scholarly journals Root System Architecture, Copper Uptake and Tissue Distribution in Soybean (Glycine max cv. Kowsar) Grown in Copper Oxide Nanoparticles (CuONPs) Amended Soil and Implications to Human Nutrition

2020 ◽  
Author(s):  
Elham Yusefi-Tanha ◽  
Sina Fallah ◽  
Ali Rostamnejadi ◽  
Lok Pokhrel
Keyword(s):  
Particle Size ◽  
System Architecture ◽  
Root Architecture ◽  
Soybean Seed ◽  
Copper Oxide Nanoparticles ◽  
Root System Architecture ◽  
Oxide Nanoparticles ◽  
Total Seed ◽  
Cu Uptake ◽  
Stem Leaf

Understanding potential uptake and biodistribution of engineered nanoparticles in soil-grown plants is imperative for toxicity and risk assessment considering the oral exposure of edibles by humans. Herein, we assessed potential influence of particle size (25, 50, and 250 nm) and concentration (0, 50, 100, 200, and 500 mg/kg-soil) of Copper oxide nanoparticles (CuONPs) on: (1) the root system architecture, and the physicochemical attributes of soil at the soil-root interface, (2) leading to Cu transport and accumulation in root, stem, leaf and seed in soybean (Glycine max cv Kowsar) grown for entire lifecycle of 120 days, and compared with soluble Cu2+ ions and water-only controls, and (3) performed a comparative assessment of total seed Cu levels in soybean with other valuable food sources for Cu intake and discussed its human health implications. Our findings showed particle size- and concentration-dependent influence of CuONPs on Cu uptake and tissue distribution in root, stem, leaf and seed in soybean. Alterations in root architecture (root dry weight, root length, root volume, and root area) were dependent on the Cu compound type, Cu concentrations, and their interactions (p<0.05), except for root density. Concentration-response relationships for all three sized CuONPs, and Cu2+ ions, were linear. CuONPs and Cu2+ ions had inhibitory effects on root growth and development. Overall, soybean responses to smallest size CuONPs-25 nm were higher for all parameters investigated compared to two larger sized CuONPs (50 nm, 250 nm) or Cu2+ ions. Cu uptake/bioaccumulation differed among soybean tissues in the order: root > leaf > stem > seed. Despite reduced root architecture and seed yield, our smallest size CuONPs-25 nm led to increased total seed Cu uptake compared to the larger sized CuONPs and Cu2+ ions tested. Our findings also suggest that soil amendment by CuONPs, more so by the smallest size CuONPs-25 nm, could significantly improve nutritional Cu value in soybean seed as reflected by % Daily Values (DV), and are rated “Good” to “Very Good” according to the “World’s Healthiest Foods” rating. However, until the potential toxicity and risk from consumption of soybean seed is characterized in humans, caution should be exercised when the Cu fortified seeds are used for daily human consumption when addressing Cu deficiency and associated illnesses, globally.

scholarly journals Root System Architecture, Copper Uptake and Tissue Distribution in Soybean (Glycine max (L.) Merr.) Grown in Copper Oxide Nanoparticle (CuONP)-Amended Soil and Implications for Human Nutrition

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1326
Author(s):  
Elham Yusefi-Tanha ◽  
Sina Fallah ◽  
Ali Rostamnejadi ◽  
Lok Raj Pokhrel
Keyword(s):  
Particle Size ◽  
Glycine Max ◽  
Copper Oxide ◽  
System Architecture ◽  
Root Architecture ◽  
Root System Architecture ◽  
Glycine Max L ◽  
Total Seed ◽  
Cu Uptake ◽  
Stem Leaf

