scholarly journals Human Tendon Repair Following Injection of Autologous, Unmodified Stem Cells: A Comprehensive Immunohistochemical Evaluation

2020 ◽  
Author(s):  
Eckhard Alt ◽  
Ralf Rothoerl ◽  
Matthias Hoppert ◽  
Hans-Georg Frank ◽  
Christopher Alt ◽  
...  
Keyword(s):  
Stem Cells ◽  
Rotator Cuff ◽  
Adult Stem Cells ◽  
Treatment Options ◽  
Tendon Repair ◽  
Immunohistochemical Analysis ◽  
Supraspinatus Tendon ◽  
Tendon Tissue ◽  
Human Tendon ◽  
Regenerative Cells

Current clinical treatment options for symptomatic rotator cuff tear offer only limited potential for true tissue healing and improvement of clinical results. In animal models, injections of adult stem cells isolated from adipose tissue into tendon injuries evidenced histological regeneration of tendon tissue. However, it is unclear whether such beneficial effects could also be observed in a human tendon treated with autologous, adipose derived regenerative cells. Here we demonstrate, for the first time, a comprehensive histological and immunohistochemical analysis of the biopsy of a supraspinatus tendon of a 66-year-old subject with traumatic rotator cuff injury, taken ten weeks after local injection of fresh, uncultured, autologous, adipose derived regenerative cells (UA-ADRCs), prepared at the point of care. Our analysis demonstrated clear evidence towards regenerative healing of the injured supraspinatus tendon. Of note, no formation of adipocytes was observed. These findings indicate that injected autologous, unmodified stem cells can indeed form new tendon tissue and regenerate an injured human tendon.

scholarly journals Current treatment concepts of calcifying tendinitis of the shoulder

2021 ◽  
Author(s):  
Markus Loew ◽  
Marc Schnetzke ◽  
Sven Lichtenberg
Keyword(s):  
Rotator Cuff ◽  
Treatment Options ◽  
Tendon Repair ◽  
Supraspinatus Tendon ◽  
Current Treatment ◽  
Treatment Recommendation ◽  
Calcifying Tendinitis ◽  
Therapeutic Concepts ◽  
Shoulder Region ◽  
Treatment Concepts

AbstractCalcifying tendinitis is a very common pathology in the shoulder region, which may be acute or chronic. The rotator cuff, particularly the supraspinatus tendon, is most frequently affected. The tendons of the rotator cuff undergo a natural cycle of destruction and regeneration. The development of calcifying tendinitis is caused by defective tendon repair. Typical is a chronic course with acute pain exacerbation, which leads to medical presentation. Treatment options range from conservative therapy including rest, physical therapy, nonsteroidal anti-inflammatory drugs, and shock wave therapy, to surgical therapy with arthroscopic removal of the calcific focus. This article discusses the current stage-dependent therapeutic concepts and provides a treatment recommendation.

scholarly journals First Immunohistochemical Evidence of Human Tendon Repair Following Stem Cell Injection: A Case Report and Review of Literature

2021 ◽  
Author(s):  
Eckhard Alt ◽  
Ralf Rothoerl ◽  
Matthias Hoppert ◽  
Hans-Georg Frank ◽  
Tobias Wuerfel ◽  
...  
Keyword(s):  
Adult Stem Cells ◽  
Treatment Options ◽  
Tendon Repair ◽  
Microscopic Analysis ◽  
Clinical Results ◽  
Beneficial Effects ◽  
Tissue Healing ◽  
Human Tendon ◽  
Stem Cell Injection ◽  
Immunohistochemical Evidence

Current clinical treatment options for symptomatic, partial-thickness rotator cuff tear (sPTRCT) offer only limited potential for true tissue healing and improvement of clinical results. In animal models, injections of adult stem cells isolated from adipose tissue into tendon injuries evidenced histological regeneration of tendon tissue. However, it is unclear whether such beneficial effects could also be observed in a human tendon treated with fresh, uncultured, autologous, adipose derived regenerative cells (UA-ADRCs). A specific challenge in this regard is that UA-ADRCs cannot be labeled and, thus, not unequivocally identified in the host tissue. Therefore, histological regeneration of injured human tendons after injection of UA-ADRCs must be assessed using comprehensive, immunohistochemical and microscopic analysis of biopsies taken from the treated tendon a few weeks after injection of UA-ADRCs.

