Association of IGF1R Polymorphisms with Growth Traits and its Expression Profiles in Different Pig Breeds

Insulin-like growth factor 1 receptor (IGF1R) is one component of insulin-like growth factor system, which has biological functions of growth traits. The aim of this study is to investigate the entire exons of IGF1R in the three commercial pig breeds, Duroc, Yorkshire and Landrace, to identify novel single nucleotide polymorphisms and their correlation with growth traits. One novel SNP (c.3678C>T) in the exon 20 was detected. This SNP caused the change of amino acid (Ser1217Phe), a portion of cytoplasmic tyrosine kinase domain of IGF1R. At the c.3678C>T site, three genotypes were significantly associated with average daily gain at different growth stage in Yorkshire and Landrace breeds. Meanwhile, we identified the differently expressed pattern of IGF1R in muscle of Yorkshire and Jinhua pigs at different growth stages. Our results provide useful information on understanding the effect of porcine IGF1R gene on growth. The novel IGF1R polymorphism may be useful as molecular markers in pig selection but future studies are required.

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Function of the Porcine TRPC1 Gene in Myogenesis and Muscle Growth

Cells ◽

10.3390/cells10010147 ◽

2021 ◽

Vol 10 (1) ◽

pp. 147

Author(s):

Yu Fu ◽

Peng Shang ◽

Bo Zhang ◽

Xiaolong Tian ◽

Ruixue Nie ◽

...

Keyword(s):

Transient Receptor Potential ◽

Growth Traits ◽

Expression Profiles ◽

Muscle Growth ◽

Receptor Potential ◽

Transient Receptor Potential Channel ◽

Nucleotide Polymorphisms ◽

Pig Breeds ◽

Muscle Tissues ◽

Transient Receptor

In animals, muscle growth is a quantitative trait controlled by multiple genes. Previously, we showed that the transient receptor potential channel 1 (TRPC1) gene was differentially expressed in muscle tissues between pig breeds with divergent growth traits base on RNA-seq. Here, we characterized TRPC1 expression profiles in different tissues and pig breeds and showed that TRPC1 was highly expressed in the muscle. We found two single nucleotide polymorphisms (SNPs) (C-1763T and C-1604T) in TRPC1 that could affect the promoter region activity and regulate pig growth rate. Functionally, we used RNAi and overexpression to illustrate that TRPC1 promotes myoblast proliferation, migration, differentiation, fusion, and muscle hypertrophy while inhibiting muscle degradation. These processes may be mediated by the activation of Wnt signaling pathways. Altogether, our results revealed that TRPC1 might promote muscle growth and development and plays a key role in Wnt-mediated myogenesis.

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The Role of the Tyrosine Kinase Domain of the Insulin-like Growth Factor-I Receptor in Intracellular Signaling, Cellular Proliferation, and Tumorigenesis

Journal of Biological Chemistry ◽

10.1074/jbc.270.49.29176 ◽

1995 ◽

Vol 270 (49) ◽

pp. 29176-29181 ◽

Cited By ~ 71

Author(s):

Catalina Hernández-Sánchez ◽

Vicky Blakesley ◽

Thea Kalebic ◽

Lee Helman ◽

Derek LeRoith

Keyword(s):

Growth Factor ◽

Tyrosine Kinase ◽

Intracellular Signaling ◽

Cellular Proliferation ◽

Kinase Domain ◽

Tyrosine Kinase Domain ◽

Insulin Like Growth Factor ◽

Factor I ◽

Growth Factor I

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Evaluation of Polymorphism in IGF-I and IGFB-3 Genes and their Relationship tith Twinning Rate and Growth Traits in Markhoz Goats

Annals of Animal Science ◽

10.1515/aoas-2016-0020 ◽

2017 ◽

Vol 17 (1) ◽

pp. 89-103 ◽

Cited By ~ 4

Author(s):

Sahar Rasouli ◽

Alireza Abdolmohammadi ◽

Alireza Zebarjadi ◽

Ali Mostafaei

Keyword(s):

