scholarly journals Semen analysis of subfertility caused by testicular carcinoma

2020 ◽  
Author(s):  
Behrooz Ghasemi ◽  
Alimohammad Mosadegh Mehrjardi ◽  
Carolyn Jones ◽  
Nasrin Ghasemi
Keyword(s):  
Testicular Cancer ◽  
Semen Quality ◽  
Semen Analysis ◽  
Correct Diagnosis ◽  
Sperm Concentration ◽  
Germ Cell Tumors ◽  
Normal Morphology ◽  
Semen Parameter ◽  
Individual Traits ◽  
Testicular Carcinoma

Background: Infertility is a common problem in testicular cancer. Affected men often decide to undergo sperm banking before chemo/radiotherapy. The cumulative effects of therapy can considerably reduce fertility. Objective: Testicular cancers impair fertilizing ability, even before diagnosis. This study tries to verify individual traits and semen quality in patients with testicular cancer. Materials and Methods: This observational study analyzed 190 semen of patients with testicular cancer (16 to 47 yr old) referred to the sub-fertility laboratory at the St. Mary hospital for semen banking prior to treatment carcinoma. Several aspects of their semen analyses were examined. The cases were divided into four different categories: seminoma, teratoma, mixed germ cell tumors and others. Results: The results showed that 23 cases were azoospermic, and 13 of the patients who were not azoospermic, their sperm of “normal” morphology were too few to count. Among patients that could produce spermatozoa, 59.4% had a sperm concentration of < 20 × 106/ml. The mean of “motility excellent” and “sluggish” taken together in all the cases was 47.2%. More than 92% of the patients had an abnormal morphology. The morphology of sperm is the most sensitive semen parameter that is affected by testicular carcinoma. Conclusion: Abnormal spermatogenesis is seen in most patients with testicular cancer before treatment with radiation, chemotherapy, or surgery. The causes of poor semen quality in cancer patients are not well-understood, but the patients with impaired spermatogenesis should have precise examination to find out the correct diagnosis of problem and preserve the fertility before any treatment. Key words: Infertility, Testicular cancer, Semen analyses, Spermatogenesis.

Persistence of platinum in semen of cisplatin-treated survivors of advanced testicular cancer.

2019 ◽  
Vol 37 (7_suppl) ◽  
pp. 519-519 ◽  
Author(s):  
Eoghan Ruadh Malone ◽  
Jeremy Howard Lewin ◽  
Susan Lau ◽  
Robert James Hamilton ◽  
Aaron Richard Hansen ◽  
...  
Keyword(s):  
Testicular Cancer ◽  
Semen Quality ◽  
Sperm Count ◽  
Semen Analysis ◽  
Pearson Correlation ◽  
Germ Cell Tumors ◽  
Post Treatment ◽  
Platinum Concentration ◽  
Median Serum ◽  
Advanced Testicular Cancer

519 Background: Even in patients (pts) with widely metastatic germ cell tumors cure rates are high with cisplatin based chemotherapy. Survivors of testicular cancer often have impaired gonadal function possibly related to chemotherapy. Most pts develop temporary azoospermia with recovery in about 50% after 2 years and 80% after 5 years. Platinum persists in blood in these pts, however, it is not known whether platinum also persists in semen. Methods: Pts who completed cisplatin > 3 months previously were enrolled. Age, total cisplatin dose and date of completion of treatment were collected. A semen sample was collected from each patient for analysis to assess semen quality. A blood sample was collected to assess FSH/LH/testosterone/creatinine. Serum and semen platinum levels were measured using HPLC-tandem mass spectrometry. Results: Between 11/2017 and 09/2018, 9 pts (median age 32 years; range: 18-52) were enrolled to the study, all were treated with standard Bleomycin, Etoposide, Cisplatin regimen. Blood samples were collected from 7 pts, and semen samples from 4 pts. Median total cisplatin dose given was 658 mg (range: 570-780). Median serum platinum concentration was 0.395 ng/mL (range: 0.10-0.94) at a median of 10 months (range 4.5-13) post completion of treatment. Median semen platinum concentration was 1.06 ng/mL (range: 0.25-1.47) at a median of 10 months (range: 6-14) post completion of treatment. Semen platinum levels were associated with total cisplatin dose administered (Pearson correlation, r = 0.645) and time from completion of treatment (Pearson correlation, r = 0.386). In all 4 pts with matched samples, the semen platinum level was higher than the serum platinum level. Semen analysis showed 2 pts were azoospermic post treatment, 1 of these was also azoospermic prior to treatment; 1 pt had normal semen analysis post treatment and 1 had a low sperm count post treatment. Conclusions: This is the first study demonstrating that platinum persists in semen > 6 months post completion of cisplatin-containing chemotherapy. Platinum levels are higher in semen than in blood following treatment. Our preliminary findings may have important implications for reproductive health of survivors of advanced testicular cancer.

