Experimental and numerical investigation of T section connections

2021 ◽  
Vol 7 (4) ◽  
pp. 188
Author(s):  
Mahmut Kılıç ◽  
Abdulkadir Cüneyt Aydın ◽  
Merve Sağıroğlu ◽  
Mahyar Maali
Keyword(s):  
Numerical Modeling ◽  
Full Range ◽  
Break Point ◽  
Steel Beam ◽  
Sufficient Information ◽  
T Joints ◽  
Real Behavior ◽  
Rigid Joints ◽  
And Behavior

The paper summarizes recent experimental research on determining the full-range behaviour of steel beam-to-column connections. Unlike the connection types in the literature, numerical modeling was done with various experiments to determine the behavior of two types of connection types. In these joints, T joints have been studied, but unlike the literature, T joint's element is made of plates; It was obtained from 1/2 IPE profile, not by welding. Thus, it is thought that the problems such as workmanship errors, break point formation and in situ welding failures, which occur in the welding of T joints, are eliminated. Necessary studies have been carried out to have sufficient information about the behavior of the T joint to be manufactured from the IPE profile and thus to provide the opportunity for its use. In the light of the data obtained, numerical modeling is done and the torque rotation relation and behavior of semi-rigid joints are numerically modeled. Thus, thanks to the calibrated model with the experiments, the closest results to the real behavior were obtained for the unexamined combinations.

scholarly journals Influence of protein (human galectin-3) design on aspects of lectin activity

2020 ◽  
Vol 154 (2) ◽  
pp. 135-153 ◽  
Author(s):  
Gabriel García Caballero ◽  
Donella Beckwith ◽  
Nadezhda V. Shilova ◽  
Adele Gabba ◽  
Tanja J. Kutzner ◽  
...  
Keyword(s):  
Protein Design ◽  
Full Range ◽  
Biological Information ◽  
The Novel ◽  
Modular Assembly ◽  
Binding Partners ◽  
Galectin 3 ◽  
Potential Applications ◽  
Similar Affinity

Abstract The concept of biomedical significance of the functional pairing between tissue lectins and their glycoconjugate counterreceptors has reached the mainstream of research on the flow of biological information. A major challenge now is to identify the principles of structure–activity relationships that underlie specificity of recognition and the ensuing post-binding processes. Toward this end, we focus on a distinct feature on the side of the lectin, i.e. its architecture to present the carbohydrate recognition domain (CRD). Working with a multifunctional human lectin, i.e. galectin-3, as model, its CRD is used in protein engineering to build variants with different modular assembly. Hereby, it becomes possible to compare activity features of the natural design, i.e. CRD attached to an N-terminal tail, with those of homo- and heterodimers and the tail-free protein. Thermodynamics of binding disaccharides proved full activity of all proteins at very similar affinity. The following glycan array testing revealed maintained preferential contact formation with N-acetyllactosamine oligomers and histo-blood group ABH epitopes irrespective of variant design. The study of carbohydrate-inhibitable binding of the test panel disclosed up to qualitative cell-type-dependent differences in sections of fixed murine epididymis and especially jejunum. By probing topological aspects of binding, the susceptibility to inhibition by a tetravalent glycocluster was markedly different for the wild-type vs the homodimeric variant proteins. The results teach the salient lesson that protein design matters: the type of CRD presentation can have a profound bearing on whether basically suited oligosaccharides, which for example tested positively in an array, will become binding partners in situ. When lectin-glycoconjugate aggregates (lattices) are formed, their structural organization will depend on this parameter. Further testing (ga)lectin variants will thus be instrumental (i) to define the full range of impact of altering protein assembly and (ii) to explain why certain types of design have been favored during the course of evolution, besides opening biomedical perspectives for potential applications of the novel galectin forms.

scholarly journals The SISAL database: a global resource to document oxygen and carbon isotope records from speleothems

2018 ◽  
Author(s):  
Kamolphat Atsawawaranunt ◽  
Laia Comas-Bru ◽  
Sahar Amirnezhad Mozhdehi ◽  
Michael Deininger ◽  
Sandy P. Harrison ◽  
...  
Keyword(s):  
Working Group ◽  
Climate Models ◽  
Full Range ◽  
Global Changes ◽  
Sufficient Information ◽  
Data Set ◽  
Oxygen And Carbon Isotope ◽  
Cave System ◽  
Out Of Sample ◽  
Age Model

