scholarly journals ​Identification of Suitable Reference Genes for qPCR Analysis of 4T1 Mouse Mammary Tumor Cell Line

2021 ◽  
Author(s):  
Richa Arora ◽  
Waseem Akram Malla ◽  
Arpit Tyagi ◽  
Shikha Saxena ◽  
Sonalika Mahajan ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Mammary Tumor ◽  
Reference Genes ◽  
Tumor Cell Line ◽  
Mammary Tumor Cell ◽  
Mammary Tumor Cell Line ◽  
Mouse Mammary Tumor ◽  
Mouse Mammary Tumor Cell ◽  
Suitable Reference

Background: Identification of candidate reference genes for real time PCR study is a preliminary requirement to normalize experimental data and thus, deduce a reliable conclusion. Complex tissues like mouse mammary gland constitutes various cell types which makes it difficult to identify reference gene constantly expressing under different experimental conditions. Methods: In this study we have identified suitable reference genes for 4T1 tumor cell line derived from mouse mammary tumor cells. We have studied four genes namely Gapdh, Actb, Prdx1 and Ctbp1 for their expression stability in CPV2.NS1 post transfected 4T1 cells by Best Keeper. Result: By our study, three reference genes i.e. Prdx1, Gapdh and Ctbp1 were found to be quite correlated with the BestKeeper index, but by considering all three criteria of selection by BestKeeper algorithm, Prdx1 showed minimum standard deviation and coefficient of variation and was found to be ranked at first position by BestKeeper which suggests Prdx1 to be considered as better internal control gene among all other reference genes taken in our study for qPCR based experiments in 4T1 mouse mammary tumor cell line transfected with CPV2.NS1

Docetaxel alone or orally combined with 5-fluorouracil and its derivatives: effects on mouse mammary tumor cell line MM2 in vitro and in vivo

2001 ◽  
Vol 12 (8) ◽  
pp. 691-698 ◽  
Author(s):  
Yasutaka Takeda ◽  
Iwao Yoshizaki ◽  
Yasumasa Nonaka ◽  
Hironobu Yanagie ◽  
Akio Matsuzawa ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Mammary Tumor ◽  
Tumor Cell Line ◽  
Mammary Tumor Cell ◽  
Mammary Tumor Cell Line ◽  
Mouse Mammary Tumor ◽  
Mouse Mammary Tumor Cell

scholarly journals Establishment and Characterization of a Brca1−/−, p53−/− Mouse Mammary Tumor Cell Line

2020 ◽  
Vol 21 (4) ◽  
pp. 1185
Author(s):  
Lilla Hámori ◽  
Gyöngyi Kudlik ◽  
Kornélia Szebényi ◽  
Nóra Kucsma ◽  
Bálint Szeder ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Cell ◽  
Mammary Tumor ◽  
Triple Negative ◽  
Tumor Cell Line ◽  
Breast Cancers ◽  
Mammary Tumor Cell ◽  
Mammary Tumor Cell Line ◽  
Mouse Mammary Tumor ◽  
Mouse Mammary Tumor Cell

Breast cancer is the most commonly occurring cancer in women and the second most common cancer overall. By the age of 80, the estimated risk for breast cancer for women with germline BRCA1 or BRCA2 mutations is around 80%. Genetically engineered BRCA1-deficient mouse models offer a unique opportunity to study the pathogenesis and therapy of triple negative breast cancer. Here we present a newly established Brca1−/−, p53−/− mouse mammary tumor cell line, designated as CST. CST shows prominent features of BRCA1-mutated triple-negative breast cancers including increased motility, high proliferation rate, genome instability and sensitivity to platinum chemotherapy and PARP inhibitors (olaparib, veliparib, rucaparib and talazoparib). Genomic instability of CST cells was confirmed by whole genome sequencing, which also revealed the presence of COSMIC (Catalogue of Somatic Mutations in Cancer) mutation signatures 3 and 8 associated with homologous recombination (HR) deficiency. In vitro sensitivity of CST cells was tested against 11 chemotherapy agents. Tumors derived from orthotopically injected CST-mCherry cells in FVB-GFP mice showed sensitivity to cisplatin, providing a new model to study the cooperation of BRCA1-KO, mCherry-positive tumor cells and the GFP-expressing stromal compartment in therapy resistance and metastasis formation. In summary, we have established CST cells as a new model recapitulating major characteristics of BRCA1-negative breast cancers.

scholarly journals Copper nanoparticles stabilized with cashew gum: Antimicrobial activity and cytotoxicity against 4T1 mouse mammary tumor cell line

2019 ◽  
Vol 34 (2) ◽  
pp. 188-197 ◽  
Author(s):  
Adriany Amorim ◽  
Ana Carolina Mafud ◽  
Silvania Nogueira ◽  
Joilson Ramos- Jesus ◽  
Alyne Rodrigues de Araújo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Tumor Cell ◽  
Copper Nanoparticles ◽  
Mammary Tumor ◽  
Tumor Cell Line ◽  
Mammary Tumor Cell ◽  
Mammary Tumor Cell Line ◽  
Cashew Gum ◽  
Mouse Mammary Tumor ◽  
Mouse Mammary Tumor Cell
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