scholarly journals Neurogenic Differentiation of Human Dental Pulp Stem Cells on Graphene-Polycaprolactone Hybrid Nanofibers

2018 ◽  
Author(s):  
Hoon Seonwoo ◽  
Kyung-Je Jang ◽  
Dohyeon Lee ◽  
Sunho Park ◽  
Myungchul Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Morphological Characteristics ◽  
Electrospun Nanofibers ◽  
Dental Pulp Stem Cells ◽  
Neurogenic Differentiation ◽  
Dental Tissues ◽  
Differentiated Cells ◽  
Human Dental Pulp

Stem cells derived from dental tissues—dental stem cells—are flavored due to their easy acquisition. Among them, dental pulp stem cells (DPSCs) extracted from the dental pulp have many advantages such as high proliferation and highly purified population. Although their ability for neurogenic differentiation has been highlighted and neurogenic differentiation using electrospun nanofibers (NFs) has been performed, graphene-incorporated NFs have never been applied for DPSC neurogenic differentiation. Here reduced graphene oxide (RGO)-polycaprolactone (PCL) hybrid electrospun NFs were developed and applied for enhanced neurogenesis of DPSCs. First, RGO-PCL NFs were fabricated by electrospinning with incorporation of RGO and alignments, and their chemical and morphological characteristics were evaluated. Furthermore, in vitro NF properties such as influence on the cellular alignments and cell viability of DPSCs were also analyzed. The influences of NFs on DPSCs neurogenesis was also analyzed. The results confirmed that an appropriate concentration of RGO promoted better DPSC neurogenesis. Furthermore, the use of random NFs facilitated contiguous junctions of differentiated cells, whereas the use of aligned NFs facilitated aligned junction of differentiated cells along the direction of NF alignments. Our findings showed that RGO-PCL NFs can be a useful tool for DPSC neurogenesis, which will help regeneration in neurodegenerative and neurodefective diseases.

scholarly journals Neurogenic Differentiation of Human Dental Pulp Stem Cells on Graphene-Polycaprolactone Hybrid Nanofibers

Nanomaterials ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 554 ◽  
Author(s):  
Hoon Seonwoo ◽  
Kyung-Je Jang ◽  
Dohyeon Lee ◽  
Sunho Park ◽  
Myungchul Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Morphological Characteristics ◽  
Electrospun Nanofibers ◽  
Dental Pulp Stem Cells ◽  
Neurogenic Differentiation ◽  
Dental Tissues ◽  
Differentiated Cells ◽  
Human Dental Pulp

Stem cells derived from dental tissues—dental stem cells—are favored due to their easy acquisition. Among them, dental pulp stem cells (DPSCs) extracted from the dental pulp have many advantages, such as high proliferation and a highly purified population. Although their ability for neurogenic differentiation has been highlighted and neurogenic differentiation using electrospun nanofibers (NFs) has been performed, graphene-incorporated NFs have never been applied for DPSC neurogenic differentiation. Here, reduced graphene oxide (RGO)-polycaprolactone (PCL) hybrid electrospun NFs were developed and applied for enhanced neurogenesis of DPSCs. First, RGO-PCL NFs were fabricated by electrospinning with incorporation of RGO and alignments, and their chemical and morphological characteristics were evaluated. Furthermore, in vitro NF properties, such as influence on the cellular alignments and cell viability of DPSCs, were also analyzed. The influences of NFs on DPSCs neurogenesis were also analyzed. The results confirmed that an appropriate concentration of RGO promoted better DPSC neurogenesis. Furthermore, the use of random NFs facilitated contiguous junctions of differentiated cells, whereas the use of aligned NFs facilitated an aligned junction of differentiated cells along the direction of NF alignments. Our findings showed that RGO-PCL NFs can be a useful tool for DPSC neurogenesis, which will help regeneration in neurodegenerative and neurodefective diseases.

scholarly journals Novel Molecule Nell-1 Promotes the Angiogenic Differentiation of Dental Pulp Stem Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Mengyue Li ◽  
Qiang Wang ◽  
Qi Han ◽  
Jiameng Wu ◽  
Hongfan Zhu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Umbilical Vein ◽  
Dental Pulp Stem Cells ◽  
Exposure Model ◽  
Dental Tissues ◽  
Normal Rat ◽  
Pulp Exposure ◽  
Angiogenic Markers

IntroductionThis work aimed to reveal the crucial role of Nell-1 in the angiogenic differentiation of human dental pulp stem cells (DPSCs) alone or co-cultured with human umbilical vein endothelial cell (HUVECs) in vitro and whether this molecule is involved in the pulp exposure model in vivo.MethodsImmunofluorescence was conducted to ascertain the location of Nell-1 on DPSCs, HUVECs, and normal rat dental tissues. RT-PCR, Western blot, and ELISA were performed to observe the expression levels of angiogenic markers and determine the angiogenic differentiation of Nell-1 on DPSCs alone or co-cultured with HUVECs, as well as in vitro tube formation assay. Blood vessel number for all groups was observed and compared using immunohistochemistry by establishing a rat pulp exposure model.ResultsNell-1 is highly expressed in the nucleus of DPSCs and HUVECs and is co-expressed with angiogenic markers in normal rat pulp tissues. Hence, Nell-1 can promote the angiogenic marker expression in DPSCs alone and co-cultured with other cells and can enhance angiogenesis in vitro as well as in the pulp exposure model.ConclusionNell-1 may play a positive role in the angiogenic differentiation of DPSCs.