Understanding the potential uptake and biodistribution of engineered nanoparticles (ENPs) in soil-grown plants is imperative for realistic toxicity and risk assessment considering the oral intake of edibles by humans. Herein, growing N-fixing symbiont (Bradyrhizobium japonicum) inoculated soybean (Glycine max (L.) Merr.) for a full lifecycle of 120 days, we assessed the potential influence of particle size (25, 50, and 250 nm) and concentration (0, 50, 100, 200, and 500 mg/kg soil) of Copper oxide nanoparticles (CuONPs) on: (1) root system architecture, (2) soil physicochemical attributes at the soil–root interface, and (3) Cu transport and accumulation in root, stem, leaf, and seed in soybean, and compared them with the soluble Cu2+ ions and water-only controls. Finally, we performed a comparative assessment of total seed Cu levels in soybean with other valuable food sources for Cu intake and discussed potential human health implications. Results showed particle size- and concentration-dependent influence of CuONPs on Cu uptake and distribution in root, stem, leaf, and seed. Alterations in root architecture (root biomass, length, volume, and area) were dependent on the Cu compound types, Cu concentrations, and their interactions. Concentration–response relationships for all three sizes of CuONPs and Cu2+ ions were found to be linear. Furthermore, CuONPs and Cu2+ ions had inhibitory effects on root growth and development. Overall, soybean responses to the smallest size of CuONPs–25 nm—were greater for all parameters tested compared to the two larger-sized CuONPs (50 nm, 250 nm) or Cu2+ ions. Results suggest that minor changes in soil-root physicochemical attributes may not be a major driver for Cu uptake in soybean. Cu bioaccumulation followed the order: root > leaf > stem > seed. Despite reduction in root architecture and seed yield, the smallest size CuONPs–25 nm led to increased total seed Cu uptake compared to the larger-sized CuONPs or Cu2+ ions. Our findings also suggest that soil amendment with CuONPs, and more so with the smallest size of CuONPs–25 nm—could significantly improve seed nutritional Cu value in soybean as reflected by the % Daily Values (DV) and are rated “Good” to “Very Good” according to the “World’s Healthiest Foods” rating. However, until the potential toxicity and risk from CuONP-fortified soybean seed ingestion is characterized in humans, we caution recommending such seeds for daily human consumption when addressing food Cu-deficiency and associated diseases, globally.

scholarly journals Effective adsorptive removal of azodyes on synthesized copper oxide nanoparticles

2020 ◽  
Vol 10 (3) ◽  
pp. 5369-5375 ◽  
Keyword(s):  
Particle Size ◽  
Copper Oxide ◽  
Camellia Sinensis ◽  
Particle Shape ◽  
Copper Ions ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Uv Spectrum ◽  
Tetragonal Particle ◽  
Phase And Chemical Composition

Copper oxide nanoparticles were synthesized by using Camellia Sinensis leaves extract as a reducing and capping agent of the copper ions in solutions and its azodyes adsorptive efficiency were studied. The produced copper oxide nanoparticles were subsequently characterized by SEM, TEM, XRD, FTIR, and UV spectrophotometer for investigating its particle shape, size, crystalline phase and chemical composition. The particle size of the prepared copper oxide nanoparticle was calculated from the XRD data by using the Scherrer equation was found 17.26 nm. However, the median particle size calculated from the SEM and TEM image analysis was found 25~85 nm of tetragonal particle shape. UV spectrum was obtained with maximum absorption peak at 280 nm. The FTIR spectrum indicated -OH, -C=C- and -C-H functional groups, which is due to the presence of the stabilized layer of the Camellia Sinensis leaf extract which is binded with the prepared copper oxide nanoparticles. The produced copper oxide nanoparticles were used for studying the degradation of Congo red and Malachite green azodies. Different parameters were studied to optimize the reaction conditions. Kinetic models of Langmuir, Freundlich and Elovich models were also applied. The degradation percent of the investigated azodyes on the surface of the produced copper oxide nanoparticles in aqueous solutions was observed between 70-75%.

scholarly journals Green synthesis of copper oxide nanoparticles using Juglans regia leaf extract and assessment of their physico-chemical and biological properties

2019 ◽  
Vol 8 (1) ◽  
pp. 557-567 ◽  
Author(s):  
Marjan Asemani ◽  
Navideh Anarjan
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Copper Oxide ◽  
Leaf Extract ◽  
Copper Oxide Nanoparticles ◽  
Copper Salt ◽  
Oxide Nanoparticles ◽  
Furnace Temperature ◽  
Cuo Nps ◽  
Synthesis Parameters

Abstract Copper oxide nanoparticles (CuO NPs) were green synthesized using walnut leaf extract. Effects of three synthesis parameters namely; amount copper salt (1-4 g), amount of walnut leaf extract (10-40 mL) and furnace temperature (300-500°C), on the particle size as manifested in broad absorption peak (λmax, nm), concentration (absorbance), antioxidant activity and antibacterial activity as minimum inhibitory concentration (MIC) of the fabricated CuO NPs were studied using response surface methodology, based on Box behnken experimental design. The spherical and crystalline monodispersed fabricated CuO NPs with mean particle size of 80 nm, were achieved using optimum synthesis parameters including 1 g copper salt, 14 mL walnut leaf extract and 490°C of furnace temperature. The fabricated CuO NPs at these conditions had maximum antioxidant activity of 83.64% and minimum MIC value of 1.78% w/v against E. coli, with λmax and absorbance values of 226 nm, 4.44% a.u., respectively.

