scholarly journals Effect of Combination Yeast Bread and Chlorella sp. to Population Growth and Protein Content of Brachionus plicatilis

2020 ◽  
Vol 8 (1) ◽  
pp. 26
Author(s):  
Ike Yunita Indra Swari ◽  
Boedi Setya Rahardja ◽  
Prayogo Prayogo

One of larvae feed can be used is Brachionus plicatilis but necessary an enrichment for review adding nutritional values of the Brachionus plicatilis. Some phytoplankton frequently used type food by Brachionus plicatilis between lay Tetraselmis sp., Skeletonema costatum, Chlorella sp., Dunaliella salina and Spirulina sp. (Isnansetyo And Kurniastuty, 1995). In this time, many parlors seed microalgae replace with yeast bread, beside that yeast bread serves as a probiotic too (Winasuria, 1993). This study aims to determine the effect feeding review form a combination of yeast bread and Chlorella sp. against population growth and protein content of Brachionus plicatilis. The research method used is an experimental method with completely randomized design as design of Experiments. The treatment used is a combination of yeast and Chlorella sp. with different period, that was treatment A (Chlorella sp. 100%), treatment B (Chlorella sp. 25% and 75% baker's yeast), treatment C (Chlorella sp. 50% and 50 % of baker's yeast), treatment D (Chlorella sp. 75% and 25% baker's yeast), each treatment was repeated 5 times. The main parameter observed is population growth, growth rate and protein content. The supporting parameters observed parameter is water quality. Data analysis using variant analysis (ANOVA) and to review the best treatment, Duncan's Multiple Range Test performed. Results Provision combination of baker's yeast and Chlorella sp. with term certain showed significant differences (p<0,05) against population growth Brachionus plicatilis. The highest population growth are in treatment D  and the lowest are in treatment B. For a review feeding of Brachionus plicatilis preferably using Chlorella sp. with 1,125x106 density cells / ml and 0,0005 grams of baker's yeast.

2018 ◽  
Vol 15 (2) ◽  
Author(s):  
Iksan, Muhammad Junaidi Dan Alis Mukhlis

ABSTRAKThis study aims to determine the best dose of bakers yeast in culture Brachionus plicatilis. The research was conducted in July-September 2015 in the Mandiri Laboratory of BTN Puri Meninting West Lombok District. This experiment  uses a completely randomized design ( CRD ), which consists of 6 treatments and repeated 3 times , in order to obtain 18 units of trial that treatment A : without feed , treatment B : feed Nannochloropsis spp., treatment C : 7.5 mg/L, treatment D : 15.0 mg/L , treatment E : 22.5 mg/L , and treatment F : 30.0 mg/L. The results showed that the difference in the level of baker's yeast significantly affect the population growth of Brachionus plicatilis, which obtained the highest population density in the yeast dose of 30.0 mg/L of 90 individuals/mL Key word : bakers yeast, population growth, Brachionus plicatilisABSTRACT Penelitian ini bertujuan untuk mengetahui dosis ragi roti yang terbaik  dalam kultur Brachionus plicatilis. Penelitian dilaksanakan pada bulan Juli - September 2015  di Laboratorium Mandiri BTN Puri Meninting Kabupaten Lombok Barat. Penelitian ini menggunakan  Rancangan Acak Lengkap (RAL) yang terdiri dari 6 perlakuan dan diulang sebanyak 3 kali, sehingga diperoleh 18 unit percobaan yaitu  perlakuan  A : tanpa pakan, perlakuan  B : pakan Nannochloropsis spp.,  perlakuan C : 7,5 mg/L, perlakuan D : 15,0 mg/L, perlakuan  E :  22,5 mg/L,  dan perlakuan  F : 30,0 mg/L. Hasil penelitian menunjukan bahwa perbedaan dosis ragi roti berpengaruh nyata terhadap pertumbuhan populasi Brachionus plicatilis, dimana kepadatan populasi tertinggi diperoleh pada dosis ragi 30,0 mg/L yaitu 90 individu/mL Kata kunci : ragi roti, pertumbuhan populasi,  Brachionus plicatilis,


2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Iksan, Muhammad Junaidi Dan Alis Mukhlis

