Polymorphism of Nramp1 Gene and Its Association with Diarrhea in Pigs

2020 ◽  
Author(s):  
Lang Chen ◽  
Shuai Peng ◽  
Bao-Qiang Fu ◽  
Qian Du ◽  
Li Zhang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Breeding ◽  
Natural Resistance ◽  
Large White ◽  
Chi Square ◽  
Exon 2 ◽  
Pig Breeds ◽  
Chi Square Test ◽  
Nramp1 Gene ◽  
Macrophage Protein

Background: Natural resistance associated macrophage protein 1 (Nramp1) is a relatively conservative gene that plays a crucial role in swine immune response and disease resistance. Methods: We identified the polymorphisms and gene variations in the exon 2 of Nramp1 using polymerase chain reaction–restriction fragment length polymorphism and investigated the correlation between the polymorphisms and piglet diarrhea in four pig breeds (Bamei, Duroc, Landrace, and Large White pigs). Result: The results showed that two alleles (A and B) were identified in all the pig breeds, three genotypes (AA, BB, and AB) were detected in Bamei and Large White breeds, and two genotypes (AA and AB) were detected in Landrace and Duroc breeds. Allele A and genotype AB were dominant in Bamei, Large White, and Landrace breeds, whereas genotype AA was dominant in Duroc pigs. A moderate polymorphism was observed in Landrace and Large White pigs, and polymorphism was abundant in Bamei pigs and low in Duroc pigs. The Chi-square test for Hardy–Weinberg equilibrium disclosed that the exon 2 of Nramp1 in the four breeds of pigs did not deviate from the Hardy–Weinberg balance (P less than 0.05). The results of association analysis showed a significant correlation between breed and piglet diarrhea (P less than 0.05), and the diarrhea score of Bamei pigs was much lower than those of the other breeds. The study could supply theoretical references for further functional research on Nramp1 gene and for screening genes related to disease-resistance breeding.

Nucleotide variants of the Nramp1 gene in pigs and their effects on disease resistance

2017 ◽  
Author(s):  
Yuliang Wen ◽  
Xiao Cheng ◽  
Shengguo Zhao
Keyword(s):  
Disease Resistance ◽  
Tertiary Structure ◽  
Transmembrane Domains ◽  
Serine Phosphorylation ◽  
Phosphorylation Sites ◽  
Coding Region ◽  
Large White ◽  
Pig Breeds ◽  
Tibetan Pig ◽  
Nramp1 Gene

The Nramp1 gene is an important factor associated with susceptibility to infectious diseases caused by intracellular pathogens. The objective of this study was to determine the structures and sequences of Nramp1 in Tibetan, Gansu Black, Large White, Yorkshire, and Duroc pigs. Sequence analysis of the Nramp1 gene in the five pig breeds revealed the presence of 11 nucleotide variants in intronic regions, 2 nucleotide variants in the control region, 10 nucleotide variants and one deletion in the 3’ non-coding region, and 15 nucleotide variants in the exons. However, only 4 nucleotide variants resulted in amino acid changes. Further analysis predicted that the Tibetan pig Nramp1 protein harbors 10 transmembrane domains. The analysis also predicted 10 serine phosphorylation sites, 3 threonine phosphorylation sites, and 4 tyrosine phosphorylation sites in the Nramp1 protein of Tibetan pigs. Predictions of the Tibetan pig Nramp1 tertiary structure revealed 7 putative a -helices and 5 putative b-sheets. Predictions of the Yorkshire pig Nramp1 protein revealed significant differences compared with the Tibetan pig Nramp1 protein. The results indicated that differences in transmembrane domains and tertiary structures of the Nramp1 protein of the Tibetan and Yorkshire pig breeds could explain differences in the disease resistance of these two breeds

145 Effect of Substandard Teats on Piglet and Sow Performance

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 133-134
Author(s):  
Mark Knauer ◽  
Zack Peppmeier
Keyword(s):  
Mammary Gland ◽  
Birth Weight ◽  
Litter Size ◽  
Mixed Models ◽  
Research Station ◽  
Large White ◽  
Chi Square ◽  
Maternal Line ◽  
Chi Square Test ◽  
Piglet Survival

