Technique of Increasing Calcium Carbonate (CaCO3) in White Shrimp (Litopenaeus vannamei) Feed in Kasetsart University, Bangkok

White shrimp (Litopenaeus vannamei) culture has grown rapidly in Indonesia since early 2000s. White shrimp have stronger resistance to WSSDV attacks, and are euryhaline. In it’s natural habitat, this shrimp was found in waters with a range of 0.5-40 ppt salinity. White shrimp grow optimally at 15-25 ppt salinity, even still suitable at 5 ppt salinity. This capability provides the opportunities to culture these commodities in inland water. White shrimp need inorganic elements or minerals for their living processes. Calcium is one of the main needs of shrimp that is closely related to the development and maintenance of skeletal systems and plays a role in some physiological processes of the body.The requirement for calcium in shrimp is increasing when it is cultivated in fresh water.This Internship aims to find out the technique of adding calcium carbonate (CaCO3) in feed, used for the white shrimp culture in fresh water. In this field practice, the addition of calcium carbonate in a level of 2% refers to the requirement of white shrimp for optimal growth. This was based on the calcium requirements of shrimp in the amount of 1-2%. The method was repelleting, by grinding feed and calcium using mortar and pestle, then filtered to get the smoothest part. Then mix calcium with 10 mL of water and mix it with pellets evenly. Then the mixture is air dried and mashed again and weighed according to the required dose of feed. The addition of calcium in the feed affects the growth of shrimp, because calcium is one of the most important anorganic mineral for the growth and development of exoskeleton, regulates blood clotting, heart rate, kidneys, nerves, the activity of several enzymes, and cell function

The Freezing Process of Red Snapper (Lutjanus malabaricus) with the Air Blast Freezing (ABF) Method at PT. Inti Luhur Fuja Abadi, Pasuruan, East Java

Fish is a protein source that is very potential and needed by humans. One of the fish that has a high enough nutrient content is Red Snapper (Lutjanus malabaricus). Snapper fish contains 92 kilocalories of energy, 20 grams of protein, 0 grams of carbohydrates, 0.7 grams of fat, 20 milligrams of calcium, 200 milligrams of phosphorus, and 1 milligram of iron. The high water content is one potential that must be considered because it is a good medium for microbial growth. Therefore efforts are needed to maintain the nutritional content, freshness, taste, and extend the shelf life through preservation, one of which is by freezing. The Air Blast Freezing method is one of the freezing methods that uses a combination of low temperatures which are temperatures of -35℃ to -40℃ by blowing cold air quickly towards the product. This method has the advantage of one of which is relatively easy operation. The purpose of this field work practice is to know the process of freezing Red Snapper (Lutjanus malabaricus) with the Air Blast Freezing (ABF) method and the obstacles in the production of Red Snapper (Lutjanus malabaricus) using the Air Blast Freezing (ABF) method. The stages of the production process of Red Snapper (Lutjanus malabaricus) are the reception of raw materials, washing I, sorting, washing II, weighing, washing III, preparation, freezing with the Air Blast Freezing (ABF) method, glazing, packaging, storaging, stuffing. The freezing process with the Air Blast Freezing (ABF) method uses a temperature of -35℃ to -40℃ for 6-8 hours so that the plant can produce as much as 3 tons in one day. Obstacles to the freezing process at PT ILUFA are raw materials that contain parasites. Factors that influence the freezing process include raw materials during the reception and monitoring process during the freezing process in the ABF engine.

Grouper Imotilization Technique Epinephelus sp. Using Datura Metel L. Seed Extract

This study is about fish anesthesia using natural anesthetic ingredients. Natural anesthetic ingredients aim to reduce metabolism in Epinephelus sp. grouper fish. The immobilization method can be used to minimize high metabolism in Epinephelus sp. grouper fish. The purpose of this study was to obtain the influence of Datura metel L. seed extract on the onset time, recovery time, and live graduation of grouper fish Epinephelus sp. The results of the study, using extracts of natural anesthetic ingredients Datura metel L.seed with concentrations of 25%, 30%, and 35% can be concluded that it has a different influence (P<0.05) on the onset and recovered grouper fish. This study gave a life graduation rate of 100% in each concentration. The best concentration in this study was 35% concentration, with the fastest onset time of 3 minutes with a recovery time of 10.67 minutes. This study used a non-Factorial Complete Randomized Design

Nursery Management of Cantang Grouper (Epinephelus fuscoguttatus-lanceolatus) at Convrete Pond in Balai Perikanan Budidaya Air Payau (BPBAP), Situbondo – East Java.

