Genome Analysis of Mungbean [Vigna radiata (L.) Wilczek] using Simple Sequence Repeats (SSR) Mapping Data

2021 ◽  
Author(s):  
Babita Rani ◽  
Renu Singh ◽  
Minakshi Jattan ◽  
Shubham Kumar ◽  
Ram Kumar
Keyword(s):  
Genetic Diversity ◽  
Agricultural Research ◽  
Ammonium Bromide ◽  
Breeding Lines ◽  
Iron And Zinc ◽  
Zinc Concentrations ◽  
Increase Productivity ◽  
Cetyltrimethyl Ammonium ◽  
Simple Sequence

Background: Agricultural research chiefly focuses on the ways to increase productivity of staple food crops like wheat and rice, but still there are crops where research focus is meagre like nutritionally important mungbean crop grown by marginal farmers in crop rotation systems. Mungbean is leguminous crop which is high in protein content thus it offers health benefits at cheaper rates. The present work emphasizes on finding genetic diversity in mungbean germplasm on the basis of chemical and molecular analysis for micronutrients variation (iron and zinc). The identified molecular markers having linkage with high iron and zinc concentrations in the seeds can prove helpful in expansion of biofortification programme.Methods: Fifty-one green gram genotypes viz. varieties released from CCS Haryana Agricultural University (HAU), Hisar, Punjab Agricultural University (PAU), Ludhiana, Indian Institute of Pulse Research (IIPR), Kanpur and some advanced breeding lines were included in the study. Acid digested samples were used for determination of Fe and Zn by Atomic Absorption Spectrophotometer. Young seedlings leaves were used for isolation of genomic DNA using 2% CTAB (cetyltrimethyl ammonium bromide). Result: Total of fifty-one mungbean genotypes were tested using fifty simple sequence repeat (SSR) primers. Out of fifty primers screened, 16 primers generated 35 bands. Iron (Fe) and zinc (Zn) in mungbean lines was 36.90 to 107.1 mg/kg and 14.2 to 53.8 mg/kg respectively. The molecular studies based on SSR markers also indicates existence of ample genetic diversity at molecular level.

Inter Simple Sequence Repeat (ISSR) markers to assess genetic diversity and relatedness within strawberry genotypes

2008 ◽  
Vol 88 (2) ◽  
pp. 313-322 ◽  
Author(s):  
S. C. Debnath ◽  
S. Khanizadeh ◽  
A. R. Jamieson ◽  
C. Kempler
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Genetic Similarity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Group Method ◽  
Sequence Repeat ◽  
Breeding Lines ◽  
Pair Group ◽  
Simple Sequence

The goal of this study was to determine the level of genetic diversity and relatedness among 16 strawberry (Fragaria H ananassa Duch.) cultivars and 11 breeding lines developed in Canada, using Inter Simple Sequence Repeat (ISSR) markers. Seventeen primers generated 225 polymorphic ISSR-PCR bands. Cluster analysis by the unweighted pair-group method with arithmetic averages (UPGMA) revealed a substantial degree of genetic similarity among the genotypes ranging from 63 to 77% that were in agreement with the principal coordinate (PCO) analysis. Geographical distribution for the place of breeding program explained only 1.4% of total variation as revealed by analysis of molecular variance (AMOVA). The ISSR markers detected a sufficient degree of polymorphism to differentiate among strawberry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in current strawberry breeding programs. Key words: Fragaria × ananassa, DNA fingerprinting, multivariate analysis, breeding, genetic similarity

Development of a Dispersive Liquid–Liquid Microextraction Method Combined with UV-Visible Spectrophotometry for Determination of Trace Aluminum(III) in Water, Wastewater, Food, Biological, and Pharmaceutical Samples

2017 ◽  
Vol 100 (1) ◽  
pp. 224-229 ◽  
Author(s):  
Nasrin Taghipour Birgani ◽  
Shahla Elhami
Keyword(s):  
Dynamic Range ◽  
Sample Volume ◽  
Ammonium Bromide ◽  
Cetyltrimethyl Ammonium Bromide ◽  
Reagent Blank ◽  
Uv Visible ◽  
Cetyltrimethyl Ammonium ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

Abstract A simple and sensitive method was proposed for the preconcentration of trace levels of Al(III) prior to its determination by spectrophotometry, based on dispersive liquid–liquid microextraction. The complexation of the Al(III) was performed by chelation with Eriochrome Cyanine R (ECR). In this method, cetyltrimethyl ammonium bromide (CTAB) as a dispersant was dissolved in chloroform as an extractant solvent, and then the solution was rapidly injected by a syringe into the samples containing Al(III), which had already been complexed by ECR at optimized pH. Various parameters were studied and optimized for a 10 mL sample volume. Under the optimum conditions, the LOD (3 times the SD of 10 replicate readings of the reagent blank) and the dynamic range of the calibration obtained were 0.2 ng mL−1 (7 nM) and 1.0–80.0 ng mL−1, respectively. The RSDs for eight replicate determinations of10 and 60 ng mL−1 of Al(III) were 3.3 and 1.8%, respectively. This strategy was successfully applied to determine the Al concentration in water, wastewater,yogurt, apple, carrot, celery, bread, potato, urine, and Al–Mg syrup samples.

