scholarly journals ANTIMICROBIAL ACTIVITY OF PINEAPPLE (ANANAS COMOSUS L. MERR) EXTRACT AGAINST MULTIDRUG-RESISTANT OF PSEUDOMONAS AERUGINOSA: AN IN VITRO STUDY

2017 ◽  
Vol 6 (5) ◽  
pp. 118 ◽  
Author(s):  
Rahmat Sayyid Zharfan ◽  
Priyo Budi Purwono ◽  
Arifa Mustika
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Agar Plate ◽  
Multidrug Resistant ◽  
Ananas Comosus ◽  
Tract Infections

Pseudomonas aeruginosa is the main cause of nosocomial infection which is responsible for 10% of hospital-acquired infection. Pseudomonas aeruginosa tends to mutate and displays potential for development of antibiotic resistance. Approximately, 10% of global bacterial isolates are found as Multidrug-resistant Pseudomonas aeruginosa. Pseudomonas aeruginosa have a quite tremendous severity index, especially on pneumonia and urinary tract infections, even sepsis, which 50% mortality rate. Pineapple (Ananas comosus L. Merr) has antimicrobial properties. The active antimicrobial compounds in Ananas comosus L. Merr include saponin and bromelain. This research aims to find the potency of antimicrobial effect of pineapple (Ananas comosus L. Merr) extract towards Multidrug-resistant Pseudomonas aeruginosa. Multidrug-resistant Pseudomonas aeruginosa specimen is obtained from patient’s pus in orthopaedic department, Dr Soetomo Public Hospital, Surabaya. Multidrug-resistant Pseudomonas aeruginosa specimen is resistant to all antibiotic agents except cefoperazone-sulbactam. This research is conducted by measuring the Minimum Inhibitory Concentration (MIC) through dilution test with Mueller-Hinton broth medium. Pineapple extract (Ananas comosus L. Merr.) is dissolved in aquadest, then poured into test tube at varying concentrations (6 g/ml; 3 g/ml; 1.5 g/ml; 0.75 g/ml, 0.375 g/ml; and 0.1875 g/ml). After 24 hours’ incubation, samples are plated onto nutrient agar plate, to determine the Minimum Bactericidal Concentration (MBC). The extract of pineapple (Ananas comosus L. Merr) has antimicrobial activities against Multidrug-resistant Pseudomonas aeruginosa. Minimum Inhibitory Concentration (MIC) could not be determined, because turbidity changes were not seen. The Minimum Bactericidal Concentration (MBC) of pineapple extract (Ananas comosus L. Merr) to Multidrug-resistant Pseudomonas aeruginosa is 0.75 g/ml. Further study of in vivo is needed.

scholarly journals Antibacterial and Hemolytic Activity of Crotalus triseriatus and Crotalus ravus Venom

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 281
Author(s):  
Adrian Zaragoza-Bastida ◽  
Saudy Consepcion Flores-Aguilar ◽  
Liliana Mireya Aguilar-Castro ◽  
Ana Lizet Morales-Ubaldo ◽  
Benjamín Valladares-Carranza ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Hemolytic Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
The Other ◽  
Medical Treatments ◽  
First Time

Rattlesnakes have venoms with a complex toxin mixture comprised of polypeptides and proteins. Previous studies have shown that some of these polypeptides are of high value for the development of new medical treatments. The aim of the present study is to evaluate, in vitro, the antibacterial and hemolytic activity of Crotalus triseriatus and Crotalus ravus venoms. A direct field search was conducted to obtain Crotalus triseriatus and Crotalus ravus venom samples. These were evaluated to determine their antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa through the techniques of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Hemolytic activity was also determined. Antibacterial activity was determined for treatments (Crotalus triseriatus 2) CT2 and (Crotalus ravus 3) CR3, obtaining a Minimum Inhibitory Concentration of 50 µg/mL and a Minimum Bactericidal Concentration of 100 µg/mL against Pseudomonas aeruginosa. CT1 (Crotalus triseriatus 1), CT2, and CR3 presented hemolytic activity; on the other hand, Crotalus ravus 4 (CR4) did not show hemolytic activity. The results of the present study indicate for the first time that Crotalus triseriatus and Crotalus ravus venoms contain some bioactive compounds with bactericidal activity against Pseudomonas aeruginosa which could be used as alternative treatment in diseases caused by this pathogenic bacterium.

