scholarly journals Seed Vigour Tests to Estimate Seedling Emergence in Cress (Lepidium sativum L.) Seed Lots

2019 ◽  
Vol 47 (3) ◽  
Author(s):  
Ibrahim DEMIR ◽  
Burcu B. KENANOGLU ◽  
Eren ÖZDEN
Keyword(s):  
Electrical Conductivity ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Lepidium Sativum ◽  
Accelerated Ageing ◽  
Digital Object Identifier ◽  
Seed Vigour ◽  
Significance Levels ◽  
Radicle Emergence ◽  
Lepidium Sativum L

This work was carried out to estimate field and controlled room seedling emergence potential through seed vigour tests in cress (Lepidium sativum L.) seeds. Early radicle emergence percentages after 12 (RE12h), 24 (RE24h) and 36 (RE36h) hours of germination test, mean germination time, accelerated aging (45 °C, 100% RH, 24 h), electrical conductivity (EC) of soaking water (40 ml, 50 seeds, 20 °C), after 16 hours (EC16h) and 24 hours (EC24h), and EC16h and EC24h after accelerated ageing (AA, 45 °C, 100% RH, 24h) were tested as vigour tests in ten commercial seed lots of cress. Standard laboratory germination ranged between 88 and 93%. Seeds were sown on two occasions in field and controlled room conditions, and seedling emergence percentages were determined after 30 days in the soil and 14 days in the controlled room. Seedling emergence ranged between 67 and 85% and 59 and 83% in the first and second sowings in the field. These values were 75 and 92% in controlled room sowing. Vigour tests were correlated to seedling emergence potential at various significance levels but RE24h and EC16h showed the highest correlation coefficient values (p < 0.001) in all three sowing conditions as r = 0.879-0.988 in RE24h, and r = 0.902-0.962 in EC16h. Results indicated that early radicle emergence percentages after 24 hours (RE24h) and electrical conductivity value after 16 hours (EC16h) can be successfully used to estimate the seedling emergence potential of cress seeds in field and controlled room conditions.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

scholarly journals Seed vigour evaluation in petunia seed lots to predict seedling emergence and longevity

2020 ◽  
Vol 48 (3) ◽  
pp. 391-400
Author(s):  
Ibrahim Demir ◽  
Nihal Erturk ◽  
Zeynep Gokdas
Keyword(s):  
Electrical Conductivity ◽  
Seedling Emergence ◽  
Accelerated Ageing ◽  
Seed Vigour ◽  
Germination Time ◽  
Ageing Test ◽  
Radicle Emergence ◽  
Mean Germination Time ◽  
Storage Potential ◽  
And Storage

This study examined the potential of different vigour tests to reveal differences in performance of nine hybrid petunia seed lots. Saturated salt accelerated ageing test (SSAA) and 48-hour radicle emergence (RE48h) tests were able to predict seedling emergence and longevity potential. The results of accelerated ageing (AA) (at 41/43°C, 48/72 hours, 100% RH), saturated salt accelerated ageing (SSAA) (at 41/43/45°C, 72 hours, 75% RH), mean germination time (MGT), 48-hour radicle emergence (RE48h) and electrical conductivity (EC) tests were compared with seedling emergence in modules and longevity (at 5°C for 24 months and 25°C for 12 months). Initial standard germination (SG) percentages were above 83% in the lots. Seedling emergence ranged between 18 and 89%, germination after storage was between 16 and 88% at 5°C, and 13 and 96% at 25°C. The SSAA test at 43°C and 45°C for 72 hours was correlated with both seedling emergence and longevity. RE48h was correlated with emergence and germination after storage at 5°C for 24 months; MGT and SG was correlated with seedling emergence. Neither AA nor the EC test was correlated with emergence and longevity. Thus vigour, expressed as both emergence and storage potential, was successfully predicted by the SSAA test and RE48h.

scholarly journals Electrical conductivity of the seed soaking solution and soybean seedling emergence

2004 ◽  
Vol 61 (2) ◽  
pp. 164-168 ◽  
Author(s):  
Roberval Daiton Vieira ◽  
Angelo Scappa Neto ◽  
Sonia Regina Mudrovitsch de Bittencourt ◽  
Maristela Panobianco
Keyword(s):  
Electrical Conductivity ◽  
Water Content ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Soybean Seedling ◽  
Seed Soaking ◽  
Laboratory Conditions ◽  
Field And Laboratory Conditions

