scholarly journals Oxidative Stress and Inflammatory Biomarkers Analysis of Biofield Treated Proprietary Test Formulation in Heart Tissues in Cecal Slurry, LPS and E. Coli-induced Systemic Inflammatory Response Syndrome (SIRS) in Sprague Dawley Rats

2021 ◽  
Vol 6 (4) ◽  
Author(s):  
Snehasis Jana
Keyword(s):  
Oxidative Stress ◽  
Systemic Inflammatory Response Syndrome ◽  
Inflammatory Response ◽  
Systemic Inflammatory Response ◽  
Inflammatory Biomarkers ◽  
Sprague Dawley ◽  
E Coli ◽  
Test Formulation ◽  
Sprague Dawley Rats

scholarly journals Antioxidant and Anti-Inflammatory Activities of Biofield Energy Treated Proprietary Test Formulation in Brain Tissues in Cecal Slurry, LPS and E. Coli-Induced Systemic Inflammatory Response Syndrome (SIRS) in Sprague Dawley Rats

2021 ◽  
Vol 1 (3) ◽  
pp. 13-25
Author(s):  
Mahendra Kumar Trivedi ◽  
Alice Branton ◽  
Dahryn Trivedi ◽  
Snehasis Jana
Keyword(s):  
Systemic Inflammatory Response Syndrome ◽  
Inflammatory Response ◽  
Systemic Inflammatory Response ◽  
Sprague Dawley ◽  
E Coli ◽  
Test Formulation ◽  
Sprague Dawley Rats ◽  
Tnf Α ◽  
Per Se ◽  
Anti Inflammatory

The study was aimed to evaluate the antioxidant and anti-inflammatory activity of the Biofield Energy Treated Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on Cecal Slurry, LPS and E. coli-induced systemic inflammatory response syndrome (SIRS) model in Sprague Dawley rats. In this experiment, different antioxidants biomarkers such as myeloperoxidase (MPO), superoxide dismutase (SOD), lipid peroxidase (LPO) and cytokines like interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), macrophage inflammatory protein-2 (MIP-2) were analysed using ELISA assay in brain homogenate. A test formulation was formulated including minerals (magnesium, zinc, calcium, selenium, and iron), vitamin C, B6, E, B12, D3, β-carotene, cannabidiol isolate,and Panax ginseng extract. The component of the test formulation were divided into two parts; one section was defined as the untreated, while the other portion of each constituent and three group of animals received Biofield Energy Healing/Blessing Treatment remotely for about 3 minutes by Mr. Mahendra Kumar Trivedi, a renowned spiritual Energy Healer. The level of MPO was significantly (p≤0.001) reduced by 19.43%, 34.91%, 25.43%, 25.29% and 30.33% in the G5 (Cecal Slurry, LPS and E. coli + Biofield Energy Treated test formulation); G6 (Cecal Slurry, LPS and E. coli + Biofield Energy Treatment per se to animals from day -15); G7 (Cecal Slurry, LPS and E. coli + Biofield Energy Treated test formulation from day -15); G8 (Cecal Slurry, LPS and E. coli + Biofield Energy Treatment per se + Biofield Energy Treated/Blessed test formulation from day -15), and G9 (Cecal Slurry, LPS and E. coli + Biofield Energy Treatment per se animals + untreated test formulation) groups, respectively as compared to the untreated test formulation (G4) group. Moreover, the level of SOD was significantly increased by 45.02% (p≤0.001), 16.59%, and 35.99% (p≤0.001) in the G6, G7, and G9 groups, respectively as compared to G4 group. The level of TNF-α was significantly decreased by 12.66%, 46.92% (p≤0.001), 26.57% (p≤0.001), 23.22% (p≤0.001), and 54.28% (p≤0.001) in G5, G6, G7, G8, and G9 groups, correspondingly with reference to G4 group. Moreover, the level of IL-6 was significantly (p≤0.001) decreased by 37.51%, 20.28%, 21.55%, and 33.4% in the G6, G7, G8, and G9 groups, respectively as compared to the G4 group. Additionally, the level of MIP-2 was significantly (p≤0.001) reduced by 47.97%, 17.08%, 20.16% and 26.84% in the G6, G7, G8, and G9 groups, respectively as compared to the G4 group. Together, the data imply the antioxidant and anti-inflammatory potential of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various inflammatory conditions that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of injuries. Therefore, the results described a significant reduction of inflammation-related disease progression rate and its complications in the preventive maintenance groups (viz. G6, G7, G8, and G9).

INFLAMMATORY BIOMARKERS IN THE SYSTEMIC INFLAMMATORY RESPONSE SYNDROME (SIRS): ARE THERE DIFFERENCES IN PATIENTS THAT BECOME SEPTIC?

