scholarly journals RESEARCH OF INFLUENCE OF SALIVARY AND Oral CLEANING HYGIENE ON INDICATORS OF MINERALIZATION OF HARD TOOTH TISSUES OF DIFFERENT FUNCTIONAL GROUPS

Author(s):  
Mikhail Timofeevich Alexandrov ◽  
E. F Dmitrieva ◽  
O. A Artemova ◽  
A. N Akhmedov

In vitro, investigate by the method of Raman fluorescence spectroscopy the effect of saliva and oral hygiene products on the mineralization indices of various anatomical and topographic areas of teeth for their various functional groups, to substantiate its clinical feasibility and effectiveness. Methods: in a preclinical in vitro study on 90 model test objects of teeth (incisors, premolars, and molars) removed according to clinical indications, a Raman fluorescence study of the degree of mineralization (Raman spectrum characteristics), dental hygiene conditions (plaque and intensity fluorescence) and evaluation of the effectiveness of remineralizing agents (films, suspensions) in various functional groups of teeth. For registration of the studied parameters, InSpektr M agro-industrial complex was used with a probe wavelength of 532 nm. The advantages of Raman fluorescence spectroscopy for determining the degree of mineralization and the hygienic state of tooth hard tissues are objectivity (digital technology), expressivity, non-invasiveness, simple and non-invasive control the degree of mineralization / demineralization of the hard tissues of the tooth and its hygienic state, the possibility of documenting and storing information (creating a database). Results: In the course of the study, a qualitative and quantitative analysis of the influence of saliva and oral hygiene products on the mineralization and hygienic condition of various functional groups of teeth was carried out.

Author(s):  
Mikhail Timofeevich Alexandrov ◽  
E. F Dmitrieva ◽  
A. N Akhmedov ◽  
O. A Artemova ◽  
A. Potrivailo ◽  
...  

The aim of the study is to study the state of mineralization of various anatomical and topraphic zones of teeth for different functiona I groups and to justify its clinical feasibility. In a preclinical in vitro study on 20 model test objects of teeth (incisors, canines, premolars and painters), removed according to clinical indications, Raman-fluorescent study of the degree of mineralization of various anatomical and topographic zones of the tooth in different functional groups of teeth was carried out. Used APC Inspector M with a wavelength of532 nm probing radiation. The advantages of Raman fluorescence spectroscopy for determining the degree of mineralization of hard tooth tissues are objectivity (digital technology), expressiveness, non-invasiveness, simple and non-destructive control of the degree of mineralization/remineralization of hard tooth tissues, the ability to document and store information. In th e course of the study, a qualitative and quantitative analysis of the mineralization of various anatomical and topraphic zones of teeth for different functional groups of teeth was carried out. High sensitivity and reproducibility of the method allowed to reveal significant differences in mineralization of tooth enamel in the area of the cutting edge, equator and neck of the tooth. It is shown that these differences are manifested in other functional groups of teeth (p < 0.05).


2018 ◽  
Vol 22 (2) ◽  
pp. 75
Author(s):  
Budi Saksono

      In the previous paper, we had succeeded in developing an early detection system of dengue viruses using Sugar liganded Gold Nano Particle (SGNP) only from 6 μL serum. It has been reported that dengue virus is also detected in the saliva and urine of the patient. The evidences lead to the possibility of developing non-invasive methods of dengue virus detection. In this in vitro study, we evaluated the utility of SGNP to capture and concentrate dengue virion in 10% saliva solution. The results showed that dengue virion was successfully detected in 10% of saliva solution. Analysis of virion stability during storage showed that virions in salivary samples were stable up to 3 days at temperature wherease the RNA has significantly degraded. Although still a preliminary study, the data obtained show the prospect of SGNP as a non-invasive dengue virus detection method, as well as the development of POC (Point of Care) method. Clinical trials using saliva from dengue viruses infected patients need to be done to prove the effectiveness of the SGNP method.


2020 ◽  
Vol 14 (4) ◽  
pp. 7600-7608
Author(s):  
W. A. A Saad ◽  
Mohd Azuwan Mat Dzahir ◽  
Yamamoto Shinichirou ◽  
Mohamed Hussein ◽  
Maziah Mohamad ◽  
...  

The change of the spinal curvature in completing a variety of daily tasks is essential to independent living. There is still a lack of studies highlighting the lumbar segmental contribution during sit-to-stand (STS) and stand-to-flexion (STF) using non-invasive study. The purpose of this study is to compare the spine kinematics by defining lumbar as a single and multi-segmental during continuous daily motion in healthy Asian adults using a non-invasive approach. During STS, most subjects implemented kyphotic lumbar curve during the early stage of motion which revealed poor posture implementation and significant differences in the lumbar kinematics which were only noticeable at specific phases between both approaches. A significant difference in multi-segmental behaviour was observed only at the end of the motion. All three segments displayed different time responses during the transition from kyphotic to lordotic curve. Passive/delayed behavior within the lower lumbar segment was observed between 0-50% of motion completion. During STF, statistically significant differences were found between assuming lumbar as a single and multi-segment in all phases. This in vitro study identified characteristic motion patterns in the lumbar spine during daily motions. The results provided a clear description of the healthy spinal condition of adults and may serve to identify specific multi-segmental contribution.


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