Impaired Testicular Function in Patients With Carcinoma-In-Situ of the Testis

1999 ◽  
Vol 17 (1) ◽  
pp. 173-173 ◽  
Author(s):  
Peter Meidahl Petersen ◽  
Aleksander Giwercman ◽  
Steen W. Hansen ◽  
Jørgen G. Berthelsen ◽  
Gedske Daugaard ◽  
...  
Keyword(s):  
Testicular Cancer ◽  
Germ Cell ◽  
Leydig Cell ◽  
Carcinoma In Situ ◽  
Cell Function ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Testicular Dysfunction ◽  
Leydig Cell Function

PURPOSE: To elucidate the biologic association between germ cell neoplasia and testicular dysfunction, through investigation of Leydig cell function and semen quality in men with carcinoma-in-situ (CIS) of the testis. PATIENTS AND METHODS: We examined two groups of men, unilaterally orchidectomized for testicular cancer. Biopsy of the contralateral testis had showed CIS in a group of 24 patients and no evidence of CIS in the other group of 30 patients. Semen quality and serum levels of testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were compared in these two groups of men after orchidectomy but before further treatment for testicular cancer. RESULTS: Significantly higher LH levels (median, 8.1 IU/L v 4.8 IU/L; P < .001) and generally lower testosterone levels (median, 12.5 nmol/L v 15.5 nmol/L; P = .13) were found in the CIS group. The proportion of patients with Leydig cell dysfunction was higher in the group of patients with CIS (11 of 24) than in the group of patients without (two of 30) (P = .01). Sperm concentration and total sperm count were significantly lower (P < .001) in patients with CIS (median, 0.03 × 106/mL and 0.10 × 106, respectively) than in patients without (median, 9.1 × 106/mL and 32 × 106, respectively), whereas the levels of FSH were significantly higher (P < .001) in the former group of men (median, 19.6 IU/L v 9.0 IU/L). CONCLUSION: Not only spermatogenesis but also Leydig cell function is impaired in testes with CIS. This impairment could be due to common factors in the pathogenesis of germ cell neoplasm and testicular dysfunction. Alternatively, CIS cells may have a negative impact on Leydig cell function.

scholarly journals Influence of Preoperative Pain Duration on Microsurgical Varicocelectomy Outcomes

2013 ◽  
Vol 2013 ◽  
pp. 1-4
Author(s):  
Mustafa Gökhan Köse ◽  
Kadir Önem ◽  
Mehmet Çetinkaya ◽  
Erkan Karadağ ◽  
Emre Arpali
Keyword(s):  
Semen Quality ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Sexual Abstinence ◽  
Preoperative Pain ◽  
Intergroup Comparison ◽  
Pain Duration ◽  
Group 2 ◽  
Grade 3 ◽  
Group 1

