First Report on Better Functional Property of Black Chicken Meat from India

2021 ◽  
Author(s):  
Renuka Sehrawat ◽  
Rekha Sharma ◽  
Sonika Ahlawat ◽  
Vivek Sharma ◽  
Mohan Singh Thakur ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Breast Meat ◽  
Vitro Assay ◽  
Antioxidant Power ◽  
Meat Extract ◽  
Scavenging Capacity

Background: Indian domesticated chickens have a wide variety of phenotypes. Unlike other chicken breeds, Kadaknath chicken has black meat and is used by indigenous tribal people for its invigorating and therapeutic properties. To look for the functional traits that might be contributing towards the acclaimed benefits, free radical scavenging capacity and metal chelating ability of Kadaknath meat were explored for the first time in comparison to the commercial Cobb broiler. Methods: During the period 2018-2020, breast and thigh meat were collected from chickens (n=20/ group) at their commercial slaughter age. Meat extract was used for qualitative evaluation of protein as well as the antioxidant capacity utilizing diverse in vitro methods corresponding to different antioxidation mechanisms.Result: Protein concentration (g/100g of tissue) in Kadaknath breast (25.25 ± 0.31) and thigh (19.98 ± 0.29) meat was significantly (P less than 0.05) higher than the Cobb. Breast meat had better scavenging capacity than the thigh. The superiority of the antioxidant capacity of Kadaknath meat was explicitly established by more than one in vitro assay. Free radical scavenging assays viz. 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS); 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Cupric reducing antioxidant capacity (CUPRAC) had significantly (P less than 0.05) higher values for Kadaknath in comparison to the Cobb broiler meat. Ferric reducing antioxidant power (FRAP) values (mM Fe2+/g of tissue) were also more in breast meat of Kadaknath (26.97±0.37) than the Cobb (15.24±0.40). Similarly, FRAP values were higher in Kadaknath (33.85±0.47) than the Cobb (19.2±0.31) thigh meat. Kadaknath had higher antioxidant capacity as reflected by metal chelation inhibition value. These findings help to explain the unique nutritional and functional characteristics of Kadaknath black-bone chicken and provide basic research data for exploring the commercial potential of its meat in the fields of functional foods, cosmeceuticals and nuutraceuticals.

Effect of 1-MCP on Bioavailability of Lingwu Long Jujube In Vitro Digestion

2012 ◽  
Vol 554-556 ◽  
pp. 1357-1360
Author(s):  
Zhong Li Jiang ◽  
Ai Li Wang ◽  
Xi Hong Li ◽  
Min Peng Zhu ◽  
Jun Wei Wang
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Control Samples

The present study investigated the effect of 1-MCP on bioavailability of Lingwu long jujube stored at 0 °C for 60 days. At the end of the storage, compared with the control samples, Lingwu long jujube treated with 1-MCP exhibited higher Vc bioaccessibility, total phenolic content and free radical scavenging capacity, which demonstrated that 1-MCP treatment could provide a better effect on maintaining the bioavailability of Lingwu long jujube.

scholarly journals Effects of Fruit Maturity on Physicochemical Properties, Sugar Accumulation and Antioxidant Capacity of Wild Harvested Kakadu Plum (Terminalia ferdinandiana)

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 48
Author(s):  
Anh Dao Thi Phan ◽  
Maral Seidi Damyeh ◽  
Saleha Akter ◽  
Mridusmita Chaliha ◽  
Michael E. Netzel ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Soluble Sugars ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Maturity Stages ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Kakadu Plum ◽  
Terminalia Ferdinandiana

Terminalia ferdinandiana (Kakadu plum), belonging to the family Combretaceae, is endemic to Australia and has a long history of traditional medicinal applications and food cuisine by the Australian Indigenous people. This study investigated the effects of maturity stages on the morphology, physicochemical parameters (total soluble solids (TSS), total acid content (TAC), and pH), soluble sugar profile and antioxidant capacity of Kakadu plum (KP) fruits that were wild harvested from different trees and classified into four different maturity stages (immature to mature). TSS and TAC were determined by standard assays/procedures, main sugars by UHPLC–MS/MS and antioxidant capacity (total phenolic content (TPC) and DPPH free radical scavenging capacity) by spectrophotometry. The results showed that soluble sugars (glucose, sucrose and fructose) ranging from 1.3 to 17.7% dry weight (DW), TSS (17.0–52.7% DW) and TAC (1.3–6.7% DW) increased with maturity. However, antioxidant capacity (TPC in the range of 7.4–21.9% DW and DPPH free radical scavenging capacity from 22 to 76% inhibition at the extract concentration of 20 g·L−1) did not follow the same trend as the one observed for soluble sugars, TSS and TAC. These differences were associated with the tree-to-tree variability as a consequence of the wild harvest condition. This study provides important information to both the KP industry and Indigenous enterprises regarding the selection of the appropriate maturity stage to harvest KP fruit to target for different markets (e.g., low-sugar vs. high-sugar fruit).