scholarly journals Anti-Shigellosis Activity of Cola anomala Water/Ethanol Pods Extract on Shigella flexneri-Induced Diarrhea in Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Henri Wambe ◽  
Paul Aimé Noubissi ◽  
Michel Archange Fokam Tagne ◽  
Angèle Foyet Fondjo ◽  
Gaëtan Olivier Fankem ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Shigella Flexneri ◽  
White Blood Cells ◽  
Diffusion Method ◽  
Albino Rats ◽  
Disk Diffusion Method

This study was undertaken to evaluate the activities of water/ethanol Cola anomala pods extract. In vitro antimicrobial susceptibility was determined by the disk diffusion method; the minimum inhibitory concentration and minimum bactericidal concentration were determined by agar dilution technique. In vivo, shigellosis was induced in healthy Wistar albino rats by oral administration of Shigella flexneri inoculum, 12 × 108 CFU/mL. At the onset of diarrhea, infected and normal control animals were subdivided into various groups treated with distilled water, with water/ethanol Cola anomala pods extract at 25, 50, or 100 mg/kg, or with ciprofloxacin, 2.5 mg/kg. After one-week treatment, rats were sacrificed, and blood and colon were collected. Blood was used for blood cell count. A portion of the colon served for histological studies while homogenate from the remaining part was centrifuged and the supernatant was collected for the determination of NO, PGE2, IL-1β, and TNF-α levels. In vitro, water/ethanol Cola anomala pods extract showed to be bactericidal, with a minimum inhibitory concentration of 2.0 mg/mL and a minimum bactericidal concentration of 3.0 mg/mL. In diarrheic rats, the extract significantly (P<0.01) increased the white blood cells and significantly (P<0.01) decreased stool Shigella density from the first to the seventh day of treatment. It partially restored the structure of eroded intestine epithelium and prevented weight loss; the dose dependently and significantly (P<0.001) decreased NO, IL-1β, and TNF-α production in the colon and was found to have no significant effect on PGE2 production. These results support the use of this plant in traditional medicine in the treatment of gastrointestinal ailments.

scholarly journals EFEKTIVITAS EKSTRAK ETANOL BIJI KURMA MESIR (Phoenix dactylifera L.) SEBAGAI ANTIBAKTERI TERHADAP Streptococcus pyogenes SECARA IN VITRO

2019 ◽  
Vol 19 (3) ◽  
Author(s):  
Lilia Putri Pratama
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Pyogenes ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Agar Plate ◽  
Ethanol Extract ◽  
Phoenix Dactylifera ◽  
Blood Agar Plate ◽  
Phoenix Dactylifera L

Abstrak. Kurma (Phoenix dactylifera L.) telah dikonsumsi secara luas di seluruh duniadan diketahui memiliki banyak manfaat di bidan kesehatan. Salah satu mafaatnya yaitu sebagai obat terhadap faringitis yang disebabkan oleh bakteri Streptococcus pyogenes. Terdapat ±517.000 kematian pertahun yang disebabkan oleh infeksi Streptococcus pyogenes dan komplikasinya. Sejauh ini telah banyak studi mengenai manfaat daging kurma, namun manfaat terhadap biji kurma belum banyak diteliti. Maka dari itu penelitian ini bertujuan untuk menguji efek antibakteri ekstrak etanol biji kurma Mesir (Phoenix dactylifera L.) pada Streptococus pyogenes  secara in vitro dengan uji Konsentrasi Hambat Minimal (KHM) menggunakan metode dilusi serial dan uji Konsentrasi Bunuh Minimal (KBM) dengan inokulasi pada media agar darah. Konsentrasi yang digunakan dalam penelitian ini adalah 1428.57 mg/ml, 714,29 mg/ml, 357.14 mg/ml, 178.57 mg/ml, 89.29 mg/ml, 44,64 mg/ml, 22,32 mg/ml, dan 11,16 mg/ml. Dari penelitian tersebut didapatkan bahwa Konsentrasi Hambat Minimal (KHM) tidak dapat ditentukan dan nilai Konsentrasi Bunuh Minimal (KBM) sebesar 1428.57 mg/ml. Ekstrak etanol biji kurma Mesir (Phoenix dactylifera L.) efektif sebagai antibakteri terhadap Streptococcus pyogenes dengan nilai signifikan 0,002 (p0,05) Kata Kunci: Biji Kurma Mesir, Phoenix dactylifera L., Streptococcus pyogenes, faringitis Abstract. Dates (Phoenix dactylifera L.) are widely consumed in various parts of the world and are known to have many benefits in the health sector. One of them is pharyngitis medication caused by Streptococcus pyogenes. There are ±517.000 deaths per year which caused by Streptococcus pyogenes infection and its complication. So far there have been many studies on the benefits of date flesh, but the benefits of the seeds have not been widely studied. The aim of this study is to test the anti-bacterial effect of the ethanol extract of Egyptian Phoenix dactylifera L. seed on Streptococcus pyogenes in vitro by Minimum Inhibitory Concentration test using serial dilution method and Minimum Bactericidal Concentration (MBC) in blood agar plate inoculates.  The concentration used in this study are 1428.57 mg/ml, 714,29 mg/ml, 357.14 mg/ml, 178.57 mg/ml, 89.29 mg/ml, 44,64 mg/ml, 22,32 mg/ml, and 11,16 mg/ml. From this study the Minimum Inhibitory Concentration (MIC) can not be determined and the Minimum Bactericidal Concentration is 1428.57 mg/ml. Ethanol extract of Egyptian Phoenix dactylifera L. seed effective as antibacteri against Streptococcus pyogenes with a significant value of 0,002 (p 0,05).    Keywords: date palm seeds, Phoenix dactylifera L, Streptococcus pyogenes, pharyngitis. 

