Thus, it has been shown that tested oils, used even at subMIC, exhibit significant activity reducing the presence/quantity of important C. albicans virulence factors.

scholarly journals Back to Nature: Combating Candida albicans Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils

Antibiotics ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 81
Author(s):  
Ahmed M. El-Baz ◽  
Rasha A. Mosbah ◽  
Reham M. Goda ◽  
Basem Mansour ◽  
Taranum Sultana ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Essential Oils ◽  
Docking Study ◽  
New Drugs ◽  
Molecular Docking Study ◽  
Plant Essential Oils ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Analysis ◽  
Phenotypic Methods

Candida albicans is the causative agent of fatal systemic candidiasis. Due to limitations of antifungals, new drugs are needed. The anti-virulence effect of plant essential oils (EOs) was evaluated against clinical C. albicans isolates including cinnamon, clove, jasmine and rosemary oils. Biofilm, phospholipase and hemolysin were assessed phenotypically. EOs were evaluated for their anti-virulence activity using phenotypic methods as well as scanning electron microscopy (SEM) and atomic force microscopy (AFM). Among the C. albicans isolates, biofilm, phospholipase and hemolysins were detected in 40.4, 86.5 and 78.8% of isolates, respectively. Jasmine oil showed the highest anti-biofilm activity followed by cinnamon, clove and rosemary oils. SEM and AFM analysis showed reduced adherence and roughness in the presence of EOs. For phospholipase, rosemary oil was the most inhibitory, followed by jasmine, cinnamon and clove oils, and for hemolysins, cinnamon had the highest inhibition followed by jasmine, rosemary and clove oils. A molecular docking study revealed major EO constituents as promising inhibitors of the Als3 adhesive protein, with the highest binding for eugenol, followed by 1,8-cineole, 2-phenylthiolane and cinnamaldehyde. In conclusion, EOs have a promising inhibitory impact on Candida biofilm, phospholipase and hemolysin production, hence EOs could be used as potential antifungals that impact virulence factors.

scholarly journals The effect of some essential oils on pathogenes that cause eczema

2020 ◽  
Author(s):  
Meryem Karacam ◽  
Durmus Alpaslan Kaya
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Essential Oils ◽  
Staphylococcus Epidermidis ◽  
Myrtus Communis ◽  
Plant Essential Oils ◽  
Myrtus Communis L ◽  
Thymbra Spicata

In this study, the antimicrobial activity of essential oils obtained from Thymbra spicata L., Lavandula angustifolia Mill. and Myrtus communis L. on the pathogens causing eczema Staphylococcus aureus (ATCC 29213), Staphylococcus epidermidis (ATCC 12228), Escheria coli (ATCC 25922), Acinetobacter baumannii (ATCC 43498), Pseudomonas aeruginosa (ATCC 27853) ve Candida albicans (ATCC 90028) were investigated. The MIC and MBC values of the essential oils used in the study against the pathogens causing eczema were determined. As a result of the results obtained, antimicrobial activity of plant essential oils used in the study on test microorganisms was determined. Among the essential oils, it was found that the most effective essential oil was thyme followed by the lavender.

scholarly journals Experimental Germ Tube Induction in Candida albicans: An Evaluation of the Effect of Sodium Bicarbonate on Morphogenesis and Comparison with Pooled Human Serum

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Tapiwa Matare ◽  
Pasipanodya Nziramasanga ◽  
Lovemore Gwanzura ◽  
Valerie Robertson
Keyword(s):  
Candida Albicans ◽  
Human Serum ◽  
Germ Tube ◽  
Tube Formation ◽  
Joint Research ◽  
Significant Difference ◽  
Buffer Control ◽  
Tris Maleate ◽  
Microscope Slides ◽  
Germ Tubes

Objective. The potential of NaHCO3 versus human serum to induce germ tube formation in Candida albicans was investigated. Specimens. A total of 100 isolates were obtained from oral swabs of patients presenting with thrush. Approval for the study was granted by the Joint Research Ethics Committee (JREC/23/08). Method. Confirmed C. albicans isolates by routine methods were tested for germ tube induction using 5 different concentrations of Tris-maleate buffered NaHCO3 and Tris-maleate buffer control. Standard control strains included were C. albicans (ATCC 10231) and C. krusei (ATCC 6258). Microculture was done in 20 μL inoculums on microscope slides for 3 hours at 37°C. The rate of germ tube formation at 10-minute intervals was determined on 100 isolates using the optimum 20 mM Tris-maleate buffered NaHCO3 concentration. Parallel germ tube formation using human serum was done in test tubes. Results. The optimum concentration of NaHCO3 in Tris-maleate buffer for germ tube induction was 20 mM for 67% of isolates. Only 21% of isolates formed germ tubes in Tris-maleate buffer control. There was no significant difference in induction between human serum and Tris-maleate buffered NaHCO3. Conclusion. Tris-maleate buffered NaHCO3 induced germ tube formation in C. albicans isolates at rates similar to human serum.

scholarly journals Comparison of In Vitro Testing Antifungal Activity between Rosemary Essentials Oil and Fluconazol on Candida species Isolate from HIV/AIDS Patients with Candidiasis Oral

2020 ◽  
Vol 32 (3) ◽  
pp. 182
Author(s):  
Putri Intan Primasari ◽  
Dwi Murtiastutik ◽  
Pepy Dwi Endraswari ◽  
Cita Rosita Sigit Prakoeswa ◽  
Evy Ervianti
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Aids Patients ◽  
Candida Sp ◽  
Significant Difference ◽  
Rosemary Essential Oil ◽  
Hiv Aids