Abstract The objective of the study was to evaluate sow teat quality in relation to subsequent reproductive throughput. Data included one cohort of 42 Landrace × Large White second parity maternal line sows at the Tidewater Research Station (Plymouth, NC). Functional teats (FUNCTIONAL) were classified pre-farrow as acceptable (ACCEPTABLE) or substandard (SUBSTANDARD). Teats were categorized as SUBSTANDARD when teat size was ≤75% of ACCEPTABLE. At day 20 of lactation, ACCEPTABLE and SUBSTANDARD were assessed for the presence of a swollen mammary gland and piglets observed nursing SUBSTANDARD were recorded (24 piglets from 15 litters). Means for FUNCTIONAL, ACCEPTABLE and SUBSTANDARD were 15.07, 13.59 and 1.48 teats, respectively. Biological dam traits included birth weight (BWT), total number born (TNB), litter size at weaning (LSW) and piglet survival (LSW/TNB). Weaning weight (WWT) was considered a trait of the nurse dam. Means for TNB, LSW, litter BWT and litter WWT were 13.4, 10.9, 17.8 kg and 57.8 kg, respectively. Data was analyzed using a chi-square test for binary traits and linear mixed models for continuous traits. At weaning, a greater (P < 0.01) proportion of ACCEPTABLE had a functional mammary gland when compared to SUBSTANDARD (76 vs. 47%). Within litters, piglets nursing SUBSTANDARD tended (em>P = 0.09) to be 158 grams lighter at weaning in comparison to ACCEPTABLE. Yet BWT of SUBSTANDARD piglets did not differ (em>P = 0.60) from piglets nursing ACCEPTABLE. Across litters, an increase in one SUBSTANDARD tended (em>P = 0.07) to reduce litter WWT by 1.74 kilograms. An increase in one SUBSTANDARD tended (em>P < 0.07) to increase piglet survival by 3.5%. Results suggest substandard teats, identified pre-farrow, are less likely to have a functional mammary gland at weaning, impair piglet quality yet may enhance piglet survival.

scholarly journals Study on genetic variability in MHC-DRB1 second exon in Makuie sheep breed population

Genetika ◽  
2014 ◽  
Vol 46 (1) ◽  
pp. 269-275 ◽  
Author(s):  
Fereshteh Ashrafi ◽  
Ali Hashemi ◽  
Karim Mardani ◽  
Reza Darvishzadeh
Keyword(s):  
Major Histocompatibility Complex Gene ◽  
Enzymatic Digestion ◽  
Sheep Breed ◽  
Chi Square ◽  
Exon 2 ◽  
Sheep Population ◽  
Histocompatibility Complex ◽  
Pcr Products ◽  
Chi Square Test ◽  
Hardy Weinberg Equilibrium

In the present study polymorphism of the exon 2 of MHC (Major Histocompatibility Complex) gene in Makuie sheep breed was studied. Genomic DNA from blood samples of 90 sheep was extracted and a 279 bp MHC exon 2 fragment was amplified using polymerase chain reaction (PCR). PCR products were subjected to enzymatic digestion using RsaI endonuclease. Digested PCR products were electrophoresed on 2% agarose gel. The results showed the existence of 10 alleles: A, B, E, F, I, M, O, P, Q and V for the exon 2 of the MHC gene, with the frequencies of 0.4756, 0.0976, 0.0183, 0.0366, 0.0549, 0.0122, 0.1098, 0.0915, 0.0854 and 0.0183, respectively. Eighteen genotypes: AA, AB, AE, FF, AM, BO, EO, IO, OM, AP, BP, OP, PP, AQ, OQ, PQ, QQ and AV with the frequencies of 0.317, 0.1585, 0.0121, 0.0365, 0.0121, 0.0243, 0.0243, 0.1097, 0.0121, 0.0487, 0.0121, 0.0365, 0.0365, 0.0487, 0.0121, 0.0121, 0.0487 and 0.0365, respectively were identified in the population under study. Effective number of alleles and heterozygosity for the examined region were 3.7231 and 0.7314, respectively. Chi-square test showed that the examined sheep population was not in Hardy-Weinberg equilibrium in the examined region. <br><br><font color="red"><b> This article has been corrected. Link to the correction <u><a href="http://dx.doi.org/10.2298/GENRSR1602800A">10.2298/GENRSR1602800A</a><u></b></font>

