scholarly journals Cytoxicity test of NaOCl and Mangosteen (Garcinia Mangostin L.) peel extract used as an irrigation solution in human periodontal ligament fibroblast cells (HPdLFc)

2018 ◽  
Vol 51 (3) ◽  
pp. 133
Author(s):  
Tamara Yuanita ◽  
Dina Ristyawati ◽  
Karlina Samadi
Keyword(s):  
Root Canal ◽  
Periodontal Ligament ◽  
Fibroblast Cells ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Mangosteen Peel ◽  
Human Periodontal Ligament Fibroblast ◽  
Irrigation Solution ◽  
Periodontal Ligament Fibroblast ◽  
Peel Extract

Background: Root canal irrigation is an important stage in root canal treatment as it is requires to eliminate necrotic and debris tissue as well as root canal wetting. Unfortunately, root canal irrigation can cause the material utilised to pass into the apical foramen leading to periapical complications. Consequently, the irrigation solution should have low toxicity. Sodium hypochlorite (NaOCl) is a commonly used irrigation solution since it has antibacterial properties. Moreover, NaOCl is also known to have the ability to dissolve necrotic tissue, vital pulp tissue and organic components of dentin and biofilms. Nevertheless, it can still cause damage when coming into contact with periapical tissues. On the other hand, Mangosteen peel extract (Garcinia mangostana L.), also has antibacterial activities. Hence, Mangosteen peel extract is assumed to be employable as an alternative irrigation solution. Purpose: This research aimed to reveal the toxicity levels of NaOCl and Mangosteen peel extract (Garcinia mangostin L.) used as irrigation solution in human periodontal ligament fibroblast cells (HPdLFc). Methods: HPdLFc were obtained from periapical tissues taken from one third of the first premolar teeth cultured. These cells were subsequently divided into several groups exposed to NaOCl and Mangosteen peel extract at certain concentrations. A toxicity test was then conducted using MTT assay. The results were analyzed with an Elisa reader. Cell deaths and LC50 were then calculated. Results: NaOCl became toxic at a concentration of 0.254 µl/ml or 0.025%, while Mangosteen peel extract became so at one of 2.099 ug/ml or 0.209%. Conclusion: NaOCl can be toxic at a concentration of 0.254 µl/ml or 0.025% and Mangosteen peel extract at one of 2.099 μg/ml or 0.209%.

scholarly journals Cytotoxicity assay of sodium hypochlorite and QMix on cultured human periodontal ligament fibroblast cells

2019 ◽  
Vol 11 (4) ◽  
pp. 204
Author(s):  
DianAgustin Wahjuningrum ◽  
MakkunraiEka Kramatawati Elizabeth ◽  
FikariniHadi Puteri ◽  
AndiAinul Mardiyah ◽  
Ari Subiyanto
Keyword(s):  
Sodium Hypochlorite ◽  
Periodontal Ligament ◽  
Cytotoxicity Assay ◽  
Fibroblast Cells ◽  
Human Periodontal Ligament Fibroblast ◽  
Periodontal Ligament Fibroblast

scholarly journals The effects of different 650 nm laser diode irradiation times on the viability and proliferation of human periodontal ligament fibroblast cells

2019 ◽  
Vol 52 (3) ◽  
pp. 142
Author(s):  
Kun Ismiyatin ◽  
Ari Subiyanto ◽  
Ika Tangdan ◽  
Rahmi Nawawi ◽  
Reinold C. Lina ◽  
...  
Keyword(s):  
Laser Diode ◽  
Periodontal Ligament ◽  
Clinical Manifestations ◽  
Fibroblast Cells ◽  
Pulp Tissue ◽  
Treatment Groups ◽  
Irradiation Group ◽  
Significant Difference ◽  
Human Periodontal Ligament Fibroblast ◽  
Periodontal Ligament Fibroblast

Background: Endo-perio lesions are clinical manifestations of inflammation in the periodontal and pulp tissue. Damage to the periodontal ligament can inhibit its ability to regenerate. Therefore, laser therapy use is expected to improve the prognosis with regard to healing lesions. Unfortunately, the duration of irradiation during laser diode therapy can influence the viability and proliferation of human periodontal ligament fibroblast (hPDLF) cells. Purpose: This study aims to determine the effects of different irradiation exposure times of the 650 nm laser diode of the pulsed mode type on the viability and proliferation of human periodontal ligament fibroblast cells. Methods: This study constituted a laboratory experiment on hPDLF cells using 650 nm laser diode irradiation. Six groups formed the research subjects in this study, namely; two control groups, two radiation groups respectively subjected to irradiation exposure of 15 seconds and 35 seconds duration followed by 24-hour incubation, and two radiation groups exposed to irradiation for 15 and 35 seconds respectively followed by 72-hour incubation period. The viability and proliferation of those cells were subsequently calculated by ELISA reader, while the data was analyzed by means of one-way ANOVA and Tukey tests. Results: The significance value of the viability scores between the 15-second irradiation group and the 35-second irradiation group was less than 0.05, indicating that there was a significant difference between these treatment groups. Similarly, the significance value of proliferation scores between the 15-second irradiation group and the 35-second irradiation group was less than 0.05, again indicating a significant difference between these treatment groups. Conclusion: Irradiation using a 650 nm laser diode 15 seconds and 35 seconds in duration can induce an increase in the viability and proliferation of hPDLF cells.

