scholarly journals Sex Differences in Photoprotective Responses to 1,25- Dihydroxyvitamin D3 in Mice are Modulated by the Estrogen Receptor-β

2020 ◽  
Author(s):  
Wannit Tongkao-on ◽  
Chen Yang ◽  
Bianca Y McCarthy ◽  
Warusavithana GM De Silva ◽  
Mark S Rybchyn ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptor Beta ◽  
Contact Hypersensitivity ◽  
Cyclobutane Pyrimidine Dimers ◽  
Dihydroxyvitamin D3 ◽  
Female Mice ◽  
1,25 Dihydroxyvitamin D3 ◽  
Pyrimidine Dimers ◽  
Uv Induction ◽  
Receptor Beta

Susceptibility to photoimmune suppression and photocarcinogenesis is greater in male than in female humans and mice and is exacerbated in female estrogen receptor-beta knockout (ER-β-/-) mice. We previously reported that the active vitamin D hormone, 1,25-dihydroxyvitamin D3 (1,25(OH)2D) applied topically protects against ultraviolet radiation (UV)-induction of cutaneous cyclobutane pyrimidine dimers (CPDs) and suppression of contact hypersensitivity (CHS) in female mice. Here we compare these responses in female versus male Skh:hr1 mice, in ER-β-/- versus wild type C57BL/6 mice, and in female ER-blockaded Skh:hr1 mice. Induction of CPDs was significantly greater in male than female Skh:hr1 mice and was more effectively reduced by 1,25(OH)2D in female Skh:hr1 and C57BL/6 mice, than in male Skh:hr1 or ER-β-/- mice respectively. This correlated with reduced sunburn inflammation by 1,25(OH)2D in female but not male Skh:hr1 mice. Furthermore, although 1,25(OH)2D alone dose-dependently suppressed basal CHS responses in male Skh:hr1 and ER-β-/- mice, UV-induced immunosuppression was universally observed. In female Skh:hr1 and C57BL/6 mice, the immunosuppression was decreased by 1,25(OH)2D dose-dependently, but not in male Skh:hr1, ER-β-/- or ER-blockaded mice. These results reveal a sex bias in genetic, inflammatory and immune photoprotection by 1,25(OH)2D favoring female mice, that is dependent on the presence of ER-β.

scholarly journals Sex Differences in Photoprotective Responses to 1,25-Dihydroxyvitamin D3 in Mice Are Modulated by the Estrogen Receptor-β

2021 ◽  
Vol 22 (4) ◽  
pp. 1962
Author(s):  
Wannit Tongkao-on ◽  
Chen Yang ◽  
Bianca Y. McCarthy ◽  
Warusavithana G. Manori De Silva ◽  
Mark S. Rybchyn ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptor Beta ◽  
Contact Hypersensitivity ◽  
Cyclobutane Pyrimidine Dimers ◽  
Dihydroxyvitamin D3 ◽  
Female Mice ◽  
1,25 Dihydroxyvitamin D3 ◽  
Pyrimidine Dimers ◽  
Uv Induction ◽  
Receptor Beta

Susceptibility to photoimmune suppression and photocarcinogenesis is greater in male than in female humans and mice and is exacerbated in female estrogen receptor-beta knockout (ER-β−/−) mice. We previously reported that the active vitamin D hormone, 1,25-dihydroxyvitamin D3 (1,25(OH)2D), applied topically protects against the ultraviolet radiation (UV) induction of cutaneous cyclobutane pyrimidine dimers (CPDs) and the suppression of contact hypersensitivity (CHS) in female mice. Here, we compare these responses in female versus male Skh:hr1 mice, in ER-β−/−/−− versus wild-type C57BL/6 mice, and in female ER-blockaded Skh:hr1 mice. The induction of CPDs was significantly greater in male than female Skh:hr1 mice and was more effectively reduced by 1,25(OH)2D in female Skh:hr1 and C57BL/6 mice than in male Skh:hr1 or ER-β−/− mice, respectively. This correlated with the reduced sunburn inflammation due to 1,25(OH)2D in female but not male Skh:hr1 mice. Furthermore, although 1,25(OH)2D alone dose-dependently suppressed basal CHS responses in male Skh:hr1 and ER-β−/− mice, UV-induced immunosuppression was universally observed. In female Skh:hr1 and C57BL/6 mice, the immunosuppression was decreased by 1,25(OH)2D dose-dependently, but not in male Skh:hr1, ER-β−/−, or ER-blockaded mice. These results reveal a sex bias in genetic, inflammatory, and immune photoprotection by 1,25(OH)2D favoring female mice that is dependent on the presence of ER-β.