Quality Characteristics of Breast and Thigh Chicken Meat from Free- Range System: Comparative Antioxidant Profile of Indigenous and Improved Germplasm

2020 ◽  
Author(s):  
Renuka Sehrawat ◽  
Rekha Sharma ◽  
Sonika Ahlawat ◽  
Vivek Sharma ◽  
M.S. Thakur ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Environmental Sustainability ◽  
Radical Scavenging ◽  
Qualitative Evaluation ◽  
Chicken Meat ◽  
Poultry Production ◽  
Antioxidant Power ◽  
Meat Extract ◽  
Metal Chelating

Background: New chicken breeds are being evolved for backyard rural poultry production to overcome the slow growth, late sexual maturity and poor production of indigenous breeds. However, autochthonous poultry is epitomized for quality attributes of their products. With this in mind, the present study for the first time explored the antioxidant capacity of meat obtained from a unique Indian chicken, Kadaknath and a synthetic breed of poultry, Jabalpur colour (JBC). Methods: During the period 2018-2020, breast and thigh meat were collected from chickens (n=20/ group) at their commercial slaughter age (20 weeks). Meat extract was used for qualitative evaluation. Antioxidant activity was explored using five well established in vitro methods testing for different antioxidant mechanisms. Result: Both, Kadaknath and JBC meat was proteinaceous with higher protein concentration (g/100 g of wet weight) in the breast (Kadaknath, 25.21±0.31 and JBC, 25.65±0.39) than the thigh (Kadaknath, 19.98±0.29 and JBC, 19.04±0.23). Both the groups exhibited antioxidant capacity in all the assays. They showed good radical scavenging for ABTS and DPPH free radicals. Superiority of Kadaknath meat was ascertained unequivocally by the three assays viz. Ferric reducing antioxidant power (FRAP), lipid oxidation inhibition (TBARS) and metal chelating capacity. FRAP values (mM Fe2+/g of tissue) were 26.97±0.37 and 33.85±0.47 (Kadaknath) and 22.84±0.25 and 26.82±0.36 (JBC) for breast and thigh, respectively. Similarly, Kadaknath meat was more potent (% inhibition) iron chelator (breast, 62.71±0.99 and thigh, 75.07±0.98) in comparison to the JBC (breast, 46.30±2.36 and thigh, 63.12±1.87). Breast meat had better scavenging capacity than the thigh except in FRAP and metal chelating assays. Results provide insight into the antioxidant potential of backyard poultry germplasm thus, laying foundation for developing marketing strategies targeting consumers interested in nutritional quality, animal welfare and environmental sustainability. Furthermore, baseline data has been generated for studying medicinal properties attributed to the black chicken meat of Kadaknath.

Antioxidant and free radical scavenging potential of crude sulphated polysaccharides from Turbinaria ornata

Biologia ◽  
2015 ◽  
Vol 70 (1) ◽  
Author(s):  
M. Manoj Saravana Guru ◽  
Mani Vasanthi ◽  
Anant Achary
Keyword(s):  
Free Radical ◽  
Superoxide Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Antioxidant Power ◽  
Sulphated Polysaccharides ◽  
Turbinaria Ornata ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Total Antioxidant

AbstractThe sulphated polysaccharides from brown algae have been attracting extensive interest due to their numerous biological activities. The present study was designed to evaluate the antioxidant activity and free-radical scavenging capacity of crude sulphated polysaccharides (CSP) from Turbinaria ornata, a marine brown algae. The CSP was extracted from T. ornata using hot water with the yield of 14.31% (w/w). The chemical composition analysis of CSP showed 71.07 ± 4.61 % of total sugar, 1.81 ± 0.035 % total protein, 27 ± 1.49 % sulphate, and 6.16 ± 0.36 % total phenol. The presence of sulphated polysaccharides in the CSP was confirmed by agarose gel electrophoresis. The in vitro antioxidant activity of CSP was evaluated using total antioxidant power assay and ferric reducing antioxidant power assay (FRAP). The results revealed that the total antioxidant capacity of CSP was 22.205 ± 0.875 (equivalent mg of ascorbic acid/g of CSP) and 0.556 ± 0.03 (mM of FeSO4/mg of CSP) FRAP value, respectively. The free-radical scavenging ability of CSP was demonstrated using 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and superoxide radical. The capacity of the CSP to scavenge 50% of free radicals was found to be 88.71 ± 1.01, 440.07 ± 4.43 and 352 ± 4.58 μg/mL for ABTS, DPPH and superoxide radical, respectively. These results showed that the CSP from T. ornata possess antioxidant and free-radical scavenging capacity.

scholarly journals Solvent polarity mediates phytochemical yield and antioxidant capacity of Isatis tinctoria

