SITOTOKSISITAS EKSTRAK DAUN MANGROVE DARUJU (Acanthus ilicifolius) SEBAGAI BAHAN IRIGASI SALURAN AKAR

DENTA ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 44
Author(s):  
Twi Agnita Cevanti ◽  
Ratna Putri ◽  
Henu Sumekar
Keyword(s):  
Cell Viability ◽  
Root Canal ◽  
Fibroblast Cell ◽  
Chloroform Extract ◽  
Canal Irrigation ◽  
Whitney Test ◽  
Root Canal Irrigation ◽  
Mann Whitney Test ◽  
Acanthus Ilicifolius ◽  
Irrigation Solution

<p><strong><em>Background:</em></strong><em> The cleaning and shaping of the root canal are important stages in root canal treatment. The irrigation solution needed in those stages to clean the canal from residual necrotic tissue, demtine particles, and microorganism. Irrigation solution must fulfill some criteria among others, able to solute the debris or tissue residual, has low surface tension and not toxic. Mangrove Daruju (Acanthus ilicifolius) has potency to be a alternative of the usual irrigation solution because has antibacterial effect. <strong>Purpose: </strong>The aim of this study was to examine the cytotoxicity of Daruju mangrove (Acanthus ilicifolius) as root canal irrigation against the fibroblast cell (BHK-21) culture. <strong>Methods: </strong>The samples used was fibroblast cells (BHK-21) using culture method. These samples were treated with Acanthus ilicifolius chloroform extract with several concentration. Samples were divided into 1: cell control, 2:media control, 3: 40mg/ml, 4: 50mg/ml, 5: 60mg/ml,  6: 70mg/ml,: 80mg/ml, 7: control. MTT was added, 3 minutes after that incubated for 4 hours. DMSO solution was added and then shaken, the samples were analyzed using ElISA reader with a 620 wavelength. The cytotoxiciy wa expressed by cell viability. If its is &gt; 50%, it is declared as non toxic. Data analyzed using non parametric test (Kruskal-Wallis) followed by Mann Whitney test. <strong>Result: </strong>The Kruskal-Wallis test proved that there were significant differences in the cell viability among the treated groups. The average difference among the treated groups which were tested with Mann-Whitney test, showed a significant difference between group 1 and 5, also group 2 and group 5. <strong>Conclusions: </strong>Acanthus ilicifolius leaf chloroform extract has no cytotoxicity effect on concentration fibroblast cell (BHK-21) culture.</em></p><p><strong><em>Keywords: </em></strong><em>Acanthus ilicifolius, root canal irrigation, fibroblast cell</em></p><p><em><strong>Correspondence</strong>: Twi Agnita Cevanti, Laboratorium Konservasi Fakultas Kedokteran Gigi Universitas Hang Tuah, Jl, Arif Rahman Hakim 150, Surabaya, Indonesia. Ph 031-5945864, fax: 031-5912191, e-mail address: [email protected]</em></p>

scholarly journals Potential of mangrove (Acanthus Ilicifolius) leave extract as an alternative root canal irrigation in removing smear layer (in-vitro study)

2019 ◽  
Vol 10 (4) ◽  
pp. 2869-2874
Author(s):  
Dennis D ◽  
Winda Fatika Sari ◽  
Trimurni Abidin ◽  
Widi Prasetia
Keyword(s):  
Root Canal ◽  
Negative Control ◽  
Smear Layer ◽  
Control Group ◽  
Kappa Statistics ◽  
Control Group Design ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Acanthus Ilicifolius ◽  
Control Samples

