Component of Variability for Protein Content and Yield Related Traits in Recombinant Inbred Lines (RILs) Population of Bread Wheat (Triticum aestivum L. Em. Thell) in Normal and Late Sown Environments

Grain protein content (GPC) is an important nutritional quality trait of wheat. Aegilops tauschii Coss. is a progenitor of common wheat and has been shown to have high GPC. The objective of this study was to identify quantitative trait loci (QTL) for GPC using A. tauschii–Triticum aestivum L. recombinant lines. An advanced BC2F6 population (112 lines) containing A. tauschii segments was developed using synthetic octaploid wheat (hexaploid wheat Zhoumai 18 × A. tauschii T093), which displayed significant phenotype variances. Two quality traits, GPC and wet gluten, and four yield-related traits, thousand kernel weight, spikelet number per plant, grain number per spike, and grain weight per spike, were evaluated. The results show that the mean GPCs of these lines were significantly higher than those of Zhoumai 18. Correlation and mapping analyses indicated that quality traits were weakly negatively correlated with yield traits. Furthermore, 16 A. tauschii-derived QTL for GPC were detected in the recombinant inbred lines, and four stable QTL that have no significant negative effects on yield and are located within the same marker interval were detected in both environments. Additionally, high-protein, high-yield lines 150228 and 150368 with stable QTL were obtained, and both can be directly utilised for fine mapping of the GPC genes and molecular marker–assisted selection to achieve synergistic improvement of wheat yield and protein content.

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Molecular analysis of YR genes for yellow rust resistance in bread wheat (Triticum aestivum L. Em Thell)

Bangladesh Journal of Botany ◽

10.3329/bjb.v48i4.49076 ◽

2019 ◽

Vol 48 (4) ◽

pp. 1199-1206

Author(s):

Pooja ◽

SS Dhanda ◽

NR Yadav

Keyword(s):

Bread Wheat ◽

Ssr Markers ◽

Recombinant Inbred ◽

Rust Resistance ◽

Yellow Rust ◽

Recombinant Inbred Lines ◽

Triticum Aestivum L ◽

Inbred Lines ◽

Yellow Rust Resistance ◽

Yr Genes

Investigation was conducted to evaluate 210 recombinant inbred lines (RILs) of bread wheat to identify Yr genes using SSR markers and response to stripe rust reaction. Recombinant inbred lines were screened under epiphytotic conditions and data in terms of per cent leaf area infected were recorded using Modified Cobb’s Scale. A total of seven Yr genes, namely Yr7, Yr18, Yr26, Yr29, Yr36, Yr47 and Yr53 were found to be linked to yellow rust resistance. Out of 40 Yr specific SSR markers, seven (Xgwm130 (Yr7), Xbarc 352 (Yr18), Xgwm 11 (Yr26), Xwmc 44 (Yr29), Xwmc 149 (Yr53), WKS1_I (Yr36) and Xcfb309 (Yr47) were found to be polymorphic in parental genotypes and RIL population indicating the presence or absence of Yr genes. Three RILs namely, 51, 52, 55 had largest number of resistant genes (4Yr genes) followed by six lines namely, RIL No. 20, 21, 23, 29, 39, 53 had 3Yr genes having disease score (0S). These RILs may be utilized for incorporation of specific Yr genes in well adapted genotypes for improvement of disease resistance in bread wheat.

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