scholarly journals Deletion of Estrogen Receptor Beta in Osteoprogenitor Cells Increases Trabecular but Not Cortical Bone Mass in Female Mice

2015 ◽  
Vol 31 (3) ◽  
pp. 606-614 ◽  
Author(s):  
Kristy M Nicks ◽  
Koji Fujita ◽  
Daniel Fraser ◽  
Ulrike McGregor ◽  
Matthew T Drake ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Bone Mass ◽  
Cortical Bone ◽  
Estrogen Receptor Beta ◽  
Osteoprogenitor Cells ◽  
Female Mice ◽  
Cortical Bone Mass ◽  
Receptor Beta

scholarly journals Female Mice Lacking Estrogen Receptor-α in Hypothalamic Proopiomelanocortin (POMC) Neurons Display Enhanced Estrogenic Response on Cortical Bone Mass

Endocrinology ◽  
2016 ◽  
Vol 157 (8) ◽  
pp. 3242-3252 ◽  
Author(s):  
H. H. Farman ◽  
S. H. Windahl ◽  
L. Westberg ◽  
H. Isaksson ◽  
E. Egecioglu ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Bone Mass ◽  
Mechanical Strength ◽  
Cortical Bone ◽  
Volume Fraction ◽  
Bone Thickness ◽  
Female Mice ◽  
Cortical Bone Thickness ◽  
Cortical Bone Mass ◽  
Estrogenic Responses

Estrogens are important regulators of bone mass and their effects are mainly mediated via estrogen receptor (ER)α. Central ERα exerts an inhibitory role on bone mass. ERα is highly expressed in the arcuate (ARC) and the ventromedial (VMN) nuclei in the hypothalamus. To test whether ERα in proopiomelanocortin (POMC) neurons, located in ARC, is involved in the regulation of bone mass, we used mice lacking ERα expression specifically in POMC neurons (POMC-ERα−/−). Female POMC-ERα−/− and control mice were ovariectomized (OVX) and treated with vehicle or estradiol (0.5 μg/d) for 6 weeks. As expected, estradiol treatment increased the cortical bone thickness in femur, the cortical bone mechanical strength in tibia and the trabecular bone volume fraction in both femur and vertebrae in OVX control mice. Importantly, the estrogenic responses were substantially increased in OVX POMC-ERα−/− mice compared with the estrogenic responses in OVX control mice for cortical bone thickness (+126 ± 34%, P < .01) and mechanical strength (+193 ± 38%, P < .01). To test whether ERα in VMN is involved in the regulation of bone mass, ERα was silenced using an adeno-associated viral vector. Silencing of ERα in hypothalamic VMN resulted in unchanged bone mass. In conclusion, mice lacking ERα in POMC neurons display enhanced estrogenic response on cortical bone mass and mechanical strength. We propose that the balance between inhibitory effects of central ERα activity in hypothalamic POMC neurons in ARC and stimulatory peripheral ERα-mediated effects in bone determines cortical bone mass in female mice.

Mice lacking estrogen receptor [alpha] in hypothalamic POMC neurons display enhanced estrogenic response on cortical bone mass

2016 ◽  
Author(s):  
Helen Farman ◽  
Sara Windahl ◽  
Deborah Clegg ◽  
Shang Kui Xie ◽  
Lars Westberg ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Bone Mass ◽  
Cortical Bone ◽  
Estrogen Receptor Alpha ◽  
Receptor Alpha ◽  
Cortical Bone Mass

scholarly journals Estrogen receptor beta-deficient female mice develop a bladder phenotype resembling human interstitial cystitis

2007 ◽  
Vol 104 (23) ◽  
pp. 9806-9809 ◽  
Author(s):  
O. Imamov ◽  
K. Yakimchuk ◽  
A. Morani ◽  
T. Schwend ◽  
O. Wada-Hiraike ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Interstitial Cystitis ◽  
Estrogen Receptor Beta ◽  
Female Mice ◽  
Receptor Beta

scholarly journals Survival of reproductive behaviors in estrogen receptor beta gene-deficient (beta ERKO) male and female mice

1999 ◽  
Vol 96 (22) ◽  
pp. 12887-12892 ◽  
Author(s):  
S. Ogawa ◽  
J. Chan ◽  
A. E. Chester ◽  
J.-A. Gustafsson ◽  
K. S. Korach ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptor Beta ◽  
Reproductive Behaviors ◽  
Male And Female ◽  
Female Mice ◽  
Beta Gene ◽  
Receptor Beta

scholarly journals Sex Differences in Photoprotective Responses to 1,25-Dihydroxyvitamin D3 in Mice Are Modulated by the Estrogen Receptor-β

