Antimicrobial and <i>in vitro</i> Efficacy of Green Silver Nanoparticles in Tissue Culture of <i>Alhagi maurorum</i>

Abstract The main purpose of the work was to determine the prospects for the use of silver nanoparticles in woody plants tissue culture and to identify possible limitations. The paper presents the results of experiments on the use of silver nanoparticles (AgNPs) to increase the efficiency of in vitro tissue culture of woody plant cultures using the example of gray poplar (Populus × canescens Aiton. Sm.) at various stages of microclonal reproduction. It has been shown that sequential treatment with 7.5 g/L NaOCl for 30 min and 0.3 g/L AgNPs for 5 min allows obtaining 100% phytopathogen-free explants, increasing their viability. Modification of the nutrient medium with AgNPs at concentrations of 1.5…3 μg/L during multiplication and rooting reduces the phytopathogen infestation, and also stimulates the formation of the root system and accelerates the growth of the vegetative part of the shoots. In addition, the combination of photosynthetic and enzymatic activity suggests a rather high potential for stress resistance of experimental microclones in comparison with control ones.

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Effect of Silver Nanoparticles in Stimulating Some Active Compounds in Garlic Callus Under Salt Stress, in Vitro

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/923/1/012023 ◽

2021 ◽

Vol 923 (1) ◽

pp. 012023

Author(s):

Ali Abdulkhudhur Ghalib Al-Taie ◽

Mansoor Abed Aboohanah ◽

Falah Hasan Issa

Keyword(s):

Tissue Culture ◽

Silver Nanoparticles ◽

Salt Stress ◽

Included Study ◽

Active Compounds ◽

Randomized Design ◽

Two Factors ◽

Completely Randomized Design

Abstract This study was conducted in tissue culture laboratory, Faculty of agriculture, University of Kufa in 2020. The experiment included study the effect of two factors: First, different concentrations of Silver nanoparticles(0, 1, and 2 mg.L−1), and second factor : different concentrations of NaCl (0, 25 and 50 mmol.L−1) in Allicin, Di-allyldisulfide and Vinyldithiin content in callus garlic, In vitro. A Completely Randomized Design (C.R.D). Silver nanoparticles showed a significant increase in the active compounds. The concentration (2 mg.L−1) gave highest rate in Allicin, Di-allyldisulfide and Vinyldithiin content (261.36, 529.23 and 309.41μg.g−1 F.W.) respectively. Also, three are significant increased between concentrations of NaCl. The concentration (50 mmol.L−1) gave highest rate in Allicin, Di-allyldisulfide and Vinyldithiin content (250.17, 521.57 and 279.15μg.g−1 F.W.) respectively. While, the interaction tretment (2 mg.L−1 of Silver nanoparticles and 50 mmol.L−1 NaCl) recorded significant increase in Allicin, Di-allyldisulfide and Vinyldithiin content (316.18, 619.06 and 379.39μg.g−1 F.W) respectively.

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RAPID IN VITRO PROPAGATION OF WESTERN GHATS CULTIVAR - COLOCASIA ESCULENTA FOR THE HIGH FREQUENCY OF PLANTLETS

Journal of Drug Discovery and Therapeutics ◽

10.32553/jddt.v7i2.447 ◽

2019 ◽

Vol 7 (2) ◽

Author(s):

Jyothi R ◽

Srinivasa Murthy K M ◽

Hossein . ◽

Veena .

Keyword(s):

Tissue Culture ◽

Wide Distribution ◽

Colocasia Esculenta ◽

Limiting Factor ◽

Promising Alternative ◽

Rapid Multiplication ◽

Medical Plant ◽

Total Fat ◽

Planting Materials

Colocasia esculenta is commonly known as Taro, it is referred to as cocoyam in Nigeria. They are cherished for their rich taste, nutritional and medicinal properties. Every 100 g of taro corms possess 112 Kcal, 26.46 g carbohydrate, 1.50 g protein, 0.20 g total fat and 4.1g fiber (USDA National Nutrient Data Base). Besides its nutritional value, taro is used as a medical plant and provides bioactive compounds used as an anti-cancer drugs. Traditionally, cocoyams are vegetative propagated from tuber fragments, a practice that encourages pathogen distribution. Colocasia esculenta is a widely distributed food crop in the humid tropics and subtropics. Despite of its wide distribution, Taro plants are commonly infected with DsMV and other pathogens. This virus induces conspicuous mosaic, malformation, dwarfing or feathering on leaves in taro. As the results of infection, it reduces the quality and yield of taro production greatly. This virus is thus considered as a major limiting factor in the production of taro. Here plays the importance of tissue culture plays a major role in producing the disease resistant plants round the year with high quality. For rapid multiplication and production of quality planting materials, tissue culture technology offers promising alternative compared to the traditional production methods. KEYWORDS: Colocasia esculenta, Virus, Pathogens, Conventional propagation, Micropropagation, Yield, Rapid multiplication, Quality

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STUDIES ON THE GROWTH OF EXPERIMENTAL OVARIAN TUMOURS IN TISSUE CULTURE

Acta Endocrinologica ◽

10.1530/acta.0.xxxii0041 ◽

1959 ◽

Vol XXXII (I) ◽

pp. 41-53 ◽

Cited By ~ 8

Author(s):

Stig Kullander ◽

Bengt Källén

Keyword(s):

Tissue Culture ◽

Granulosa Cell ◽

In Vitro Study ◽

Vitro Study ◽

Ovarian Tumours

ABSTRACT An in vitro study has been made of experimentally produced rat ovarian tumours of different age, paying particular attention to tumour reaction to crystallized steroids. Tumours of two histological structures were found: granulosa cell – luteoma tumours and arrhenoblastoma tumours. Both types grew in vitro and pictures of their cell appearance are given. The former type gave the best growth, and the endocrine studies were restricted to this type. The steroids tested (androsterone, oestrone, progesterone) all had an arresting effect in certain cases. This effect is not an unspecific, toxic one. The different tumours react to different extents, some being completely unaffected.

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