An In Vitro Study of the Effects of Crocin on the Modulation of DSPP, VEGF-A, HLA-G5, STAT3 and CD200 Expression in Human Dental Pulp Stem Cells

2021 ◽  
Author(s):  
Mansoore Saharkhiz ◽  
Fariba Emadian Razavi ◽  
Seyed Mohammad Riahi ◽  
Malaksima Ayadilord ◽  
Zeinab Rostami ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
In Vitro Study ◽  
Dental Pulp Stem Cells ◽  
Vitro Study ◽  
Human Dental Pulp

scholarly journals Regenerative potential of human dental pulp stem cells in the treatment of stress urinary incontinence: In vitro and in vivo study

2019 ◽  
Vol 52 (6) ◽  
Author(s):  
Alessio Zordani ◽  
Alessandra Pisciotta ◽  
Laura Bertoni ◽  
Giulia Bertani ◽  
Antonio Vallarola ◽  
...  
Keyword(s):  
Stem Cells ◽  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
In Vivo Study ◽  
Human Dental Pulp

scholarly journals In vitro induction of odontogenic activity of human dental pulp stem cells by white Portland cement enriched with zirconium oxide and zinc oxide components

2019 ◽  
Vol 13 (1) ◽  
pp. 3-10 ◽  
Author(s):  
Saeed Rahimi ◽  
Sadegh Salarinasab ◽  
Negin Ghasemi ◽  
Reza Rahbarghazi ◽  
Shahriar Shahi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Zirconium Oxide ◽  
Dental Pulp Stem Cells ◽  
Ion Release ◽  
Zno Nps ◽  
Alp Activity ◽  
Human Dental Pulp ◽  
White Portland Cement

Background. The aim of this in vitro study was to investigate the effect of zinc oxide (ZnO) and zirconium oxide (ZrO2) microparticles (MPs) and nanoparticles (NPs) in combination with white Portland cement (WPC) on odontogenic capacity of human dental pulp stem cells over a period of 21 days. Methods. Synthesized ZnO and ZrO2 particles were characterized using scanning electron microscopy and transmission electron microscopy. The viability of human dental pulp stem cells was measured by a 3-(4,5-dimethylthiazolyl-2-yl)-2,5- diphenyltetrazolium bromide assay at 7-, 14- and 21-day intervals after seeding on WPC disks enriched with ZnO and ZrO2 MPs and NPs. Odontogenic potential of ZnO and ZrO2 particles in combination with WPC was investigated by alkaline phosphatase (ALP) activity and ionized calcium level of supernatant culture media at different time intervals. Data were analyzed using one-way ANOVA and post hoc Tukey tests. Results. All the materials exhibited cell viability over a 21-day period, except for WPC with ZnO NPs on day 7, although it was not statistically significant (P>0.05). The ALP activity and ionized calcium level increased in all the groups compared to the control group (P<0.05). ZnO NPs had superior effect on odontogenic activity and calcium ion release compared to ZnO MPs (P=0.046). There was no significant difference between ZrO2 MPs and NPs in odontogenic activity (P>0.05). Conclusion. WPC enriched with ZnO and ZrO2 increased ALP activity and calcium ion release of human dental pulp stem cells over a period of 21 days in vitro.

In vitro biocompatibility of ICON® and TEGDMA on human dental pulp stem cells

2016 ◽  
Vol 32 (8) ◽  
pp. 1052-1064 ◽  
Author(s):  
Lina Gölz ◽  
Ruth Andrea Simonis ◽  
Joana Reichelt ◽  
Helmut Stark ◽  
Matthias Frentzen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
In Vitro Biocompatibility ◽  
Human Dental Pulp

scholarly journals Biological Characteristics of Fluorescent Superparamagnetic Iron Oxide Labeled Human Dental Pulp Stem Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Liang Ma ◽  
Ming-wei Li ◽  
Yu Bai ◽  
Hui-hui Guo ◽  
Sheng-chao Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Iron Oxide ◽  
Dental Pulp ◽  
Superparamagnetic Iron Oxide ◽  
Biological Properties ◽  
Dental Pulp Stem Cells ◽  
Iron Oxide Particles ◽  
Human Dental Pulp

Tracking transplanted stem cells is necessary to clarify cellular properties and improve transplantation success. In this study, we investigate the effects of fluorescent superparamagnetic iron oxide particles (SPIO) (Molday ION Rhodamine-B™, MIRB) on biological properties of human dental pulp stem cells (hDPSCs) and monitor hDPSCs in vitro and in vivo using magnetic resonance imaging (MRI). Morphological analysis showed that intracellular MIRB particles were distributed in the cytoplasm surrounding the nuclei of hDPSCs. 12.5–100 μg/mL MIRB all resulted in 100% labeling efficiency. MTT showed that 12.5–50 μg/mL MIRB could promote cell proliferation and MIRB over 100 μg/mL exhibited toxic effect on hDPSCs. In vitro MRI showed that 1 × 106cells labeled with various concentrations of MIRB (12.5–100 μg/mL) could be visualized. In vivo MRI showed that transplanted cells could be clearly visualized up to 60 days after transplantation. These results suggest that 12.5–50 μg/mL MIRB is a safe range for labeling hDPSCs. MIRB labeled hDPSCs cell can be visualized by MRI in vitro and in vivo. These data demonstrate that MIRB is a promising candidate for hDPSCs tracking in hDPSCs based dental pulp regeneration therapy.

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