2021 ◽  
Vol 22 (4) ◽  
pp. 1962
Author(s):  
Wannit Tongkao-on ◽  
Chen Yang ◽  
Bianca Y. McCarthy ◽  
Warusavithana G. Manori De Silva ◽  
Mark S. Rybchyn ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptor Beta ◽  
Contact Hypersensitivity ◽  
Cyclobutane Pyrimidine Dimers ◽  
Dihydroxyvitamin D3 ◽  
Female Mice ◽  
1,25 Dihydroxyvitamin D3 ◽  
Pyrimidine Dimers ◽  
Uv Induction ◽  
Receptor Beta

Susceptibility to photoimmune suppression and photocarcinogenesis is greater in male than in female humans and mice and is exacerbated in female estrogen receptor-beta knockout (ER-β−/−) mice. We previously reported that the active vitamin D hormone, 1,25-dihydroxyvitamin D3 (1,25(OH)2D), applied topically protects against the ultraviolet radiation (UV) induction of cutaneous cyclobutane pyrimidine dimers (CPDs) and the suppression of contact hypersensitivity (CHS) in female mice. Here, we compare these responses in female versus male Skh:hr1 mice, in ER-β−/−/−− versus wild-type C57BL/6 mice, and in female ER-blockaded Skh:hr1 mice. The induction of CPDs was significantly greater in male than female Skh:hr1 mice and was more effectively reduced by 1,25(OH)2D in female Skh:hr1 and C57BL/6 mice than in male Skh:hr1 or ER-β−/− mice, respectively. This correlated with the reduced sunburn inflammation due to 1,25(OH)2D in female but not male Skh:hr1 mice. Furthermore, although 1,25(OH)2D alone dose-dependently suppressed basal CHS responses in male Skh:hr1 and ER-β−/− mice, UV-induced immunosuppression was universally observed. In female Skh:hr1 and C57BL/6 mice, the immunosuppression was decreased by 1,25(OH)2D dose-dependently, but not in male Skh:hr1, ER-β−/−, or ER-blockaded mice. These results reveal a sex bias in genetic, inflammatory, and immune photoprotection by 1,25(OH)2D favoring female mice that is dependent on the presence of ER-β.

scholarly journals Sex Differences in Photoprotective Responses to 1,25- Dihydroxyvitamin D3 in Mice are Modulated by the Estrogen Receptor-β

2020 ◽  
Author(s):  
Wannit Tongkao-on ◽  
Chen Yang ◽  
Bianca Y McCarthy ◽  
Warusavithana GM De Silva ◽  
Mark S Rybchyn ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptor Beta ◽  
Contact Hypersensitivity ◽  
Cyclobutane Pyrimidine Dimers ◽  
Dihydroxyvitamin D3 ◽  
Female Mice ◽  
1,25 Dihydroxyvitamin D3 ◽  
Pyrimidine Dimers ◽  
Uv Induction ◽  
Receptor Beta

Susceptibility to photoimmune suppression and photocarcinogenesis is greater in male than in female humans and mice and is exacerbated in female estrogen receptor-beta knockout (ER-β-/-) mice. We previously reported that the active vitamin D hormone, 1,25-dihydroxyvitamin D3 (1,25(OH)2D) applied topically protects against ultraviolet radiation (UV)-induction of cutaneous cyclobutane pyrimidine dimers (CPDs) and suppression of contact hypersensitivity (CHS) in female mice. Here we compare these responses in female versus male Skh:hr1 mice, in ER-β-/- versus wild type C57BL/6 mice, and in female ER-blockaded Skh:hr1 mice. Induction of CPDs was significantly greater in male than female Skh:hr1 mice and was more effectively reduced by 1,25(OH)2D in female Skh:hr1 and C57BL/6 mice, than in male Skh:hr1 or ER-β-/- mice respectively. This correlated with reduced sunburn inflammation by 1,25(OH)2D in female but not male Skh:hr1 mice. Furthermore, although 1,25(OH)2D alone dose-dependently suppressed basal CHS responses in male Skh:hr1 and ER-β-/- mice, UV-induced immunosuppression was universally observed. In female Skh:hr1 and C57BL/6 mice, the immunosuppression was decreased by 1,25(OH)2D dose-dependently, but not in male Skh:hr1, ER-β-/- or ER-blockaded mice. These results reveal a sex bias in genetic, inflammatory and immune photoprotection by 1,25(OH)2D favoring female mice, that is dependent on the presence of ER-β.

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