scholarly journals RAMAN-FLUORESCENT CHARACTERISTICS OF DIFFERENT ANATOMICAL AND TOPOGRAPHIC ZONES OF TEETH OF DIFFERENT FUNCTIONAL GROUPS

2019 ◽  
Vol 23 (5) ◽  
pp. 188-191
Author(s):  
Mikhail Timofeevich Alexandrov ◽  
E. F Dmitrieva ◽  
A. N Akhmedov ◽  
O. A Artemova ◽  
A. Potrivailo ◽  
...  
Keyword(s):  
Functional Groups ◽  
Tooth Enamel ◽  
In Vitro Study ◽  
High Sensitivity ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Degree Of Mineralization ◽  
Non Destructive ◽  
Test Objects

The aim of the study is to study the state of mineralization of various anatomical and topraphic zones of teeth for different functiona I groups and to justify its clinical feasibility. In a preclinical in vitro study on 20 model test objects of teeth (incisors, canines, premolars and painters), removed according to clinical indications, Raman-fluorescent study of the degree of mineralization of various anatomical and topographic zones of the tooth in different functional groups of teeth was carried out. Used APC Inspector M with a wavelength of532 nm probing radiation. The advantages of Raman fluorescence spectroscopy for determining the degree of mineralization of hard tooth tissues are objectivity (digital technology), expressiveness, non-invasiveness, simple and non-destructive control of the degree of mineralization/remineralization of hard tooth tissues, the ability to document and store information. In th e course of the study, a qualitative and quantitative analysis of the mineralization of various anatomical and topraphic zones of teeth for different functional groups of teeth was carried out. High sensitivity and reproducibility of the method allowed to reveal significant differences in mineralization of tooth enamel in the area of the cutting edge, equator and neck of the tooth. It is shown that these differences are manifested in other functional groups of teeth (p < 0.05).

scholarly journals THE USE OF RAMAN FLUORESCENCE TECHNOLOGY TO EVALUATE THE EFFECTIVENESS OF REMINERALIZATION OF SOLID TOOTH TISSUE

2018 ◽  
Vol 22 (5) ◽  
pp. 220-223
Author(s):  
Mikhail Timofeevich Alexandrov ◽  
M. A Polyakova ◽  
V. I Kukushkin ◽  
G. E Bagramova ◽  
K. S Babina ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
In Vitro Study ◽  
High Sensitivity ◽  
Qualitative And Quantitative Analysis ◽  
Lower Jaw ◽  
Fluorescence Diagnostics ◽  
Non Destructive ◽  
Tooth Tissue ◽  
532 Nm

The purpose of the study-to justify the effectiveness of the method of Raman-fluorescence diagnostics in the assessment of the remineralisation of the hard tissues of the tooth. In preclinical in vitro study on the model 25 the test object teeth (the incisors of the lower jaw) removed as clinically indicated, carried out Raman-fluorescence spectroscopy with a wavelength of probe radiation of 532 nm. The advantages of Raman-fluorescence spectroscopy are expressiveness, noninvasiveness, simple and non-destructive control of the degree of remineralization of hard tooth tissues [1-3]. In the course of the study, a qualitative and quantitative analysis of the content and distribution of hydroxyapatite in the enamel in the normal and with the use of remineralizing drugs was carried out. The high sensitivity and reproducibility of the method allowed us to assess the degree of mineralization of the tooth, the effectiveness of remineralizing drugs.

scholarly journals RESEARCH OF INFLUENCE OF SALIVARY AND Oral CLEANING HYGIENE ON INDICATORS OF MINERALIZATION OF HARD TOOTH TISSUES OF DIFFERENT FUNCTIONAL GROUPS

2019 ◽  
Vol 23 (3-4) ◽  
pp. 100-105
Author(s):  
Mikhail Timofeevich Alexandrov ◽  
E. F Dmitrieva ◽  
O. A Artemova ◽  
A. N Akhmedov
Keyword(s):  
Fluorescence Spectroscopy ◽  
Functional Groups ◽  
Oral Hygiene ◽  
In Vitro Study ◽  
Industrial Complex ◽  
Degree Of Mineralization ◽  
Non Invasive ◽  
Hard Tissues ◽  
Invasive Control

In vitro, investigate by the method of Raman fluorescence spectroscopy the effect of saliva and oral hygiene products on the mineralization indices of various anatomical and topographic areas of teeth for their various functional groups, to substantiate its clinical feasibility and effectiveness. Methods: in a preclinical in vitro study on 90 model test objects of teeth (incisors, premolars, and molars) removed according to clinical indications, a Raman fluorescence study of the degree of mineralization (Raman spectrum characteristics), dental hygiene conditions (plaque and intensity fluorescence) and evaluation of the effectiveness of remineralizing agents (films, suspensions) in various functional groups of teeth. For registration of the studied parameters, InSpektr M agro-industrial complex was used with a probe wavelength of 532 nm. The advantages of Raman fluorescence spectroscopy for determining the degree of mineralization and the hygienic state of tooth hard tissues are objectivity (digital technology), expressivity, non-invasiveness, simple and non-invasive control the degree of mineralization / demineralization of the hard tissues of the tooth and its hygienic state, the possibility of documenting and storing information (creating a database). Results: In the course of the study, a qualitative and quantitative analysis of the influence of saliva and oral hygiene products on the mineralization and hygienic condition of various functional groups of teeth was carried out.

