scholarly journals Glycine: a non-invasive imaging biomarker to aid magnetic resonance spectroscopy in the prediction of survival in paediatric brain tumours

Oncotarget ◽  
2018 ◽  
Vol 9 (27) ◽  
pp. 18858-18868 ◽  
Author(s):  
Ben Babourina-Brooks ◽  
Sarah Kohe ◽  
Simrandip K. Gill ◽  
Lesley MacPherson ◽  
Martin Wilson ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Brain Tumours ◽  
Imaging Biomarker ◽  
Resonance Spectroscopy ◽  
Non Invasive ◽  
Prediction Of Survival ◽  
Paediatric Brain Tumours

scholarly journals Magnetic resonance spectroscopy metabolite profiles predict survival in paediatric brain tumours

2013 ◽  
Vol 49 (2) ◽  
pp. 457-464 ◽  
Author(s):  
Martin Wilson ◽  
Carole L. Cummins ◽  
Lesley MacPherson ◽  
Yu Sun ◽  
Kal Natarajan ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Brain Tumours ◽  
Resonance Spectroscopy ◽  
Metabolite Profiles ◽  
Paediatric Brain Tumours

scholarly journals The development of a graphical user interface, functional elements and classifiers for the non-invasive characterization of childhood brain tumours using magnetic resonance spectroscopy

2011 ◽  
Vol 26 (3) ◽  
pp. 353-363 ◽  
Author(s):  
Alexander Gibb ◽  
John Easton ◽  
Nigel Davies ◽  
YU Sun ◽  
Lesley MacPherson ◽  
...  
Keyword(s):  
User Interface ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Brain Tumour ◽  
Brain Tumours ◽  
Resonance Spectroscopy ◽  
Non Invasive ◽  
Childhood Brain Tumours ◽  
Tumour Diagnosis ◽  
Brain Tumour Diagnosis

AbstractMagnetic resonance spectroscopy (MRS) is a non-invasive method, which can provide diagnostic information on children with brain tumours. The technique has not been widely used in clinical practice, partly because of the difficulty of developing robust classifiers from small patient numbers and the challenge of providing decision support systems (DSSs) acceptable to clinicians. This paper describes a participatory design approach in the development of an interactive clinical user interface, as part of a distributed DSS for the diagnosis and prognosis of brain tumours. In particular, we consider the clinical need and context of developing interactive elements for an interface that facilitates the classification of childhood brain tumours, for diagnostic purposes, as part of the HealthAgents European Union project. Previous MRS-based DSS tools have required little input from the clinician user and a raw spectrum is essentially processed to provide a diagnosis sometimes with an estimate of error. In childhood brain tumour diagnosis where there are small numbers of cases and a large number of potential diagnoses, this approach becomes intractable. The involvement of clinicians directly in the designing of the DSS for brain tumour diagnosis from MRS led to an alternative approach with the creation of a flexible DSS that, allows the clinician to input prior information to create the most relevant differential diagnosis for the DSS. This approach mirrors that which is currently taken by clinicians and removes many sources of potential error. The validity of this strategy was confirmed for a small cohort of children with cerebellar tumours by combining two diagnostic types, pilocytic astrocytomas (11 cases) and ependymomas (four cases) into a class of glial tumours which then had similar numbers to the other diagnostic type, medulloblastomas (18 cases). Principal component analysis followed by linear discriminant analysis on magnetic resonance spectral data gave a classification accuracy of 91% for a three-class classifier and 94% for a two-class classifier using a leave-one-out analysis. This DSS provides a flexible method for the clinician to use MRS for brain tumour diagnosis in children.

scholarly journals BIMG-08. DEUTERIUM MAGNETIC RESONANCE SPECTROSCOPY USING 2H-PYRUVATE ALLOWS NON-INVASIVE IN VIVO IMAGING OF TERT EXPRESSION IN BRAIN TUMORS

2021 ◽  
Vol 3 (Supplement_1) ◽  
pp. i2-i2
Author(s):  
Georgios Batsios ◽  
Celine Taglang ◽  
Meryssa Tran ◽  
Anne Marie Gillespie ◽  
Joseph Costello ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
In Vivo Imaging ◽  
Lactate Production ◽  
Telomere Maintenance ◽  
Resonance Spectroscopy ◽  
Low Grade ◽  
Non Invasive ◽  
Tert Expression

