scholarly journals PROTEIN HYDROLYSATE OF POULTRY BY-PRODUCT AND SWINE LIVER IN THE DIET OF PACIFIC WHITE SHRIMP

2021 ◽  
Vol 47 ◽  
pp. e657
Author(s):  
Mariana SOARES ◽  
Priscila GONÇALVES ◽  
Delano Dias SCHLEDER ◽  
Mariana DELGADILLO-DÍAZ ◽  
Mariel GULLIAN-KLANIAN ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Intestinal Microbiota ◽  
Litopenaeus Vannamei ◽  
Amylase Activity ◽  
Protein Hydrolysate ◽  
Pacific White Shrimp ◽  
Metagenomic Analysis ◽  
White Shrimp ◽  
Control Group ◽  
Swine Liver

This study aimed to evaluate the use of protein hydrolysate of poultry by-product and swine liver in the diet of Litopenaeus vannamei and its effect on the intestinal microbiota and on the enzymatic activity of the hepatopancreas. Shrimp (10.94 ± 0.90 g) were fed with diets containing 0%, 25%, 50%, 75% and 100% of replacement of salmon by-product meal by protein hydrolysate, in triplicate. The hepatopancreas enzymatic activity and composition of intestinal microbiota was studied. It was observed that the protein hydrolysate in the diet changed the enzymatic activity of the shrimp when compared to the control group (p < 0.05). Amylase activity increases directly with the percent of protein replacement in the diet. Metagenomic analysis revealed change in the gut biome of the shrimps. The increasing levels of protein replacement provided greater richness in the 75% and 100% treatments, were mainly related to changes in the abundances in the families Rhodobacteraceae and Flavobacteriaceae. A reduction in the abundance of the Vibrionaceae family was observed with the inclusion of protein hydrolysate in the diet. These results indicate that the protein hydrolysate demonstrated beneficial changes when added at concentrations of 25% in the diet of L. vannamei.

Characterization of the intestinal microbiota in Pacific white shrimp, Litopenaeus vannamei, fed diets with different lipid sources

Aquaculture ◽  
2014 ◽  
Vol 434 ◽  
pp. 449-455 ◽  
Author(s):  
Meiling Zhang ◽  
Yuhong Sun ◽  
Ke Chen ◽  
Na Yu ◽  
Zhigang Zhou ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Litopenaeus Vannamei ◽  
Pacific White Shrimp ◽  
White Shrimp

Use of Cold Atmospheric Plasma To Preserve the Quality of White Shrimp (Litopenaeus vannamei)

2019 ◽  
Vol 82 (7) ◽  
pp. 1217-1223 ◽  
Author(s):  
DE ANGELIS de SOUZA SILVA ◽  
MARIA CARLA da SILVA CAMPELO ◽  
LUCAS de OLIVEIRA SOARES REBOUÇAS ◽  
JUSSIER de OLIVEIRA VITORIANO ◽  
CLODOMIRO ALVES ◽  
...  
Keyword(s):  
Shelf Life ◽  
Litopenaeus Vannamei ◽  
Cold Plasma ◽  
Bacterial Load ◽  
Pacific White Shrimp ◽  
Atmospheric Plasma ◽  
White Shrimp ◽  
Enzymatic Oxidation ◽  
Economic Losses ◽  
Control Group

ABSTRACT The enzymatic oxidation process that causes black spots on shrimp, named melanosis, leads to spoilage and economic losses. Therefore, there is an urgent need for strategies to reduce melanosis in the food industry. Cold plasma is an emerging nonthermal food processing technology. This work explores a novel application of cold plasma for the preservation of shrimp quality. This study aimed to evaluate the effects of cold plasma on the quality attributes of Pacific white shrimp (Litopenaeus vannamei). The shrimp samples were divided in two groups: the control group and the plasma group. Samples were exposed to nonthermal plasma for 10 min at a frequency of 500 Hz. Microbiological assays, including total counts of mesophilic and psychrotrophic bacteria, Staphylococcus spp., and Salmonella sp., were performed along with investigations of physicochemical parameters, such as pH, color, water-holding capacity, cooking loss, and shear force. In addition, sensory (quality index method) tests were conducted. The plasma group exhibited a shelf life of 14.07 days, while the control group exhibited a shelf life of 9.78 days. The experimental protocol used in this study was not enough to obtain significant reduction in the bacterial load. However, treatment with the cold plasma contributed to improving the physicochemical properties during storage. HIGHLIGHTS

scholarly journals Evaluation of the Dietary Black Soldier Fly Larvae Meal (Hermetia illucens) on Growth Performance, Intestinal Health, and Disease Resistance to Vibrio parahaemolyticus of the Pacific White Shrimp (Litopenaeus vannamei)