scholarly journals Estrogen receptor beta-deficient female mice develop a bladder phenotype resembling human interstitial cystitis

2007 ◽  
Vol 104 (23) ◽  
pp. 9806-9809 ◽  
Author(s):  
O. Imamov ◽  
K. Yakimchuk ◽  
A. Morani ◽  
T. Schwend ◽  
O. Wada-Hiraike ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Interstitial Cystitis ◽  
Estrogen Receptor Beta ◽  
Female Mice ◽  
Receptor Beta

scholarly journals Survival of reproductive behaviors in estrogen receptor beta gene-deficient (beta ERKO) male and female mice

1999 ◽  
Vol 96 (22) ◽  
pp. 12887-12892 ◽  
Author(s):  
S. Ogawa ◽  
J. Chan ◽  
A. E. Chester ◽  
J.-A. Gustafsson ◽  
K. S. Korach ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptor Beta ◽  
Reproductive Behaviors ◽  
Male And Female ◽  
Female Mice ◽  
Beta Gene ◽  
Receptor Beta

scholarly journals Deletion of Estrogen Receptor Beta in Osteoprogenitor Cells Increases Trabecular but Not Cortical Bone Mass in Female Mice

2015 ◽  
Vol 31 (3) ◽  
pp. 606-614 ◽  
Author(s):  
Kristy M Nicks ◽  
Koji Fujita ◽  
Daniel Fraser ◽  
Ulrike McGregor ◽  
Matthew T Drake ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Bone Mass ◽  
Cortical Bone ◽  
Estrogen Receptor Beta ◽  
Osteoprogenitor Cells ◽  
Female Mice ◽  
Cortical Bone Mass ◽  
Receptor Beta

scholarly journals Estrogen inhibits the vascular injury response in estrogen receptor beta -deficient female mice

1999 ◽  
Vol 96 (26) ◽  
pp. 15133-15136 ◽  
Author(s):  
R. H. Karas ◽  
J. B. Hodgin ◽  
M. Kwoun ◽  
J. H. Krege ◽  
M. Aronovitz ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Vascular Injury ◽  
Estrogen Receptor Beta ◽  
Injury Response ◽  
Female Mice ◽  
Receptor Beta

scholarly journals Natural Herbal Estrogen-Mimetics (Phytoestrogens) Promote the Differentiation of Fallopian Tube Epithelium into Multi-Ciliated Cells via Estrogen Receptor Beta

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 722
Author(s):  
Maobi Zhu ◽  
Sen Takeda ◽  
Tomohiko Iwano
Keyword(s):  
Estrogen Receptor ◽  
Cell Differentiation ◽  
Epithelial Cells ◽  
Fallopian Tube ◽  
Ciliated Cell ◽  
Estrogen Receptor Beta ◽  
Notch Pathway ◽  
Secretory Cells ◽  
Ciliated Cells ◽  
Receptor Beta

Phytoestrogens are herbal polyphenolic compounds that exert various estrogen-like effects in animals and can be taken in easily from a foodstuff in daily life. The fallopian tube lumen, where transportation of the oocyte occurs, is lined with secretory cells and multi-ciliated epithelial cells. Recently, we showed that estrogen induces multi-ciliogenesis in the porcine fallopian tube epithelial cells (FTECs) through the activation of the estrogen receptor beta (ERβ) pathway and simultaneous inhibition of the Notch pathway. Thus, ingested phytoestrogens may induce FTEC ciliogenesis and thereby affect the fecundity. To address this issue, we added isoflavones (genistein, daidzein, or glycitin) and coumestan (coumestrol) to primary culture FTECs under air–liquid interface conditions and assessed the effects of each compound. All phytoestrogens except glycitin induced multi-ciliated cell differentiation, which followed Notch signal downregulation. On the contrary, the differentiation of secretory cells decreased slightly. Furthermore, genistein and daidzein had a slight effect on the proportion of proliferating cells exhibited by Ki67 expression. Ciliated-cell differentiation is inhibited by the ERβ antagonist, PHTPP. Thus, this study suggests that phytoestrogens can improve the fallopian tube epithelial sheet homeostasis by facilitating the genesis of multi-ciliated cells and this effect depends on the ERβ-mediated pathway.

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