Objective. To investigate the question of whether duration of pain before surgery ultimately affects sperm parameters after varicocelectomy.Methods. Fifty patients with painful grade-3 varicocele were investigated prospectively. The patients were divided into two groups according to their symptom period. The patients having had grade-3 varicocele for less than 1 year were included in Group-1 (Ge,n=25). Twenty-five patients who had painful grade-3 varicocele for more than 1 year (Gs,n=25) were classified in Group-2. Semen analysis was performed after 3 days of sexual abstinence twice a month. Total sperm concentration (TSC), rapidly progressive motility (SPa), and slow or sluggish motility (SPb) rates were noted. Pain was evaluated by using 10 cm visual analogue scale (VAS).Results. Postoperative TSC and %SPb were significantly higher in both groups (P=0.01). There was no difference between two groups for preoperative and postoperative TSC, %SPa, % and SPb values. VAS significantly declined in both groups (P=0.005). This postoperative decline was not significant for intergroup comparison.Conclusions. Our results show that increase in semen quality and decrease in the pain after microsurgery varicocelectomy do not depend on the duration of the preoperative pain.

Seasonal patterns of LH, testosterone and semen quality in the Northern pintail duck (Anas acuta)

2000 ◽  
Vol 12 (4) ◽  
pp. 229 ◽  
Author(s):  
L. M. Penfold ◽  
D. E. Wildt ◽  
T. L. Herzog ◽  
W. Lynch ◽  
L. Ware ◽  
...  
Keyword(s):  
Breeding Season ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Day Length ◽  
Seasonal Patterns ◽  
Sperm Production ◽  
Normal Morphology ◽  
Northern Pintail ◽  
Sperm Trait ◽  
Percent Normal

This study characterized seasonal changes in circulating LH and testosterone and in semen production and quality in the Northern pintail duck. Plasma LH and testosterone were measured in blood samples collected weekly throughout the year from eight males exposed to natural fluctuations in day length and temperature. Semen quality was evaluated weekly in these same males from April–June, the months when spermatozoa were produced. Semen quality (based on sperm concentration and normal morphology) peaked 0–2 weeks after sperm production onset and decreased sharply before sperm production cessation in late June. Nadir LH concentrations were measured in July and August with peak LH observed in May and November. There were clear seasonal patterns in circulating testosterone with July–September values being less (P<0.05) than October–December which, in turn, were less (P<0.05) than January–March. Maximal circulating testosterone (P<0.05) occurred during April–June, coincident with semen production. Weekly circulating LH during the breeding season was directly related to testosterone concentrations (P<0.01), but was not correlated to any specific semen or sperm trait (P>0.05). Testosterone concentrations throughout the breeding season were correlated (P<0.05) to total numbers of spermatozoa produced (volume cell concentration) and percent normal sperm morphology. In summary, the Northern pintail experiences seasonal hormone fluctuations, with maximum circulating testosterone coinciding with peak ejaculate quality reflected by the production of high numbers of morphologically normal spermatozoa.

scholarly journals Incidence and impact of hyperviscosity on sperm parameters of Malawian men seeking assisted reproduction

2020 ◽  
Vol 20 (1) ◽  
pp. 1-3
Author(s):  
Fanuel Lampiao ◽  
Joseph Chisaka
Keyword(s):  
Male Infertility ◽  
Sperm Motility ◽  
Volume Concentration ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Infertility Treatment ◽  
Normal Morphology ◽  
Progressive Motility ◽  
Accessory Sex Glands ◽  
Semen Volume

Background: Seminal hyperviscosity has been shown to be associated with male infertility. The aim of this study was to assess the prevalence of hyperviscosity in semen of Malawian males seeking infertility treatment. Methods: A total of 120 men visiting our laboratory for fertility assessment donated semen samples. The semen samples were assessed for hyperviscosity, volume, concentration, total motility, progressive motility, viability, and morphology. Results: Out of the 120 samples analyzed, 34 samples were hyperviscous representing 28.3%. No significant statistical differ- ence in semen volume between samples with normal viscosity compared to those with hyperviscosity (p>0.05). Sperm concen- tration, progressive motility, total motility, viability, and normal morphology were significantly higher in the normal viscosity group when compared to the abnormal viscocity group (p<0.05). Conclusion: Hyperviscosity affects a significant number of men in Malawi and may be the cause of decreased fertility as it was associated with poor sperm concentration, total motility, progressive motility, viability, and morphology. Keywords: Viscosity; sperm motility; accessory sex glands; infertility; semen; semen analysis; spermatozoa.