scholarly journals Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Pietro Randelli ◽  
Alessandra Menon ◽  
Vincenza Ragone ◽  
Pasquale Creo ◽  
Sonia Bergante ◽  
...  
Keyword(s):  
Stem Cells ◽  
Rotator Cuff ◽  
Healing Process ◽  
Cost Effective ◽  
Proliferation Rate ◽  
Stem Cell Marker ◽  
Bone Marrow Biopsies ◽  
Resident Stem Cells ◽  
Tendon Stem Cells ◽  
Human Tendon

Increasing the success rate of rotator cuff healing remains tremendous challenge. Among many approaches, the possibility of activating resident stem cells in situ, without the need to isolate them from biopsies, could represent valuable therapeutic strategy. Along this line, it has been recently demonstrated that lipoaspirate product, Lipogems, contains and produces growth-factors that may activate resident stem cells. In this study, human tendon stem cells (hTSCs) from the rotator cuff were cocultured in a transwell system with the Lipogems lipoaspirate product and compared to control untreated cells in terms of cell proliferation, morphology, stem cell marker and VEGF expression, and differentiation and migration capabilities. Results showed that the Lipogems product significantly increases the proliferation rate of hTSCs without altering their stemness and differentiation capability. Moreover, treated cells increase the expression of VEGF, which is crucial for the neovascularization of the tissue during the healing process. Overall, this study supports that directly activating hTSCs with the Lipogems lipoaspirate could represent a new practical therapeutic approach. In fact, obtaining a lipoaspirate is easier, safer, and more cost-effective than harvesting cells from tendon or bone marrow biopsies, expanding them in GMP facility and then reinjecting them in the patient.

scholarly journals Does the Interval Slide Procedure Reduce Supraspinatus Tendon Repair Tension?: A Biomechanical Cadaveric Study

2022 ◽  
Vol 10 (1) ◽  
pp. 232596712110668
Author(s):  
Felix Porschke ◽  
Philip Christian Nolte ◽  
Christian Knye ◽  
Christel Weiss ◽  
Stefan Studier-Fischer ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Intervention Group ◽  
Tendon Repair ◽  
Supraspinatus Tendon ◽  
Rotator Cuff Tears ◽  
Control Group ◽  
Full Thickness ◽  
Time Points ◽  
Tendon Tears ◽  
And Control

Background: The benefits of the interval slide (IS) procedure in retracted rotator cuff tears remain controversial. Purpose: The purpose was to evaluate the effect of the IS procedure on repair tension (RT). It was hypothesized that the IS procedure (anterior IS [AIS], posterior IS [PIS], and intra-articular capsular release [CR]) would reduce the RT of a supraspinatus tendon. Study Design: Controlled laboratory study. Methods: A total of 31 Thiel-embalmed human cadaveric shoulders (mean age, 74 years; range, 68-84 years) were tested. Full-thickness supraspinatus tendon tears were created, and 1 cm of tendon was resected to simulate a retracted defect. Shoulders were randomized into intervention (n = 16) and control (n = 15) groups. In all shoulders, the load during tendon reduction to footprint was measured, an endpoint was defined as maximum tendon lateralization before 50 N was reached, and the RT (load during lateralization to endpoint) of the native tendon (t1) was evaluated. In the intervention group, AIS (t2), PIS (t3), and CR (t4) were performed in order, with RT measurement after each step. In the control group, RT was assessed at the same time points without the intervention. Results: A complete reduction of the tendon was not achieved in any of the shoulders. Mean maximum lateralization was 6.7 ± 1.30 mm, with no significant differences between groups. In the intervention group, the overall IS procedure reduced RT about 47.0% (t1 vs t4: 38.7 ± 3.9 vs 20.5 ± 12.3 N; P < .001). AIS reduced RT significantly (t1 vs t2: 38.7 ± 3.9 vs 27.4 ± 10.5 N; P < .001), whereas subsequent PIS (t2 vs t3: 27.4 ± 10.5 vs 23.2 ± 12.4 N; P = .27) and CR (t3 vs t4: 23.2 ± 12.4 vs 20.5 ± 12.3 N; P = .655) did not additionally reduce tension. Comparison between groups at t4 revealed a reduction of RT of about 47.8% (control vs intervention: 39.3 ± 4.0 vs 20.5 ± 12.3 N; P < .001). Conclusion: The IS procedure reduces RT of the supraspinatus tendon in human cadaveric shoulders. However, performing PIS and CR subsequent to AIS does not reduce tension additionally. Clinical Relevance: These findings provide surgeons with a biomechanical rationale regarding the efficacy of the IS procedure.

