IDENTIFICATION AND ESTIMATION OF NON-PERMITTED FOOD COLOURS (METANIL YELLOW AND ANILINE DYES) IN TURMERIC POWDER BY RAPID COLOR TEST AND THIN LAYER CHROMATOGRAPHY

Author(s):  
Ritika Shah
Nature ◽  
1963 ◽  
Vol 199 (4891) ◽  
pp. 372-373 ◽  
Author(s):  
J. F. BARRETT ◽  
A. J. RYAN

Author(s):  
Siti Hamdanah ◽  
Syariful Anam ◽  
Jamaluddin Jamaluddin

Star fruits (Averrhoa bilimbi L.) from family of oxalidaceae is a plant which has potential as an antibacterial Staphylococcus aureus and Escherichia coli. Flavonoid are the active compounds from the extract of star fruit which has potential as an antibacterial. Star fruit were extracting by ethanol 96 % using maceration. Extract were identified using thin layer chromatography (TLC) with eluent analytic n-butanol- glacial acetic acid- water (6:1:3). Preparative isolation using thin layer chromatography resulting three bands. Each band was centrifuged to obtain isolates. Physics isolates were analyzing using spectrophotometer UV Vis and chemically using color test (shift reagent). UV Vis spectrum results with shift reagent from isolates 1, suspected as isoflavone compound with OH groups in position 6,7,4 and OH groups in O- ring A, in isolates 2 was not flavonoid compound and predicted as other polyphenolic compound and in isolates 3 suspected of flavone compound with OH groups in position 5,6,7 and OH groups in O-ring A


2005 ◽  
Vol 3 (1) ◽  
pp. 32-38
Author(s):  
KHOIRINA DWI NUGRAHANINGTYAS ◽  
SABIRIN MATSJEH ◽  
TUTIK DWI WAHYUNI

This research was aimed to isolate and identify the flavonoid compounds from curcuma’ rhizome (Curcuma aeruginosa Roxb: Zingiberaceae). The extraction was carried out by Soxhlet method using petroleum eter, chloroform, nbutanol and methanol as the solvent agent. Those extract then qualitatively tested to identify the presence of flavonoid. Flavonoid then isolated from the extract of petroleum eter by column chromatography. The fraction resulted from chromatographic was analyzed by thin layer chromatography (TLC) method. Flavonoid identification was tested by color test, spectrophotometer UV-Vis, IR and GC-MS. The color test result showed that extract of petroleum eter, chloroform and n-butanol contain flavonoid. The analyzed single fraction from column chromatography showed that f2 contains isoflavone with 2 methoxy and 1 ethyl substitutes, f4 contains isoflavone with 2 methoxy substitutes, then f9 contains isoflavone with 1 hydroxy and 2 methoxy substitutes.


Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.


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