scholarly journals Stem Analysis of a Pollarded Wild Sugi (Cryptomeria japonica) Tree

2021 ◽  
Vol 27 (0) ◽  
pp. 27-35
Author(s):  
Satoshi Tatsuhara ◽  
Keigo Tanaka ◽  
Koji Yamada ◽  
Hiromi Akashi ◽  
Kimio Takeuchi
Author(s):  
P.E. Batson ◽  
C.R.M. Grovenor ◽  
D.A. Smith ◽  
C. Wong

In this work As doped polysilicon was deposited onto (100) silicon wafers by APCVD at 660°C from a silane-arsine mixture, followed by a ten minute anneal at 1000°C, and in one case a further ten minute anneal at 700°C. Specimens for TEM and STEM analysis were prepared by chemical polishing. The microstructure, which is unchanged by the final 700°C anneal,is shown in Figure 1. It consists of numerous randomly oriented grains many of which contain twins.X-ray analysis was carried out in a VG HB5 STEM. As K α x-ray counts were collected from STEM scans across grain and twin boundaries, Figures 2-4. The incident beam size was about 1.5nm in diameter, and each of the 20 channels in the plots was sampled from a 1.6nm length of the approximately 30nm line scan across the boundary. The bright field image profile along the scanned line was monitored during the analysis to allow correlation between the image and the x-ray signal.


Forests ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 464
Author(s):  
Wenjie Zhang ◽  
Tianzhong Zhao ◽  
Xiaohui Su ◽  
Baoguo Wu ◽  
Zhiqiang Min ◽  
...  

Stem analysis is an essential aspect in forestry investigation and forest management, as it is a primary method to study the growth law of trees. Stem analysis requires measuring the width and number of tree rings to ensure the accurate measurement, expand applicable tree species, and reduce operation cost. This study explores the use of Open Source Computer Vision Library (Open CV) to measure the ring radius of analytic wood disk digital images, and establish a regression equation of ring radius based on image geometric distortion correction. Here, a digital camera was used to photograph the stem disks’ tree rings to obtain digital images. The images were preprocessed with Open CV to measure the disk’s annual ring radius. The error correction model based on the least-square polynomial fitting method was established for digital image geometric distortion correction. Finally, a regression equation for tree ring radius based on the error correction model was established. Through the above steps, click the intersection point between the radius line and each ring to get the pixel distance from the ring to the pith, then the size of ring radius can be calculated by the regression equation of ring radius. The study’s method was used to measure the digital image of the Chinese fir stem disk and compare it with the actual value. The results showed that the maximum error of this method was 0.15 cm, the average error was 0.04 cm, and the average detection accuracy reached 99.34%, which met the requirements for measuring the tree ring radius by stem disk analysis. This method is simple, accurate, and suitable for coniferous and broad-leaved species, which allows researchers to analyze tree ring radius measurement, and is of great significance for analyzing the tree growth process.


Author(s):  
Chi-I Chang ◽  
Cheng-Chi Chen ◽  
Yueh-Hsiung Kuo
Keyword(s):  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoshihiko Nanasato ◽  
Masafumi Mikami ◽  
Norihiro Futamura ◽  
Masaki Endo ◽  
Mitsuru Nishiguchi ◽  
...  

AbstractCryptomeria japonica (Japanese cedar or sugi) is one of the most important coniferous tree species in Japan and breeding programs for this species have been launched since 1950s. Genome editing technology can be used to shorten the breeding period. In this study, we performed targeted mutagenesis using the CRISPR/Cas9 system in C. japonica. First, the CRISPR/Cas9 system was tested using green fluorescent protein (GFP)-expressing transgenic embryogenic tissue lines. Knock-out efficiency of GFP ranged from 3.1 to 41.4% depending on U6 promoters and target sequences. The GFP knock-out region was mottled in many lines, indicating genome editing in individual cells. However, in 101 of 102 mutated individuals (> 99%) from 6 GFP knock-out lines, embryos had a single mutation pattern. Next, we knocked out the endogenous C. japonica magnesium chelatase subunit I (CjChlI) gene using two guide RNA targets. Green, pale green, and albino phenotypes were obtained in the gene-edited cell lines. Sequence analysis revealed random deletions, insertions, and replacements in the target region. Thus, targeted mutagenesis using the CRISPR/Cas9 system can be used to modify the C. japonica genome.


2003 ◽  
Vol 92 (3) ◽  
pp. 291-295 ◽  
Author(s):  
Yukiko Morimoto ◽  
Tadashi Yasuhara ◽  
Akiko Sugimoto ◽  
Atsuko Inoue ◽  
Izumi Hide ◽  
...  
Keyword(s):  

New Forests ◽  
2009 ◽  
Vol 39 (3) ◽  
pp. 261-273 ◽  
Author(s):  
Yoshinari Moriguchi ◽  
Yoko Yamazaki ◽  
Hideaki Taira ◽  
Yoshihiko Tsumura

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