scholarly journals Stem Cells: Prospects and Potential Applications in Tanzania: A review

2020 ◽  
Vol 21 (2) ◽  
pp. 1-8
Author(s):  
Afadhali Denis Russa
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Large Scale ◽  
Adult Stem Cells ◽  
Treatment Options ◽  
Stem Cell Technology ◽  
African Countries ◽  
Cell Technology ◽  
Leading Role

Stem cell technology and its application in regenerative medicine is the future gateway for the treatment of most non-communicable diseases (NCDs). As the burden of NCDs continues to rises globally, regenerating the cells, tissues and organs will be the mainstream treatment option. The world is prepared for this intriguing but promising avenue of biomedical technology and medicine but Africa is grossly lagging far behind. African governments, universities, research and health institutions need to take a leading role in empowering and mainstreaming stem cell research.  Moreover, for Africa, there is a huge potential for translating stem cell technology into clinical treatments due to the fact that there are limited treatment options for life-threatening forms of NCDs.  Some African countries have well-developed stem cell facilities and large-scale stem cell therapy centers. The use of adult stem cells in liver failure, diabetes and cardiac infarcts has shown success in some African countries. The present work reviews the status, potential and future prospects of stem cell technology and regenerative medicine in Tanzania with particular emphasis on the adult stem cells applicability into the immediate use inpatient care.  The paper also reviews the available cell identification systems and markers and moral and ethical aspects of stem cell science necessary in the translational treatment regimens. 

Ligament and Tendon Repair with Adult Stem Cells

Stem Cells ◽  
2005 ◽  
pp. 497-509 ◽  
Author(s):  
James Cho Hong Goh ◽  
Hong Wei Ouyang
Keyword(s):  
Stem Cells ◽  
Adult Stem Cells ◽  
Tendon Repair

Ex-vivo Tendon Repair Augmented with Bone Marrow Derived Mesenchymal Stem Cells Stimulated with Myostatin for Tenogenesis

2018 ◽  
Vol 23 (01) ◽  
pp. 47-57 ◽  
Author(s):  
Wei Le ◽  
Andre Eu-Jin Cheah ◽  
Jeffrey Yao
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Upper Limb ◽  
Ex Vivo ◽  
Tendon Repair ◽  
Tendon Tissue ◽  
Flexor Tendons ◽  
Stimulation Of

Background: To investigate the effect of myostatin (GDF-8) stimulation of bone marrow derived mesenchymal stem cells (BMSCs) on tenogenesis in the setting of tendon repair. GDF-8 has demonstrated the ability to augment tenogenesis and we sought to identify if this effect could lead to the focused differentiation of pluripotential stem cells down a tenocyte lineage ex vivo.Methods: Cadaveric upper limb flexor tendons were harvested, decellularized and divided into 1 cm segments. Sutures seeded with stem cells were passed through tendon segments to simulate repair. The repaired tendons were then cultured either with or without myostatin for 3, 5, and 7 days. The experiment was also repeated with non-decellularized tendons for a total of 4 groups. The tendons were then evaluated for the expression of scleraxis and tenomodulin, two biomarkers for tendon.Results: Myostatin stimulation led to an increase in expression of tenomodulin and scleraxis at 5 and 7 days in both the decellularized and non-decellularized tendons. Myostatin increased the differentiation of BMSCs into tenocytes and/or led to the upregulation of tenomodulin and scleraxis production by the native tenocytes present within the non-decellularized tendons.Conclusions: The addition of myostatin to BMSCs leads to tenocyte differentiation as evidenced by the expression of tenocyte biomarkers, scleraxis and tenomodulin. This effect is maintained in an ex vivo tendon repair model suggestive that these cells survive the passage through tendon tissue and remain metabolically active.

Ligament and Tendon Repair with Adult Stem Cells

Stem Cells ◽  
2010 ◽  
pp. 503-522
Author(s):  
Sambit Sahoo ◽  
Hong Wei Ouyang ◽  
James Cho Hong Goh
Keyword(s):  
Stem Cells ◽  
Adult Stem Cells ◽  
Tendon Repair
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