scholarly journals Genetic Characterization of Domesticated F1 Generation in Humpback Grouper (Cromileptes altivelis)

2007 ◽  
Vol 5 (1) ◽  
pp. 87
Author(s):  
Ratu Siti Aliah ◽  
. Wahidah ◽  
K. Sumantadinata ◽  
Estu Nugroho ◽  
O. Carman
Keyword(s):  
First Generation ◽  
Genetic Characterization ◽  
Haplotype Diversity ◽  
Breeding Strategy ◽  
Loop Region ◽  
Pcr Rflp ◽  
D Loop ◽  
F1 Generation ◽  
Mtdna Haplotype

<p>First generation (F1) of hatchery produced humpback grouper (<em>Cromileptes altivelis</em>) has been characterized genetically in order to serve the information of their status in related to their breeding strategy. PCR-RFLP method was used to detect the variation of mtDNA D-loop region of F1 population at BBPBL Lampung and BBAP Situbondo. The result of study showed that reducing of haplotype diversity had been arised from broodstock (0.8548) to F1 generation population (0.7473; 0.7273; and 0.6947, respectively).  Genetic divergence that had found between population BBPBL Lampung and BBAP Situbondo make it possible to do outbreeding in order to get its heterosis's effect.</p> <p>Keywords: mtDNA, haplotype diversity, genetic differentiation, <em>Cromileptes altivelis</em></p> <p> </p> <p>ABSTRAK</p> <p>Ikan kerapu tikus (<em>Cromileptes altivelis</em>) generasi pertama (F1) hasil domestikasi di hatchery telah dikarakterisasi secara genetik untuk menyediakan informasi status sehubungan dengan program pemuliaannya.  Metode PCR-RFLP digunakan untuk mendeteksi variasi sekuens D-loop mtDNA ikan kerapu tikus F1 yang diproduksi di BBPBL Lampung dan BBAP Situbondo.  Hasil penelitian menunjukkan bahwa telah terjadi penurunan keragaman haplotipe dari induk (0,8548) ke populasi generasi F1 (masing-masing 0,7473; 0,7273; dan 0,6947).  Adanya keragaman genetik antara populasi ikan kerapu tikus di BBPBL dan BBAP Situbondo memungkinkan dilakukannya <em>outbreeding</em> untuk mendapatkan efek heterosis.</p> <p>Kata kunci: mtDNA, keragaman haplotipe, diferensiasi genetik, <em>Cromileptes altivelis</em></p>

scholarly journals Characterization of genetic diversity of an invasive Lessepsian Migrant, Nemipterus randalli Russell, 1986 from its native and non-native regions using mitochondrial DNA marker

2020 ◽  
Author(s):  
MURUGESAN SRIHARI ◽  
SUSANTHI SILPA ◽  
ANNAM PAVAN-KUMAR ◽  
YARON TIKOCHINSKI ◽  
DANIEL GOLANI ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Mediterranean Sea ◽  
Haplotype Diversity ◽  
Loop Region ◽  
Geographical Population ◽  
D Loop ◽  
The Mediterranean ◽  
Network Tree

This study assessed and compared the genetic diversity of Nemipterus randalli across its native and non-native regions analysing the mitochondrial DNA D-loop region. Including all the geographical population samples, 68 haplotypes were observed with an average haplotype diversity value of 0.92±0.04. Relatively, a smaller number of haplotypes was observed in the invasive range of the Mediterranean Sea. All other native geographical samples showed high haplotype and nucleotide diversity values. A significant high genetic differentiation value was observed between the native population samples of India and the invasive samples of the Mediterranean Sea. In the median-joining network tree, N. randalli from the Mediterranean Sea and the Red Sea formed a single haplogroup while other samples from India are clustered into two haplogroups.

scholarly journals Genetic characterization of Laotian native cattle using mtDNA haplotype and loci associated with economical traits, coat color, and a hereditary disorder