scholarly journals Seasonal variations in quantitative and qualitative sperm characteristics in fertile and subfertile stallions

2020 ◽  
Vol 63 (1) ◽  
pp. 145-154 ◽  
Author(s):  
Yara Suliman ◽  
Frank Becker ◽  
Armin Tuchscherer ◽  
Klaus Wimmers
Keyword(s):  
Breeding Season ◽  
Seasonal Variations ◽  
Semen Quality ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Computer Assisted ◽  
Normal Sperm ◽  
Straight Line ◽  
Significant Seasonal Variation

Abstract. Horses are seasonal breeders with a natural breeding season beginning in spring and extending through midsummer. In this study, quantitative and qualitative parameters of chilled stallion semen were compared between fertile and subfertile stallions and between the breeding and the non-breeding season. Semen quality parameters compared included ejaculate volume, sperm concentration, total sperm number, sperm morphology, and computer-assisted semen analysis (CASA)-derived sperm movement characteristics obtained from two groups of warmblood stallions (n=8; four fertile stallions and four subfertile stallions), which differ in the seasonal pregnancy rate 80 %–90 % (fertile) vs. 40 %–60 % (subfertile). A total of 64 ejaculates were collected from the stallions (n=8; four in the breeding season and four in the non-breeding season of each stallion). No significant differences in the semen quality parameters between the fertile and the subfertile stallions in the non-breeding season were observed. However, in the breeding season the proportion of morphologically normal sperm, total motility, progressive motility, average path velocity (VAP), and curvilinear velocity (VCL) were significantly higher in the fertile group (P<0.05) when compared with the subfertile group. In addition, a significant seasonal variation in the proportion of morphological normal sperm was found in the fertile group between the breeding and the non-breeding season (P<0.05). Moreover, significant seasonal variations (P<0.05) in CASA parameters of mean VAP, straight line velocity (VSL), and beat-cross frequency (BCF) were observed in the fertile and the subfertile stallions, which tended to be lower in the non-breeding season. In conclusion, differences between the fertile and the subfertile stallions were observed only in the breeding season, and a few of CASA-derived parameters seemed to be significantly lower during the non-breeding season in both the fertile and the subfertile stallions.

scholarly journals Abnormal semen parameters among males in infertile couples: a cross sectional study from a tertiary care centre

2020 ◽  
Vol 9 (8) ◽  
pp. 3398
Author(s):  
Jyoti Garg ◽  
Rachana Meena ◽  
Shailaja Shukla ◽  
Sunita Sharma ◽  
Riva Choudhury
Keyword(s):  
Semen Quality ◽  
Semen Analysis ◽  
Tertiary Care ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Medical Attention ◽  
Male Factor Infertility ◽  
Male Factor ◽  
Cross Sectional ◽  
Infertile Couples

Background: In India, the prevalence of primary infertility ranges from 3.9% to 16.8%. Male factor contributes 40-50% of this. Male factor infertility is indicated by decreased sperm concentration, reduced motility, vitality or abnormal sperm morphology. Semen analysis is the single most important investigation to detect male factor infertility. The aim of this study was to analyse the prevalence of abnormal semen parameters among males in infertile couples and their association with contributing factors.Methods: This cross-sectional hospital-based study was carried out in the Department of Pathology at Lady Hardinge Medical College and Smt. Sucheta Kriplani Hospital. A total of 400 cases were analyzed during a period of 6 months. Detailed history of the couple was taken. Semen analysis was done using automated semen analyzer (SQA-vision) after 3 days of abstinence according to the WHO 5th edition 2010 guidelines. The results were analysed using excel sheet and SPSS software.Results: In the present study, 122 cases (30.5%) out of 400 cases had abnormal semen parameters. Most common abnormality detected was asthenozoospermia (14.3%) followed by oligozoospermia (13.8%), azoospermia (10.5%) and teratozoospermia (10.5%). There was significant association of alcohol intake, obesity and trauma with abnormal semen parameters.Conclusions: Asthenozoospermia was the most common abnormality noted in this study. Lifestyle modifications along with timely medical attention in male partners of infertile couples can improve the semen quality.

