Salmonella enterica serotypes isolated from squabs reveal multidrug resistance and a distinct pathogenicity gene repertoire

2014 ◽  
Vol 33 (3) ◽  
pp. 997-1006 ◽  
Author(s):  
K.M. OSMAN ◽  
S.H. MAROUF ◽  
O.A. MEHANA ◽  
N. ALATFEEHY
Keyword(s):  
Multidrug Resistance ◽  
Salmonella Enterica ◽  
Gene Repertoire ◽  
Pathogenicity Gene

scholarly journals Population structure of Salmonella enterica serotype Mbandaka reveals similar virulence potential irrespective of source and phylogenomic stratification

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 1142
Author(s):  
Linto Antony ◽  
Gavin Fenske ◽  
Radhey S Kaushik ◽  
Tiruvoor G Nagaraja ◽  
Milton Thomas ◽  
...  
Keyword(s):  
Population Structure ◽  
Multidrug Resistance ◽  
Salmonella Enterica ◽  
Virulence Gene ◽  
Economic Stress ◽  
Phylogenomic Analysis ◽  
Gene Repertoire ◽  
Phenotypic Analysis ◽  
Virulence Potential ◽  
Foodborne Outbreaks

Background: Salmonella enterica serotype Mbandaka (Salmonella ser. Mbandaka) is a multi-host adapted Non-typhoidal Salmonella (NTS) that can cause foodborne illnesses in human. Outbreaks of Salmonella ser. Mbandaka contributed to the economic stress caused by NTS due to hospitalizations. Whole genome sequencing (WGS)-based phylogenomic analysis facilitates better understanding of the genomic features that may expedite the foodborne spread of Salmonella ser. Mbandaka. Methods: In the present study, we define the population structure, antimicrobial resistance (AMR), and virulence profile of Salmonella ser. Mbandaka using WGS data of more than 400 isolates collected from different parts of the world. We validated the genotypic prediction of AMR and virulence phenotypically using an available set of representative isolates. Results: Phylogenetic analysis of Salmonella ser. Mbandaka using Bayesian approaches revealed clustering of the population into two major groups; however, clustering of these groups and their subgroups showed no pattern based on the host or geographical origin. Instead, we found a uniform virulence gene repertoire in all isolates. Phenotypic analysis on a representative set of isolates showed a similar trend in cell invasion behavior and adaptation to a low pH environment. Both genotypic and phenotypic analysis revealed the carriage of multidrug resistance (MDR) genes in Salmonella ser. Mbandaka. Conclusions: Overall, our results show that the presence of multidrug resistance along with adaptation to broad range of hosts and uniformity in the virulence potential, isolates of Salmonella ser. Mbandaka from any source could have the potential to cause foodborne outbreaks as well as AMR dissemination.

scholarly journals Highly Resistant Salmonella enterica Serovar Typhi with a NovelgyrAMutation Raises Questions about the Long-Term Efficacy of Older Fluoroquinolones for Treating Typhoid Fever

2012 ◽  
Vol 56 (5) ◽  
pp. 2761-2762 ◽  
Author(s):  
Kanika Deshpande Koirala ◽  
Duy Pham Thanh ◽  
Sudeep Dhoj Thapa ◽  
Amit Arjyal ◽  
Abhilasha Karkey ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Typhoid Fever ◽  
Treatment Failure ◽  
Salmonella Enterica ◽  
Systemic Infection ◽  
Resistant Isolate ◽  
Content Type ◽  
Salmonella Enterica Serovar Typhi ◽  
Term Efficacy

ABSTRACTAs a consequence of multidrug resistance, clinicians are highly dependent on fluoroquinolones for treating the serious systemic infection typhoid fever. While reduced susceptibility to fluoroquinolones, which lessens clinical efficacy, is becoming ubiquitous, comprehensive resistance is exceptional. Here we report ofloxacin treatment failure in typhoidal patient infected with a novel, highly fluoroquinolone-resistant isolate ofSalmonella entericaserovar Typhi. The isolation of this organism has serious implications for the long-term efficacy of ciprofloxacin and ofloxacin for typhoid treatment.

scholarly journals Salmonella enterica Serovar Typhi Strains Isolated in Korea Containing a Multidrug Resistance Class 1 Integron

2003 ◽  
Vol 47 (6) ◽  
pp. 2006-2008 ◽  
Author(s):  
Hyunjoo Pai ◽  
Jeong-hum Byeon ◽  
Sunmi Yu ◽  
Bok Kwon Lee ◽  
Shukho Kim
Keyword(s):  
Multidrug Resistance ◽  
Resistance Genes ◽  
Salmonella Enterica ◽  
Multidrug Resistant ◽  
Conjugative Plasmid ◽  
The Novel ◽  
Class 1 Integron ◽  
Salmonella Enterica Serovar Typhi ◽  
Class 1 ◽  
Clonal Spread