Abstract Telomere shortening constitutes a natural barrier to uncontrolled proliferation and all tumors must find a mechanism of maintaining telomere length. Most human tumors, including high-grade primary glioblastomas (GBMs) and low-grade oligodendrogliomas (LGOGs) achieve telomere maintenance via reactivation of the expression of telomerase reverse transcriptase (TERT), which is silenced in normal somatic cells. TERT expression is, therefore, a driver of tumor proliferation and, due to this essential role, TERT is also a therapeutic target. However, non-invasive methods of imaging TERT are lacking. The goal of this study was to identify magnetic resonance spectroscopy (MRS)-detectable metabolic biomarkers of TERT expression that will enable non-invasive visualization of tumor burden in LGOGs and GBMs. First, we silenced TERT expression by RNA interference in patient-derived LGOG (SF10417, BT88) and GBM (GS2) models. Our results linked TERT silencing to significant reductions in steady-state levels of NADH in all models. NADH is essential for the conversion of pyruvate to lactate, suggesting that measuring pyruvate flux to lactate could be useful for imaging TERT status. Recently, deuterium (2H)-MRS has emerged as a novel, clinically translatable method of monitoring metabolic fluxes in vivo. However, to date, studies have solely examined 2H-glucose and the use of [U-2H]pyruvate for non-invasive 2H-MRS has not been tested. Following intravenous injection of a bolus of [U-2H]pyruvate, lactate production was higher in mice bearing orthotopic LGOG (BT88 and SF10417) and GBM (GS2) tumor xenografts relative to tumor-free mice, suggesting that [U-2H]pyruvate has the potential to monitor TERT expression in vivo. In summary, our study, for the first time, shows the feasibility and utility of [U-2H]pyruvate for in vivo imaging. Importantly, since 2H-MRS can be implemented on clinical scanners, our results provide a novel, non-invasive method of integrating information regarding a fundamental cancer hallmark, i.e. TERT, into glioma patient management.

Hyperpolarized 129Xe Magnetic Resonance Spectroscopy in a Rat Model of Bronchopulmonary Dysplasia

2021 ◽  
Author(s):  
Jordan David Fliss ◽  
Brandon Zanette ◽  
Yonni Friedlander ◽  
Siddharth Sadanand ◽  
Andras A Lindenmaier ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Gas Exchange ◽  
Magnetic Resonance Spectroscopy ◽  
Bronchopulmonary Dysplasia ◽  
Rat Model ◽  
Resonance Spectroscopy ◽  
Gas Exchange Parameters ◽  
Functional Changes ◽  
Non Invasive

Premature infants often require mechanical ventilation and oxygen therapy which can result in bronchopulmonary dysplasia (BPD), characterized by developmental arrest and impaired lung function. Conventional clinical methods for assessing the prenatal lung are not adequate for the detection and assessment of long-term health risks in infants with BPD, highlighting the need for a non-invasive tool for the characterization of lung microstructure and function. Theoretical diffusion models, like the Model of Xenon Exchange (MOXE), interrogate alveolar gas exchange by predicting the uptake of inert Hyperpolarized (HP) 129Xe gas measured with HP 129Xe magnetic resonance spectroscopy (MRS). To investigate HP 129Xe MRS as a tool for non-invasive characterization of pulmonary microstructural and functional changes in vivo, HP 129Xe gas exchange data were acquired in an oxygen exposure rat model of BPD that recapitulates the fewer and larger distal airways and pulmonary vascular stunting characteristics of BPD. Gas exchange parameters from MOXE, including airspace mean chord length (L­m), apparent hematocrit in the pulmonary capillaries (HCT), and pulmonary capillary transit time (tx), were compared with airspace mean axis length and area density (MAL and ρ­A) and percentage area of tissue and air (PTA and PAA) from histology. L­m was significantly larger in the exposed rats (p=0.003) and correlated with MAL, ρ­A, PTA, and PAA (0.59<|ρ|<0.66 and p<0.05). Observed increase in HCT (p=0.012) and changes in tx are also discussed. These findings support the use of HP 129Xe MRS for detecting fewer, enlarged distal airways in this rat model of BPD, and potentially in humans.