scholarly journals The Association Between Abstinence Period and Semen Parameters: Results in Normal Samples and Different Sperm Pathology

2021 ◽  
Author(s):  
Min Xie ◽  
Silvan Hämmerli ◽  
Kerstin Blickenstorfer ◽  
Brigitte Leeners
Keyword(s):  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Sexual Abstinence ◽  
Normal Morphology ◽  
Few Data ◽  
Total Motile Sperm Count ◽  
Abstinence Period

Abstract Purpose: The length of sexual abstinence seems to influence sperm quality. However, few data on the relevance of abstinence time in pathological sperm samples are available. With our study, we look for associations of abstinence length and semen quality. Methods: We studied semen samples from 4423 men undergoing fertility evaluation. Sperm concentration, percentage of progressively motile spermatozoa, total motile sperm count, percentage of spermatozoa with normal morphology, were compared after each day and 0-2, 3-7 and >7 days of abstinence. Results: We found that a longer abstinence time was related to higher sperm concentration in normal semen samples (P<0.001) and in semen samples with any sperm pathology (P<0.001, P=0.004) with the exception of oligozoospermia (P=0.125). Longer abstinence time was also associated with significantly reduced progressive motility in normal samples (P<0.001) and in cases of teratozoospermia (P<0.001). In normal samples a higher percentage of sperm were morphologically normal after a shorter abstinence period (P=0.03); in oligoasthenoteratozoospermia (OAT) samples, this was the case after a longer abstinence period (P=0.013). Conclusion: A longer abstinence time is associated with higher sperm concentration, whereas sperm motility is optimal after shorter abstinence times; results on morphology are controversial. The recommendation on abstinence time needs to be adjusted in relation to the parameter that needs to be improved.

scholarly journals Correlation of Sperm DNA Fragmentation Index With Seminal Plasma Biochemical Index and Semen Parameters in Infertile Men

2020 ◽  
Author(s):  
Kang-Sheng Liu ◽  
Xiao-Dong Mao ◽  
Feng Pan ◽  
Xinyuan Yang
Keyword(s):  
Survival Rate ◽  
Dna Fragmentation ◽  
Semen Quality ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Group Iii ◽  
Group I ◽  
Sperm Dna ◽  
Semen Volume ◽  
Sperm Survival