ABSTRACT Six strains of Salmonella enterica serovar Typhi which were resistant to ampicillin, chloramphenicol, trimethoprim-sulfamethoxazole, streptomycin, tetracycline, and gentamicin were isolated in Korea. This multidrug resistance was transferred by a conjugative plasmid of about 50 kb. The plasmid harbored a class 1 integron, which included six resistance genes, aacA4b, catB8, aadA1, dfrA1, aac(6′)-IIa, and the novel blaP2, in that order. All of the isolates showed the same-size plasmids and the same ribotyping patterns, which suggests a clonal spread of these multidrug-resistant isolates.

PREVALENCE OF CLASS 1 INTEGRONS IN CLINICAL ISOLATES OF NON-TYPHOIDAL $SALMONELLA~ENTERICA$ CIRCULATED IN ARMENIA

2021 ◽  
Vol 55 (1 (254)) ◽  
pp. 58-66
Author(s):  
Magdalina K. Zakharyan
Keyword(s):  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
Clinical Significance ◽  
Antimicrobial Therapy ◽  
Salmonella Enterica ◽  
Clinical Isolates ◽  
Class 1 Integron ◽  
Class 1 Integrons ◽  
High Prevalence ◽  
Class 1

A total of 182 non-typhoid Salmonella enterica (NTS) isolates recovered from patients between 1996 and 2014 were included in the current study focused on class 1 integron detection and its association with multidrug resistance (MDR) phenotype. A high prevalence of isolates displaying MDR and penta-resistance (resistance to ampicillin, chloramphenicol, streptomycin, sulfonamide, tetracycline) phenotypes of clinical significance was revealed. Serotype-specific prevalence of antimicrobial resistance as well as class 1 integrons and inserted variable segments was detected in isolates. The results indicated the limitations of current antimicrobial therapy to control infections caused by MDR isolates of NTS, especially belonging to serotype Typhimurium.

scholarly journals Antimicrobial Resistance and Virulence Determinants in European Salmonella Genomic Island 1-Positive Salmonella enterica Isolates from Different Origins

2011 ◽  
Vol 77 (16) ◽  
pp. 5655-5664 ◽  
Author(s):  
Janine Beutlich ◽  
Silke Jahn ◽  
Burkhard Malorny ◽  
Elisabeth Hauser ◽  
Stephan Hühn ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Variable Number Tandem Repeat ◽  
Tetracycline Resistance ◽  
Variable Number ◽  
Virulence Determinants ◽  
Content Type ◽  
Strain Collection

ABSTRACTSalmonellagenomic island 1 (SGI1) contains a multidrug resistance region conferring the ampicillin-chloramphenicol-streptomycin-sulfamethoxazole-tetracycline resistance phenotype encoded byblaPSE-1,floR,aadA2,sul1, andtet(G). Its increasing spread via interbacterial transfer and the emergence of new variants are important public health concerns. We investigated the molecular properties of SGI1-carryingSalmonella entericaserovars selected from a European strain collection. A total of 38 strains belonging toS. entericaserovar Agona,S. entericaserovar Albany,S. entericaserovar Derby,S. entericaserovar Kentucky,S. entericaserovar Newport,S. entericaserovar Paratyphi B dT+, andS. entericaserovar Typhimurium, isolated between 2002 and 2006 in eight European countries from humans, animals, and food, were subjected to antimicrobial susceptibility testing, molecular typing methods (XbaI pulsed-field gel electrophoresis [PFGE], plasmid analysis, and multilocus variable-number tandem-repeat analysis [MLVA]), as well as detection of resistance and virulence determinants (PCR/sequencing and DNA microarray analysis). Typing experiments revealed wide heterogeneity inside the strain collection and even within serovars. PFGE analysis distinguished a total of 26 different patterns. In contrast, the characterization of the phenotypic and genotypic antimicrobial resistance revealed serovar-specific features. Apart from the classical SGI1 organization found in 61% of the strains, seven different variants were identified with antimicrobial resistance properties associated with SGI1-A (S. Derby), SGI1-C (S. Derby), SGI1-F (S. Albany), SGI1-L (S. Newport), SGI1-K (S. Kentucky), SGI1-M (S.Typhimurium), and, eventually, a novel variant similar to SGI1-C with additional gentamicin resistance encoded byaadB. Only minor serovar-specific differences among virulence patterns were detected. In conclusion, the SGI1 carriers exhibited pathogenetic backgrounds comparable to the ones published for susceptible isolates. However, because of their multidrug resistance, they may be more relevant in clinical settings.

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