Advances in molecular breeding techniques for barley: targeted induced local lesions in genomes (TILLING)

Author(s):  
Serena Rosignoli ◽  
◽  
Silvio Salvi ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 108-131
Author(s):  
Melaku Alemu

There are many constraints that severely affect the global agricultural production and productivity which include the ever increasing of population growth, degradation of natural resources, climate changes and emerging pests. Among these factors, biotic constraints or pests are known to cause 25-50% or complete loss of plant production. Accordingly, various plant protection technologies have been deployed with the trend of focusing on the use modern biotechnological tools that are proven to be most effective and mandatory. The review covers a wide array of pest management methods ranging from the conventional biological control methods up to molecular breeding techniques. Furthermore, the application of new genetic engineering techniques fueled by new breakthroughs and innovations are the cornerstone of this review. Accordingly, the continuous increasing trend of GM crops cultivation in both crop type and hectare has urged many countries to deploy the technology as a key strategy to enhance their bioeconomy. In fact, plant protection is the discipline that immensely benefit from biotechnology than any other disciplines for ensuring food security and sustainable development. However, in order to fully exploit the enormous potential of biotechnology, appropriate biosafety regulatory frameworks and proper stewardship programs need to be effectively implemented. This integrated approach can promptly help respond to the ever-dynamic threat of pests and hence reliably combat food insecurity and ably contribute to sustainable development. Int. J. Appl. Sci. Biotechnol. Vol 8(2): 108-131


2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Per Sikora ◽  
Aakash Chawade ◽  
Mikael Larsson ◽  
Johanna Olsson ◽  
Olof Olsson

Plant mutagenesis is rapidly coming of age in the aftermath of recent developments in high-resolution molecular and biochemical techniques. By combining the high variation of mutagenised populations with novel screening methods, traits that are almost impossible to identify by conventional breeding are now being developed and characterised at the molecular level. This paper provides a comprehensive overview of the various techniques and workflows available to researchers today in the field of molecular breeding, and how these tools complement the ones already used in traditional breeding. Both genetic (Targeting Induced Local Lesions in Genomes; TILLING) and phenotypic screens are evaluated. Finally, different ways of bridging the gap between genotype and phenotype are discussed.


Author(s):  
Nidhi Chakma ◽  
Moutoshi Chakraborty ◽  
Salma Bhyan ◽  
Mobashwer Alam

Abstract This chapter discusses current progress and prospects of molecular breeding and strategies for developing better saline-tolerant sorghum (Sorghum bicolor) varieties. Most molecular breeding techniques for salt tolerance have been carried out in controlled environments where the plants were not exposed to any variation of the surrounding environment, producing reliable results. Due to the polygenic nature of salt tolerance, the identified quantitative trait loci (QTLs) could be false QTLs. Therefore, QTL validation is important in different plant populations and field conditions. Subsequently, marker validation is important before utilizing marker-assisted selection for screening salt-tolerant plants. Combining molecular breeding with conventional breeding can hasten the development of salt-tolerant sorghum varieties.


2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Maria Lobato-Gómez ◽  
Seanna Hewitt ◽  
Teresa Capell ◽  
Paul Christou ◽  
Amit Dhingra ◽  
...  

AbstractBreeding has been used successfully for many years in the fruit industry, giving rise to most of today’s commercial fruit cultivars. More recently, new molecular breeding techniques have addressed some of the constraints of conventional breeding. However, the development and commercial introduction of such novel fruits has been slow and limited with only five genetically engineered fruits currently produced as commercial varieties—virus-resistant papaya and squash were commercialized 25 years ago, whereas insect-resistant eggplant, non-browning apple, and pink-fleshed pineapple have been approved for commercialization within the last 6 years and production continues to increase every year. Advances in molecular genetics, particularly the new wave of genome editing technologies, provide opportunities to develop new fruit cultivars more rapidly. Our review, emphasizes the socioeconomic impact of current commercial fruit cultivars developed by genetic engineering and the potential impact of genome editing on the development of improved cultivars at an accelerated rate.


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