ABSTRAKPenelitian ini bertujuan untuk mengetahui dosis ragi roti yang terbaik  dalam kultur Brachionus plicatilis. Penelitian dilaksanakan pada bulan Juli - September 2015  di Laboratorium Mandiri BTN Puri Meninting Kabupaten Lombok Barat. Penelitian ini menggunakan  Rancangan Acak Lengkap (RAL) yang terdiri dari 6 perlakuan dan diulang sebanyak 3 kali, sehingga diperoleh 18 unit percobaan yaitu  perlakuan  A : tanpa pakan, perlakuan  B : pakan Nannochloropsis spp.,  perlakuan C : 7,5 mg/L, perlakuan D : 15,0 mg/L, perlakuan  E :  22,5 mg/L,  dan perlakuan  F : 30,0 mg/L. Hasil penelitian menunjukan bahwa perbedaan dosis ragi roti berpengaruh nyata terhadap pertumbuhan populasi Brachionus plicatilis, dimana kepadatan populasi tertinggi diperoleh pada dosis ragi 30,0 mg/L yaitu 90 individu/mL Kata kunci : ragi roti, pertumbuhan populasi,  Brachionus plicatilis, ABSTRACT This study aims to determine the best dose of bakers yeast in culture Brachionus plicatilis. The research was conducted in July-September 2015 in the Mandiri Laboratory of BTN Puri Meninting West Lombok District. This experiment  uses a completely randomized design ( CRD ), which consists of 6 treatments and repeated 3 times , in order to obtain 18 units of trial that treatment A : without feed , treatment B : feed Nannochloropsis spp., treatment C : 7.5 mg/L, treatment D : 15.0 mg/L , treatment E : 22.5 mg/L , and treatment F : 30.0 mg/L. The results showed that the difference in the level of baker's yeast significantly affect the population growth of Brachionus plicatilis, which obtained the highest population density in the yeast dose of 30.0 mg/L of 90 individuals/mL Key word : bakers yeast, population growth, Brachionus plicatilis


2015 ◽  
Vol 15 (2) ◽  
Author(s):  
Iksan, Muhammad Junaidi Dan Alis Mukhlis

ABSTRACT This study aims to determine the best dose of bakers yeast in culture Brachionus plicatilis. The research was conducted in July-September 2015 in the Mandiri Laboratory of BTN Puri Meninting West Lombok District. This experiment  uses a completely randomized design ( CRD ), which consists of 6 treatments and repeated 3 times , in order to obtain 18 units of trial that treatment A : without feed , treatment B : feed Nannochloropsis spp., treatment C : 7.5 mg/L, treatment D : 15.0 mg/L , treatment E : 22.5 mg/L , and treatment F : 30.0 mg/L. The results showed that the difference in the level of baker's yeast significantly affect the population growth of Brachionus plicatilis, which obtained the highest population density in the yeast dose of 30.0 mg/L of 90 individuals/mL Key word : bakers yeast, population growth, Brachionus plicatilis ABSTRAKPenelitian ini bertujuan untuk mengetahui dosis ragi roti yang terbaik  dalam kultur Brachionus plicatilis. Penelitian dilaksanakan pada bulan Juli - September 2015  di Laboratorium Mandiri BTN Puri Meninting Kabupaten Lombok Barat. Penelitian ini menggunakan  Rancangan Acak Lengkap (RAL) yang terdiri dari 6 perlakuan dan diulang sebanyak 3 kali, sehingga diperoleh 18 unit percobaan yaitu  perlakuan  A : tanpa pakan, perlakuan  B : pakan Nannochloropsis spp.,  perlakuan C : 7,5 mg/L, perlakuan D : 15,0 mg/L, perlakuan  E :  22,5 mg/L,  dan perlakuan  F : 30,0 mg/L. Hasil penelitian menunjukan bahwa perbedaan dosis ragi roti berpengaruh nyata terhadap pertumbuhan populasi Brachionus plicatilis, dimana kepadatan populasi tertinggi diperoleh pada dosis ragi 30,0 mg/L yaitu 90 individu/mL Kata kunci : ragi roti, pertumbuhan populasi,  Brachionus plicatilis, 