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7857
Author(s):  
Abdul Wakeel ◽  
Sohail Ahmad Jan ◽  
Ikram Ullah ◽  
Zabta Khan Shinwari ◽  
Ming Xu
Keyword(s):  
Free Radical ◽  
Secondary Metabolites ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Solvent Polarity ◽  
Free Radical Scavenging ◽  
Isatis Tinctoria ◽  
Polarity Index ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

Secondary metabolites have been extensively used in the treatment of various health problems. The role of solvent polarity on the phytochemical isolation and antioxidant capacity of Isatis tinctoria (woad) is elusive. In the present study, 14 solvents with different polarity were used in the extraction and total phenolic and flavonoid content (TPC and TFC) investigation. Ferricyanide, phosphomolybdenum, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods were used to calculate and compare the antioxidant/free radical scavenging capacity. Our results showed that solvent polarity greatly affects TPC and TFC yield, which is mainly increasing with increasing solvent polarity index and suddenly decreasing at very high polarity. The comparative results showed that TPC is directly correlated with reducing power, antioxidant, and free radical scavenging capacity. Taken together, we conclude that different woad plant parts contain different level of secondary metabolites with a specific polarity that requires a particular solvent with an appropriate polarity index for the extraction. The identification of these biologically active crude extracts and fractions are very important for the basic biological sciences, pharmaceutical applications, and future research for HPLC based active compounds isolation.

scholarly journals Chemopreventative Potential of Plant-Based Extracts Coffee (Coffea) and Mauby (Colubrina Arborescens) using the Global Antioxidant Response (GAR) Method via in vitro Simulated Digestion

2021 ◽  
Vol 10 (3) ◽  
pp. 43
Author(s):  
Hannah Gross ◽  
Cheryl Rock ◽  
Yada Treesukosol ◽  
Virginia Gray
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Response ◽  
Free Radical Scavenging ◽  
Antioxidant Power ◽  
Simulated Digestion ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

Plant-based extracts such as coffee (coffea) and Mauby (Colubrina Arborescens) were tested for chemopreventative potential by measuring their antioxidant activity (i.e., reducing power and free radical scavenging capacity) conventionally using chemical assays 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP). Alternately, extracts were also analyzed for their chemopreventative potential via a novel method (Global Antioxidant Response [GAR]), where they were subjected to simulated digestion before their antioxidant activity was determined via conventional methods previously mentioned. Next, the antioxidant capacities of the extracts, conventional versus the novel (GAR) method were compared. Overall, the analysis indicated that the reducing power (FRAP) and free radical scavenging capacity (DPPH) of coffee and Mauby were reduced (~16%) after undergoing simulated digestion. It was also observed that while the antioxidants in Mauby scavenged radicals at a significantly higher capacity than those in coffee (95.7% ± 0.67 and 90% ± 2.1 before digestion, and 77.7% ± 2.2 and 74.6% ± 2.3 after digestion, respectively), antioxidants in coffee exhibited higher reducing power compared to those in Mauby. Specifically, after undergoing simulated digestion, 1.12 mM FeSO4/mL ± 0.05 to 0.68 mM FeSO4/mL ± 0.07 ions were reduced before digestion, and 0.73 mM FeSO4/mL ± 0.09 to 0.48 mM FeSO4/mL ± 0.04 ions were reduced after digestion for coffee and Mauby, respectively. These findings suggest that while the antioxidants in coffee may have been more powerful in their ability to reduce ions, the antioxidants in Mauby may have been more effective in scavenging and neutralizing radicals.

Synthesis, Characterization, Free-radical Scavenging Capacity and Antioxidant Activity of Novel Series of Hydrazone, 1,3,4-oxadiazole and 1,2,4- triazole Derived from 3,5-dimethyl-1H-pyrazole

2019 ◽  
Vol 16 (7) ◽  
pp. 712-720 ◽  
Author(s):  
Khalid Karrouchi ◽  
Saad Fettach ◽  
Smaail Radi ◽  
El bekkaye Yousfi ◽  
Jamal Taoufik ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Cancer ◽  
Ic50 Values

Background: Pyrazole is an important class of heterocyclic compound, has been shown to exhibit diverse biological and pharmacological activities such as anti-inflammatory, anti-cancer, antioxidant, etc. Methods: In this study, a series of novel 3,5-dimethyl-1H-pyrazole derivatives containing hydrazine 4a-l have been synthesized via the reaction of the 2-(3,5-dimethyl-1H-pyrazol-1-yl)acetohydrazide. All synthesized compounds have been tested for their in vitro antioxidant activities via utilization of 1,1-biphenyl-2-picrylhydrazyl (DPPH) as a free radical scavenging reagent. Results: The data reported herein indicates that compound 4k showed potential radical scavenging capacity and compounds 4f and 4g exhibited best activity for the iron binding while comparing with positive controls. Conclusion: Good activity was noted for some compounds. In particular, compound 4k showed the highest antioxidant activity with IC50 values of 22.79 ± 3.64 and 1.35 ± 0.66 μg/mL in the DPPH and ABTS tests, respectively.

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