Concentration of Digestible Threonine in Diet on Production Performance and Intestinal Morphometry of WL Layers

2020 ◽  
Author(s):  
K. Naga Raja Kumari ◽  
P. Kalyani ◽  
S.V. Rama Rao ◽  
U. Rajkumar
Keyword(s):  
Barrier Function ◽  
Crude Protein ◽  
Egg Production ◽  
Production Performance ◽  
Mucosal Barrier ◽  
Treatment Groups ◽  
Mucin Gene ◽  
Mucosal Barrier Function ◽  
Muc2 Expression ◽  
Intestinal Morphometry

A study was conducted to evaluate the effect of digestible threonine in graded concentrations on production performance and mucosal barrier function in layers. A total of 180 layers at 25 weeks of age were randomly distributed into six treatment groups each with five replicates of 6 birds each. The experimental diets were isocaloric with three crude protein(CP) levels i.e. low 13.46% CP(LCP), medium 15.56% CP (MCP) and high 17.05% CP(HCP) and three lysine levels (13.46/0.65%; 15.56/0.60%; 17.00/0.70%) each with 63 and 66% threonine concentrations and were offered to treatment groups for 20 weeks. Increase (p less than 0.05) in egg production was observed with an increase in protein/lysine level and threonine concentration. Mucin gene (MUC2) expression was increased (p less than 0.05) in LCP group and was evident at 66% threonine concentrations in LCP and MCP and 63% threonine concentration in HCP. The results of the study concluded that the diet with 13.46% CP and 0.65% lysine at 66% threonine were optimum for layers at 25-44 weeks of age. 

scholarly journals Technical note: fluorescein as an indicator of enteric mucosal barrier function in preruminant lambs

2020 ◽  
Vol 98 (7) ◽  
Author(s):  
Audrey F Duff ◽  
Lisa R Bielke ◽  
Alejandro E Relling
Keyword(s):  
Oral Administration ◽  
Intestinal Permeability ◽  
Barrier Function ◽  
Animal Health ◽  
Fluorescein Isothiocyanate ◽  
Technical Note ◽  
Mucosal Barrier ◽  
Published Data ◽  
Treatment Groups ◽  
Mucosal Barrier Function

Abstract Increased intestinal permeability can be observed during the physiologic stress response and has been linked to suppression of animal health and performance. Previously published data have shown the efficacy of fluorescein isothiocyanate dextran (FITC-d; 4.17 mg/kg) as a marker of enteric inflammation and mucosal barrier function in multiple species. Fluorescein is a smaller, less expensive alternative molecule possessing similar properties. The following two experiments compared FITC-d and fluorescein as potential indicators of intestinal permeability in pre- and postweaned lambs administered daily intramuscular injections of dexamethasone (Dex; 0.1 mg/kg) for 1 wk. Experiment 1 consisted of five preweaned lambs that were placed in one of two treatment groups: fluorescein with Dex (F+Dex) or fluorescein only (F). On day 7, blood was collected before and 1 h after oral administration of fluorescein (50 mg/kg). Experiment 2 included 12 weaned lambs and four treatment groups: F+Dex, F, FITC-d with Dex (Fd+Dex), and FITC-d only (Fd). On day 7, blood was collected before and 2 h after oral administration of FITC-d (4.17 mg/kg) or fluorescein (50 mg/kg). Plasma fluorescence was reported as the ratio between T1h/T0 or T2h/T0 for experiment 1 or 2, respectively. Experiment 1 showed a significant increase in T1h/T0 ratio of F+Dex relative to F lambs (P = 0.05) indicative of increased leaky gut; however, no differences (P = 0.22) were obtained in experiment 2. Results of these experiments suggest fluorescein may serve as a suitable marker of enteric permeability in preruminant lambs, but not in those with functional rumens.

Glucagon-Like Peptide-2 Improve Intestinal Mucosal Barrier Function in Aged Rats

2018 ◽  
Vol 22 (6) ◽  
pp. 731-738 ◽  
Author(s):  
Weiying Ren ◽  
Jiayu Wu ◽  
Li Li ◽  
Y. Lu ◽  
Y. Shao ◽  
...  
Keyword(s):  
Barrier Function ◽  
Mucosal Barrier ◽  
Aged Rats ◽  
Intestinal Mucosal Barrier ◽  
Mucosal Barrier Function ◽  
Glucagon Like Peptide

scholarly journals Intestinal Mucosal Barrier Function Restoration in Mice by Maize Diet Containing Enriched Flavan-4-Ols

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 896 ◽  
Author(s):  
Binning Wu ◽  
Rohil Bhatnagar ◽  
Vijaya V. Indukuri ◽  
Shara Chopra ◽  
Kylie March ◽  
...  
Keyword(s):  
Barrier Function ◽  
Control Diet ◽  
Intestinal Barrier ◽  
Mucosal Barrier ◽  
Intestinal Barrier Function ◽  
Low Grade ◽  
Colonic Inflammation ◽  
Mucosal Barrier Function ◽  
Inflammatory Bowel ◽  
Histology Score

Inflammatory bowel disease (IBD), a chronic intestinal inflammatory condition, awaits safe and effective preventive strategies. Naturally occurring flavonoid compounds are promising therapeutic candidates against IBD due to their great antioxidant potential and ability to reduce inflammation and improve immune signaling mediators in the gut. In this study, we utilized two maize near-isogenic lines flavan-4-ols-containing P1-rr (F+) and flavan-4-ols-lacking p1-ww (F−) to investigate the anti-inflammatory property of flavan-4-ols against carboxymethylcellulose (CMC)-induced low-grade colonic inflammation. C57BL/6 mice were exposed to either 1% CMC (w/v) or water for a total of 15 weeks. After week six, mice on CMC treatment were divided into four groups. One group continued on the control diet. The second and third groups were supplemented with F+ at 15% or 25% (w/w). The fourth group received diet supplemented with F− at 15%. Here we report that mice consuming F+(15) and F+(25) alleviated CMC-induced increase in epididymal fat-pad, colon histology score, pro-inflammatory cytokine interleukin 6 expression and intestinal permeability compared to mice fed with control diet and F−(15). F+(15) and F+(25) significantly enhanced mucus thickness in CMC exposed mice (p < 0.05). These data collectively demonstrated the protective effect of flavan-4-ol against colonic inflammation by restoring intestinal barrier function and provide a rationale to breed for flavan-4-ols enriched cultivars for better dietary benefits.

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