Abstract BackgroundAccording to world Health Organization guidelines, semen analysis by testing routine parameters is the main method for assessing male fertility.In general, routine semen analysis makes only limited predictions about a man's reproductive potential and is not always able to explain why he is infertile. In fact, many male infertility cases are caused by sperm DNA defects ,which routine semen quality analyses fail to detect.The relationship between sperm DFI , sperm parameters and their diagnostic value were analyzed and evaluated by observing the seminal parameters of infertile patients without accessory gonadal infection.MethodsSpecimens of 151 cases were collected from infertile patients who visited the male department of STD and reproductive specialty clinic of our hospital from August 2018 to September 2019. SCD test was performed to measure the DNA fragmentation in native. The routine semen analysis was performed with a semen quality detection system (WLJY-9000, Beijing Weili New century Science & Tech Dev .Co.Ltd) and supporting reagents. Seminal plasma malondialdehyde (MDA), and total antioxidant capacity (TAC) were assessed. Fructose(Fru) 、a-glucosidase (,a-glu), and zinc (Zn) levels are quantitatively detected by kehua-310, a fully automated biochemical tester provided by Nanjing Xindibio.ResultsAccording to DFI level, there were 31 cases in group I (DFI≤15%), 81 cases in group II (15% < DFI < 30%), and 39 cases in group III (DFI≥30%). Compared with group II, there were significant differences in sperm survival rate, PR% and Fru by non-parametric test (Z = -2.16 -2. 43. - 2. 20,respectively,P < 0. 05). There were significant differences in sperm survival rate and PR% between group I and group III (t = 4. 32, 4.25, respectively, P< 0.01). Compared with group III, there were significant differences in sperm survival rate and PR% by non-parametric test (Z= -3. 26, -3. 50, respectively).Sperm DFI was negatively correlated with sperm survival rate and PR%(R=-0.56,-0.46,P<0.01). DFI was positively correlated with MDA content (R=0.42, P<0.01) and negatively correlated with TAC (r=-0.40, P<0.01).There was no correlation with age ,abstinence days, semen volume, sperm concentration, percentage of normal form sperm, Fru, a-Glu, Zn (R= 0. 15, 0. 05,0.03,-0.03, -0.2, -0.16,- 0.20, 0.03, 0.15, p > 0.05).ConclusionThe survival rate and PR% of sperm decreased significantly with the increase of DFI level, antioxidant can decrease the rate of DNA fragmentation, antioxidant can decrease the rate of DNA fragmentation. Semen volume and sperm concentration were mainly related to the secretion volume of accessory gonads and total sperm count, but no correlation was found between them and DFI.

scholarly journals Processed meat intake in relation to semen quality indicators among men attending a fertility clinic

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Anna Danielewicz ◽  
Katarzyna Przybyłowicz ◽  
Jakub Morze
Keyword(s):  
Quality Indicators ◽  
Semen Quality ◽  
Negative Impact ◽  
Sperm Count ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
World Health ◽  
Processed Meat ◽  
Cross Sectional ◽  
Meat Intake

AbstractIt is estimated that in Central and Eastern Europe 8–12% of men are infertile, and in the case of 20% of infertile couples in the region, indicates a 56% part of the malefactor in the occurrence of infertility. Numerous studies have shown that a healthy diet with a high intake of fish and seafood, poultry, vegetables, fruits, legumes, through the high content of antioxidants are associated with better parameters of semen quality and reduced risk of asthenozoospermia. Men's diets may affect spermatogenesis as reflected in semen quality indicators, but the literature on the relation between meat intake and semen quality is limited. Our objective was to prospectively examine the relation between meats including red, poultry, proceesed and fish and indicators of semen quality. Men in subfertile couples presenting for evaluation at the Olsztyn Hospital Fertility Center were invited to participate in an ongoing study of environmental factors and fertility. This cross-sectional study was carried out in 208 men aged 18–55 years from Northern-Eastern Poland. Semen samples were collected at the clinic and analyses were performed by an experienced technician. Semen microscopic measurements were determined with the use of computer-aided semen analysis (CASA) and evaluated according to the World Health Organization (WHO) guidelines. A total of men completed a validated food-frequency questionnaire and subsequently provided semen samples. We used linear mixed regression models to examine the relation between meat intake and semen quality indicators (total sperm count, sperm concentration, progressive motility, morphology, and semen volume) while adjusting for potential confounders. After adjustment for age, BMI, physical activity, residence place and education, one serving increase in intake of processed meat was associated with a 56% higher risk of abnormal progressive sperm motility (OR = 1.56 95%CI:1.09–2.21). Consuming processed meat may have a negative impact on sperm counts and morphology. Potentially, these products may be a significant source of saturated and trans fatty acids, xenobiotics, mainly xenoestrogen, and steroids. Is need to clarify the potential effect of meat products, especially processed meat, on male reproductive potential in RCTs or longitudinal studies. Future findings may be useful to establish a more precise recommendation connected with red and processed meat intake in the context of reproductive health.

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