3’UTR Polymorphism of NRAMP1 Gene and Susceptibility to Lung Tuberculosis among Patients and Nurses in Surabaya, Indonesia

The objectives of this study was to evaluate a potential role for natural-resistance-associated macrophage protein 1 (NRAMP1) gene in the human homologue using four single base pair polymorphisms (D543N, 3’UTR, INT4, 274C/T) for susceptibility to tuberculosis infection in Surabaya, Indonesia. The study population were 69 lung tuberculosis patients and 43 healthy nurses were genotyped with the polymerase chain reaction (PCR) and the product amplified from their genomic DNA were subjected to restriction enzyme digestion (RFLP) and were analysed using agarose gel electrophoresis. Results of this study showed only the homozygous TGTG deletion allele at the 3’untranslated region (3’UTR) of the NRAMP1 gene i.e. the TGTGdel/del genotype was more frequently found in lung tuberculosis patients (20/69=29%) compared to that found in nurses (2/43=4.7%). The Odds ratios (ORs) were 8.37 (95% confidence interval [CI], 1.85 to 37.94; p=0.002). This finding shows that polymorphism 3’UTR of NRAMP1 gene increased the risk of lung tuberculosis in Surabaya, Indonesia.

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Association of Toll –like Receptors 1, 2, 4, 6 ,8, 9 and 10 Genes Polymorphisms and Susceptibility to Tuberculosis in Sudanese Patients

10.21203/rs.3.rs-850575/v1 ◽

2021 ◽

Author(s):

Najwa A Mhmoud

Keyword(s):

Complex Disease ◽

Toll Like Receptors ◽

Tuberculosis Patients ◽

Wide Range ◽

Pcr Rflp ◽

Genes Encoding ◽

Study Population ◽

Polymerase Chain ◽

Tuberculosis Disease

Abstract Background: Genetic factors are important contributors to the development of a wide range of complex disease. Polymorphisms in genes encoding for toll like receptors (TLRs) usually influence the efficiency of the immune response to infection and are associated with disease susceptibility and progression. Therefore, we aims to describe the first association between TLR1, TLR2, TLR4 TLR6 , TLR8 , TLR9 ,and TLR10 genes polymorphisms and susceptibility to tuberculosis in Sudanese patients.Methodology: Here we performed a case study which included 160 tuberculosis patients and 220 healthy matched controls from Sudan. In the study population, we evaluated the possible association between 86 markers in TLR1, TLR2, TLR4 TLR6 , TLR8 , TLR9 ,and TLR10 genes polymorphisms and susceptibility to tuberculosis disease in Sudanese population using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) . Result: From our results It appeared that in the tuberculosis population the TLR1 rs5743557, TLR1 rs4833095, TLR1 rs5743596, TLR2 rs5743704, TLR2 rs5743708, TLR2 rs3804099 , TLR4 rs4986790 ,TLR4 rs4986791, TLR6 rs5743810 , TLR8 rs3764879 , TLR8 rs3764880, TLR9 rs352165, TLR9 rs352167, TLR9 rs187084 and TLR10 rs4129009 were significantly more often encountered (p<0.0001) than in the control population and were associated with tuberculosis in the Sudanese population. For the other polymorphisms tested, no association with tuberculosis was found in the population tested. Conclusion: This indicates that the genotypes obtained for TLR1 rs5743557, TLR1 rs4833095, TLR1 rs5743596, TLR2 rs5743704, TLR2 rs5743708, TLR2 rs3804099 , TLR4 rs4986790 ,TLR4 rs4986791, TLR6 rs5743810 , TLR8 rs3764879 , TLR8 rs3764880, TLR9 rs352165, TLR9 rs352167, TLR9 rs187084 and TLR10 rs4129009 allele have a significant role in the genetic susceptibility to development tuberculosis in Sudanese population.

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Functional analysis of bovine Nramp1 and production of transgenic cloned embryosin vitro

Zygote ◽

10.1017/s096719941300021x ◽

2013 ◽

Vol 23 (1) ◽

pp. 83-92 ◽

Cited By ~ 1

Author(s):

Xiang Cheng ◽

Xiaoli Yu ◽

Yajun Liu ◽

Jie Deng ◽

Xiaoling Ma ◽

...

Keyword(s):

Quantitative Polymerase Chain Reaction ◽

Formation Rate ◽

Natural Resistance ◽

Intracellular Bacteria ◽

Intracellular Pathogens ◽

Qinchuan Cattle ◽

Polymerase Chain ◽

Macrophage Protein ◽

Rectal Palpation

SummaryNatural resistance-associated macrophage protein 1 (Nramp1) plays an important role in restraining the growth of intracellular pathogens within macrophages. In this study,Nramp1cDNA was cloned from Qinchuan cattle and its anti-bacterial activity was demonstrated as being able to significantly inhibit the growth ofSalmonella abortusovisandBrucella abortusin macrophages. Calf fibroblasts stably transfected with pSP–NRAMP1–HA vector were used to reconstruct bovine embryos by somatic cell nuclear transfer (SCNT). Reconstructed embryos were maturatedin vitroand the blastocyst formation rate (14.0%) was similar to that of control embryos (14.5%). Transgenic blastocysts were transplanted into 43 recipient cattle, of which 14 recipients became pregnant as evidenced by non-return estrus and by rectal palpation. One fetus was aborted after 6½ months of pregnancy and transgene integration was confirmed by semi-quantitative polymerase chain reaction. Together, this study showed that bovine Nramp1 retains biological function against the growth of intracellular bacteria and can be used to reconstruct embryos and produceNramp1transgenic cattle, which may benefit the animal and enhance their ability to prevent attack by intracellular pathogens.

