scholarly journals The spectrum of pharmacological activity of different groups of natural compounds of laurel leaves and the choice of the optimal extractant for the extraction of essential oil from them

2021 ◽  
Vol 7 (3) ◽  
pp. 281-295
Author(s):  
Yulia E. Polousova Polousova ◽  
◽  
Dmitry I. Pisarev ◽  
Oleg O. Novikov ◽  
Rimma A. Abramovich ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Essential Oil ◽  
Pharmacological Activity ◽  
Therapeutic Potential ◽  
Ionic Current ◽  
Test Material ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Scientific Medicine ◽  
Chromatography Mass Spectrometry

Laurel noble – Laurus nobilis L., a plant whose leaves are widely used in cooking and are used in folk medicine. L. nobilis L. leaves contain more than 80 volatile components, represented mainly by monocyclic monoterpenes. The identification of more and more new therapeutic properties of the plant testifies to its incompletely undisclosed therapeutic potential. However, despite the impressive therapeutic potential of the plant, it is not used in domestic scientific medicine. The aim of the study: To describe the spectrum of types of pharmacological activity of L. nobilis L. and selection of the optimal extractant for the extraction of essential oil from the leaves of the object under study. Materials and methods: Laurel leaves were used as the test material. The essential oil from the leaves was obtained by extraction with freons, which are methoxynonafluorobutane and fluoroketone. The extractant for comparison was n-hexane. The method of studying essential oils was chromatography- mass spectrometry. Chromatography was performed on a gas chromatograph – mass spectrometer – GCMS-QP2010 Ultra, Shimadzu, Japan. Ionization is carried out in the electronic shock mode, detection by the total ionic current (SCAN) in the programmed temperature mode. Results: According to the literature, the essential oil of L. nobilis L. leaves have a pronounced antibacterial, antioxidant and antiinflammatory effect. Polyphenolic compounds of L. nobilis L. leaves are represented by flavonoids, derivatives of kaempferol and quercetin. It is believed that flavonoids are responsible for hypoglycemic, insular protective, antioxidant effects. The results of chromatography showed that the essential oil is represented mainly by 12 compounds, the dominant of which were 1.8-cineole (eucalyptol), alpha-terpenyl acetate and methyleugenol. During chromatography, it was also found that the optimal extractant is methoxynonafluorobutane, which extracts terpenoids with the highest yield. The prospects of using freons as agents for obtaining essential oil from laurel leaves have been shown. The composition of the essential oil isolated by freons from the leaves of laurel was determined by the method of gas chromatography-mass spectrometry. The results of chromatography showed that in the obtained freon extracts, 1.8-cineole (eucalyptol), alpha-terpenyl acetate and methyleugenol were dominant, which is close in composition to the native essential oil, according to literature data. Conclusion: The prospects of using freons as agents for extracting essential oil from L. nobilis L. leaves were shown. During chromatography, it was found that the optimal extractant is methoxynonafluorobutane, which extracts volatile components with the highest yield compared to fluoroketone and n-hexane; in addition, methoxyfluorobutane is non-toxic compared to n-hexane.

scholarly journals Rapid and sensitive analysis of benzyl isothiocyanate in peel, pulp, and seeds of Carica papaya Linn. by headspace gas chromatography-mass spectrometry

2021 ◽  
Vol 3 (3) ◽  
Author(s):  
Yinzheng Ma ◽  
Yingying Wen ◽  
Jinping Chen ◽  
Yunxia Zhang ◽  
Haiying Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Carica Papaya ◽  
Orthogonal Design ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Headspace Gas Chromatography ◽  
Benzyl Isothiocyanate ◽  
Chromatography Mass Spectrometry ◽  
Headspace Gas

AbstractA rapid and sensitive headspace gas chromatography-mass spectrometry (HS-GC–MS) method was established for the determination of benzyl isothiocyanate (BITC) in the peel, pulp, and seeds of Carica papaya Linn. Tween 80 solution with a concentration of 0.002% (w/v) was chosen as a headspace medium for solving the poor solubility of BITC in water without using organic solvents and ensuring high headspace efficiencies. Extraction parameters had been evaluated and optimized by using an orthogonal design with an OA9(34) table. Optimal headspace conditions were obtained when vials were equilibrated at 80 °C for 20 min at a stirring speed of 375 rpm. The calibration curve obtained by using GC–MS was linear in a concentration range of 10–320 ng/mL. The recoveries of peel, pulp, and seeds ranged from 97.3 to 100.6% with RSDs less than 3.0%. The method is simple, rapid, sensitive, and environmentally friendly. It is suitable for analyzing BITC in papaya fruit and is expected to have important application potential in the extraction of water-insoluble volatile components in foods, plants, medicines, and other samples.

scholarly journals Volatile Profiles of Five Variants of Abeliophyllum distichum Flowers Using Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry (HS-SPME-GC-MS) Analysis

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 224
Author(s):  
Yeong-Geun Lee ◽  
Won-Sil Choi ◽  
Seung-Ok Yang ◽  
Jeon Hwang-Bo ◽  
Hyoun-Geun Kim ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Morphological Characteristics ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Headspace Solid Phase Microextraction ◽  
Chromatography Mass Spectrometry ◽  
Abeliophyllum Distichum

Abeliophyllum distichum (Oleaceae), which is the only species in the monotypic genus and is grown only on the Korean peninsula, has a high scarcity value. Its five variants (white, pink, round, blue, and ivory) have different morphological characteristics in terms of the color of petals and sepals or shape of the fruits. Despite its high value, there has been no study on variant classification except in terms of their morphological characteristics. Thus, we performed a volatile component analysis of A. distichum flowers and multivariate data analyses to reveal the relationship between fragments emitted from five variants of A. distichum flowers with their morphological characteristics. As a result, 66 volatile components of this plant were identified by headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS), showing unique patterns for each set of morphological characteristics, especially the color of the petals. These results suggest that morphological characteristics of each variant are related to the volatile composition.

Identification by Gas Chromatography-Mass Spectrometry of 150 Compounds in Propolis

1991 ◽  
Vol 46 (1-2) ◽  
pp. 111-121 ◽  
Author(s):  
W. Greenaway ◽  
J. May ◽  
T. Scaysbrook ◽  
F. R. Whatley
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Alcoholic Extract ◽  
Headspace Volatiles ◽  
Chromatography Mass Spectrometry ◽  
First Time

Abstract Propolis was analyzed by gas chromatography-mass spectrometry for both its headspace volatiles and for the less volatile components of its alcoholic extract (propolis balsam). 181 peaks were located of which 171 representing 150 compounds were identified, including 28 identified in propolis for the first time. The majority of compounds were typical of poplar bud exudate.

scholarly journals Volatile Constituents of the Leaves of Kalanchoe pinnata From the Venezuelan Andes

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1984270
Author(s):  
Ysbelia Obregón-Díaz ◽  
Alida Pérez-Colmenares ◽  
Karelys Obregón-Alarcón ◽  
Rosa Aparicio-Zambrano ◽  
Luis Rojas-Fermín ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Constituents ◽  
Chromatography Mass Spectrometry ◽  
Kalanchoe Pinnata

The essential oil from the leaves of Kalanchoe pinnata (Lam.) Pers. (Crassulaceae), collected in the Venezuelan Andes, was obtained by hydrodistillation and its composition determined by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). Nineteen compounds were identified, representing 95.2% of the oil. The most abundant components were α-curcumene (44.7%), 1-octen-3-ol (18.1%), β-curcumene (4.9%), and β-caryophyllene (3.7%).

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