Vigor of soybean [Glycine max (L.) Merrill] seeds can be evaluated by measuring the electrical conductivity (EC) of the seed soaking solution, which has shown a satisfactory relationship with field seedling emergence, but has not had aproper definition of range yet. This work studies the relationship between EC and soybean seedling emergence both in the field and laboratory conditions, using twenty two seed lots. Seed water content, standard germination and vigor (EC, accelerated aging and cold tests) were evaluated under laboratory conditions using -0.03; -0.20; -0.40 and -0.60 MPa matric potentials, and field seedling emergence was also observed. There was direct relationship between EC and field seedling emergence (FE). Under laboratory conditions, a decreasing relationship was found between EC and FE as water content in the substrate decreased. Relationships between these two parameters were also found when -0.03; -0.20 and -0.40 MPa matric potentials were used. EC tests can be used successfully to evaluate soybean seed vigor and identify lots with higher or lower field emergence potential.

scholarly journals Assessment of physiological potential of stored pea (Pisum sativum L.) seeds

2013 ◽  
Vol 35 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Fábio Mielezrski ◽  
Julio Marcos-Filho
Keyword(s):  
Electrical Conductivity ◽  
Growth Habit ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Storage ◽  
Seed Vigor ◽  
Seedling Length ◽  
Pea Seeds ◽  
Cold Chamber ◽  
Pea Seed

Research on pea seed storage and the identification of efficient seed vigor tests for this species is still insufficient, especially for cultivars that produce wrinkled seeds used for consumption in natura. The objective of this study was to verify the accuracy of tests to assess the physiological potential of pea seed lots stored in different environments. Four seed lots of two cultivars, 'Telefone Alta' (indeterminate growth habit) and 'Itapuã' (determinate growth habit) were stored under different environmental conditions for 8 months: a) laboratory uncontrolled b) dry and cold chamber (10 ºC and 30% RH), c) controlled environment (20 ºC and 70% RH). Seed vigor (accelerated aging, electrical conductivity, seedling length and seedling emergence) was evaluated initially and after four and eight months storage. Results showed that the use of seed lots of high initial physiological potential is fundamental for guaranteeing the desired performance of pea seeds during storage. Wrinkled pea seeds should be stored between harvest and sowing in an environment in which the sum of the relative humidity (%) and temperature (°C) does not exceed 70. Physiological potential of pea seeds should be evaluated by at least two vigor tests; consideration to accelerated aging and electrical conductivity tests is recommended.

scholarly journals Physiological potential of eggplant seeds

2013 ◽  
Vol 35 (2) ◽  
pp. 225-230 ◽  
Author(s):  
Magnólia de Mendonça Lopes ◽  
Clíssia Barboza da Silva ◽  
Roberval Daiton Vieira
Keyword(s):  
Electrical Conductivity ◽  
Seed Germination ◽  
Moisture Content ◽  
Seedling Emergence ◽  
Solanum Melongena ◽  
The Other ◽  
Accelerated Ageing ◽  
Preliminary Information ◽  
H 41 ◽  
Controlled Deterioration

The use and efficiency of vigor tests depend on the plant species and the development and, or adjustment of methodology, to obtain efficient procedures for evaluating seed physiological potential. The objective of this study was to compare different methods for evaluating the physiological potential of eggplant seeds (Solanum melongena L). Six seed lots of the "Comprida Roxa" cultivar were submitted to the following tests: germination, first count germination, seedling emergence, controlled deterioration (18, 21 and 24% for 24 and 48 hours at 45 °C), accelerated ageing (AA) and saturated salt accelerated ageing (both at 41 and 45 °C for 24, 48 and 72 hours) and electrical conductivity (25 seeds, 50 and 75 mL of water for 1, 2, 4, 8, 12, 16 and 24 hours of imbibition at 25 °C). The first count test provided preliminary information on seed germination and the other tests, in general, gave similar results for seedling emergence. It was concluded that the physiological potential of eggplant seeds can be evaluated using AA for 48 h/41 °C and controlled deterioration at 24% moisture content/ 24 h/45 °C. The best conditions for the electrical conductivity test on eggplant seed are using 25 seeds in 50mL of water for four hours at 25 ºC.

scholarly journals ELECTRICAL CONDUCTIVITY AND ETHANOL RELEASE TO ASSESS RED RICE SEED VIGOR1

2021 ◽  
Vol 34 (4) ◽  
pp. 791-798
Author(s):  
RAFAEL MARANI BARBOSA ◽  
MATHEUS ANDRÉ DE JESUS ◽  
RAFAELA ALVES PEREIRA ◽  
GEDEON ALMEIDA GOMES JUNIOR
Keyword(s):  
Electrical Conductivity ◽  
Efficient Method ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Seed Sample ◽  
Red Rice ◽  
Germination Test ◽  
Rice Seeds