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The 14α-Demethylasse(CYP51A1) Gene is Overexpressed in Venturia inaequalis Strains Resistant to Myclobutanil

Phytopathology ◽

10.1094/phyto.2001.91.1.102 ◽

2001 ◽

Vol 91 (1) ◽

pp. 102-110 ◽

Cited By ~ 127

Author(s):

Guido Schnabel ◽

Alan L. Jones

Keyword(s):

Potential Role ◽

Venturia Inaequalis ◽

Target Site ◽

Pcr Analysis ◽

History Of ◽

Allele Specific ◽

Dmi Fungicides ◽

Single Base Pair ◽

Resistant Strains ◽

Polymerase Chain

We identified the cytochrome P450 sterol 14α—demethylase (CYP51A1) gene from Venturia inaequalis and optional insertions located upstream from CYP51A1 and evaluated their potential role in conferring resistance to the sterol demethylation-inhibitor (DMI) fungicide my-clobutanil. The CYP51A1 gene was completely sequenced from one my-clobutanil sensitive (S) and two myclobutanil-resistant (R) strains. No nucleotide variation was found when the three sequences were aligned. Allele-specific polymerase chain reaction (PCR) analysis indicated that a previously described single base pair mutation that correlated with resistance to DMI fungicides in strains of other filamentous fungi was absent in 19 S and 32 R strains of V. inaequalis from Michigan and elsewhere. The sequencing results and PCR analyses suggest that resistance in these strains was not due to a mutation in the sterol demethylase target site for DMI fungicides. Expression of CYP51A1 was determined for strains from an orchard that had never been sprayed with DMI fungicides (baseline orchard), and the data provided a reference for evaluating the expression of strains collected from a research orchard and from three commercial Michigan apple orchards with a long history of DMI use and a high frequency of R strains. Overexpression of CYP51A1 was significantly higher in 9 of 11 R strains from the research orchard than in S strains from the baseline orchard. The high expression was correlated with the presence of a 553-bp insertion located upstream of CYP51A1. Overexpression of the CYP51A1 gene was also detected in eight of eight, five of nine, and nine of nine R strains from three commercial orchards, but the insertion was not detected in the majority of these strains. The results suggest that overexpression of the target-site CYP51A1 gene is an important mechanism of resistance in some field resistant strains of V. inaequalis, but other mechanisms of resistance also appear to exist.

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Mapping of the natural resistance-associated macrophage protein 1 (NRAMP1) gene to pig chromosome 15

Animal Genetics ◽

10.1046/j.1365-2052.1998.00298.x ◽

1998 ◽

Vol 29 (2) ◽

pp. 138-140 ◽

Cited By ~ 11

Author(s):

H. S. Sun ◽

L. Wang ◽

M. F. Rothschild ◽

C. K. Tuggle

Keyword(s):

Natural Resistance ◽

Chromosome 15 ◽

Nramp1 Gene ◽

Macrophage Protein

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Polymorphism of Nramp1 Gene and Its Association with Diarrhea in Pigs

Indian Journal of Animal Research ◽

10.18805/ijar.b-1214 ◽

2020 ◽

Author(s):

Lang Chen ◽

Shuai Peng ◽

Bao-Qiang Fu ◽

Qian Du ◽

Li Zhang ◽

...

Keyword(s):

Disease Resistance ◽

Resistance Breeding ◽

Natural Resistance ◽

Large White ◽

Chi Square ◽

Exon 2 ◽

Pig Breeds ◽

Chi Square Test ◽

Nramp1 Gene ◽

Macrophage Protein

Background: Natural resistance associated macrophage protein 1 (Nramp1) is a relatively conservative gene that plays a crucial role in swine immune response and disease resistance. Methods: We identified the polymorphisms and gene variations in the exon 2 of Nramp1 using polymerase chain reaction–restriction fragment length polymorphism and investigated the correlation between the polymorphisms and piglet diarrhea in four pig breeds (Bamei, Duroc, Landrace, and Large White pigs). Result: The results showed that two alleles (A and B) were identified in all the pig breeds, three genotypes (AA, BB, and AB) were detected in Bamei and Large White breeds, and two genotypes (AA and AB) were detected in Landrace and Duroc breeds. Allele A and genotype AB were dominant in Bamei, Large White, and Landrace breeds, whereas genotype AA was dominant in Duroc pigs. A moderate polymorphism was observed in Landrace and Large White pigs, and polymorphism was abundant in Bamei pigs and low in Duroc pigs. The Chi-square test for Hardy–Weinberg equilibrium disclosed that the exon 2 of Nramp1 in the four breeds of pigs did not deviate from the Hardy–Weinberg balance (P less than 0.05). The results of association analysis showed a significant correlation between breed and piglet diarrhea (P less than 0.05), and the diarrhea score of Bamei pigs was much lower than those of the other breeds. The study could supply theoretical references for further functional research on Nramp1 gene and for screening genes related to disease-resistance breeding.

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