scholarly journals Survival Analysis of Hungarian Large White, Duroc and Pietrain Sows

2016 ◽  
pp. 31-36
Author(s):  
Ágnes Baginé Hunyadi ◽  
Szilvia Kusza ◽  
Péter Balogh
Keyword(s):  
Survival Analysis ◽  
Rank Test ◽  
Large White ◽  
Chi Square ◽  
Survival Percentage ◽  
Kaplan Meier ◽  
Significant Difference ◽  
Chi Square Test ◽  
Single Factor Analysis ◽  
The Individual

The aim of the present study was to perform lifetime performance analysis in three pig breeds; Hungarian Large White (n=295), Duroc (n=76) and Pietrain (n=91) on a commercial farm using analysis of survival sows. We took into consideration the age of sows at the time of their inclusion into breeding, their age at the time of culling, time spent in production, number of mating and parities, parity percentage, intervals between litters, number and mean of piglets born alive and born dead, number of raised piglet litters, number and mean of 21 days old piglets, the weight and mean of raised litter and raise percentage. We carried out the analysis by SPSS 22.0. Single factor analysis of variants, Kaplan-Meier analysis and Cox PH model were used. The determination of the significance of risk rates differences was done by Wald chi square test. Our results showed that the average culling age were 1056 (±33.52) days for the Hungarian Large White, 735 (±73.56) days for Duroc and 818 (±71.98) days for the Pietrain. The log rank test of the survival analysis indicated a significant difference between the three tested genotypes (χ2=16.981, P<0.001), which means that the survival percentage of the individual breeds varied significantly from one another. In comparison with the Hungarian Large White genotype the Duroc genotype has a 1.6 times higher (P<0.001) culling risk while that of the genotype Pietrain was 1.36 times higher (P<0.001). Our results can be used to compare the breeds kept under the same conditions and to compare the life span of one genotype under different farming conditions. Factors that increase survival and improve the profitability of pig farming can be determined by this method.

Mapping of the natural resistance-associated macrophage protein 1 (NRAMP1) gene to pig chromosome 15

1998 ◽  
Vol 29 (2) ◽  
pp. 138-140 ◽  
Author(s):  
H. S. Sun ◽  
L. Wang ◽  
M. F. Rothschild ◽  
C. K. Tuggle
Keyword(s):  
Natural Resistance ◽  
Chromosome 15 ◽  
Nramp1 Gene ◽  
Macrophage Protein

scholarly journals Role of SLC11A1 gene in disease resistance

2012 ◽  
Vol 28 (1) ◽  
pp. 99-106 ◽  
Author(s):  
N. Thomas ◽  
S. Joseph
Keyword(s):  
Disease Resistance ◽  
Genetic Resistance ◽  
Integral Membrane Protein ◽  
Natural Resistance ◽  
Human Beings ◽  
Economic Traits ◽  
Slc11a1 Gene ◽  
Breeding Programmes ◽  
Macrophage Protein

Genetic improvement in livestock was achieved earlier by selective breeding of individuals with superior phenotypes. Now due to the advances in molecular genetics and biotechnology candidate genes of economic traits can be included in selection for breeding programmes. Genes responsible for the resistance/susceptibility to infections with various pathogens (Major Histo Compatibility (MHC) genes, Solute Carrier family11 member A1 (SLC11A1) gene, Toll Like Receptor (TLR) genes etc.), have been recently identified and characterized in human beings as well as in many animals. Among these the role of SLC11A1 gene is very important due to its association with resistance/ susceptibility to various intracellular pathogens in human as well as in livestock species. The SLC11A1 gene, formerly known as natural resistance-associated macrophage protein 1 (NRAMP1) encodes an integral membrane protein regulating the activity of macrophages. Genetic resistance/ susceptibility to diseases due to candidate gene polymorphisms could be used in selection and breeding for disease resistance in animals.