ABSTRACT To evaluate seed vigor, electrical conductivity and ethanol tests are fast and efficient methodologies. They have the potential to be used in several species, such as red rice. However, there are no protocols or information about their efficiency. Thus, the objective was to evaluate the efficiency, and define parameters of execution for electrical conductivity and ethanol tests, to evaluate the vigor of red rice seeds. The study was conducted using four lots of ‘BRS 901’ red rice, which was subjected to a germination test, as well as first count, accelerated aging, and field seedling emergence tests. The electrical conductivity test was performed with 25, 50, and 100 seeds soaked in 50 mL and 75 mL of water, at 25 °C and 30 °C, for 3, 6, 20, and 24 hours, respectively. The ethanol test was performed with 50 and 100 seeds soaked in a volume of water equivalent to 1.0, 1.5, 2.0, 2.5, and 3.0× the mass of the seed sample. To assess the vigor of red rice seeds, the electrical conductivity test is an efficient method when conducted with 50 seeds soaked in 50 mL of water at 25 °C for 20 hours. Meanwhile, the ethanol test is most effective when performed with 50 seeds, in a volume of water that is 2.5× the mass of the sample, at 40 °C for 24 hours.

scholarly journals Seedling image analysis and traditional tests to evaluate onion seed vigor

2017 ◽  
Vol 39 (3) ◽  
pp. 216-223 ◽  
Author(s):  
Nayara Roberto Gonçalves ◽  
Silvio Moure Cicero ◽  
Haynna Fernandes Abud
Keyword(s):  
Electrical Conductivity ◽  
Image Analysis ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Seedling Length ◽  
Onion Seed ◽  
Emergence Percentage

Abstract: The aim of this study was to verify the possibility of using computerized seedling image analysis to detect differences in vigor among onion seed lots and to compare this analysis with traditional vigor tests. We used five seed lots of two onion cultivars, Bella Catarina and Bella Vista. The Seed Vigor Imaging System (SVIS®) software was used to process the seedling images and assess seedling length and vigor through seedling uniformity and growth indexes. These determinations were compared to traditional tests used to evaluate onion seed vigor: germination percentage and first germination count, seedling emergence percentage and emergence speed, accelerated aging, and electrical conductivity. Results showed that vigor differences among onion seed lots can be identified using the SVIS® in a manner similar to traditional vigor tests; assessments should be performed at five days after sowing.

Assessment of soybean seed vigour tests for eastern Australia

1996 ◽  
Vol 36 (1) ◽  
pp. 99
Author(s):  
M Boersma ◽  
M Law ◽  
SW Adkins
Keyword(s):  
Incubation Period ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Field Capacity ◽  
Seed Vigour ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Eastern Australia ◽  
Controlled Deterioration

Vigour testing was undertaken on seed from 4 soybean [Glycine max (L.) Merr.] cultivars taken from field trials in eastern Australia. The seed from each cultivar exhibited moderate to good germination and a range in vigour. Vigour tests consisted of the controlled deterioration and modified versions of the accelerated aging test. Accelerated aging was carried out in 4 ways using 2 periods of incubation (76 and 96 h), with or without the seed protectant Thiram to control saprophytic fungi. Results were compared with seedling emergence from the same seed samples when planted in a controlled environment glasshouse. A combination of 2 day/night temperatures, cold (18/13�C), warm (33/28�C), and 3 soil moisture regimes, dry (-0.3 MPa), field capacity (-0.01 MPa) and saturated soil, produced a total of 6 different environments. Emergence was also recorded under a moderate daylnight temperature of 23/18�C in soil at field capacity. The standard germination test and modified accelerated aging test with a 72-h incubation period with Thiram were significantly (P<0.01) correlated with emergence from all 7 soil environments. A 96-h period of accelerated aging (with or without Thiram) tended to suppress germination and there was no correlation with emergence from most soil environments. The controlled deterioration test correlated with seedling emergence from most soil environments except for dry soil. The vigour test which gave the best indicator of performance under a range of different seedbed moisture and temperature conditions was the modified accelerated aging test using a 72-h incubation period with Thiram.

scholarly journals Computerized imaging analysis of seedlings for assessment of physiological potential of wheat seeds