scholarly journals Efektivitas ekstrak etanol biji kurma mesir (Phoenix dactylifera L.) sebagai antibakteri terhadap Streptococcus pyogenes secara in vitro

2019 ◽  
Vol 19 (3) ◽  
Author(s):  
Lilia Putri Pratama ◽  
Marijam Purwanta ◽  
Ema Qurnianingsih
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Streptococcus Pyogenes ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Agar Plate ◽  
Ethanol Extract ◽  
Phoenix Dactylifera ◽  
Blood Agar Plate ◽  
Phoenix Dactylifera L

Abstrak. Kurma (Phoenix dactylifera L.) telah dikonsumsi secara luas di seluruh duniadan diketahui memiliki banyak manfaat di bidan kesehatan. Salah satu mafaatnya yaitu sebagai obat terhadap faringitis yang disebabkan oleh bakteri Streptococcus pyogenes. Terdapat ±517.000 kematian pertahun yang disebabkan oleh infeksi Streptococcus pyogenes dan komplikasinya. Sejauh ini telah banyak studi mengenai manfaat daging kurma, namun manfaat terhadap biji kurma belum banyak diteliti. Maka dari itu penelitian ini bertujuan untuk menguji efek antibakteri ekstrak etanol biji kurma Mesir (Phoenix dactylifera L.) pada Streptococus pyogenes  secara in vitro dengan uji Konsentrasi Hambat Minimal (KHM) menggunakan metode dilusi serial dan uji Konsentrasi Bunuh Minimal (KBM) dengan inokulasi pada media agar darah. Konsentrasi yang digunakan dalam penelitian ini adalah 1428.57 mg/ml, 714,29 mg/ml, 357.14 mg/ml, 178.57 mg/ml, 89.29 mg/ml, 44,64 mg/ml, 22,32 mg/ml, dan 11,16 mg/ml. Dari penelitian tersebut didapatkan bahwa Konsentrasi Hambat Minimal (KHM) tidak dapat ditentukan dan nilai Konsentrasi Bunuh Minimal (KBM) sebesar 1428.57 mg/ml. Ekstrak etanol biji kurma Mesir (Phoenix dactylifera L.) efektif sebagai antibakteri terhadap Streptococcus pyogenes dengan nilai signifikan 0,002 (p0,05)Kata Kunci: Biji Kurma Mesir, Phoenix dactylifera L., Streptococcus pyogenes, faringitis Abstract. Dates (Phoenix dactylifera L.) are widely consumed in various parts of the world and are known to have many benefits in the health sector. One of them is pharyngitis medication caused by Streptococcus pyogenes. There are ±517.000 deaths per year which caused by Streptococcus pyogenes infection and its complication. So far there have been many studies on the benefits of date flesh, but the benefits of the seeds have not been widely studied. The aim of this study is to test the anti-bacterial effect of the ethanol extract of Egyptian Phoenix dactylifera L. seed on Streptococcus pyogenes in vitro by Minimum Inhibitory Concentration test using serial dilution method and Minimum Bactericidal Concentration (MBC) in blood agar plate inoculates.  The concentration used in this study are 1428.57 mg/ml, 714,29 mg/ml, 357.14 mg/ml, 178.57 mg/ml, 89.29 mg/ml, 44,64 mg/ml, 22,32 mg/ml, and 11,16 mg/ml. From this study the Minimum Inhibitory Concentration (MIC) can not be determined and the Minimum Bactericidal Concentration is 1428.57 mg/ml. Ethanol extract of Egyptian Phoenix dactylifera L. seed effective as antibacteri against Streptococcus pyogenes with a significant value of 0,002 (p 0,05).Keywords: date palm seeds, Phoenix dactylifera L, Streptococcus pyogenes, pharyngitis.

