Prediction of Field Performance of Seed Lots of Groundnut (Arachis hypogaea L.) through Vigour Tests

2021 ◽  
Author(s):  
N.K. Biradarpatil ◽  
Smayli Rana ◽  
Shivasharanappa S. Patil
Keyword(s):  
Electrical Conductivity ◽  
Field Performance ◽  
Plant Population ◽  
Accelerated Ageing ◽  
Natural Ecosystem ◽  
Seed Vigour ◽  
Germination Time ◽  
Pod Yield ◽  
Mean Germination Time ◽  
Highly Correlated

Background: Profitable farming indeed needs a seed which emerges and performs better under natural ecosystem. The inadequacy of seed germination test in predicting the field performance of a seed lot, as it is carried out under controlled laboratory conditions which may not meet in the field necessitates the development and standardization of tests in a way that would reflect the performance of seeds in the field through laboratory tests.Methods: A laboratory based seed vigour tests were undertaken with 20 different seed lots of groundnut variety GPBD-4 having different vigour levels to predict the field performance. The correlation and regression analysis among the laboratory based seed vigour tests and field performance was analyzed.Conclusion: The significance of lab vigour test on the field performance revealed that, electrical conductivity was highly negatively correlated to seed emergence, speed of emergence, plant population and pod yield with r = - 0.885, - 0.885, -0.843 and -0.845, respectively. Similarly, accelerated ageing was highly correlated to plant population (r = 0.864), plant height (0.737) and pod yield (r = 0.815). Whereas, the mean germination time calculated based on radicle emergence test contributed more than 80% to field performances and mean while, electrical conductivity influencing for 71.4% variation in the pod yield. Thus, hinting refinement and use of electric conductance, accelerated ageing and mean germination time tests towards efficient prediction of field performance of groundnut seed lots.

scholarly journals Seed vigour evaluation in petunia seed lots to predict seedling emergence and longevity

2020 ◽  
Vol 48 (3) ◽  
pp. 391-400
Author(s):  
Ibrahim Demir ◽  
Nihal Erturk ◽  
Zeynep Gokdas
Keyword(s):  
Electrical Conductivity ◽  
Seedling Emergence ◽  
Accelerated Ageing ◽  
Seed Vigour ◽  
Germination Time ◽  
Ageing Test ◽  
Radicle Emergence ◽  
Mean Germination Time ◽  
Storage Potential ◽  
And Storage

This study examined the potential of different vigour tests to reveal differences in performance of nine hybrid petunia seed lots. Saturated salt accelerated ageing test (SSAA) and 48-hour radicle emergence (RE48h) tests were able to predict seedling emergence and longevity potential. The results of accelerated ageing (AA) (at 41/43°C, 48/72 hours, 100% RH), saturated salt accelerated ageing (SSAA) (at 41/43/45°C, 72 hours, 75% RH), mean germination time (MGT), 48-hour radicle emergence (RE48h) and electrical conductivity (EC) tests were compared with seedling emergence in modules and longevity (at 5°C for 24 months and 25°C for 12 months). Initial standard germination (SG) percentages were above 83% in the lots. Seedling emergence ranged between 18 and 89%, germination after storage was between 16 and 88% at 5°C, and 13 and 96% at 25°C. The SSAA test at 43°C and 45°C for 72 hours was correlated with both seedling emergence and longevity. RE48h was correlated with emergence and germination after storage at 5°C for 24 months; MGT and SG was correlated with seedling emergence. Neither AA nor the EC test was correlated with emergence and longevity. Thus vigour, expressed as both emergence and storage potential, was successfully predicted by the SSAA test and RE48h.

scholarly journals Potential of a single radicle emergence count in predicting field emergence of Desi chickpea seed lots as an alternative vigour test

2019 ◽  
Author(s):  
Maliheh Akbarpour ◽  
Mohammad Khajeh-Hosseini ◽  
Alireza Seifi
Keyword(s):  
Electrical Conductivity ◽  
Coefficient Of Determination ◽  
Time Intervals ◽  
Crop Species ◽  
Seed Vigour ◽  
Germination Time ◽  
Regular Time ◽  
Radicle Emergence ◽  
Mean Germination Time ◽  
Field Emergence