SITOTOKSISITAS EKSTRAK DAUN MANGROVE DARUJU (Acanthus ilicifolius) SEBAGAI BAHAN IRIGASI SALURAN AKAR

DENTA ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 44
Author(s):  
Twi Agnita Cevanti ◽  
Ratna Putri ◽  
Henu Sumekar
Keyword(s):  
Cell Viability ◽  
Root Canal ◽  
Fibroblast Cell ◽  
Chloroform Extract ◽  
Canal Irrigation ◽  
Whitney Test ◽  
Root Canal Irrigation ◽  
Mann Whitney Test ◽  
Acanthus Ilicifolius ◽  
Irrigation Solution

<p><strong><em>Background:</em></strong><em> The cleaning and shaping of the root canal are important stages in root canal treatment. The irrigation solution needed in those stages to clean the canal from residual necrotic tissue, demtine particles, and microorganism. Irrigation solution must fulfill some criteria among others, able to solute the debris or tissue residual, has low surface tension and not toxic. Mangrove Daruju (Acanthus ilicifolius) has potency to be a alternative of the usual irrigation solution because has antibacterial effect. <strong>Purpose: </strong>The aim of this study was to examine the cytotoxicity of Daruju mangrove (Acanthus ilicifolius) as root canal irrigation against the fibroblast cell (BHK-21) culture. <strong>Methods: </strong>The samples used was fibroblast cells (BHK-21) using culture method. These samples were treated with Acanthus ilicifolius chloroform extract with several concentration. Samples were divided into 1: cell control, 2:media control, 3: 40mg/ml, 4: 50mg/ml, 5: 60mg/ml,  6: 70mg/ml,: 80mg/ml, 7: control. MTT was added, 3 minutes after that incubated for 4 hours. DMSO solution was added and then shaken, the samples were analyzed using ElISA reader with a 620 wavelength. The cytotoxiciy wa expressed by cell viability. If its is &gt; 50%, it is declared as non toxic. Data analyzed using non parametric test (Kruskal-Wallis) followed by Mann Whitney test. <strong>Result: </strong>The Kruskal-Wallis test proved that there were significant differences in the cell viability among the treated groups. The average difference among the treated groups which were tested with Mann-Whitney test, showed a significant difference between group 1 and 5, also group 2 and group 5. <strong>Conclusions: </strong>Acanthus ilicifolius leaf chloroform extract has no cytotoxicity effect on concentration fibroblast cell (BHK-21) culture.</em></p><p><strong><em>Keywords: </em></strong><em>Acanthus ilicifolius, root canal irrigation, fibroblast cell</em></p><p><em><strong>Correspondence</strong>: Twi Agnita Cevanti, Laboratorium Konservasi Fakultas Kedokteran Gigi Universitas Hang Tuah, Jl, Arif Rahman Hakim 150, Surabaya, Indonesia. Ph 031-5945864, fax: 031-5912191, e-mail address: [email protected]</em></p>

scholarly journals The differences in root canal smear layer removal between 6,25% pineapple (Ananas comocus L. Merr.) peel extract and 17% Ethylene diamine tetra-acetic acid

2019 ◽  
Vol 52 (3) ◽  
pp. 122
Author(s):  
Nirawati Pribadi ◽  
Karlina Samadi ◽  
Meliavita N. K. Astuti ◽  
Hendy J. Kurniawan ◽  
Adelina K. Tandadjaja ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Root Canal ◽  
Smear Layer ◽  
Control Group ◽  
Canal Irrigation ◽  
Layer Removal ◽  
Root Canal Irrigation ◽  
Group I ◽  
Pineapple Peel ◽  
Peel Extract

Background: The smear layer is attached to dentine and occludes the orifice and, consequently, must be removed in order to improve the success of inroot canal treatment. The ideal irrigation material removes both the organic and inorganic smear layers. Ethylene diamine tetra-acetic acid (EDTA) is one of the most commonly used root canal irrigation materials, but removes only inorganic smear layer. To overcome this problem, Pineapple (Ananas comosus L. Merr.) peel extract, which contains saponins, bromelain, polyphenol and flavonoid, is used during root canal irrigation. Purpose: The study aimed to analyze the difference in smear layer removal between the use of 6.25% pineapple peel extract and 17% EDTA. Methods: 27 samples of mandibular premolar teeth with straight root canals were divided randomly into three groups (n = 9) and subsequently prepared using protaper. Irrigation was performed on the control group (aquadest), group I (17% EDTA) and group II (6.25% pineapple peel extract). The samples were dried, temporarily compressed and cut horizontally from the apical to the coronal. Samples were fixed with holder before the smear layer was observed through a scanning electron microscope (SEM). The resulting data was analyzed by means of an ANOVA test. Results: The highest score of root canal hygiene was recorded by group II, followed by group I and, finally, the control group. There were significant differences between the groups (p< 0.000). Conclusion: 6.25% pineapple peel extract produces a higher smear layer removal effect than 17%EDTA on the apical 1/3 of the root canal.

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