2015 ◽  
Vol 15 (2) ◽  
Author(s):  
Iksan, Muhammad Junaidi Dan Alis Mukhlis

ABSTRAKThis study aims to determine the best dose of bakers yeast in culture Brachionusplicatilis. The research was conducted in July-September 2015 in the Mandiri Laboratory ofBTN Puri Meninting West Lombok District. This experiment uses a completely randomizeddesign ( CRD ), which consists of 6 treatments and repeated 3 times , in order to obtain 18units of trial that treatment A : without feed , treatment B : feed Nannochloropsis spp.,treatment C : 7.5 mg/L, treatment D : 15.0 mg/L , treatment E : 22.5 mg/L , and treatment F :30.0 mg/L. The results showed that the difference in the level of baker's yeast significantlyaffect the population growth of Brachionus plicatilis, which obtained the highest populationdensity in the yeast dose of 30.0 mg/L of 90 individuals/mLKey word : bakers yeast, population growth, Brachionus plicatilisABSTRACTPenelitian ini bertujuan untuk mengetahui dosis ragi roti yang terbaik dalam kulturBrachionus plicatilis. Penelitian dilaksanakan pada bulan Juli - September 2015 diLaboratorium Mandiri BTN Puri Meninting Kabupaten Lombok Barat. Penelitian inimenggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 6 perlakuan dan diulangsebanyak 3 kali, sehingga diperoleh 18 unit percobaan yaitu perlakuan A : tanpa pakan,perlakuan B : pakan Nannochloropsis spp., perlakuan C : 7,5 mg/L, perlakuan D : 15,0mg/L, perlakuan E : 22,5 mg/L, dan perlakuan F : 30,0 mg/L. Hasil penelitian menunjukanbahwa perbedaan dosis ragi roti berpengaruh nyata terhadap pertumbuhan populasiBrachionus plicatilis, dimana kepadatan populasi tertinggi diperoleh pada dosis ragi 30,0mg/L yaitu 90 individu/mLKata kunci : ragi roti, pertumbuhan populasi, Brachionus plicatilis,


Author(s):  
Ida N Jamal ◽  
Reiny A Tumbol ◽  
Remy E.P Mangindaan

Motile Aeromonas Septicaemia disease (MAS) attacking tilapia has increased in recent years as a consequence of intensive aquaculture activities, which led to losses in aquaculture industry. The agent causing MAS disease is Aeromonas hydrophila. The disease can be controlled with the β-glucan. As immunostimulants, β-glucans can also increase resistance in farmed tilapia. Studies on the use of β-glucan extracted from baker's yeast Saccharomyces cerevisiae was intended to evaluate the non-specific immune system of tilapia that were challenged with Aeromonas hydrophila. The method used was an experimental method with a completely randomized design consisting of four treatments with three replicats. The dose of β-glucan used as treatments were 0 mg.kg-1 fish (Control), 5 mg.kg-1 fish (B), 10 mg.kg-1 fish (C) and 20 mg.kg-1 fish (D), each treatment as injected three times at intervals of 3 days, the injection volume of 0.5 ml/fish for nine days and resistance surveillance for seven days. The results showed that the difference in the amount of β-glucan and the frequency of the injected real influence on total leukocytes, phagocytic activity and resistance. Total leukocytes, phagocytic activity and resistance to treatment was best achieved by the administration of C a dose of  10 mg.kg-1 of the fish© Penyakit Motil Aeromonas Septicaemia (MAS) yang menyerang ikan nila mengalami peningkatan selama beberapa tahun terakhir sebagai konsekuensi dari kegiatan akuakultur intensif, yang menyebabkan kerugian dalam industri budidaya. Agen utama penyebab penyakit MAS adalah Aeromonas hydrophila. Untuk mengendalikan penyakit tersebut dapat dilakukan dengan pemberian β-glukan. Sebagai imunostimulan, β-glukan juga dapat  meningkatkan resistensi pada ikan nila yang dibudidayakan. Pengkajian mengenai pemanfaatan β-glukan yang diekstrak dari ragi roti Saccharomyces cerevisiae dimaksudkan untuk menguji sistem imun non spesifik ikan nila yang diuji tantang dengan bakteri Aeromonas hydrophila. Metode yang digunakan yaitu metode eksperimen dengan rancangan acak lengkap yang terdiri dari empat perlakuan dan tiga ulangan. Dosis β-glukan  yang digunakan sebagai perlakuan sebesar 0 mg.kg-1 ikan (Kontrol), 5 mg.kg-1 ikan (B), 10 mg.kg-1 ikan (C) dan 20 mg.kg-1 ikan (D), masing-masing perlakuan diinjeksi sebanyak 3 kali dengan interval waktu 3 hari selama 9 hari, volume injeksi 0,5 mL/ekor ikan dan pengamatan resistensi selama tujuh hari. Hasil penelitian menunjukkan perbedaan jumlah β-glukan dan frekuensi pemberian yang diinjeksikan memberikan pengaruh nyata terhadap total leukosit, aktivitas fagositosis dan resistensi. Total leukosit, aktivitas fagositosis dan resistensi terbaik dicapai pada perlakuan C dengan dosis 10 mg.kg-1 ikan©