Single early counts of radicle emergence (RE) is a validated vigour test for four crop species. We performed this study to see whether an early radicle emergence (RE) count predicts the emergence of seedlings in Desi chickpea seed lots as well as an electrical conductivity (EC) test. Electrical conductivity measurements of 11 seed lots were highly predictive of field emergence (FE) (R2 = 0.626**). The radicle emergence was counted at regular time intervals of six hours, up to 72 hours after imbibition. Mean germination time (MGT) clearly predicted variation in number of seedlings in the field. This evidence supports the idea that the variation in the germination time between seed lots can be an early indicator of seed vigour. Early RE counts at 48 hours also had a high coefficient of determination (R2 = 0.764***) with FE. The negative correlation between EC and the single early count of RE at different germination times indicated that the two tests identified the same differences in vigour. The strong relationships between RE at 48 hours with both EC and FE supports the proposal that a single count of RE could be an alternative vigour test for Desi chickpea.

scholarly journals Evaluation of Seed Quality Attributes of Sorghum Germplasm Accessions from Eastern, Coastal and Nyanza Regions, Kenya

2020 ◽  
Vol 9 (3) ◽  
pp. 9
Author(s):  
C. W. Muui ◽  
R. M. Muasya ◽  
S. Nguluu ◽  
A. Kambura
Keyword(s):  
Food Security ◽  
Seed Quality ◽  
Germination Percentage ◽  
Seed Vigour ◽  
Subsistence Farmers ◽  
Germination Time ◽  
Vigour Index ◽  
Mean Germination Time ◽  
Handling Practices ◽  
Germplasm Accessions

Sorghum (Sorghum bicolor L.) is an important cereal crop in Kenya. Despite the crops importance, the yields attained by farmers in Eastern, Coastal and Nyanza regions of Kenya remain low. Access to good quality seeds of sorghum is one of the constraints facing the subsistence farmers. Good quality seed is important for increasing yield to attain food security. The aim of this study was to evaluate quality attributes of the seed used by farmers. A total of 108 germplasm accessions were obtained from 76 farmers. The seeds were tested for time and percentage of germination, seed vigour index, shoot and root dry weight. Data collected was subjected to analysis of variance. Means were separated using Fisher’s Least Significance Difference test at p ≤ 0.05. Seed samples of 26 accessions attained germination percentage below stipulated standards by Seeds and Plant Varieties Act CAP 326. Majority of seeds showed longer mean germination time with only nine accessions germinating in less than ten days. Seed vigour index was relatively high in most of the accessions, while biomass accumulation varied from high to very low among accessions. Though most of the seeds attained a high germination percentage, about 92% of seeds showed longer mean germination time. The environmental conditions in the fields, pre and post harvest handling practices impact on the seed quality hence the wide variability in germination percentage, germination time, seed vigour index and dry matter accumulation. Therefore the need to improve quality of seeds used by subsistence farmers by providing extension services on best pre and post harvest handling practices. Increasing production of sorghum in these regions will contribute significantly towards realizing food security. Further analysis could be carried out on genetic and sanitary quality aspects of the seeds planted by farmers in Eastern, Nyanza and Coastal regions.

scholarly journals Seed Vigour Tests to Estimate Seedling Emergence in Cress (Lepidium sativum L.) Seed Lots

2019 ◽  
Vol 47 (3) ◽  
Author(s):  
Ibrahim DEMIR ◽  
Burcu B. KENANOGLU ◽  
Eren ÖZDEN
Keyword(s):  
Electrical Conductivity ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Lepidium Sativum ◽  
Accelerated Ageing ◽  
Digital Object Identifier ◽  
Seed Vigour ◽  
Significance Levels ◽  
Radicle Emergence ◽  
Lepidium Sativum L