Factor that should be considered in the use of irrigation is the loss of the smear layer on root canal walls. Therefore the prepared root canal can be irrigated using materials, such as EDTA 17% and 2,5% NaOCl and of extract Acanthus ilicifolius 7,5% and 10%. To determine the potential of extract Acanthus ilicifolius leaves as an alternative of root canal irrigation in removing smear layer at a concentratiom of 7,5% and 10%. The type of this study is a laboratory experimental with post-test only control group design. The total sample is 24 teeth samples that have been prepared and irrigated consisting of four groups. 6 samples for irrigation 7,5% extract Acanthus ilicifolius, 6 samples for irrigation 10% extract Acanthus ilicifolius, 6 positive control samples for combination 2,5% NaOCl and EDTA 17%, and 6 negative control samples. Shooting tool used to observe the cleanliness level in the walls of the root canal is a Scanning Electron Microscope (SEM). Data were analyzed using Kappa statistics, followed by Kruskal wallis analysis test and continued with Mann-Whitney analysis. Acanthus ilicifolius 7.5% and 10% extracts can remove the smear layer when compared with the negative control group. The combination of 2.5% NaOCl and 17% EDTA is better in smear layer removal compared to 7.5% and 10% Acanthus ilicifolius extracts. Acanthus ilicifolius was able to clean the smear layer on the root canal and can be used as an alternative root canal irrigation.

scholarly journals Cytoxicity test of NaOCl and Mangosteen (Garcinia Mangostin L.) peel extract used as an irrigation solution in human periodontal ligament fibroblast cells (HPdLFc)

2018 ◽  
Vol 51 (3) ◽  
pp. 133
Author(s):  
Tamara Yuanita ◽  
Dina Ristyawati ◽  
Karlina Samadi
Keyword(s):  
Root Canal ◽  
Periodontal Ligament ◽  
Fibroblast Cells ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Mangosteen Peel ◽  
Human Periodontal Ligament Fibroblast ◽  
Irrigation Solution ◽  
Periodontal Ligament Fibroblast ◽  
Peel Extract

Background: Root canal irrigation is an important stage in root canal treatment as it is requires to eliminate necrotic and debris tissue as well as root canal wetting. Unfortunately, root canal irrigation can cause the material utilised to pass into the apical foramen leading to periapical complications. Consequently, the irrigation solution should have low toxicity. Sodium hypochlorite (NaOCl) is a commonly used irrigation solution since it has antibacterial properties. Moreover, NaOCl is also known to have the ability to dissolve necrotic tissue, vital pulp tissue and organic components of dentin and biofilms. Nevertheless, it can still cause damage when coming into contact with periapical tissues. On the other hand, Mangosteen peel extract (Garcinia mangostana L.), also has antibacterial activities. Hence, Mangosteen peel extract is assumed to be employable as an alternative irrigation solution. Purpose: This research aimed to reveal the toxicity levels of NaOCl and Mangosteen peel extract (Garcinia mangostin L.) used as irrigation solution in human periodontal ligament fibroblast cells (HPdLFc). Methods: HPdLFc were obtained from periapical tissues taken from one third of the first premolar teeth cultured. These cells were subsequently divided into several groups exposed to NaOCl and Mangosteen peel extract at certain concentrations. A toxicity test was then conducted using MTT assay. The results were analyzed with an Elisa reader. Cell deaths and LC50 were then calculated. Results: NaOCl became toxic at a concentration of 0.254 µl/ml or 0.025%, while Mangosteen peel extract became so at one of 2.099 ug/ml or 0.209%. Conclusion: NaOCl can be toxic at a concentration of 0.254 µl/ml or 0.025% and Mangosteen peel extract at one of 2.099 μg/ml or 0.209%.

scholarly journals Antibacterial activity of Zingiber officinale roscoe extract as a potential root canal irrigation solution against Enterococcus faecalis

2018 ◽  
Vol 30 (2) ◽  
pp. 124
Author(s):  
Rodiyah Azhar ◽  
Elin Julianti ◽  
Setiawan Natasasmita ◽  
Hendra Dian Adhita Dharsono
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Cost Effective ◽  
Zingiber Officinale ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Zingiber Officinale Roscoe ◽  
Irrigation Solution

Introduction: This study was aimed to determine and formulate antibacterial activity of Zingiber Officinale Roscoe (Z. officinale) extract against Enterococcus faecalis (E. faecalis) as a potential irrigating solution which is easily available and cost-effective. Methods:  Extract of Z. officinale Roscoe was yielded through soxhlet technique. Samples divided into two groups, i.e., the extract of Z. officinale Roscoe and chlorhexidine 2% as a control. The antimicrobial activity was observed using the diffuse agar method. Minimum inhibitory concentration (MIC) was determined by serial microdilution method, and minimum bactericidal concentration (MBC) was determined by cultured samples in blood agar. Data were analysed using ANOVA and Kruskal-Wallis test. Results: Extract of Zingiber officinale Roscoe has antimicrobial activity against E. faecalis with the MBC of 15.625 mg/mL. (p<0,05). Conclusion: Extract of Zingiber officinale Roscoe have shown antibacterial activity against E. faecalis and can be further developed as a potential root canal irrigation solution.

