Studying Onset and Evolution of Agricultural Drought in Mekong River Basin through Hydrologic Modeling

Departures in precipitation from the normal are the cause of the onset of agricultural drought. In this study, we aim to identify extreme precipitation deficits using an index called Percent Normal (PN). We applied the proposed PN index to the agriculturally productive Mekong River Basin (MRB) to evaluate the propagation of precipitation deficits into agricultural drought based on the change in slope and mean of the precipitation, soil moisture and evapotranspiration anomalies. The results of the study showed the proposed PN index identified historical droughts in the years 1992, 1997–1998 and 2000–2006 in MRB; of these, 1992 was shown to be the longest drought, which lasted from the 43rd week (October) of 1991 to the 49th week (December) of 1994. The short-term but extreme drought was identified to occur in 2005 with below-normal precipitation that lasted for more than a year. An immediate effect of precipitation deficit was observed in evapotranspiration (ET) and soil water for agricultural (Thailand) and forested regions (Parts of Cambodia) of the basin with <5 weeks lag. We conclude that the drought indices adopted in this study are suitable to identify the small and long-term drought events, which will facilitate the development of a drought-resilient agricultural production system.

Perioperative Normal Saline Administration and Delayed Graft Function in Patients Undergoing Kidney Transplantation: A Retrospective Cohort Study

Background Perioperative normal saline administration remains common practice during kidney transplantation. The authors hypothesized that the proportion of balanced crystalloids versus normal saline administered during the perioperative period would be associated with the likelihood of delayed graft function. Methods The authors linked outcome data from a national transplant registry with institutional anesthesia records from 2005 to 2015. The cohort included adult living and deceased donor transplants, and recipients with or without need for dialysis before transplant. The primary exposure was the percent normal saline of the total amount of crystalloids administered perioperatively, categorized into a low (less than or equal to 30%), intermediate (greater than 30% but less than 80%), and high normal saline group (greater than or equal to 80%). The primary outcome was the incidence of delayed graft function, defined as the need for dialysis within 1 week of transplant. The authors adjusted for the following potential confounders and covariates: transplant year, total crystalloid volume, surgical duration, vasopressor infusions, and erythrocyte transfusions; recipient sex, age, body mass index, race, number of human leukocyte antigen mismatches, and dialysis vintage; and donor type, age, and sex. Results The authors analyzed 2,515 records. The incidence of delayed graft function in the low, intermediate, and high normal saline group was 15.8% (61/385), 17.5% (113/646), and 21% (311/1,484), respectively. The adjusted odds ratio (95% CI) for delayed graft function was 1.24 (0.85 to 1.81) for the intermediate and 1.55 (1.09 to 2.19) for the high normal saline group compared with the low normal saline group. For deceased donor transplants, delayed graft function in the low, intermediate, and high normal saline group was 24% (54/225 [reference]), 28.6% (99/346; adjusted odds ratio, 1.28 [0.85 to 1.93]), and 30.8% (277/901; adjusted odds ratio, 1.52 [1.05 to 2.21]); and for living donor transplants, 4.4% (7/160 [reference]), 4.7% (14/300; adjusted odds ratio, 1.15 [0.42 to 3.10]), and 5.8% (34/583; adjusted odds ratio, 1.66 [0.65 to 4.25]), respectively. Conclusions High percent normal saline administration is associated with delayed graft function in kidney transplant recipients. Editor’s Perspective What We Already Know about This Topic What This Article Tells Us That Is New

Male traits and herd reproductive capability in tropical beef cattle. 2. Genetic parameters of bull traits