Relative Contributions of Copper Oxide Nanoparticles and Dissolved Copper to Cu Uptake Kinetics of Gulf Killifish (Fundulus grandis) Embryos

2017 ◽  
Vol 51 (3) ◽  
pp. 1395-1404 ◽  
Author(s):  
Chuanjia Jiang ◽  
Benjamin T. Castellon ◽  
Cole W. Matson ◽  
George R. Aiken ◽  
Heileen Hsu-Kim
Keyword(s):  
Copper Oxide ◽  
Copper Oxide Nanoparticles ◽  
Uptake Kinetics ◽  
Oxide Nanoparticles ◽  
Fundulus Grandis ◽  
Kinetics Of ◽  
Cu Uptake

ANTIBIOTIC PROFILE AND ANTIBACTERIAL ACTIVITY OF COPPER OXIDE NANOPARTICLES AGAINST Pseudomonas fluorescens ISOLATED FROM WOUND INFECTION

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Haider Qassim Raheem ◽  
Takwa S. Al-meamar ◽  
Anas M. Almamoori
Keyword(s):  
Antibacterial Activity ◽  
Copper Oxide ◽  
Pseudomonas Fluorescens ◽  
Wide Spectrum ◽  
Copper Oxide Nanoparticles ◽  
Morphological Properties ◽  
Clinical Samples ◽  
Oxide Nanoparticles ◽  
Cuo Nps ◽  
Disk Diffusion Assay

Fifty specimens were collected from wound patients who visited Al-Hilla Teaching Hospital. The samples were grown on Blood and MacConkey agar for 24-48 hr at 37oC. The bacterial isolates which achieved as a pure and predominant growth from clinical samples as Pseudomonas fluorescens, were identified using morphological properties and Vitek2 system. The anti-bacterial activity of copper oxide nanoparticles (CuO NPs) against was tested by (disk diffusion assay) using dilutions of (400, 200, 100, 50, 25, and 12.5‎µ‎g/ml). The (MIC and MBC) of each isolate was determined. CuO NPs shows wide spectrum antibacterial activity against tested bacteria with rise zone of inhibition diameter that is proportionate with the increase in nanoparticle concentration. The MIC of CuO NPs extended from 100-200‎µ‎g/ml and the MBC ranged from 200-400‎µ‎g/ml. The antibiotic profile was determined by Viteck 2 compact system (Biomérieux). CuO NPs‎ found highly effective and safe in P. fluorescens wounds infections comparing with used antibiotics.

Green synthesis of copper oxide nanoparticles using aloe vera extract

2020 ◽  
Vol 3 (3) ◽  
Author(s):  
Hemalatha D ◽  
Saraswath S
Keyword(s):  
Copper Oxide ◽  
Biomedical Application ◽  
Aloe Vera ◽  
Copper Oxide Nanoparticles ◽  
Klebsiella Pneumonia ◽  
Oxide Nanoparticles ◽  
Cupric Sulfate ◽  
X Ray Diffraction ◽  
Fouriertransform Infrared Spectroscopy ◽  
Effective Use

In material science, green method for synthesis of nanomaterials is feasible, cheaper and eco-friendly protocol. To accomplish this phenomenon, present study was aimed to synthesize Copper oxide nanoparticles using leaf extract of Aloevera with two different precursors CuCl2.2H2O (Cupric chloride) and CuSo4.5H2O (Cupric sulfate). The extraction of Aloevera is employed as reducing and stabilizing agent for this synthesis.Copper oxide Nanoparticles is effective use of biomedical application due to their antibacterial function. The synthesized Copper oxide nanoparticles were characterized by X-Ray Diffraction Spectroscopy (XRD), Energy Dispersive Spectroscopy (EDX), FourierTransform Infrared Spectroscopy (FT- IR) and Scanning Electron Microscope(SEM). XRD studies reveal the crystallographic nature of Copper oxide nanoparticles. Furthermore the Copper oxide nanoparticles have good Antibacterial activity against both gram negative (E.Coli, Klebsiella pneumonia) and gram positive (Bacillus cereus, Staphylococcus aureus)bacteria.

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