scholarly journals The Privilege of Proton Magnetic Resonance Spectroscopy in Evaluating Ovarian Tumors

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
A A Hafez ◽  
M A Nasr ◽  
N L Salman
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Malignant Tumors ◽  
Radiology Department ◽  
Invasive Technique ◽  
Resonance Spectroscopy ◽  
Ovarian Mass ◽  
University Hospitals ◽  
Non Invasive

Abstract Background Exclusion of malignancy in ovarian mass is of paramount importance. It is the most crucial step after identification of a mass and it has a profound effect on the patient's management. So, a reliable method with which to differentiate a benign from a malignant ovarian mass would provide a basis for optimal preoperative planning and may also reduce the number of unnecessary laparotomies for patients undergoing treatment for benign disease. Objective The aim of our study is to highlight the role of magnetic resonance spectroscopy as a non-invasive technique which may effectively assist in differentiating benign from malignant ovarian masses. Patients and Methods This study included 20 patients with adnexal masses as suggested by preliminary pelvic ultrasound examination. referred from the Gynecology Department to the Radiology Department at Ain shams university hospitals. nine were benign, two were borderline, and six were malignant tumors and 3 were hemorrhagic cyst. Endometriosis, tubo-ovarian abscess. Results Our study revealed sharp choline peak in some benign as well as some malignant cases and so Cho peak could not help in the differential diagnosis between benign and malignant tumors, creatine, lipid and NAA were detected in both benign and malignant tumors, also Choline/Creatine Ratio fairly can differentiate between benign and malignant tumors with cut off point = 3.750 at sensitivity = 75.0% & specificity = 100.0% . Conclusion Our study had some factors that affect the results. First, the sample size were not enough to achieve a good results, second, diversity of samples and the complicated tumor histopathologic and morphologic features.

scholarly journals Prospective Longitudinal Analysis of 2-Hydroxyglutarate Magnetic Resonance Spectroscopy Identifies Broad Clinical Utility for the Management of Patients With IDH-Mutant Glioma

2016 ◽  
Vol 34 (33) ◽  
pp. 4030-4039 ◽  
Author(s):  
Changho Choi ◽  
Jack M. Raisanen ◽  
Sandeep K. Ganji ◽  
Song Zhang ◽  
Sarah S. McNeil ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Time Course ◽  
Imaging Biomarker ◽  
Resonance Spectroscopy ◽  
Lower Grade ◽  
Clinical Magnetic Resonance Imaging ◽  
Treatment Type ◽  
Longitudinal Imaging ◽  
Prospective Longitudinal

Purpose Proton magnetic resonance spectroscopy (MRS) of the brain can detect 2-hydroxyglutarate (2HG), the oncometabolite produced in neoplasms harboring a mutation in the gene coding for isocitrate dehydrogenase ( IDH). We conducted a prospective longitudinal imaging study to determine whether quantitative assessment of 2HG by MRS could serve as a noninvasive clinical imaging biomarker for IDH-mutated gliomas. Patients and Methods 2HG MRS was performed in 136 patients using point-resolved spectroscopy at 3 T in parallel with standard clinical magnetic resonance imaging and assessment. Data were analyzed in patient cohorts representing the major phases of the glioma clinical course and were further subgrouped by histology and treatment type to evaluate 2HG. Histologic correlations were performed. Results Quantitative 2HG MRS was technically and biologically reproducible. 2HG concentration > 1 mM could be reliably detected with high confidence. During the period of indolent disease, 2HG concentration varied by less than ± 1 mM, and it increased sharply with tumor progression. 2HG concentration was positively correlated with tumor cellularity and significantly differed between high- and lower-grade gliomas. In response to cytotoxic therapy, 2HG concentration decreased rapidly in 1p/19q codeleted oligodendrogliomas and with a slower time course in astrocytomas and mixed gliomas. The magnitude and time course of the decrease in 2HG concentration and magnitude of the decrease in tumor volume did not differ between oligodendrogliomas treated with temozolomide or carmustine. Criteria for 2HG MRS were established to make a presumptive molecular diagnosis of an IDH mutation in gliomas technically unable to undergo a surgical procedure. Conclusion 2HG concentration as measured by MRS was reproducible and reliably reflected the disease state. These data provide a basis for incorporating 2HG MRS into clinical management of IDH-mutated gliomas.

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