Abstract. Stable isotope records from speleothems provide information on past climate changes, most particularly information that can be used to reconstruct past changes in precipitation and atmospheric circulation. These records are increasingly being used to provide “out-of-sample” evaluations of isotope-enabled climate models. SISAL (Speleothem Isotope Synthesis and Analysis) is an international working group of the Past Global Changes (PAGES) project. The working group aims to provide a comprehensive compilation of speleothem isotope records for climate reconstruction and model evaluation. The SISAL database contains data for individual speleothems, grouped by cave system. Stable isotopes of oxygen and carbon (δ18O, δ13C) measurements are referenced by distance from the top or youngest part of the speleothem. Additional tables provide information on dating, including information on the dates used to construct the original age model and sufficient information to assess the quality of each data set and to erect a standardized chronology across different speleothems. The metadata table provides location information, information about the full range of measurements carried out on each speleothem and information about the cave system that is relevant to the interpretation of the records, as well as citations for both publications and archived data. The compiled data are available at http://dx.doi.org/10.17864/1947.139.

Fracture of Metal Foams: In-situ Testing and Numerical Modeling

2004 ◽  
Vol 6 (6) ◽  
pp. 429-431 ◽  
Author(s):  
P.R. Onck ◽  
R. van Merkerk ◽  
J.Th.M. De Hosson ◽  
I. Schmidt
Keyword(s):  
Numerical Modeling ◽  
Metal Foams ◽  
In Situ Testing

The distribution and abundance of halogens in eclogites: An in situ SIMS perspective of the Raspas Complex (Ecuador)

2020 ◽  
Vol 105 (3) ◽  
pp. 307-318 ◽  
Author(s):  
Benjamin M. Urann ◽  
Véronique Le Roux ◽  
Timm John ◽  
Grace M. Beaudoin ◽  
Jaime D. Barnes
Keyword(s):  
Upper Mantle ◽  
Subduction Zones ◽  
Secondary Ion Mass Spectrometry ◽  
Bulk Rock ◽  
Ocean Island Basalts ◽  
High Pressure Metamorphism ◽  
Southern Ecuador ◽  
And Behavior ◽  
Secondary Ion

Abstract We present in situ secondary ion mass spectrometry (SIMS) and electron microprobe analyses of coexisting garnet, omphacite, phengite, amphibole, and apatite, combined with pyrohydrolysis bulk-rock analyses to constrain the distribution, abundance, and behavior of halogens (F and Cl) in six MORB-like eclogites from the Raspas Complex (Southern Ecuador). In all cases concerning lattice-hosted halogens, F compatibility decreases from apatite (1.47–3.25 wt%), to amphibole (563–4727 μg/g), phengite (610–1822 μg/g), omphacite (6.5–54.1 μg/g), and garnet (1.7–8.9 μg/g). The relative compatibility of Cl in the assemblage is greatest for apatite (192–515 μg/g), followed by amphibole (0.64–82.7 μg/g), phengite (1.2–2.1 μg/g), omphacite (<0.05–1.0 μg/g), and garnet (<0.05 μg/g). Congruence between SIMS-reconstructed F bulk abundances and yield-corrected bulk pyrohydrolysis analyses indicates that F is primarily hosted within the crystal lattice of eclogitic minerals. However, SIMS-reconstructed Cl abundances are a factor of five lower, on average, than pyrohydrolysis-derived bulk concentrations. This discrepancy results from the contribution of fluid inclusions, which may host at least 80% of the bulk rock Cl. The combination of SIMS and pyrohydrolysis is highly complementary. Whereas SIMS is well suited to determine bulk F abundances, pyrohydrolysis better quantifies bulk Cl concentrations, which include the contribution of fluid inclusion-hosted Cl. Raspas eclogites contain 145–258 μg/g F and at least 7–11 μg/g Cl. We estimate that ~95% of F is retained in the slab through eclogitization and returned to the upper mantle during subduction, whereas at least 95% of subducted Cl is removed from the rock by the time the slab equilibrates at eclogite facies conditions. Our calculations provide further evidence for the fractionation of F from Cl during high-pressure metamorphism in subduction zones. Although the HIMU (high U/Pb) mantle source (dehydrated oceanic crust) is often associated with enrichments in Cl/K and F/Nd, Raspas eclogites show relatively low halogen ratios identical within uncertainty to depleted MORB mantle (DMM). Thus, the observed halogen enrichments in HIMU ocean island basalts require either further fractionation during mantle processing or recycling of a halogen-enriched carrier lithology such as serpentinite into the mantle.

In situ measurement of advection-dispersion processes in a torrential river, comparison with numerical modeling

2014 ◽  
pp. 221-228 ◽  
Author(s):  
A Hauet ◽  
G Buannic ◽  
G Antoine ◽  
M Jodeau ◽  
M Esteves ◽  
...  
Keyword(s):  
Numerical Modeling ◽  
In Situ Measurement ◽  
Advection Dispersion
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