A total of 4063 young bulls of two tropical genotypes (1639 Brahman and 2424 Tropical Composite) raised in northern Australia were evaluated for a comprehensive range of production and reproduction traits up to 24 months of age. Prior to weaning, peripheral blood concentrations of luteinising hormone (LH) and inhibin were measured at 4 months of age. At weaning (6 months) blood insulin-like growth factor-1 (IGF-I) and flight time were recorded. Body composition traits of fat depth and eye-muscle area were determined by ultrasonography at 15 months of age when additional measurements of liveweight, hip height and body condition score were recorded. Bull breeding soundness was evaluated at ~12, 18 and 24 months of age when measurements of scrotal circumference, sheath score, semen mass activity, progressive motility of individual sperm and percent morphologically normal sperm were recorded. Magnitude of heritability and genetic correlations changed across time for some traits. Heritability of LH, inhibin, IGF-I and of 18-month scrotal circumference, mass activity, progressive motility and percent normal sperm was 0.31, 0.74, 0.44, 0.75, 0.24, 0.15 and 0.25, respectively, for Brahmans and 0.48, 0.72, 0.36, 0.43, 0.13, 0.15 and 0.20, respectively, for Tropical Composites. Inhibin and IGF-I had moderate genetic association with percent normal sperm at 24 months in Brahmans but low to negligible associations in Tropical Composites. Body condition score in Brahmans and sperm motility (mass and individual) traits in both genotypes had moderate to strong genetic correlation with percent normal sperm and may prove useful candidates for indirect selection. There is scope to increase scrotal circumference by selection and this will be associated with favourable correlated responses of improved semen quality in both genotypes. The lack of genetic antagonism among bull traits indicates that selection for improved semen quality will not adversely affect other production traits.

Seasonal patterns of LH, testosterone and semen quality in the Northern pintail duck (Anas acuta)

This study characterized seasonal changes in circulating LH and testosterone and in semen production and quality in the Northern pintail duck. Plasma LH and testosterone were measured in blood samples collected weekly throughout the year from eight males exposed to natural fluctuations in day length and temperature. Semen quality was evaluated weekly in these same males from April–June, the months when spermatozoa were produced. Semen quality (based on sperm concentration and normal morphology) peaked 0–2 weeks after sperm production onset and decreased sharply before sperm production cessation in late June. Nadir LH concentrations were measured in July and August with peak LH observed in May and November. There were clear seasonal patterns in circulating testosterone with July–September values being less (P<0.05) than October–December which, in turn, were less (P<0.05) than January–March. Maximal circulating testosterone (P<0.05) occurred during April–June, coincident with semen production. Weekly circulating LH during the breeding season was directly related to testosterone concentrations (P<0.01), but was not correlated to any specific semen or sperm trait (P>0.05). Testosterone concentrations throughout the breeding season were correlated (P<0.05) to total numbers of spermatozoa produced (volume cell concentration) and percent normal sperm morphology. In summary, the Northern pintail experiences seasonal hormone fluctuations, with maximum circulating testosterone coinciding with peak ejaculate quality reflected by the production of high numbers of morphologically normal spermatozoa.

Intracytoplasmic sperm injection--clinical results from the reproductive medicine unit, Adelaide

The clinical results of 391 cycles of intracytoplasmic sperm injection (ICSI) performed between June 1993 and July 1994 are presented in this report. A total of 4797 oocytes were collected, of which 3792 were injected. Of these, 2603 (69%) fertilized, with normal and three pronuclear fertilization rates of 65% and 4% respectively. About 6% of the oocytes were destroyed while denuding and during ICSI. There were 373 (95%) embryo transfers from which 119 pregnancies arose, giving pregnancy rates of 32% per transfer and 30% per cycle, and an implantation rate of 15% per embryo. Of the pregnancies, 98 (82%) were ongoing. Supernumerary embryos were frozen in 44% of the cycles and 61 subsequent transfers of 130 frozen-thawed embryos produced 11 pregnancies (18%). Only 47 (12%) patients had less than 50% of their oocytes fertilized (mean 31%) after ICSI, and of these, 8 had no fertilization of 13 eggs. Nevertheless, 37 of these 47 patients had an embryo transfer and 9 achieved a pregnancy with an implantation rate of 14% per embryo. The percent normal sperm morphology weakly correlated with percent fertilization (r2 = 0.027, P < 0.02) but not with the implantation rate (r2 = 0.003, P > 0.05). Fifty-nine patients with only occasional motile sperm in the ejaculate and 23 patients in whom epididymal sperm were aspirated were treated. The fertilization rates (66% and 70% respectively) and pregnancy rates per transfer (32% and 24% respectively) were comparable in these two subgroups. The overall ICSI results were also compared with 515 cycles of routine in vitro fertilization (IVF) which were performed at the same time.(ABSTRACT TRUNCATED AT 250 WORDS)