2004 ◽  
Vol 32 (Supplement) ◽  
pp. A160
Author(s):  
Steven B Johnson ◽  
Alan Cross ◽  
Jeff Hasday ◽  
Grant Bochicchio ◽  
Carl Shanholtz ◽  
...  
Keyword(s):  
Systemic Inflammatory Response Syndrome ◽  
Inflammatory Response ◽  
Systemic Inflammatory Response ◽  
Inflammatory Biomarkers

Oxidative stress in critically ill patients with systemic inflammatory response syndrome

2002 ◽  
Vol 30 (8) ◽  
pp. 1782-1786 ◽  
Author(s):  
José M. Alonso de Vega ◽  
Julián Díaz ◽  
Enrique Serrano ◽  
Luis F. Carbonell
Keyword(s):  
Oxidative Stress ◽  
Systemic Inflammatory Response Syndrome ◽  
Inflammatory Response ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Systemic Inflammatory Response

Inhibiting adenosine deaminase modulates the systemic inflammatory response syndrome in endotoxemia and sepsis

2002 ◽  
Vol 282 (5) ◽  
pp. R1324-R1332 ◽  
Author(s):  
Simon Adanin ◽  
Igor V. Yalovetskiy ◽  
Beth A. Nardulli ◽  
Albert D. Sam ◽  
Živojin S. Jonjev ◽  
...  
Keyword(s):  
Systemic Inflammatory Response Syndrome ◽  
Inflammatory Response ◽  
Adenosine Deaminase ◽  
Elevated Serum ◽  
Systemic Inflammatory Response ◽  
E Coli ◽  
Adenosine Receptor Antagonist ◽  
Endogenous Adenosine ◽  
Tnf Α ◽  
Sepsis Induction

By pharmacological manipulation of endogenous adenosine, using chemically distinct methods, we tested the hypothesis that endogenous adenosine tempers proinflammatory cytokine responses and oxyradical-mediated tissue damage during endotoxemia and sepsis. Rats were pretreated with varying doses of pentostatin (PNT; adenosine deaminase inhibitor) or 8-sulfophenyltheophylline (8-SPT; adenosine receptor antagonist) and then received either E. coli endotoxin (lipopolysaccharide; 0.01 or 2.0 mg/kg) or a slurry of cecal matter in 5% dextrose in water (200 mg/kg). Resultant levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-10 were measured in serum and in liver and spleen. Untreated, 2 mg/kg lipopolysaccharide elevated serum TNF-α, IL-1β, and IL-10. PNT dose dependently attenuated, without ablating, the elevation in serum TNF-α and IL-1β and raised liver and spleen IL-10. PNT also attenuated elevation of TNF-α in serum, liver, and spleen at 4 and 24 h after sepsis induction, and 8-SPT resulted in higher proinflammatory cytokines. Modulating endogenous adenosine was also effective in exacerbated (8-SPT) or diminished (PNT) tissue peroxidation. Survival from sepsis was also improved when PNT was used as a posttreatment. These data indicate that endogenous adenosine is an important modulatory component of systemic inflammatory response syndromes. These data also indicate that inhibition of adenosine deaminase may be a novel and viable therapeutic approach to managing the systemic inflammatory response syndrome without ablating important physiological functions.

scholarly journals Prospective evaluation of S100A12 and S100A8/A9 (calprotectin) in dogs with sepsis or the systemic inflammatory response syndrome

2019 ◽  
Vol 31 (4) ◽  
pp. 645-651 ◽  
Author(s):  
Brittany E. Thames ◽  
James W. Barr ◽  
Jan S. Suchodolski ◽  
Jörg M. Steiner ◽  
Romy M. Heilmann
Keyword(s):  
Systemic Inflammatory Response Syndrome ◽  
Inflammatory Response ◽  
Hospital Admission ◽  
Surrogate Marker ◽  
Systemic Inflammatory Response ◽  
Inflammatory Biomarkers ◽  
Healthy Controls ◽  
Prospective Evaluation ◽  
Lower Serum ◽  
Patient Groups

Pattern recognition receptors (e.g., S100A12 or S100A8/A9) hold promise as inflammatory biomarkers. We prospectively determined and compared serum S100A12 and S100A8/A9 concentrations in dogs with sepsis ( n = 11) or systemic inflammatory response syndrome (SIRS; n = 8) over a 3-d period with each other, healthy controls ( n = 50), and other clinical and clinicopathologic variables. Serum S100A12 and S100A8/A9 concentrations were significantly higher in dogs with sepsis or SIRS (all p < 0.05) at the time of hospital admission (day 1) compared to healthy controls, with no differences between patient groups. However, septic dogs had significantly lower serum S100A12 concentrations on day 2 and day 3 (both p < 0.05) compared to dogs with SIRS. Likewise, dogs with sepsis had significantly lower S100A8/A9 concentrations on day 2 ( p < 0.05). Neither serum S100A12 nor S100A8/A9 concentrations were associated with survival to discharge. Our results suggest a differential expression of the S100/calgranulins between dogs with sepsis and those with SIRS. Serum S100A12 or S100A8/A9 concentration at the time of hospital admission did not differentiate dogs with sepsis from those with SIRS, but the trend of S100/calgranulin concentrations during the following 24–48 h may be a useful surrogate marker for differentiating sepsis from SIRS.