2017 ◽  
Vol 45 (2) ◽  
pp. 43-48 ◽  
Author(s):  
Yu OKUDA ◽  
Taishi KANII ◽  
Yoshio YAMAMOTO ◽  
Bounthavone KOUNNAVONGSA, ◽  
Soukanh KEONOUCHANH ◽  
...  
Keyword(s):  
Coat Color ◽  
Genetic Characterization ◽  
Native Cattle ◽  
Mtdna Haplotype

scholarly journals Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Widya Pintaka Bayu Putra ◽  
Saiful Anwar ◽  
Syahruddin Said ◽  
Romanos Albert Adhitya Indarto ◽  
Putri Wulandari
Keyword(s):  
Genetic Characterization ◽  
Rare Allele ◽  
Basic Information ◽  
Dominant Allele ◽  
West Java ◽  
Pcr Rflp ◽  
Molecular Selection ◽  
Lep Gene ◽  
Informative Content

The Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecular selection to improve carcass traits in beef cattle. This research was carried out to identify the genetic characterization of TG and LEP genes from 47 heads of Pasundan cows at West Java using PCR-RFLP method. Research shows that TG gene of Pasundan cattle is monomorphic with C allele as the dominant allele (1.00). However, LEP gene of Pasundan cattle is polymorphic with C allele as the dominant allele (0.98) and T as the rare allele (0.02). The polymorphic informative content (PIC) and numberof effective allele (ne) values in the LEP gene in the animal studied were 0.04 and 1.04 respectively. It was concluded that TG/BstYI and LEP/Sau3AI gene in the present study can not be used as molecular selection in Pasundan cattle. These results are important as the basic information for preparing the molecular selection program in the future.

Polymorphisms of genes required for methionine synthesis and DNA methylation influence mitochondrial DNA methylation

Epigenomics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1003-1012
Author(s):  
Andrea Stoccoro ◽  
Pierpaola Tannorella ◽  
Lucia Migliore ◽  
Fabio Coppedè
Keyword(s):  
Dna Methylation ◽  
Mitochondrial Dna ◽  
Exploratory Study ◽  
High Resolution Melting ◽  
Late Onset ◽  
Loop Region ◽  
Pcr Rflp ◽  
D Loop ◽  
One Carbon Metabolism ◽  
Methionine Synthesis

Aim: Impaired methylation of the mitochondrial DNA and particularly in the regulatory displacement loop (D-loop) region, is increasingly observed in patients with neurodegenerative disorders. The present study aims to investigate if common polymorphisms of genes required for one-carbon metabolism ( MTHFR, MTRR, MTR and RFC-1) and DNA methylation reactions ( DNMT1, DNMT3A and DNMT3B) influence D-loop methylation levels. Materials & methods: D-loop methylation data were available from 133 late-onset Alzheimer’s disease patients and 130 matched controls. Genotyping was performed with PCR-RFLP or high resolution melting techniques. Results: Both MTRR 66A > G and DNMT3A -448A > G polymorphisms were significantly associated with D-loop methylation levels. Conclusion: This exploratory study suggests that MTRR and DNMT3A polymorphisms influence mitochondrial DNA methylation; further research is required to better address this issue.

scholarly journals Maternal variability of Croatian Spotted goat (Capra hircus)

2019 ◽  
Vol 64 (No. 6) ◽  
pp. 248-254
Author(s):  
Ivana Drzaic ◽  
Ino Curik ◽  
Dinko Novosel ◽  
Vlatka Cubric-Curik
Keyword(s):  
Mitochondrial Dna ◽  
Nucleotide Diversity ◽  
Population Expansion ◽  
Capra Hircus ◽  
Phylogeographic Structure ◽  
Goat Breed ◽  
Loop Region ◽  
D Loop ◽  
Mtdna Diversity

Abstract: This study provides the first characterization of maternal ancestry and mitochondrial DNA (mtDNA) diversity in the Croatian Spotted goat (CSG), the most important autochthonous goat breed in Croatia. CSG (n = 25) were randomly sampled from seven herds and a 660-bp fragment from the mtDNA D-loop region was sequenced. Those sequences were compared with 122 corresponding GenBank sequences from goat populations in Albania, Austria, Egypt, Greece, Italy, Romania and Switzerland. CSG showed a great polymorphism (only three out of 17 haplotypes were shared) with high a haplotype (Hd = 0.967 ± 0.019) and nucleotide diversity (π = 0.01305 ± 0.00068). When compared with Mediterranean and ancient goats, all of the 25 CSG were randomly scattered inside haplogroup A showing the weak phylogeographic structure with within-breed variance accounting for 91.76% of the genetic variation. In addition, population expansion tests (mismatch distribution and Fu’s Fs statistic) supported these results suggesting at least one population expansion.

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