EFFECTS OF SEED SIZE ON LIMA BEAN GROWTH AND DEVELOPMENT

Objectives of this study were to determine the effects of lima bean seed size differences and a short chilling period after planting on seedling emergence rate, seedling abnormalities and vigor. Individual seeds of 'Jackson Wonder' lima bean were weighed and placed into one of five size classes: 24-33. 36-41. 44-49, 52-57, and 60-73 g per 100 seed. Seed of each size class were germinated at a constant 23-26C or chilled at 8C for 24 hrs and then moved to 23-26C conditions for the remainder of the study. A 24 hr chilling period after planting had a detrimental effect on subsequent lima bean seedling emergence only from 8 through 11 days after planting. Plant fresh and dry weights were significantly less for the chilled seed treatment. Temperature treatments had no effect on percent normal and abnormal seedlings or primary leaf area. No differences in seedling emergence number or rate were found among seed size classes. Smaller seedclasses had significantly fewer normal and more abnormal seedlings than larger seed size classes. The largest seed class produced seedlings with about two times more fresh and dry weights and leaf area than those from the smallest seed weight class. Plant fresh and dry weights and leaf areas from all seed size classes were significantly different from each other.

EVALUATION OF BULL FERTILITY IN MULTIPLE-SIRE MATING AT PASTURE

Pregnancy rates and calving dates of beef cows in four multiple-sire breeding herds in 1980 and 1981 were used to evaluate bull fertility. The bulls were from Hereford, Beef Synthetic and Dairy Synthetic breed groups, and were measured for scrotal circumference, scored for libido and evaluated for semen characteristics 1 wk before the commencement of breeding. Three yearling bulls were assigned to approximately 70 cows in each of the four herds (a total of 12 bulls) during a 60-d breeding season. The progeny of each sire were identified by blood typing. There was one bull in each breeding herd which sired at least 50% of the calves. The average age was higher in bulls with the maximum fertility (P < 0.05) than those with the minimum fertility within each herd. The bulls with minimum fertility within each herd tended (P = 0.08) to lose more weight during the period between yearling and breeding man those with higher fertility. Age of bull and percent normal spermatozoa were the only traits which were associated (P < 0.05) with bull fertility when data were pooled over the herds. Scrotal circumference, sperm volume, preweaning average daily gain, weaning weight and daily gain in the period between yearling and breeding showed positive but insignificant correlation with bull fertility. The measures of libido did not show any association with fertility. Key words: Cattle (beef), bull fertility, multiple-sire mating

Stability of Antibiotic Admixtures Frozen in Minibags

This study investigated the stability of frozen antibiotic admixtures prepared in minibags containing 50 ml of 0.9 percent normal saline and 5 percent dextrose in water. The minibag antibiotic admixtures studied were ampicillin sodium 1 g, carbenicillin disodium 2 g, cephalothin sodium 1 g, cloxacillin sodium 1 g, cefazolin sodium 1 g, gentamicin sulfate 50 mg, penicillin G potassium 2 million units, erythromycin gluceptate 500 mg and rolitetracycline base 275 mg. The stability of each antibiotic was determined by a quantitative microbiological agar gel diffusion assay. The minibags were frozen within an hour of the admixtures being prepared. During storage in a freezer at −20 °C (–4 °F), the minibags were placed in a storage module to reduce unnecessary handling. Assays were conducted on minibags which had been frozen for 24 hours, 7 days and 30 days. All thawed samples were re-assayed after storage at 5–6 °C (21–23 °F) for 21 hours. The results of the study indicated that minibag admixtures of carbenicillin disodium, cephalothin sodium, cloxacillin sodium, gentamicin sulfate, penicillin G potassium, erythromycin gluceptate, cefazolin sodium and rolitetracycline base in the concentrations tested can be frozen for 30 days without a significant loss in activity. These antibiotics were also stable when thawed and stored at 5–6 °C (21–23 °F) for 21 hours. Ampicillin sodium admixtures were not stable when frozen. A handling procedure for the frozen minibags is described. Specific recommendations are also included for freezing and thawing the minibags and in the use of a specially designed storage module.