scholarly journals X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples

2021 ◽  
Vol 22 (7) ◽  
pp. 3691
Author(s):  
Oliver Schmutzler ◽  
Sebastian Graf ◽  
Nils Behm ◽  
Wael Y. Mansour ◽  
Florian Blumendorf ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Irradiation Time ◽  
High Sensitivity ◽  
Nanoparticle Uptake ◽  
X Ray ◽  
Functionalized Gold Nanoparticles ◽  
Uptake Measurement ◽  
Prostate Tumor Cells ◽  
Non Destructive

Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.

scholarly journals Laser versus LED polymerization: Comparison of in vitro preventive sealing procedures

2019 ◽  
Vol 17 (1) ◽  
pp. 228080001881601
Author(s):  
Filiberto Mastrangelo ◽  
Isabella Perraro ◽  
Sabrina Mattia ◽  
Giuseppe Troiano ◽  
Khrystyna Zhurakivska ◽  
...  
Keyword(s):  
Laser Diode ◽  
Tooth Enamel ◽  
In Vitro Study ◽  
Third Molars ◽  
Fissure Sealant ◽  
Microscopic Evaluation ◽  
Group A ◽  
Group B ◽  
Metallization Process

Introduction: The technique of sealing is a widely accepted procedure for prevention of caries. The aim of our in vitro study was to compare the effect of two different curing units (traditional LED source and innovative laser diode lamp) on the integrity of fissure sealant material and its interface with tooth enamel. Materials and methods: Sixty healthy third molars were randomly assigned to two groups. In group A were teeth intended for polymerization by LED B lamp, and group B comprised teeth to be polymerized by an innovative laser diode. Both groups were treated with the traditional sealing technique, subjected to a metallization process, and analyzed by scanning electron microscope. Results: Micro-gaps between the sealant and the enamel were found in specimens in both A (43%) and B (40%) groups ( p=0.793), and sealant shrinkage was seen. Significant differences between the groups emerged in the percentage of perimetric micro-erosion sites (80% vs. 100%, p=0.010) and the presence of holes and micro-bubbles on the sealant surface (21% vs. 63%, p=0.001). Conclusions: Although macroscopic clinical polymerization occurred with both instruments, the microscopic evaluation showed significant differences between the studied groups in terms of perimetric micro-erosion sites and micro-bubbles, which were higher in laser-cured samples.

scholarly journals Simultaneous Determination of Acetylsalicylic Acid, Hydrochlorothiazide, Enalapril, and Atorvastatin in a Polypill-Based Quaternary Mixture by TLC

2018 ◽  
Vol 101 (3) ◽  
pp. 708-713
Author(s):  
Anna Maślanka ◽  
Mariusz Stolarczyk ◽  
Anna Apola ◽  
Anna Kwiecień ◽  
Urszula Hubicka ◽  
...  
Keyword(s):  
Acetylsalicylic Acid ◽  
Mobile Phase ◽  
High Sensitivity ◽  
Good Precision ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Treatment And Prevention ◽  
Quaternary Mixture ◽  
Prevention Of Cardiovascular Disease

Abstract A new chromatographic-densitometric method has been developed for the qualitative and quantitative determination of the active ingredients in a simulated mixture corresponding to the PolyIran polypill, composed of acetylsalicylic acid, hydrochlorothiazide (HCT), enalapril (ENA), and atorvastatin (ATR), whose efficacy in the treatment and prevention of cardiovascular disease has been documented in clinical trials. Chromatographic separation was performed using TLC silica gel 60 plates with fluorescent indicator F254 as the stationary phase and a mixture of n-hexane–ethyl acetate–methanol–water–acetic acid (8.4 + 8 + 3 + 0.4 + 0.2, v/v/v/v/v) as the mobile phase. Densitometric measurements were carried out at λ = 210 nm when determining ENA and at λ = 265 nm in the case of the other drugs. Peaks of examined substances were well separated in the recorded chromatograms, enabling the evaluation of the results in terms of both qualitative and quantitative analysis. The method was specific for the analyzed components and was characterized by high sensitivity. The LOD was between 0.043 and 0.331 μg/spot, and LOQ was between 0.100 and 0.942 μg/spot. Recovery was in the range of 97.02–101.34%. The linearity range was broad and ranged from 0.600 to 6.000 μg/spot for acetylsalicylic acid, from 0.058 to 1.102 μg/spot for HCT, from 0.505 to 6.560 μg/spot for ENA, and from 0.100 to 1.000 μg/spot for ATR. The method was characterized by good precision, with RSD values that ranged from 0.10 to 2.26%.

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