scholarly journals Influence of the reproductive cycle on the zootechnical indexes in hyperproliferous swine matrices

2021 ◽  
Vol 10 (2) ◽  
pp. e14610212367
Author(s):  
Idael Matheus Góes Lopes ◽  
João Paulo Pereira de Souza ◽  
Marcelo Dourado de Lima ◽  
Kariny Fonseca da Silva ◽  
Marley Conceição dos Santos ◽  
...  
Keyword(s):  
Reproductive Efficiency ◽  
Large White ◽  
Chi Square ◽  
Live Births ◽  
The Third ◽  
Tukey Test ◽  
Chi Square Test ◽  
Ad Libitum ◽  
Reproductive Cycles ◽  
Number Of Births

In swine breeding stock, the calving order has a direct correlation with the performance of the litter, both at birth and at weaning. Thus, the present study aimed to evaluate the effect of the number of reproductive cycles of swine females on their zootechnical indexes. Forty healthy swine females from an industrial cross (Landrace X Large White) were used, distributed in five treatments, eight females per treatment, submitted to the same type of diet and water ad libitum. Being carried out the management of confirmation of pregnancies or return to heat and transfer to maternity After calving, productive indexes of females and piglets were evaluated. The data obtained were analyzed through analysis of variance, and comparison of means by the Tukey test (p <5%). In alternative cases, the chi-square test was performed. There was an increase in the number of piglets born total and live births up to the third cycle, followed by a drop in the fourth and fifth cycle. There was no difference between treatments in relation to piglet weight at birth, weaning and mummified rate. However, there was a relationship between the number of births, the rate of stillbirths, deaths from crushing and repetition of heat, showing a reduction in such rates due to the advancing age of the mothers. Thus, the results demonstrated that the total number of piglets born and live births increases while losses due to stillbirth, crushing and repetition of heat tend to decrease with advancing age of the females, with greater reproductive efficiency achieved between the third and fourth cycle.

scholarly journals 3’UTR Polymorphism of NRAMP1 Gene and Susceptibility to Lung Tuberculosis among Patients and Nurses in Surabaya, Indonesia

2010 ◽  
Vol 1 (1) ◽  
pp. 17
Author(s):  
Rahayu Anggraini
Keyword(s):  
Potential Role ◽  
Natural Resistance ◽  
Restriction Enzyme Digestion ◽  
Tuberculosis Patients ◽  
Lung Tuberculosis ◽  
Study Population ◽  
Nramp1 Gene ◽  
Single Base Pair ◽  
Polymerase Chain ◽  
Macrophage Protein

The objectives of this study was to evaluate a potential role for natural-resistance-associated macrophage protein 1 (NRAMP1) gene in the human homologue using four single base pair polymorphisms (D543N, 3’UTR, INT4, 274C/T) for susceptibility to tuberculosis infection in Surabaya, Indonesia. The study population were 69 lung tuberculosis patients and 43 healthy nurses were genotyped with the polymerase chain reaction (PCR) and the product amplified from their genomic DNA were subjected to restriction enzyme digestion (RFLP) and were analysed using agarose gel electrophoresis. Results of this study showed only the homozygous TGTG deletion allele at the 3’untranslated region (3’UTR) of the NRAMP1 gene i.e. the TGTGdel/del genotype was more frequently found in lung tuberculosis patients (20/69=29%) compared to that found in nurses (2/43=4.7%). The Odds ratios (ORs) were 8.37 (95% confidence interval [CI], 1.85 to 37.94; p=0.002). This finding shows that polymorphism 3’UTR of NRAMP1 gene increased the risk of lung tuberculosis in Surabaya, Indonesia.

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