2021 ◽  
Vol 8 ◽  
Author(s):  
Yongkang Chen ◽  
Shuyan Chi ◽  
Shuang Zhang ◽  
Xiaohui Dong ◽  
Qihui Yang ◽  
...  
Keyword(s):  
Growth Performance ◽  
Intestinal Microbiota ◽  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Mrna Levels ◽  
Black Soldier Fly ◽  
The Pacific ◽  
Black Soldier Fly Larvae

The present study evaluated the effects of the dietary black soldier fly larvae meal (BSF) on growth performance, intestinal health, and susceptibility to Vibrio parahaemolyticus in the Pacific white shrimp Litopenaeus vannamei. The basal diet was formulated to contain 25% fish meal (FM), and then the FM was replaced with BSF for 10, 20, and 30% of the FM protein in the experimental diets, which are referred to as FM, BSF10, BSF20, and BSF30, respectively. Four hundred and eighty healthy and uniform-sized shrimp (~0.88 g) were distributed among four groups of three replicates, each with 40 shrimp in a 300-L tank and they were fed four times daily for 7 weeks. The results showed that the growth performance did not change significantly in shrimp fed with BSF10 and BSF20 diets, but significantly decreased in those fed with BSF30 diet compared to the ones fed with FM diet. After feeding trial and sampling, a V. parahaemolyticus infection challenge trial was conducted on shrimp. The results showed that the survival rate of shrimp fed with BSF10 was significantly higher than those fed with FM. The results of the midgut histology showed that the width and height of intestinal mucosal folds decreased significantly in shrimp fed with BSF20 and BSF30, and the early signs of apoptosis in the intestinal cells were found in shrimp fed with BSF30. The mRNA levels of non-specific immune-related genes dorsal and relish were downregulated in shrimp fed with BSF20 and BSF30 diets. The mRNA levels of antimicrobial peptides–related genes alf (anti-lipopolysaccharide factor) were upregulated in shrimp fed with BSF10 but downregulated in shrimp fed with BSF30. The mRNA level pen3 (penaeidins 3) was upregulated in shrimp fed with BSF10 and BSF20 diets. The intestinal bacterial communities on operational taxonomic unit levels among groups were not significantly differentiated according to the beta diversity analysis. At the genus levels, a decrease in Vibrio, Photobacterium, and Candidatus_Bacilloplasma, as well as the increase in Bacillus and Pseudoalteromonas abundance, indicated the improvement of intestinal microbiota in shrimp fed with dietary BSF. Therefore, the use of BSF in shrimp diet should be controlled at a dosage of 20% of the FM, which can improve the intestinal microbiota without causing any negative effects.

scholarly journals Molecular effects of mannanase-hydrolyzed coprameal to intestinal immunity in the Pacific white shrimp (Litopenaeus vannamei)

2021 ◽  
Author(s):  
Wanilada Rungrassamee ◽  
Sopacha Arayamethakorn ◽  
Nitsara Karoonuthaisiri ◽  
Shih-Chu Chen ◽  
Eric Chang ◽  
...  
Keyword(s):  
Immune System ◽  
Litopenaeus Vannamei ◽  
Control Diet ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Control Group ◽  
Intestinal Immunity ◽  
Toll Like Receptor ◽  
Transcript Levels ◽  
Significant Difference

AbstractTo mitigate disease outbreak, an alternative approach through enhancing shrimp immunity was explored. Mannan oligosaccharides (MOS) have been previously reported to enhance shrimp immune system. Here, coprameal samples were digested with mannanase to yield MOS, namely, mannanase-hydrolyzed coprameal (MCM) and feasibility of MCM as shrimp immunostimulant in grow-out ponds was determined. Pacific white shrimp (Litopenaeus vannamei) were fed with the commercial diet containing 1% MCM as the MCM-supplemented group and compared to the non-MCM control diet. There was no significant difference in survival rates between the MCM-supplemented and the control groups throughout the 4-month-period of the trial (p > 0.05). Gene expression analysis in shrimp intestines revealed that the transcript levels of antimicrobial peptides (anti-lipopolysaccharide factor isoform 1 (alf1), penaeidin (pen3a) and crustin (crus)) and lysozymes (lyz) were not significantly different in the MCM-supplemented group. Meanwhile, C-type lectin and toll-like receptor transcript levels, whose gene products play roles as pattern recognition proteins, were significantly higher in a group fed with MCM for 2- and 4-month periods than those of the control group (p < 0.05). The increased transcript levels of C-type lectin and toll-like receptor provide evidence for potential implementation of MCM as feed supplement to modulate shrimp immune system.

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