scholarly journals Effectiveness of photon-initiated photoacoustic streaming in root canal models with different diameters or tapers

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Cheng Wen ◽  
Yuanyuan Kong ◽  
Jian Zhao ◽  
Yang Li ◽  
Ya Shen ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Root Canal ◽  
Distilled Water ◽  
Canal Irrigation ◽  
Root Canals ◽  
Atp Assay ◽  
Root Canal Irrigation ◽  
Average Fluorescence Intensity ◽  
Different Diameters ◽  
Average Fluorescence

Abstract Background This study aimed to compare the use of photon-initiated photoacoustic streaming (PIPS) and conventional needle irrigation (CNI) in conjunction with different concentrations of sodium hypochlorite (NaOCl) to remove Enterococcus faecalis (E. faecalis) suspended bacteria and biofilms from root canal systems with different diameters or tapers. Methods Artificial root canal samples (n = 480) were randomly divided into three groups (n = 160/group). The canals were prepared to fit file sizes #10/.02, #25/.02, or #25/.06. The size #10/.02 group was incubated for seven days. The size #25/.02 or #25/.06 group was incubated for 2 days. A stable biological model of E. faecalis infection was established. The root canals were washed with distilled water or with 1%, 2%, or 5.25% NaOCl combined with CNI or PIPS. Bacterial suspensions and biofilms were assessed using an ATP assay kit and fluorescence microscopy. Image-Pro Plus was used to analyse the average fluorescence intensity to determine the most suitable root canal irrigation solution. Results In the CNI and PIPS groups, the ATP value of the 5.25% NaOCl subgroup was the lowest, followed by that of the 2% and 1% NaOCl subgroups. The ATP value of the distilled water subgroup was the highest (P < 0.05). When the root canal taper was 0.02, the ATP value of the #10/.02 + PIPS group was significantly lower than that of the #25/.02 + CNI group (P < 0.05). The average fluorescence intensity of the #10/.02 + PIPS group was lower than that of the #25/.02 + CNI group (P < 0.05). When the apical diameter was #25, the ATP value of the 0.02 taper in the PIPS group was lower than that of the 0.06 taper in the CNI group (P < 0.05), and the average fluorescence intensity of the 0.02 taper + PIPS group was lower than that of the 0.06 taper + CNI group (P < 0.05). PIPS combined with 2% and 5.25% NaOCl effectively improved the long-term antibacterial effect after irrigation and re-culture for 6 h. Conclusions Compared with CNI, PIPS has greater ability to remove bacteria in root canals with a small preparation diameter and a small taper. PIPS with 2% and 5.25% NaOCl exhibited superior antibacterial and bacteriostatic effects.

Root Canal Irrigation

2015 ◽  
pp. 259-301 ◽  
Author(s):  
Luc van der Sluis ◽  
Christos Boutsioukis ◽  
Lei-Meng Jiang ◽  
Ricardo Macedo ◽  
Bram Verhaagen ◽  
...  
Keyword(s):  
Root Canal ◽  
Canal Irrigation ◽  
Root Canal Irrigation

Resistance to vertical root fracture of apicoected teeth using different devices during two root canal irrigation procedures

2018 ◽  
Vol 33 (8) ◽  
pp. 1685-1691 ◽  
Author(s):  
Ferhat Ayrancı ◽  
Leyla Benan Ayrancı ◽  
Alper Özdoğan ◽  
Serkan Özkan ◽  
Mutlunur Önder Peker ◽  
...  
Keyword(s):  
Root Canal ◽  
Root Fracture ◽  
Canal Irrigation ◽  
Vertical Root Fracture ◽  
Root Canal Irrigation
Sign in / Sign up

Export Citation Format

Share Document