scholarly journals BLOOD PROFILES OF GREEN SEA TURTLE BEFORE AND AFTER THE REHABILITATION

2020 ◽  
Vol 9 (6) ◽  
pp. 930-939
Author(s):  
Ida Bagus Windia Adnyana ◽  
Ida Bagus Nararya Primastana Adnyana ◽  
Siswanto Siswanto

Penyu adalah satwa yang terancam punah, sehingga upaya konservasinya perlu ditingkatkan. Kegiatan yang berhubungan dengan konservasi dimaksud adalah rehabilitasi penyu pascamengalami periode out of the water sebelum dilepasliarkan kembali ke alam bebas. Penelitian ini bertujuan untuk mengetahui perbedaan profil dan indeks eritrosit penyu hijau (Chelonia mydas) sebelum dan sesudah mengalami rehabilitasi di Turtle Conservation and Education Center (TCEC) di Pulau Serangan, Denpasar. Penelitian ini menggunakan sampel Sembilan ekor penyu hijau hasil sitaan Polisi Sektor Kuta, Badung, Bali. Sebanyak 2,5 mL darah perifer penyu hijau diambil dari sinus cervicalis dorsalis disimpan di tabung berisikan antikoagulan litium heparin dan dilanjutkan dengan pemeriksaan hematologi. Penentuan nilai total eritrosit dihitung dengan hemositometer. Kadar hemoglobin (Hb) diukur menggunakan Hemoglobinometer Sahli, sedangkan kadar Packed Cell Volume (PCV) ditentukan dengan metode mikrohematokrit. Indeks eritrosit yang meliputi Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH) dan Mean Corpuscular Haemoglobin Concentration (MCHC) dihitung dengan rumus konvensional yang ditentukan untuk itu. Data hasil penelitian dianalisis secara deskriptif kuantitatif menggunakan uji T berpasangan. Nilai profil darah (Packed Cell Volume, total eritrosit, Hemoglobin) mengalami peningkatan signifikan pasca rehabilitasi dengan nilai rerata hematokrit sebelum rehabilitasi sebesar 28,4±3,09 menjadi 31,7±2,87, nilai total eritrosit sebelum rehabilitasi sebesar 0,44±0,10 menjadi 0,56±0,15, nilai Hemoglobin sebelum rehabilitasi sebesar 6,3±1,28 menjadi 7,53±0,73 serta hasil perhitungan secara statistika dengan uji T-berpasangan menunjukkan profil darah penyu hijau sebelum dan sesudah rehabilitasi memiliki perbedaan yang signifikan yang berarti proses rehabilitas yang dilakukan di Turtle Conservation and Education Center, Serangan berhasil.

1967 ◽  
Vol 18 (3) ◽  
pp. 523 ◽  
Author(s):  
C Dobson

Variations in the numbers of erythrocytes and white cells within the blood of sheep given various doses of Oesophagostomum columbianum larvae were studied over a period of 10 weeks. The sheep showed losses of red cells and haemoglobin, and the packed cell volume was diminished after infestation with O. columbianum. These effects increased in severity as the infesting dose was increased; the resistant state of the host also affected the blood picture. In all infestations the calculated absolute values for the blood, i.e. mean corpuscular haemoglobin (MCH), mean corpuscular volume (MCV), and mean corpuscular haemoglobin concentration (MCHC), did not significantly change except in the heavier infestations, where MCV increased for a time. The type of anaemia which predominated throughout these experiments was normocytic-normochromic. Where the MCV increased for a period, the anaemia was of the macrocytic-normochromic type. During the first infestation with 2000 larvae the anaemias tended to become microcytiohyperchromic, but these changes were not significant. Gross observations on the gut indicated that one of the factors influencing the development of anaemias was the extensive tissue damage caused by the larvae, which resulted in chronic bleeding from the mucosa. This suggestion was supported by the fact that anaemias also developed during second infestations when there was no adult worm population. The white cell counts from a number of these sheep were also recorded. The most significant changes occurred after second infestation, although a general increase was noted after all first infestations as well; prominent increases occurred in the agranulocytes and the eosinophil leucocytes. The neutrophil leucocyte counts fell throughout all the infestation. All the normal values obtained for the haemoglobin concentration, packed cell volume, red cell counts, MCV, MCH, and MCHC and white cell counts compared favourably with the values cited by other workers.


2012 ◽  
Vol 51 (No. 1) ◽  
pp. 29-34 ◽  
Author(s):  
K. Hauptmanova ◽  
M. Maly ◽  
I. Literak

The haematological parameters of the common pheasant (Phasianus colchicus), the dynamics of changes in blood count parameters with regard to the season of the year and reproduction status, and the effects of sex and body condition on the haematological parameters of blood were studied. In total, 574 blood samples were collected. The samples were collected from pheasants from the age of 6 months, prior to the laying period, and after the laying period. Packed cell volume and haemoglobin concentration significantly increased in males during the period of growth and decreased during the period of reproductive activity. Significantly higher values were detected in males compared to females in erythrocyte count, packed cell volume, haemoglobin concentration and in mean corpuscular haemoglobin concentration. A statistically significant positive relationship was detected between weight and the values of most of the parameters related to red blood cells.