This work was carried out to estimate field and controlled room seedling emergence potential through seed vigour tests in cress (Lepidium sativum L.) seeds. Early radicle emergence percentages after 12 (RE12h), 24 (RE24h) and 36 (RE36h) hours of germination test, mean germination time, accelerated aging (45 °C, 100% RH, 24 h), electrical conductivity (EC) of soaking water (40 ml, 50 seeds, 20 °C), after 16 hours (EC16h) and 24 hours (EC24h), and EC16h and EC24h after accelerated ageing (AA, 45 °C, 100% RH, 24h) were tested as vigour tests in ten commercial seed lots of cress. Standard laboratory germination ranged between 88 and 93%. Seeds were sown on two occasions in field and controlled room conditions, and seedling emergence percentages were determined after 30 days in the soil and 14 days in the controlled room. Seedling emergence ranged between 67 and 85% and 59 and 83% in the first and second sowings in the field. These values were 75 and 92% in controlled room sowing. Vigour tests were correlated to seedling emergence potential at various significance levels but RE24h and EC16h showed the highest correlation coefficient values (p < 0.001) in all three sowing conditions as r = 0.879-0.988 in RE24h, and r = 0.902-0.962 in EC16h. Results indicated that early radicle emergence percentages after 24 hours (RE24h) and electrical conductivity value after 16 hours (EC16h) can be successfully used to estimate the seedling emergence potential of cress seeds in field and controlled room conditions.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

scholarly journals Effect of winter wheat (cv. Jana) seed ageing on its viability and vigour

2014 ◽  
Vol 52 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Eugeniusz Sójka ◽  
Riad Zaidan
Keyword(s):  
Oxidative Phosphorylation ◽  
Winter Wheat ◽  
Respiratory Activity ◽  
Germination Test ◽  
Seed Vigour ◽  
Germination Time ◽  
Seed Ageing ◽  
Mean Germination Time ◽  
Biogenesis Of Mitochondria ◽  
The Mean

Viability and vigour of freshly collected 1, 2 and 3 years old seeds of the winter wheat cv. Jana were determined, on the base of the rate of germination, germinative capacity and the mean germination time, as well as the length of the shoot after 4, 7 and 10 days of the germination test. The intensity of respiration of whole caryopses, of isolated embryos and of the mitochondria was also measured. It was shown that the seed vigour and viability decreases with ageing. In seeds stored over longer periods the respiratory activity decreases as consequence of disturbance of the biogenesis of mitochondria, decline of oxidative phosphorylation and a decrease of the ability to oxidize the respiratory substrate.

scholarly journals An assessment of the physiological quality of sorghum (Sorghum bicolor L Moench) seeds planted by farmers in Bomet District of Kenya

2012 ◽  
Vol 12 (53) ◽  
pp. 6385-6396
Author(s):  
LA Ochieng ◽  
◽  
PW Mathenge ◽  
R Muasya ◽  
Keyword(s):  
Electrical Conductivity ◽  
Sorghum Bicolor ◽  
Germination Time ◽  
The People ◽  
High Germination ◽  
Quality Aspects ◽  
Physiological Quality ◽  
Mean Germination Time ◽  
Sorghum Bicolor L

Sorghum ( Sorghum bicolor L. Moench) is an important crop that enhances food security in a region . As a food crop, sorghum is nutritious , containing starch (75- 79%) as the major component, followed by protein (6.0 -16.1%) and oil (2.1- 5.0%). Despite the crop’s versatility, it is regrettable that sorghum yields are still fairly low in Bomet District of Kenya. One of the constraints associated with low yields is the accessibility to good quality seeds by farmers . It was assumed in this study that use of low quality seed was among the factors that could be significantly affecting the low production of sorghum in the district. This study focused on the physiological quality of seed which refers to the germination capacity, viability, characteristics related to dormancy and vigour of the seed. A total of 100 farmers were interviewed and 80 seed sample s were obtained from 80 farmers. The seeds were subjected to viability and vigour quality tests. These tests included germination (as a viability test); mean germination time and electrical conductivity tests (as vigour tests). About 29% of the seed samples taken for germination test were below the laboratory standards as stipulated in the seeds and Plant Varieties Act (CAP 326). Critical electrical conductivity values and critical mean germination time values were derived during the study . In accordance with the critical values arrived at in this study, 27.5% of the seed samples showed relatively high electric al conductivity readings and 36.3% of the seed samples showed longer mean germination time. However, 11.3% of the seed samples showed high germination percentages of above 90%. Since about 29% of farmers seeds were of bad physiological quality, it was concluded that this proportion of seed can result in poor yields and hence cause food insecurity to the people of the society , and thus require s further consideration for improvement . This study recommends that further research be conducted on the genetic, physical and sanitary quality aspects of seed planted by farmers in Bomet District so that a definite conclusive statement about the quality of seed planted by farmers in the District can be made.