CHARACTERISTICS OF ACTIVE OXYGEN FORMS AND ANTIOXIDANTS AT EXPERIMENTAL METABOLIC SYNDROME AND ITS REMODELING BY GRAPE POLYPHENOLS

2019 ◽  
pp. 103-113
Author(s):  
Yu.I. Shramko ◽  
A.V. Kubyshkin ◽  
I.I. Fomochkina ◽  
L.L. Aliev ◽  
D.V. Chegodar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
Superoxide Dismutase ◽  
Systemic Inflammatory Response Syndrome ◽  
Inflammatory Response ◽  
Thiobarbituric Acid ◽  
Active Oxygen ◽  
Systemic Inflammatory Response ◽  
Active Oxygen Forms ◽  
Grape Polyphenols

Adipose tissue in obesity produces more active oxygen forms and inflammatory cytokines, which results in the development of a systemic inflammatory response syndrome (SIRS) and a metabolic syndrome (MS). Recently, there has been a growing interest in natural products for oxidative stress remodeling. Therefore, it is relevant to study grape polyphenols (GPs) to develop certain techniques for oxidative stress prevention and treatment. The goal of the paper is to examine antioxidant profiles in MS, and the efficacy of its pathogenetic correction with GPs. Materials and Methods. The authors estimated the ratio of peroxidase (PA) activity and thiobarbituric acid active products (TBA-APs), and the ratio of ceruloplasmin (C) and PA. Results. The authors observed stereotypical changes in the systems of antioxidant and antiproteolytic protection in MS. Thus, with MS, there was recorded a decrease in PA/TBA-AP ratio (2.3 compared with 16.6 in norm) and an increase in C/PA ratio (3.17 compared with 0.62 in norm). In the case of “Fenokor” administration for MS remodeling, the level of superoxide dismutase became normal. Moreover, the authors observed the highest level of TBA-active products in experimental animals. MS remodeling with Fenokor was effective: PA/TBA ratio was 2 times higher than in the control, and C/PA ratio was 0.86. Catalase and superoxide dismutase levels were 5 and 1.3 times higher than in norm, respectively. Conclusion. Normalization of PA/TBA-AP and C/PA ratios under “Fenokor” allows us to GPs with a high polyphenol content to correct antioxidant status and reduce systemic inflammatory response syndrome. Keywords: oxidative stress; grape polyphenols. Жировая ткань при ожирении производит большее количество активных форм кислорода и воспалительных цитокинов, что приводит к развитию синдрома системной воспалительной реакции (ССВР) и метаболического синдрома (МС). В последнее время возрастает интерес к использованию продуктов натурального происхождения для коррекции оксидативного стресса. Поэтому представляется актуальным изучение полифенольных продуктов переработки винограда (ПППВ) для разработки методов профилактики и терапии проявлений окислительного стресса. Цель – изучение антиоксидантных профилей при МС, а также эффективности его патогенетической коррекции ПППВ. Материалы и методы. Оценивали отношение активности пероксидазы (ПА) и активных продуктов тиобарбитуровой кислоты (ТБК-АП), а также церулоплазмина (Ц) и ПА. Результаты. Показана определённая стереотипность изменений в системах антиоксидантной и антипротеолитической защиты при МС. Так, при МС регистрировалось снижение ПА/ТБК-АП (2,3 по сравнению с 16,6 в норме) и повышение Ц/ПА (3,17 по сравнению с 0,62 в норме). В случае применения «Фэнокора» для коррекции проявлений МС происходила нормализация содержания супероксиддисмутазы, наблюдался самый высокий среди экспериментальных животных уровень ТБК-активных продуктов. Коррекция МС с помощью «Фенокора» была эффективна: соотношение ПА/ТБА было в 2 раза выше, чем в контроле, а отношение Ц/ПА составляло 0,86. Уровни каталазы и супероксиддисмутазы были в 5 и 1,3 раза выше нормы соответственно. Заключение. Нормализация отношений ПА/ТБК-АП и Ц/ПА при применении препарата «Фэнокор» позволяет использовать ПППВ с высоким содержанием полифенолов для коррекции антиоксидантного статуса и снижения проявлений ССВР. Ключевые слова: оксидативный стресс, полифенолы винограда.

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