2020 ◽  
Vol 46 (4) ◽  
pp. 206-210
Author(s):  
H. O. Uzegbu ◽  
I. Annatte ◽  
D. Onunkwo ◽  
E. K. Ndelekwute ◽  
E. Ewa

A feed trial was conducted with a total of 30 pigs to evaluate the potential of mixed fresh forages (Panicum maximum and Calopogonium mucunoids) on pigs. The trial lasted for 63 days. Five diets were formulated which contains 0, 10, 15, 20 and 25% level of grass and legume forage. Each diet formed a treatment which was offered to the pigs. Each treatment was replicated thrice with 2 pigs per replicate, arranged in a completely randomized design (CRD). The pigs were fed 4% of their body weight and water was given ad-libitum. The blood composition of the pigs in terms of packed cell volume, haemoglobin, white blood cell, mean corpuscular haemoglobin and mean corpuscular concentration. Results showed significant differences (P<0.05) in white blood cell (17.70 – 23.30) and mean corpuscular heamoglobin (15.83 – 18.03) and mean corpuscular volume (48.00 – 53.16(mg/100ml) and there is no treatment effect on observed in packed cell volume (36.00 – 42.00%), haemoglobin (12.00 – 14.00) and mean corpuscular haemoglobin concentration (33.25 – 33.41%). Recommended level of inclusion of the mixed forage (Panicum maximum and Calopogonium mucunoids) in the diet of pigs should not exceed 20%.


2020 ◽  
Vol 42 (1) ◽  
Author(s):  
T. Ahemen ◽  
I. I. Bitto ◽  
O. I. A. Oluremi ◽  
F. O. I. Anugwa

A study was conducted to investigate the effects of feeding graded levels of cassava leaf meal (CLM) on genital tract morphometry and haematology of twenty 8-week old male rabbits weighing between 815 to 910g. The rabbits were randomly assigned to four dietary treatments viz: T1 (control), T2, T3 and T4 with CLM inclusion levels of 0%, 9%, 18%, and 27%, respectively after balancing for live weight. Each rabbit was individually caged and served as a replicate in a completely randomized design. The feeding trial lasted for sixteen (16) weeks. Three (3) rabbits per treatment were slaughtered and evaluated for genital tract morphometry and haematological characteristics. The results showed that CLM had no significant effect (P>0.05) on testes, corpus, cauda, ductus and tunic weights. Positive relationships were observed between CLM in the diets and testes weight (r = 0.42; P<0.05) as well as cauda weights (r = 0.96; P<0.01). No significant effect (P>0.05) of diets were observed on packed cell volume, haemoglobin, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, mean corpuscular volume, platelets, white blood cells, neutrophils, lymphocytes, monocytes basophils and eosinophils. However negative relationships between levels of inclusion of CLM in diets and red blood cell, packed cell volume and haemoglobin were observed. It was concluded that the inclusion of up to 27% of CLM in bucks' diets is not detrimental to good health and normal reproductive tract development. It is recommended that further studies on the feeding potentials of cassava leaf meal at higher levels of inclusion and actual reproductive performance be investigated.


1992 ◽  
Vol 40 (4) ◽  
pp. 355 ◽  
Author(s):  
PBS Spencer ◽  
R Speare

The haematology of a colony of wild allied rock-wallabies, Petrogale assimilis Ramsay, 1877, from north-western Queensland was studied for 20 months. Wallabies were repeatedly trapped at monthly intervals and 511 blood samples from 96 animals were examined, making this the most comprehensive study of the haematology of any macropod species. The morphology of the red blood cells and leucocytes was similar to those of other marsupials. Large and small lymphocytes were recognised. Binucleate lymphocytes were found in 7.2% of blood smears. Normal haematological values were within the published ranges for other marsupials. However, the average white blood cell count (mean +/- s.e.: 9.03 +/- 0.92 X 10(9) L-1) was close to the upper end of the range for macropods. The normal (mean +/- s.e.) red blood cell count (5.24 + 0.15 X 10(12) L-1), packed cell volume (0.4291 +/- 0.019 L L-1) and haemoglobin concentration (14.07 +/- 0.41 g dL-1) of male wallabies were higher than those of females (4.79 +/- 0.15 X 10(12) L-1, 0.3952 +/- 1.19 L L-1, and 12.95 +/- 0.41 g dL-1, respectively). No gender differences were detected in the white blood cell count (9.03 +/- 0.92 x 10(9) L-1), mean corpuscular volume (82.28 +/- 1.35 fL), mean corpuscular haemoglobin (26.98 +/- 0.57 pg) or mean corpuscular haemoglobin concentration (32.82 +/- 0.57 g L 1). The red blood cell count, packed cell volume, haemoglobin concentration, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and the absolute neutrophil count in juveniles and subadults increased with age. The neutrophil to lymphocyte ratio was less than 1.0 in pouch young and approached 1.0 in adults. The red blood cell count, packed cell volume and haemoglobin concentration of lactating females did not vary with age of pounch young.


