scholarly journals Natural Variation OsCd1V449 Contributes to Reducing Cadmium Accumulation in Rice Grain

2018 ◽  
Author(s):  
Huili Yan ◽  
Wenxiu Xu ◽  
Jianyin Xie ◽  
Yiwei Gao ◽  
Lulu Wu ◽  
...  
Keyword(s):  
Natural Variation ◽  
Genetic Basis ◽  
Genetic Resource ◽  
Cadmium Accumulation ◽  
Rice Grain ◽  
Isogenic Line ◽  
Near Isogenic Line ◽  
Cd Uptake ◽  
Indica Variety ◽  
Grain Cadmium

Cadmium accumulation in rice grain poses a serious threat to people’s health. Understanding the genetic basis on grain cadmium accumulation facilitates efforts to reduce it. Here, we show that OsCd1 is involved in Cd uptake and contributes to grain accumulation in rice. Natural variation in OsCd1 with a missense mutation Val449Asp is responsible for the divergence of rice grain cadmium accumulation between japonica and indica. Notably, near-isogenic line tests confirmed that the indica variety carrying the OsCd1V449 allele could reduce the grain cadmium accumulation. The favorable allele OsCd1V449 may be an important genetic resource to reduce grain cadmium accumulation for indica.

scholarly journals Natural variation in the promoter of OsHMA3 contributes to differential grain cadmium accumulation between Indica and Japonica rice

2020 ◽  
Vol 62 (3) ◽  
pp. 314-329 ◽  
Author(s):  
Chao‐Lei Liu ◽  
Zhen‐Yu Gao ◽  
Lian‐Guang Shang ◽  
Chang‐Hong Yang ◽  
Ban‐Pu Ruan ◽  
...  
Keyword(s):  
Natural Variation ◽  
Cadmium Accumulation ◽  
Japonica Rice ◽  
Grain Cadmium

scholarly journals Quantitative Trait Loci Controlling Light and Hormone Response in Two Accessions ofArabidopsis thaliana

Genetics ◽  
2002 ◽  
Vol 160 (2) ◽  
pp. 683-696 ◽  
Author(s):  
Justin O Borevitz ◽  
Julin N Maloof ◽  
Jason Lutes ◽  
Tsegaye Dabi ◽  
Joanna L Redfern ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Natural Variation ◽  
Red Light ◽  
Light Response ◽  
Environment Interaction ◽  
Isogenic Line ◽  
Hormone Response ◽  
New Genes ◽  
Trait Loci

AbstractWe have mapped quantitative trait loci (QTL) responsible for natural variation in light and hormone response between the Cape Verde Islands (Cvi) and Landsberg erecta (Ler) accessions of Arabidopsis thaliana using recombinant inbred lines (RILs). Hypocotyl length was measured in four light environments: white, blue, red, and far-red light and in the dark. In addition, white light plus gibberellin (GA) and dark plus the brassinosteroid biosynthesis inhibitor brassinazole (BRZ) were used to detect hormone effects. Twelve QTL were identified that map to loci not previously known to affect light response, as well as loci where candidate genes have been identified from known mutations. Some QTL act in all environments while others show genotype-by-environment interaction. A global threshold was established to identify a significant epistatic interaction between two loci that have few main effects of their own. LIGHT1, a major QTL, has been confirmed in a near isogenic line (NIL) and maps to a new locus with effects in all light environments. The erecta mutation can explain the effect of the HYP2 QTL in the blue, BRZ, and dark environments, but not in far-red. LIGHT2, also confirmed in an NIL, has effects in white and red light and shows interaction with GA. The phenotype and map position of LIGHT2 suggest the photoreceptor PHYB as a candidate gene. Natural variation in light and hormone response thus defines both new genes and known genes that control light response in wild accessions.

scholarly journals Lipoxygenase activities during development of root and nodule of soybean

2004 ◽  
Vol 39 (7) ◽  
pp. 625-630 ◽  
Author(s):  
Tatiana Góes Junghans ◽  
Maria Goreti de Almeida Oliveira ◽  
Maurilio Alves Moreira
Keyword(s):  
Growth And Development ◽  
Root Formation ◽  
Lipoxygenase Activity ◽  
Post Inoculation ◽  
Isogenic Line ◽  
Near Isogenic Line ◽  
Near Isogenic Lines ◽  
Isogenic Lines ◽  
Bradyrhizobium Elkanii ◽  
Over Time

The objective of this work was to evaluate root and nodule soybean lipoxygenases in Doko cultivar and in a near isogenic line lacking seed lipoxygenases, inoculated and uninoculated with Bradyrhizobium elkanii. The lipoxygenase activities from roots collected at 3, 5, 9, 13, 18 and 28 days post-inoculation and from nodules collected at 13, 18 and 28 days post-inoculation were measured. The pH-activity profiles from root and nodules suggested that the lipoxygenases pool expressed in these organs from Doko cultivar and triple-null near isogenic lines are similar. The root lipoxygenase activity of Doko and triple-null lines, inoculated and uninoculated, reduced over time. The highest lipoxygenase activity observed at the beginning of root formation suggests the involvement of this enzyme in growth and development of this organ. However, for nodules an expressive increase of lipoxygenase activity was noticed 28 days post-inoculation. Root and nodule showed, at least, two mobility groups for lipoxygenases in immunoblottings, with approximately 94 and 97 kDa.

scholarly journals Genetic Basis Dissection for Eating and Cooking Qualities of Japonica Rice in Northeast China

Agronomy ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 423
Author(s):  
Yaolong Yang ◽  
Xin Xu ◽  
Mengchen Zhang ◽  
Qun Xu ◽  
Yue Feng ◽  
...  
Keyword(s):  
Linkage Disequilibrium ◽  
Northeast China ◽  
Genetic Basis ◽  
Pattern Analysis ◽  
Principal Component ◽  
Rice Grain ◽  
Japonica Rice ◽  
Cooked Rice ◽  
Expression Pattern Analysis ◽  
Gel Consistency

The japonica rice in Northeast China is famous because of its high quality. Eating and cooking qualities (ECQs) are the most important factors that determine cooked rice quality. However, the genetic basis of ECQ of japonica varieties in Northeast China needs further study. In this study, 200 japonica varieties that are widely distributed in Northeast China were collected to evaluate the physicochemical indices of grain ECQs. The distribution of each trait was concentrated without large variations. Correlation analysis indicated that gel consistency (GC) had a significantly negative correlation with gelatinization temperature (GT). By integrating various analyses including kinship calculation, principal component analysis (PCA), linkage disequilibrium (LD) analysis, and original parent investigation, we found that the japonica varieties in Northeast China exhibited a narrow genetic basis. An association study for grain ECQs was performed and eight quantitative trait loci (QTLs) were detected. ALK was the major locus that regulated GT and also significantly affecting GC. Through the linkage disequilibrium (LD) and expression pattern analysis, one possible candidate gene (LOC_Os02g29980) was predicted and required further research for validation. Additionally, a different allele of Wx was identified in the variety CH4126, and ALK was not fixed in these japonica varieties. These results further elucidate the genetic basis of ECQs of japonica varieties in Northeast China and provide local breeders some assistance for improving ECQs of rice grain in rice breeding.

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