2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Phyu Phyu San ◽  
Midori Tuda ◽  
Kengo Nakahira ◽  
Masami Takagi

Abstract Background The successful rearing of predators or parasitoids is one of the most important elements in biological control programs. The dried fruit mite, Carpoglyphus lactis (L.), can be used as an alternative prey for the phytoseiid predatory mite, Amblyseius swirs0kii (Athias-Henriot). Main body This study aimed to evaluate the suitability of 3 different diets for C. lactis when rearing A. swirskii: brown sugar, baker’s yeast, and the mixture of brown sugar and baker’s yeast along with 3 initial predator:prey ratios (1:7, 1:12, and 1:20). After 30 days, both the baker’s yeast and the mixture of brown sugar + baker’s yeast diets resulted in the highest predator densities (40.5 times increase at an initial predator:prey ratio of 1:20, with the baker’s yeast diet), whereas the mixture diet produced the highest density of prey. The brown sugar resulted in the lowest number of predator and prey mites. This may be due to lower feeding and predation rates of the prey and the predator mites on the brown sugar medium since its relative moisture content and adhesiveness inhibits mite movement. The final predator:prey ratio was also highest on the baker’s yeast diet. Conclusions This study demonstrated that the population growth of A. swirskii was the highest when C. lactis was reared on baker’s yeast.


2017 ◽  
Vol 4 (2) ◽  
pp. 65
Author(s):  
Indra Sahputra ◽  
Munawwar Khalil ◽  
Zulfikar Zulfikar

Penelitian ini dilaksanakan padatanggal 1 Juni – 1 Juli 2014 di Tambak Daerah Cot Kafiraton Kecamatan Seunuddon, Kabupaten Aceh Utara. Metode yang digunakan dalam penelitian ini adalah metode ekperimental dengan menggunakan rancangan acak lengkap (RAL) Non Faktorial dengan lima perlakukan dan tiga kali ulangan yaitu perlakuan A: pemberian pakan jenis udang dogol perlakuan B: pemberian pakan jenis benih ikan nila; perlakuan C: pemberian pakan jenis keong mas ; perlakuan D: pemberian pakan pellet komersial. Parameter uji dalam penelitian ini adalah tingkat kelangsungan hidup, pertumbuhan, kecepatan konsumsi pakan dan kualitas air. Data hasil penelitian dianalisis secara deskriptif serta diuji dengan beda nyata terkecil (BNT). Hasil penelitian menunjukkan bahwa pemberian pakan alami yang berbeda memberi pengaruh yang sangat berbeda nyata terhadap pertumbuhan dan konsumsi pakan pada ikan kakap putih dimana Fhitung >Ftable yaitu pada perlakuan A. Akan tetapi tidak memberi pengaruh yang sangat berbeda nyata terhadap kelangsungan hidup ikan kakap putih. Nilai kualitas air selama penelitian yaitu baik dimana berada pada kisaran yang layak untuk kehidupan ikan kakap putih dengan pH 7,9-8,5, suhu berkisar 25-290C dan salinitas 23-26 ppt.The research was conducted on June 1 to July 1 2014 in Pond at Cot Kafiraton Seunuddon district, North Aceh. The experiment treatments was used on this study using a completely randomized design (CRD) non factorial with five treatments and three replicated which were A: feed types of dogol shrimp, B: feed  type of tilapia seed, treatment C: feed type of snails, treatment D: feed type commercial pellets. Parameters of this study was the survival rate, growth, feed consumption rate and water quality. Data were analyzed descriptively and tested by the least significant difference (LSD). The results was showed that different types of feed had very effect significantly different  on the growth and feed intake of sea bass (Fcal>Ftab). However, different fedd types did not give significantly different influence on the survival rate of sea bass. Water quality parameters were in suitable condition for sea bass habitats. The value of pH was 7,9-8,5, temperature 25-29 0C and salinity  23-26 ppt.


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