1980 ◽  
Vol 8 (3) ◽  
pp. 356-358 ◽  
Author(s):  
N. S. Agar ◽  
G. B. H. Lewis

No significant changes were found in packed cell volume, haemoglobin concentration and red cell glutathione levels in patients before and after anaesthesia with halothane or enflurane. These results, though unable to explain the mechanism, support the earlier suggestion that glutathione plays little, if any, role in protecting liver against toxic effects of these anaesthetic agents or their metabolites.


2010 ◽  
Vol 79 (9) ◽  
pp. S3-S8 ◽  
Author(s):  
Eva Straková ◽  
Pavel Suchý ◽  
Romana Kábelová ◽  
František Vitula ◽  
Ivan Herzig

The present paper extends the knowledge of selected blood indicators in six species of feathered game such as helmeted guineafowl (Numida meleagris), common pheasant (Phasianus colchicus), Japanese quail (Coturnix coturnix japonica), chukar (Alectoris chucar), grey partridge (Perdix perdix) and wild turkey (Meleagris gallopavo), reared in the Czech Republic and Europe. The red blood cell (RBC) values in individual species of feathered game ranged as follows: the mean RBC counts varied in a range of 2.10 - 2.58 T/l; haematocrit values were in a range of 0.33 - 0.39 1/1; haemoglobin concentration varied in a range of 101.20 - 129.13 g/l; mean corpuscular haemoglobin concentration (MCHC) ranged from 28.63 to 35.44%; mean corpuscular haemoglobin (MCH) varied in a range of 46.15 - 62.07 pg and the mean corpuscular volume (MCV) was 147.66 - 178.20 fl. Except for the total concentration of haemoglobin and the mean corpuscular haemoglobin concentration, no significant differences were found between the mean values of RBC values in individual species of feathered game. However, some differences between species were found in white blood cell (WBC) values, ranging as follows: the total WBC count ranged from 13.55 to 26.90 G/l, basophil granulocytes from 0.011 to 0.110 G/l, eosinophil granulocytes from 0.011 to 0.593 G/l, neutrophil granulocytes from 1.868 to 6.661 G/l, lymphocytes from 10.937 to 20.922 G/l and monocytes from 0.034 to 0.199 G/l. Most values showed significant (P ⪬ 0.05) to highly significant (P ⪬ 0.01) interspecies differences. The main goal of the study was to investigate selected haematological indicators in six major species of feathered game.


2017 ◽  
Vol 9 (3) ◽  
pp. 1691-1695
Author(s):  
Nishchal Dutta ◽  
Shafiqur Rahman ◽  
Shagufta Azmi ◽  
Muneer Ahmad Dar

The present study was conducted for evaluation of haematological parameters in sheep (330) and goat (230) having various lung affections that were slaughtered routinely as a source of food in Jammu region (Gujjar Nagar, Dogra Hall, Bishnah and Nagrota). About 25.75% (85) and 21.30% (49)of lungs examined in sheep and goats respectively were found to have parasitic infestation of Hydatidosis, Myiasis and Mulleriuscapillaris. Furthermore about 31.81% (105) and 29.13% (67) of selected lungs had pathological conditions of pneumonia, abscess and haemorrhage in sheep and goats respectively. Diseased sheep and goats reflected significant decrease in haemoglobin (Hb), packed cell volume (PCV) and erythrocytes (RBC) in parasitic infestation of Myiasis as compared to control while non significant alteration in these parameters was observed in conditions of Hydatidosis, M. capillaris, pneumonia, abscess and haemorrhage. Mean corpuscular volume (MCV) decreased significantly in Myiasis of sheep and haemorrhagic lungs of goats while no significant change was observed in mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) values. Significant increase in leucocytes (WBC), neutrophils, monocytes, eosinophils were observed in both sheep and goats affected with various parasitic and pathological lung disesases with no significant change in basophilic count. Lymphocytic count decreased in all these adversed lung conditions as compared to healthy ones. Parasitic infection though not mixed one especially that of Myiasis comparatively has significant affected on blood parameters. The present study signifies the complementary importance of ante -mortem examination with that of post-mortem inspection in various parasitic and pathological affections of lungs.


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