scholarly journals Molecular Identification Keys for Cultivars and Lines of Cornus florida and C. kousa Based on Simple Sequence Repeat Loci

2008 ◽  
Vol 133 (6) ◽  
pp. 783-793 ◽  
Author(s):  
Phillip A. Wadl ◽  
Xinwang Wang ◽  
Andrew N. Trigiano ◽  
John A. Skinner ◽  
Mark T. Windham ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Identification ◽  
Simple Sequence Repeat ◽  
Floral Display ◽  
Cornus Florida ◽  
Sequence Repeat ◽  
Breeding Lines ◽  
Flowering Dogwood ◽  
Identification Keys ◽  
Simple Sequence

Flowering dogwood (Cornus florida) and kousa dogwood (C. kousa) are popular ornamental species commonly used in the horticultural industry. Both trees are valued for their beautiful floral display and four-season appeal. Species-specific simple sequence repeat (SSR) loci were used to genotype and assess genetic diversity of 24 flowering dogwood cultivars and breeding lines and 22 kousa dogwood cultivars. Genetic diversity was determined by allele sharing distances and principal coordinate analysis and was high in both species. Molecular identification keys were developed for cultivars and breeding lines of each species using a few polymorphic SSRs loci (four in C. florida and five in C. kousa). Most (18 of 24) of the flowering dogwood and all (22 of 22) kousa dogwood accessions could be distinguished from each other using these SSRs; those that could not were resolved using DNA amplification fingerprinting. The reliability of both keys was assessed using five anonymous cultivars for each dogwood species, which were correctly identified using the molecular keys. The genetic information presented here will be useful for identification and verification of cultivars for nurseries and as molecular markers for breeders and researchers.

Effect of Cetyltrimethyl Ammonium Bromide on Electrochemical Determination of Dexamethasone

2010 ◽  
Vol 22 (20) ◽  
pp. 2330-2338 ◽  
Author(s):  
Rajendra N. Goyal ◽  
Sanghamitra Chatterjee ◽  
Anoop Raj Singh Rana
Keyword(s):  
Electrochemical Determination ◽  
Ammonium Bromide ◽  
Cetyltrimethyl Ammonium Bromide ◽  
Cetyltrimethyl Ammonium

MWCNT–CTAB modified glassy carbon electrode as a sensor for the determination of paracetamol

RSC Advances ◽  
2015 ◽  
Vol 5 (61) ◽  
pp. 49045-49053 ◽  
Author(s):  
Jayant I. Gowda ◽  
Danavva G. Gunjiganvi ◽  
Nagaveni B. Sunagar ◽  
Manjushree N. Bhat ◽  
Sharanappa T. Nandibewoor
Keyword(s):  
Carbon Nanotube ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Ammonium Bromide ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
Cetyltrimethyl Ammonium Bromide ◽  
Multiwalled Carbon ◽  
Cetyltrimethyl Ammonium

An electrochemical sensor for the sensitive detection of paracetamol (PCM) was developed by constructing a glassy carbon electrode (GCE) modified with multiwalled carbon nanotube–cetyltrimethyl ammonium bromide (MWCNT–CTAB).

scholarly journals Development of a composite collection for mining germplasm possessing allelic variation for beneficial traits in chickpea

2006 ◽  
Vol 4 (1) ◽  
pp. 13-19 ◽  
Author(s):  
H.D. Upadhyaya ◽  
B.J. Furman ◽  
S.L. Dwivedi ◽  
S.M. Udupa ◽  
C.L.L. Gowda ◽  
...  
Keyword(s):  
Agricultural Research ◽  
Allelic Variation ◽  
Reference Sample ◽  
Grain Legume ◽  
Breeding Lines ◽  
Germplasm Collections ◽  
Breeding Populations ◽  
Beneficial Traits ◽  
Elite Breeding Lines ◽  
Simple Sequence

Chickpea is one of the most important grain legume crops in the world. Large collections of genetic resources are maintained in the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) and International Center for Agricultural Research in the Dry Areas (ICARDA) genebanks. Association mapping using neutral markers has been suggested as a means to identify useful alleles in the vast reservoirs of genetic diversity existing in the germplasm collections that could be associated with the phenotypes among the population individuals. ICRISAT in collaboration with ICARDA developed a global composite collection of 3000 accessions that will be profiled using 50 polymorphic simple sequence repeat (SSR) markers. The data generated through this collaborative effort will be used to define the genetic structure of the global composite collection and to select a reference sample of 300 accessions representing the maximum diversity for the isolation of allelic variants of candidate gene associated with beneficial traits. It is then expected that molecular biologists and plant breeders will have opportunities to use diverse lines in functional and comparative genomics, in mapping and cloning gene(s), and in applied plant breeding to diversify the genetic base of the breeding populations which should lead to the development of broad-based elite breeding lines/cultivars with superior yield and enhanced adaptation to diverse environments.

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