scholarly journals Cross-platform genomic identification and clinical validation of breast cancer diagnostic biomarkers

Aging ◽  
2021 ◽  
Author(s):  
Dongdong Liu ◽  
Beibei Li ◽  
Xiaoshun Shi ◽  
Jiexia Zhang ◽  
Allen Menglin Chen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Clinical Validation ◽  
Diagnostic Biomarkers ◽  
Cancer Diagnostic ◽  
Cross Platform

Nanomechanical sandwich assay for multiple cancer biomarkers in breast cancer cell-derived exosomes

Nanoscale ◽  
2016 ◽  
Vol 8 (33) ◽  
pp. 15137-15141 ◽  
Author(s):  
H. Etayash ◽  
A. R. McGee ◽  
K. Kaur ◽  
T. Thundat
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Biomarkers ◽  
Sandwich Assay ◽  
Multiple Cancer ◽  
Diagnostic Biomarkers ◽  
Cancer Diagnostic ◽  
Size Heterogeneity

The use of exosomes as cancer diagnostic biomarkers is technically limited by their size, heterogeneity and the need for extensive purification and labelling.

scholarly journals Small extracellular vesicle-encapsulated miR-181b-5p, miR-222-3p and let-7a-5p: Next generation plasma biopsy-based diagnostic biomarkers for inflammatory breast cancer

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250642
Author(s):  
Sarah Hamdy Ahmed ◽  
Nancy A. Espinoza-Sánchez ◽  
Ahmed El-Damen ◽  
Sarah Atef Fahim ◽  
Mohamed A. Badawy ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Progression ◽  
Inflammatory Breast Cancer ◽  
Roc Curves ◽  
Cost Effective ◽  
Extracellular Vesicle ◽  
Lymphatic Invasion ◽  
Precipitation Method ◽  
Liquid Biopsies ◽  
Diagnostic Biomarkers

Inflammatory breast cancer (IBC) is a rare, but aggressive entity of breast carcinoma with rapid dermal lymphatic invasion in young females. It is either poorly or misdiagnosed as mastitis because of the absence of a distinct lump. Small extracellular vesicles (sEVs) circulating in liquid biopsies are a novel class of minimally invasive diagnostic alternative to invasive tissue biopsies. They modulate cancer progression via shuttling their encapsulated cargo including microRNAs (miRNAs) into recipient cells to either trigger signaling or induce malignant transformation of targeted cells. Plasma sEVs < 200 nm were isolated using a modified cost-effective polyethylene glycol (PEG)-based precipitation method and compared to standard methods, namely ultracentrifugation and a commercial kit, where the successful isolation was verified by different approaches. We evaluated the expression levels of selected sEV-derived miR-181b-5p, miR-222-3p and let-7a-5p using quantitative real PCR (qPCR). Relative to non-IBC, our qPCR data showed that sEV-derived miR-181b-5p and miR-222-3p were significantly upregulated, whereas let-7a-5p was downregulated in IBC patients. Interestingly, receiver operating characteristic (ROC) curves analysis revealed that diagnostic accuracy of let-7a-5p alone was the highest for IBC with an area under curve (AUC) value of 0.9188, and when combined with miR-222-3p the AUC was improved to 0.973. Further, 38 hub genes were identified using bioinformatics analysis. Together, circulating sEV-derived miR-181b-5p, miR-222-3p and let-7a-5p serve as promising non-invasive diagnostic biomarkers for IBC.

scholarly journals Moving beyond local practice: Reconfiguring the adoption of a breast cancer diagnostic technology

2015 ◽  
Vol 131 ◽  
pp. 98-106 ◽  
Author(s):  
Gregory Maniatopoulos ◽  
Rob Procter ◽  
Sue Llewellyn ◽  
Gill Harvey ◽  
Alan Boyd
Keyword(s):  
Breast Cancer ◽  
Local Practice ◽  
Cancer Diagnostic ◽  
Diagnostic Technology

scholarly journals Clinical Validation of BCT Scores With Prognostic Factors in Hormone Receptor-positive, HER2-negative Early Breast Cancer

In Vivo ◽  
2019 ◽  
Vol 33 (6) ◽  
pp. 2133-2139
Author(s):  
JEEYEON LEE ◽  
WON HWA KIM ◽  
JIN HYANG JUNG ◽  
WAN WOOK KIM ◽  
CHAN SUB PARK ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prognostic Factors ◽  
Early Breast Cancer ◽  
Hormone Receptor ◽  
Clinical Validation ◽  
Hormone Receptor Positive

scholarly journals Non-Invasive Biomarkers for Early Detection of Breast Cancer

Cancers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2767
Author(s):  
Jiawei Li ◽  
Xin Guan ◽  
Zhimin Fan ◽  
Lai-Ming Ching ◽  
Yan Li ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Diagnosis ◽  
Early Stage ◽  
False Negative ◽  
Breast Cancer Diagnosis ◽  
Diagnostic Biomarkers ◽  
Circulating Micrornas ◽  
Non Invasive ◽  
Common Cancer ◽  
Volatile Organic

Breast cancer is the most common cancer in women worldwide. Accurate early diagnosis of breast cancer is critical in the management of the disease. Although mammogram screening has been widely used for breast cancer screening, high false-positive and false-negative rates and radiation from mammography have always been a concern. Over the last 20 years, the emergence of “omics” strategies has resulted in significant advances in the search for non-invasive biomarkers for breast cancer diagnosis at an early stage. Circulating carcinoma antigens, circulating tumor cells, circulating cell-free tumor nucleic acids (DNA or RNA), circulating microRNAs, and circulating extracellular vesicles in the peripheral blood, nipple aspirate fluid, sweat, urine, and tears, as well as volatile organic compounds in the breath, have emerged as potential non-invasive diagnostic biomarkers to supplement current clinical approaches to earlier detection of breast cancer. In this review, we summarize the current progress of research in these areas.

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