The in vitro efficacy of neutral electrolysed water and povidone-iodine against CRS-associated biofilms

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
C.A. Lux ◽  
K. Biswas ◽  
M.W. Taylor ◽  
R.G. Douglas
Keyword(s):  
Inhibitory Concentration ◽  
Povidone Iodine ◽  
Minimum Bactericidal Concentration ◽  
Treatment Options ◽  
Antimicrobial Treatment ◽  
Biofilm Reactor ◽  
Bacterial Biofilms ◽  
Antibiofilm Activity

Background: Despite best medical and surgical practice, some cases of chronic rhinosinusitis (CRS) can remain recalcitrant. Bacterial biofilms have been associated with the recalcitrance of sinonasal inflammation. Biofilms are highly resistant to commonly prescribed antibiotics. Accordingly, more effective antimicrobial treatment options are needed to treat refractory CRS. The aim of this study was to determine the in vitro efficacy of neutral electrolysed water (NEW) and povidone-iodine (PVI) against CRS-associated Staphylococcus aureus biofilms. Methods: Mature S. aureus biofilms were grown in a Centre for Disease Control (CDC) biofilm reactor. The antimicrobial activity of NEW, PVI and doxycycline was determined for both planktonic and biofilm cultures of a clinical S. aureus isolate using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum biofilm eradication concentration (MBEC) assays. Results: MICs and MBCs were determined for all antimicrobials. MBC values were similar to MICs for both antiseptics, but doxycycline MBCs were significantly higher than the associated MICs. Biofilms were highly resistant to NEW and doxycycline. The MBEC for doxycycline was between 500 and 1000 µg/mL. NEW was ineffective against biofilms and no MBEC could be determined. In contrast, a concentration of 10% of the commercial PVI solution (10 mg/mL PVI) led to effective eradication of mature biofilms. Conclusion: In this study, only PVI showed promising antibiofilm activity at physiological concentrations. The in vivo efficacy of PVI warrants further investigation of its potential as a treatment for recalcitrant CRS.

scholarly journals THE EFFECT OF PROPOLIS EXTRACT AND PROPOLIS CANDIES ON THE GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC 43718

2017 ◽  
Vol 10 (17) ◽  
pp. 26
Author(s):  
Khodijah Khodijah ◽  
Ratna Farida ◽  
Nurtami Soedarsono
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Spectrophotometric Analysis ◽  
Propolis Extract ◽  
Post Exposure ◽  
Colony Forming Units

Objective: This experiment aimed to analyze the effect of propolis extract and propolis containing candies on the growth of Aggregatibacter actinomycetemcomitans using spectrophotometric analysis and colony-forming units (CFU) counts.Methods: After A. actinomycetemcomitans were exposed to propolis extract and candies, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined with spectrophotometry and post-exposure colony counting.Results: The MIC of propolis extract against A. actinomycetemcomitans was determined to be 10%, and the MBC was 20%. A decrease in the total CFU count of A. actinomycetemcomitans was observed after propolis extract and candy exposure.Conclusions: Propolis extract and propolis candies were effective in inhibiting the growth of A. actinomycetemcomitans ATCC 43718 in vitro.

scholarly journals Physicochemical and antimicrobial assessment of Iranian Propolis gathered in Qazvin province

2020 ◽  
Vol 10 (2) ◽  
pp. 82
Author(s):  
Fatemeh Samieerad ◽  
Nematollah Gheibi
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Minimum Inhibitory Concentration ◽  
Thin Layer ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antibacterial Effect ◽  
Ethanol Extract ◽  
Standard Strain ◽  
Layer Chromatography

Background: Propolis is one of the useful bee colony products that have been used in traditional medicine for centuries. In this study, the physicochemical characters and their antibacterial effect of Iranian Propolis collected from Qazvin province was assessed.Methods: In this study, Thin Layer Chromatography and Vacuum Liquid Chromatography to detect different compounds of the extract have been used. In the initial evaluation of Propolis extract, it was found that the extract includes variable compounds with different polarity; so, the initial classification of extract with different polarity solvents was essential. Finally, 0.1 gr hydro alcoholic Propolis was injected to the HPLC by ultrasound. The antibacterial effect of Iranian ethanol extract Propolis was measured using a microdilution method against Pseudomonas aeruginosa: P. aeruginosa and Staphylococcus aureus: S.aureus standard strains and the minimum bactericidal and inhibitory concentration were defined.Results: Primary analysis of the ethanol extract by analytical Thin Layer Chromatography, demonstrated the presence of flavonoid and phenol in it. Minimum inhibitory concentration and Minimum Bactericidal Concentration for Staphylococcus aureus: S.aureus standard strain was 2.5mg/ml. The same procedure was done for Pseudomonas aeruginosa: P. aeruginosa standard strain and the Minimum inhibitory concentration and Minimum Bactericidal Concentration were 50mg/ml of Propolis extracts.  Conclusion: According to the results, the alcoholic extract of propolis from Qazvin province of Iran provides significant antimicrobial activity. Its powerful activity may be due to high total phenolic and flavonoid contents.Keywords: Iranian propolis, Antibacterial activity, Phenolic compounds, Flavonoid compound