Cantang groupers is an Indonesian fishery commodity is superior and has a high economic value. Cantang groupers (Epinephelus fuscoguttatus-lanceolatus) is an engineered hybrid seeds of cross-breeding between male parent fish giant grouper (Epinephelus fuscoguttatus) with the female parent tiger grouper (Epinephelus lanceolatus). Balai Perikanan Budidaya Air Payau (BPBAP) is one of the balai that have nursed the grouper abstraction with quality seed yields. This Field Work Training with the aim to help financial management of the grouper abstraction in concrete tanks, the factors that need to be considered, challenges that arise in the process of separating grouper and business analysis, used descriptive method of retrieval primary and secondary data. Nursery of cantang groupers seed aims to similize so that it can reduce the level of cannibalism and enchance the fish’s endurance before cultivated on a pond or net cages. Things that need to be prepared in nursery grouping include preparation of containers, seed selection and stocking, grading, growth data collection, feed management, water quality management, pest and disease control, harvesting and marketing. From the results of Field Work Training, the optimal growth rate is 0.55 gr / day and 0.132 cm/day with an increase of 13.2% in 30 days. Optimal growth can occur due to good food management, water quality, and grading.

Packaging of Presto Milkfish in CV. Fania Food Yogyakarta

Milkfish is a commodity that is commonly consumed by the Indonesian people, in addition to its economical price because there are already many choices of milkfish products that have been innovated so that the community's interest in milkfish is increasing. The nutritional content of milkfish is quite high. Field work practices (PKL) are held in Yogyakarta starting from 17 December 2018 to 31 January 2019. At Cv. Fania Food Yogyakarta, on the road to Semanggu KG-1 No.16, Gedong Kuning, Kota Gede, Yogyakarta City, Yogyakarta Special Region. The method used in this field work practice is a descriptive method, namely fact finding with the right interpretation. Descriptive method is a method that addresses the human group of an object, a set of conditions, a system of thought, or a class of events in the present. The scope of packaging criteria includes the process of making presto fish and packaging milkfish presto. Aspects that include packaging criteria ranging from packaging materials, packaging labeling, list of materials used, weight or net contents, name and address of the party producing or entering food into the territory of Indonesia and expiration date, month and year and certification from the relevant agency.

Analysis of Cadmium (Cd) Heavy Metal Using the Atomic Abrsoption Spectrophotometry (AAS) Method in Canned Crab Products at Balai Pengujian Mutu Hasil Perikanan (BPMHP) Semarang, Central Java

Crab is fishery potential fishery product but it is a perishable food. Application and handling of crab can be done by processing it into canned products. Crab are very likely to be polluted by heavy metals in these waters because of heavy metal contamination is already very high in the ocean. The purpose of this activity is to find out directly assay methods of heavy cadmium (Cd) in canned crab product and know the standard limit of heavy metal cadmium (Cd) The analytical method of cadmium heavy metal content (Cd) on several canned crab products using the method analysis of SNI 2354.5: 2011. Based on the results of the crab product analytical (Portunus pelagicus) the results showed <0.5 mg / kg, so that based on the threshold of cadmium (Cd) on the tin crab pasteuriation product is regulated in SNI 6929: 2016, the product is safe and suitable for consumption.

Identification of Aeromonas Salmonicida and Edwardsiella Ictalury in Live Fish that Will Be Trafficked from Yogyakarta Special Region

The freshwater aquaculture fishery sector in Indonesia has the potential to be developed through extensification and intensification. The problem in aquaculture is diseases that infects cultivated organisms, one of which is caused by bacterial infection. Bacterial infection can cause huge losses to cultivation activities, including death which results in economic losses. The purpose of this study is to identify the A. salmonicida and E. ictalury in live fish that will be exported from DI Yogyakarta. The method of examining bacteria used is conventional which consists of isolation, purification, presumptive test, biochemical test, and identification. The results showed that all live fish samples (koi, gouramy, goldfish, catfish, and eel) were not infected with A. salmonicida and E. ictalury. This shows that all live fish are in a healthy condition and suitable to be exported of DI Yogyakarta.