scholarly journals Electrical conductivity and mineral composition of the imbibition solution of bean seeds during storage

2012 ◽  
Vol 36 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Claudia Denise da Silva ◽  
Mariana Silva Rosa Pazeto ◽  
Roberval Daiton Vieira
Keyword(s):  
Electrical Conductivity ◽  
Mineral Composition ◽  
Storage Period ◽  
Seed Storage ◽  
Effect Of Temperature ◽  
Accelerated Ageing ◽  
Bean Seed ◽  
Seed Vigour ◽  
Calcium Magnesium ◽  
And Storage

The electrical conductivity test has been used to evaluate seed vigour in many species, especially legumes, such as peas, beans and soybeans. The objective of this research was to evaluate the effect of temperature and storage period on the results of the electrical conductivity test and the mineral composition of the imbibition solution of bean seeds (Phaseolus vulgaris L.). Three seed lots of the cultivar IAPAR - 81 and cultivar IPR - TIZIU were stored at 10, 25 and 25-10º C (six months at 10º C and six months at 25º C) The following evaluations were made every three months for a year: seed water content, germination and vigour (accelerated ageing and electrical conductivity) and the potassium, calcium and magnesium contents of the imbibition solution. The cultivars showed similar behavior in storage. The reduction in the vigour of bean seeds stored at 10º C was verified by accelerated ageing and electrical conductivity tests. The bean seed storage at low temperature (10º C) does not influence the results of the evaluation of seed vigour by electrical conductivity test and the quantification of calcium, magnesium and potassium ions. The evaluation of vigour by the electrical conductivity test is not recommended for bean seed stored by long periods (above 9 months).

An analysis of the differences in germination of seed lots of perennial ryegrass in response to artificial ageing

1989 ◽  
Vol 112 (3) ◽  
pp. 351-357 ◽  
Author(s):  
R. E. L. Naylor
Keyword(s):  
Normal Distribution ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Accelerated Ageing ◽  
Artificial Ageing ◽  
Germination Time ◽  
Before And After ◽  
Mean Germination Time ◽  
The Mean ◽  
Viable Seeds

SummaryStatistically sound methods for analysing the results of germination tests were used to quantify and assess differences between seed lots of perennial ryegrass (Lolium perenne L.) subjected to accelerated ageing. Ageing altered not only the proportion of seed not germinating but also the mean germination time and the synchrony of germination. Seed lots differed in these three parameters before and after ageing. The responses to ageing of mean germination time and synchrony were not regular and a simulation of trie possible effects of ageing on germination suggested this irregularity might be due to changes in the proportion of viable seeds and to a non-normal distribution of seed ages.

scholarly journals Single count of radicle emergence, DNA replication during seed germination and vigour in alfalfa seed lots

2020 ◽  
Vol 48 (3) ◽  
pp. 367-380
Author(s):  
M. Cheshmi ◽  
M. Khajeh-Hosseini
Keyword(s):  
Dna Replication ◽  
Close Association ◽  
Field Performance ◽  
Root Tip ◽  
Strongly Correlated ◽  
Seed Vigour ◽  
Germination Time ◽  
Alfalfa Seed ◽  
Radicle Emergence ◽  
Alfalfa Seeds

Previous work on alfalfa seeds has illustrated the close association between the rate of germination and vigour. This study examined single early counts of radicle emergence (RE) as an indirect method to measure the rate of germination in alfalfa, and investigated cell cycle activities in the early stages of imbibition. The 6-hour interval counts of just germination (JG) and germination percentages (G) of 14 seed lots at 20, 17, 15 and 13°C were compared with final emergence and mean emergence time. Single counts of JG and G were strongly correlated with mean just germination time (MJGT), mean germination time (MGT) (P < 0.01) and field performance. The results indicate that single counts of RE after 24 hours at 20°C could be used to predic the vigour of alfalfa seeds. The embryo root tip cells of six seed lots with low, medium and high vigour were used for analysis of DNA replication by flow cytometry 3, 6, 12, 18 and 24 hours after imbibition. The 4C/2C ratio 18 hours after imbibition and the increase in 4C/2C ratio between 6 and 12 hours after imbibition was associated with the vigour of the seed lot and has the potential to predict seed vigour.

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