2012 ◽  
Vol 34 (4) ◽  
pp. 589-596 ◽  
Author(s):  
Vanessa Neumann Silva ◽  
Francisco Guilhien Gomes Junior ◽  
Silvio Moure Cicero
Keyword(s):  
Electrical Conductivity ◽  
Image Analysis ◽  
Relative Humidity ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Wheat Cultivars ◽  
Imaging Analysis ◽  
Wheat Seeds

Nowadays, image analysis is one of the most modern tools in evaluating physiological potential of seeds. This study aimed at verifying the efficiency of the seedling imaging analysis to assess physiological potential of wheat seeds. The seeds of wheat, cultivars IAC 370 and IAC 380, each of which represented by five different lots, were stored during four months under natural environmental conditions of temperature (T) and relative humidity (RH), in municipality of Piracicaba, Stated of São Paulo, Brazil. For this, bimonthly assessments were performed to quantify moisture content and physiological potential of seeds by means of tests of: germination, first count, accelerated aging, electrical conductivity, seedling emergence, and computerized analysis of seedlings, using the Seed Vigor Imaging System (SVIS®). It has been concluded that the computerized analyses of seedling through growth indexes and vigor, using the SVIS®, is efficient to assess physiological potential of wheat seeds.

Prediction of Field Performance of Seed Lots of Groundnut (Arachis hypogaea L.) through Vigour Tests

2021 ◽  
Author(s):  
N.K. Biradarpatil ◽  
Smayli Rana ◽  
Shivasharanappa S. Patil
Keyword(s):  
Electrical Conductivity ◽  
Field Performance ◽  
Plant Population ◽  
Accelerated Ageing ◽  
Natural Ecosystem ◽  
Seed Vigour ◽  
Germination Time ◽  
Pod Yield ◽  
Mean Germination Time ◽  
Highly Correlated

Background: Profitable farming indeed needs a seed which emerges and performs better under natural ecosystem. The inadequacy of seed germination test in predicting the field performance of a seed lot, as it is carried out under controlled laboratory conditions which may not meet in the field necessitates the development and standardization of tests in a way that would reflect the performance of seeds in the field through laboratory tests.Methods: A laboratory based seed vigour tests were undertaken with 20 different seed lots of groundnut variety GPBD-4 having different vigour levels to predict the field performance. The correlation and regression analysis among the laboratory based seed vigour tests and field performance was analyzed.Conclusion: The significance of lab vigour test on the field performance revealed that, electrical conductivity was highly negatively correlated to seed emergence, speed of emergence, plant population and pod yield with r = - 0.885, - 0.885, -0.843 and -0.845, respectively. Similarly, accelerated ageing was highly correlated to plant population (r = 0.864), plant height (0.737) and pod yield (r = 0.815). Whereas, the mean germination time calculated based on radicle emergence test contributed more than 80% to field performances and mean while, electrical conductivity influencing for 71.4% variation in the pod yield. Thus, hinting refinement and use of electric conductance, accelerated ageing and mean germination time tests towards efficient prediction of field performance of groundnut seed lots.

scholarly journals Electrical conductivity and soybean seedling emergence

2004 ◽  
Vol 61 (4) ◽  
pp. 386-391 ◽  
Author(s):  
Julio Cesar Fachini Colete ◽  
Roberval Daiton Vieira ◽  
Alek Sandro Dutra
Keyword(s):  
Electrical Conductivity ◽  
Water Potential ◽  
Laboratory Tests ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Before And After ◽  
Glycine Max L ◽  
The Relationship

Evaluation of soybean [Glycine max (L.) Merrill] seeds vigor and its relation with field seedling emergence can provide secure indicatives of seed physiological potential. The present research studied the relationship between the electrical conductivity test and the seedling emergence of soybean in field and laboratory. The field experiment was sown in November 26th, December 3td and 10th, 2000. Laboratory tests were performed using levels of water potential in the substrate (soil) -0.03; -0.2; -0.4; and -0.6 MPa. Fourteen seed lots, seven of 'Embrapa 48' and seven of 'BRS 133' soybean cultivars were used. Laboratory tests comprised seed water content determination, before and after the accelerated aging; germination and vigor tests (accelerated aging and electrical conductivity). Germination and seedling emergence decreased as the substrate water potential was reduced, indicating a relationship among the germination, water potential and seed vigor. For the 'Embrapa 48' higher vigor seed lots, the best performance occurred at -0.6 MPa water potential. The electrical conductivity test may be efficient to evaluate soybean seed vigor and, consequently, the performance potential in the field. However, further studies are necessary to determine the values or the range of values that indicate the seed vigor level and the adequate use of a given seed lot.

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