Background: The most common opportunistic infection obtained in patients with Human Immunodeficiency Virus (HIV)/Acquired Immune Deficiency Syndrome (AIDS) is Oral Candidiasis (OC). Currently widely reported about increased fluconazole resistance, as a therapy that is often used in patients with HIV/AIDS with OC. For this reason, effective antifungal agents are needed as alternative therapies. Several studies have reported some antifungal activity from rosemary essential oils. Purpose: The aim of this study is to evaluate the antifungal activity between rosemary essential oils and fluconazole against the isolates used by Candida sp. taken from HIV / AIDS patients with OC. Methods: This research was an experimental laboratory study with a post-test only design of 40 Candida sp. isolates from HIV / AIDS patients with OC in the Outpatient Unit and Inpatient Installation of the Infectious Disease Intermediate Care Unit (UPIPI) Dr. Soetomo General Academic Teaching Hospital, Surabaya. Examination of antifungal activity using conventional test methods with disk diffusion. Result: Disk diffusion test result revealed average inhibition zone of rosemary essential oil in Candida albicans 1,75±3,64 mm and Candida non-albicans 1,5±3,08 mm, while the average inhibition zone of fluconazole in Candida albicans 17,9±8,62 mm and Candida non-albicans 4,85±7,9 mm. There was significant difference (p <0.001) between the inhibition zone formed in Candida albicans by fluconazole and rosemary essential oil, but no significant difference of inhibition zone formed in Candida non-albicans. Conclusion: Antifungal activity fluconazole has greater than Rosemary essential oils in Candida albicans.

Effects of Sub-Minimal Inhibitory Concentrations of Some Essential Oils on Virulence Factors ofPseudomonas aeruginosa

2010 ◽  
Vol 13 (4) ◽  
pp. 465-476 ◽  
Author(s):  
P. Owlia ◽  
L. Moein Najafabadi ◽  
S. Mousavi Nadoshan ◽  
I. Rasooli ◽  
H. Saderi
Keyword(s):  
Essential Oils ◽  
Virulence Factors ◽  
Minimal Inhibitory Concentrations

scholarly journals The anti-candidal activity of Pelargonium graveolens essential oils against clinical isolates of Candida albicans

Infectio ◽  
2017 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Elaheh Mahdizadeh ◽  
Rezvan HeidaryTabar
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Clinical Isolates ◽  
Chemical Compositions ◽  
Dimorphic Fungi ◽  
Pelargonium Graveolens ◽  
Significant Difference ◽  
Life Threatening

Objectives: Candida albicans as important opportunistic dimorphic fungi can cause the life threatening infections in humans. In this study, we evaluated the anticandidal activities of six samples of Pelargonium graveolens essential oils against 31 clinical isolates of C. albicans. Materials and methods: The anti-candidal activity was performed by disc diffusion and micro-broth dilution assays. The chemical compositions of essential oils were analyzed by Gas Chromatography (GC) apparatus. Results: P. graveolens essential oil samples with citronellol (7.7-43.7%) and geraniol (19.3-48.5%) showed the same anti-candidal activity in two different methods. There is no significant difference between the inhibition zone diameters (19.3-24.1 mm), and the MIC and MFC values (1.06-1.48 and 1.5-1.72 μl/ml) of essential oil samples with different percent of citronellol and geraniol. Conclusion: Therefore, P. graveolens essential oils can be used as anti-candidal agent for further studies.

Effects of plant essential oils on growth and virulence factors of Erwinia amylovora

2019 ◽  
Vol 102 (2) ◽  
pp. 409-419 ◽  
Author(s):  
Mahdi Akhlaghi ◽  
Saeed Tarighi ◽  
Parissa Taheri
Keyword(s):  
Essential Oils ◽  
Virulence Factors ◽  
Erwinia Amylovora ◽  
Plant Essential Oils

scholarly journals Sensitivity to Fungicides and Esential Oils in Czech Isolates of Phytophthora infestans

2018 ◽  
Vol 49 (2) ◽  
pp. 69-77
Author(s):  
J. Mazáková ◽  
M. Zouhar ◽  
P. Sedlák ◽  
E. Zusková ◽  
P. Ryšánek ◽  
...  
Keyword(s):  
Essential Oils ◽  
Phytophthora Infestans ◽  
Mycelial Growth ◽  
Thymus Vulgaris ◽  
Syzygium Aromaticum ◽  
Mentha Spicata ◽  
Minimal Inhibitory Concentrations ◽  
Plant Essential Oils ◽  
Agar Method

Abstract A total of 235 Phytophthora infestans isolates were collected from five regions of the Czech Republic during the growing seasons 2012–2014 and 2016 and examined using the in vitro amended agar method for their sensitivity to metalaxyl-M (MFX), propamocarb-HCl (PCH), and dimethomorph (DMM). A majority of the isolates (50%) were sensitive to MFX. Resistant isolates were found in all four years of the survey; they represented 30% of the samples. The EC50 values of PCH in inhibiting mycelial growth of 65% of the overall isolates were higher than 100 μg ml−1, which indicates the occurrence of insensitivity to PCH in the Czech P. infestans populations. DMM was very effective, and the mycelial growth of all isolates tested was completely suppressed at the concentration of 0.1 μg ml−1. Furthermore, the efficacy of 12 plant essential oils was tested against 20 isolates of P. infestans using the in vitro amended agar method. Essential oils of Cymbopogon winterianus, Litsea cubeba, Mentha spicata, Pelargonium graveolens, Syzygium aromaticum, and Thymus vulgaris were observed to have the highest antifungal activity against P. infestans, with minimal inhibitory concentrations less than or equal to 1 μl ml−1.

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