scholarly journals Pilot Ex Vivo and In Vitro Evaluation of a Novel Foley Catheter with Antimicrobial Periurethral Irrigation for Prevention of Extraluminal Biofilm Colonization Leading to Catheter-Associated Urinary Tract Infections (CAUTIs)

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Nylev Vargas-Cruz ◽  
Joel Rosenblatt ◽  
Ruth A Reitzel ◽  
Anne-Marie Chaftari ◽  
Ray Hachem ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Foley Catheter ◽  
Irrigation Treatment ◽  
Multidrug Resistant ◽  
Indwelling Catheter ◽  
Carbapenem Resistant ◽  
Colonization Model ◽  
Tract Infections

CAUTI remains a serious healthcare issue for incontinent patients whose urine drainage is managed by catheters. A novel double-balloon Foley catheter was developed which was capable of irrigating the extraluminal catheter surfaces within the periurethral space between the urethral-bladder junction and meatus. The catheter has a retention cuff that is inflated to secure the catheter in the bladder and a novel irrigation cuff proximal to the urethral-bladder junction capable of providing periurethral irrigation from the urethral-bladder junction to the meatus. Uniform periurethral irrigation was demonstrated in an ex vivo porcine model by adding a dye to the antimicrobial urethral irrigation solution. An in vitro biofilm colonization model was adapted to study the ability of periurethral irrigation with a newly developed antimicrobial combination consisting of polygalacturonic acid + caprylic acid (PG + CAP) to prevent axial colonization of the extraluminal urethral indwelling catheter shaft by common uropathogens. The extraluminal surface of control catheters that were not irrigated formed biofilms along the entire axial urethral tract after 24 hours. Significant (p<0.001) inhibition of colonization was seen against multidrug-resistant Pseudomonas aeruginosa (PA), carbapenem-resistant Escherichia coli (EC), and carbapenem-resistant Klebsiella pneumoniae (KB). For other common uropathogens including Candida albicans (CA), Proteus mirabilis (PR), and Enterococcus faecalis (EF), a first irrigation treatment completely inhibited colonization of half of the indwelling catheter closest to the bladder and a second treatment largely disinfected the remaining intraurethral portion of the catheter towards the meatus. The novel Foley catheter and PG + CAP antimicrobial irrigant prevented biofilm colonization in an in vitro CAUTI model and merits further testing in an in vivo CAUTI prevention model.

scholarly journals Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using Immunoinformatics

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Mustafa Elhag ◽  
Ruaa Mohamed Alaagib ◽  
Nagla Mohamed Ahmed ◽  
Mustafa Abubaker ◽  
Esraa Musa Haroun ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Peptide Vaccine ◽  
Multidrug Resistant ◽  
Fructose Bisphosphate ◽  
Mhc I ◽  
Mhc Ii ◽  
Fructose Bisphosphate Aldolase ◽  
Hospital Acquired

Pseudomonas aeruginosa is a common pathogen that is responsible for serious hospital-acquired infections, ventilator-associated pneumonia, and various sepsis syndromes. Also, it is a multidrug-resistant pathogen recognized for its ubiquity and its intrinsically advanced antibiotic-resistant mechanisms. It usually affects immunocompromised individuals but can also infect immunocompetent individuals. There is no vaccine against it available till now. This study predicts an effective epitope-based vaccine against fructose bisphosphate aldolase (FBA) of Pseudomonas aeruginosa using immunoinformatics tools. The protein sequences were obtained from NCBI, and prediction tests were undertaken to analyze possible epitopes for B and T cells. Three B cell epitopes passed the antigenicity, accessibility, and hydrophilicity tests. Six MHC I epitopes were found to be promising, while four MHC II epitopes were found promising from the result set. Nineteen epitopes were shared between MHC I and II results. For the population coverage, the epitopes covered 95.62% worldwide excluding certain MHC II alleles. We recommend in vivo and in vitro studies to prove its effectiveness.

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