Growth Factor ◽

Growth Traits ◽

Average Daily Gain ◽

Insulin Like Growth Factor ◽

Economic Traits ◽

Chi Square ◽

Genotype Frequencies ◽

Igf I ◽

I Gene ◽

Igfbp 3

AbstractGrowth rate and twinning rate are economic traits that can be used in goat breeding objectives. The aim of this study was to investigate polymorphisms in the insulin-like growth factor 1(IGF-I) and insulin-like growth factor binding protein (IGFBP-3) genes and their relationship with growth traits and twinning in Markhoz goats. Two sets of specific primers were used to amplify a 249bp fragment of IGF-I gene and a 316bp fragment of IGFBP-3 gene. PCR-SSCP analysis revealed three banding patterns for each gene that confirmed presence of a mutation in position 1617 of the IGF-I gene and a mutation in position 58 of IGFBP-3 gene. The genotype frequencies of IGF-I gene were 0.81 (GG), 0.16 (GA) and 0.03 (AA). Also, the genotype frequencies of IGFBP-3 gene were 0.79 (TT), 0.17 (TC) and 0.04 (CC). The Odds Ratio estimated for twinning rate was 1.11 for second on first parity, 0.19 for third on first parity and 5.71 for second on third parity. The chi-square statistics were 6.46 for IGF-I gene and 3.32 for IGFBP-3 gene. The results also indicated that different genotypes of these genes had no significant effect on birth weight, weight at 6 months, at 9 months and at 12 months but the interactions between different genotypes of IGF-I and IGFBP-3 genes were significant for weaning weight and average daily gain from birth to weaning. These results suggest that twinning rate in Markhoz breed is statistically affected by these genes and can be considered in breeding programs.

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Severe resistance to insulin and insulin-like growth factor-I in cells from a patient with leprechaunism as a result of two mutations in the tyrosine kinase domain of the insulin receptor

Metabolism ◽

10.1016/s0026-0495(96)90178-x ◽

1996 ◽

Vol 45 (12) ◽

pp. 1493-1500 ◽

Cited By ~ 23

Author(s):

Christèle Desbois-Mouthon ◽

Claude Danan ◽

Serge Amselem ◽

Marie-José Blivet-Van Eggelpoel ◽

Caroline Sert-Langeron ◽

...

Keyword(s):

Growth Factor ◽

Tyrosine Kinase ◽

Insulin Receptor ◽

Kinase Domain ◽

Tyrosine Kinase Domain ◽

Insulin Like Growth Factor ◽

Factor I ◽

Resistance To Insulin ◽

Growth Factor I ◽

In Cells

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Minireview: Nuclear Insulin and Insulin-like Growth Factor-1 Receptors: A Novel Paradigm in Signal Transduction

Endocrinology ◽

10.1210/en.2012-2165 ◽

2013 ◽

Vol 154 (5) ◽

pp. 1672-1679 ◽

Cited By ~ 54

Author(s):

Rive Sarfstein ◽

Haim Werner

Keyword(s):

Signal Transduction ◽

Transcription Factors ◽

Growth Factor ◽

Insulin Receptor ◽

Protein Interactions ◽

Kinase Domain ◽

Tyrosine Kinase Domain ◽

Insulin Like Growth Factor ◽

New Paradigm ◽

Surface Receptors

Abstract The specificity of the insulin receptor (InsR) and insulin-like growth factor-1 receptor (IGF1R) signaling pathways has been the focus of significant debate over the past few years. Recent evidence showing nuclear import and a direct transcriptional role for both InsR and IGF1R adds a new layer of complexity to this dialog. Hence, in addition to the classical roles associated with cell-surface receptors (eg, ligand binding, autophosphorylation of the tyrosine kinase domain, activation of insulin receptor substrate 1 (IRS-1) and additional substrates, protein-protein interactions with membrane and cytoplasm components), new data are consistent with nuclear (genomic) role(s) for both InsR and IGF1R. The present review provides a brief overview of the physical and functional similarities and differences between InsR and IGF1R and describes data from a number of laboratories providing evidence for a new layer of signaling regulation (ie, the ability of InsR and IGF1R to translocate to the cell nucleus and to elicit genomic activities usually associated with transcription factors). The ability of InsR and IGF1R to function as transcription factors, although poorly understood, constitutes a new paradigm in signal transduction. Although research on the role of nuclear InsR/IGF1R is still in its infancy, we believe that this rapidly developing area may have a major basic and translational impact on the fields of metabolism, diabetes, and cancer.

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