Quality Control of Raw Materials on Red Snapper (Lutjanus sp.) Freezing Process in PT. Tridaya Jaya Manunggal Pasuruan East Java

Red snappers are fishery commodities which have high market values. Raw materials of red snappers are important elements which determine the quality of the final products. Therefore, an enhancement of raw materials quality should be conducted through quality supervision and examination when the raw materials are retrieved, stored, and about to enter production process. The aim of this fieldwork practice is to find out the raw materials quality control on red snapper freezing process in PT. Tridaya Jaya Manunggal Pasuruan, East Java. The data taken consists of primary and secondary data which are processed descriptively. Data collection techniques through observation, interviews, active participation and literature review. PT. Tridaya Jaya Manunggal has implemented quality control on production process, starting from raw materials retrieval until these raw materials become final products. The quality control of raw red snappers were done in accordance with the quality control procedures which are plan, do, check, and action. The results of implementing quality control which were executed with organoleptic examination, temperature, Total Volatile Base (TVB), microbiology, and heavy metals on raw red snappers. Raw materials which didn’t meet the required standard were rejected, and then they were processed to be second grade products.

Frozen Cuttlefish (Sepia officinalis) Production Process with Contact Plate Freezing Method at PT. Karya Mina Putra, Rembang, Central Java

Cuttlefish is widely consumed by the public because it has a soft meat texture and high nutritional content. This makes the cuttlefish one of the mollusca that is a source of protein in addition to squid and octopus. The disadvantage of cuttlefish is that it is prone to deterioration in quality. One effort to prevent quality deterioration in cuttlefish is by freezing. The purpose of the implementation of this Field Work Practice is to decide the production process of frozen cuttlefish (Sepia officinalis) using the Contact Plate Freezing (CPF) method and to know the obstacles that occur in the production process. Field Work Practice was held in PT. Karya Mina Putra, Rembang, Central Java. The stages of the frozen cuttlefish production process at PT. Mina Putra's work includes the reception of raw materials, weighing I and sorting, labeling I, weighing II, washing, pan preparation, freezing, glazing, packaging and labeling II, storing in cold storage, and loading. The freezing process is carried out by the Contact Plate Freezing (CPF) method for 6 hours until the temperature reaches -40 ° C. The obstacle in the production process of frozen cuttlefish is there was a Contact Plate Freezing (CPF) machine damage and there are some employees who do not apply the SOP applied to prevent cross-contamination of the product.

Application of Fucoxanthin Pigment Extract from Sargassum sp. on the Physical Quality of Blusher Preparation

Sargassum sp. is a brown algae (Phaeophyta) which contains fucoxanthin, i.e. a carotenoid pigment that can be developed as natural dyes. On the cheek coloring products (blusher), color is an important parameter that determines the consumer appeal. This study was carried out by extraction, purification, qualitative and quantitative analysis, making blusher preparation with addition of fucoxanthin pigment extract form Sargassum sp. of 4%, 5%, 6% and 7%, physical quality and antioxidant activity test in the best treatment. The results of this study indicated the application of fucoxanthin pigment extract from Sargassum sp. giving effect to the physical quality of blusher. This blusher preparation had good color homogeneity and almost the same particle size as the commercial blusher. It also had good color stability during 15 days of storage with a change in pH which increases between 8,32-9,34. The irritation reaction that occurred on 10 panelists were undetected and it had the highest hedonic value on blusher with the addition of 4% fucoxanthin. The best treatment in this study was blusher with 4% addition of fucoxanthin pigment extract. The 4% treatment had good homogeneity and color stability. The skin irritation was undetected, it had the most neutral pH value or matches with physiological pH value of the skin and had a moderate antioxidant activity with an IC50 value of 123,66 μg/mL and blusher had a shape like small chunks with a mean size of 36,79 μm in SEM analysis.