Genome-wide identification and expression profile of YABBY genes in Averrhoa carambola

Background Members of the plant-specific YABBY gene family are thought to play an important role in the development of leaf, flower, and fruit. The YABBY genes have been characterized and regarded as vital contributors to fruit development in Arabidopsis thaliana and tomato, in contrast to that in the important tropical economic fruit star fruit (Averrhoa carambola), even though its genome is available. Methods In the present study, a total of eight YABBY family genes (named from AcYABBY1 to AcYABBY8) were identified from the genome of star fruit, and their phylogenetic relationships, functional domains and motif compositions, physicochemical properties, chromosome locations, gene structures, protomer elements, collinear analysis, selective pressure, and expression profiles were further analyzed. Results Eight AcYABBY genes (AcYABBYs) were clustered into five clades and were distributed on five chromosomes, and all of them had undergone negative selection. Tandem and fragment duplications rather than WGD contributed to YABBY gene number in the star fruit. Expression profiles of AcYABBYs from different organs and developmental stages of fleshy fruit indicated that AcYABBY4 may play a specific role in regulating fruit size. These results emphasize the need for further studies on the functions of AcYABBYs in fruit development.

Nefrotoxicidade e Neurotoxicidade Associadas ao Consumo da Averrhoa bilimbi e Averrhoa carambola: Uma Revisão Integrativa / Nephrotoxicity and Neurotoxicity associated with the Consumption of Averrhoa bilimbi and Averrhoa carambola: An Integrative Review

Resumo: As espécies Averrhoa bilimbi (birí-birí) e Averrhoa carambola (carambola) pertencem à família das Oxalidaceae, o fruto que é cultivado em vários países da América do Sul. Estas frutas são habitualmente consumidas pela população, seja na forma de sucos, geléias, doces e conservas. O grande problema é que seus consumos exagerados podem causar efeitos tóxicos que são desconhecidos pela população em geral, necessitando assim de maiores conhecimentos sobre a toxicidade destas plantas. Assim a presente pesquisa trata-se de uma revisão integrativa de literatura onde; após analisar os descritores em sites de busca na internet e aplicar os critérios de inclusão e exclusão pré-estabelecidos, foram selecionados um total de 14 artigos. Todos os artigos selecionados contemplavam de informações sobre a nefrotoxicidade dos frutos desencadeadas pela presença de sais de oxalato; bem como descreveram a neurotoxicidade na carambola desencadeada pela presença da caramboxina. Assim, observa-se a necessidade do conhecimento sobre os respectivos efeitos tóxicos dessas frutas e do conhecimento etnobotânico atribuído ao profissional farmacêutico que propaga informações sobre o uso racional da fitoterapia.Palavras-chave: Averrhoa bilimbi. Averrhoa carambola. Nefrotoxicidade. Neurotoxicidade. Abstract: The species Averrhoa bilimbi (biri biri) and Averrhoa carambola (star fruit) belong to the Oxalidaceae Family, the fruit is cultivated in several South American countries. These fruits are usually consumed by the population, whether in the form of juices, jellies, candy or preserve. The big problem is that the excessive consumption can cause toxic effects that are unknown by the general population, being necessary better knowledge about the toxicity of these plants. Thus, this research is about the integrative literature revision where; after analyzing the descriptors on websites and apply the pre-established inclusion and exclusion criteria, a total of 14 articles were selected. All selected articles contemplated the information about the nephrotoxicity of the fruits trigged by the presence of oxalate salt; as well as described the neurotoxicity of the star fruit trigged by the presence of caramboxin. In that way, there is a need for knowledge about these respective toxic effects of these fruits and for ethnobotanical knowledge assigned to the pharmacist that spreads information about the rational use of herbal medicine. Keywords: Averrhoa bilimbi. Averrhoa carambola. Nephrotoxicity. Neurotoxicity.

Small and medium enterprises' innovation and product competitiveness in the Covid-19 pandemic era

The purpose of the community service is to increase the products competitiveness of the Belimbing Wangi SMEs, Watesari Village, Balongbendo District, Sidoarjo Regency. The impact of the Covid-19 pandemic is extraordinary, especially for the Belimbing Wangi SMEs business group, where the production process and turnover has decreased, because business management is still carried out conventionally and Watesari Village tourism must be closed according to government regulations. The service method used is field observation, trial of processed star fruit products, training and assistance in processing star fruit juice, and business management training. As a result of the assistance, partners understand the importance of conducting business management and finding the best formula for star fruit juice drinks. This training also supports the sustainability of the Belimbing Wangi SMEs business and competes at the global market level.

Potential antioxidant effect of fruit peels for human use from northern Peru, compared by 5 different methods

The objective of the work was to determine the antioxidant potential in vitro of freeze-dried peel extracts of 20 fruits from the northern region of Peru through five tests (Folin-Ciocalteu, DPPH., ABTS+., FRAP and CUPRAC). According to multivariate statistical analyzes, five groups were found: (i.) peel extracts with the highest values of antioxidant capacity (AC) from custard apple, and star fruit; (ii.) rind extracts with high AC values from quince, sweet granadilla, guava, and black grape; (iii.) husk extracts with middle values of AC from passion fruit, and red mombin; (iv.) shell extracts with low AC values from tangerine, mandarine, and bitter orange; and, (v.) coating extracts with the lowest AC values from pawpaw, red pawpaw, muskmelon, dragon fruit, yellow and red indian figs, pear, apple, and green grape. To conclude, the fruit lyophilized-husk extracts of custard apple, star fruit, quince, sweet granadilla, guava, and black grape obtained the best AC.

Partisipasi Masyarakat Dalam Pengembangan Desa Wisata (Studi Kasus Desa Menang Kecamatan Pagu Kabupaten Kediri)

The village won has tourism potential, namely Petilasan Sri Aji Jaya Baya and star fruit. The purpose of this study was to determine community participation in the development of a tourist village in Menang Village which eventually became one of the sources of economic improvement for the surrounding community. The method used in this research is qualitative because it can provide an overview of community participation in the development of a tourist village. The analysis uses Max Weber's theory of rationality. Max Weber's rationality theory can explain community participation in developing tourist villages. Collecting information by means of interviews, observation, and documentation. Based on the research results, there is community participation in the form of ideas, energy, and creativity. This is done by the community to work together to develop the star fruit tourism village in an effort to improve the community's economy.

Manufacture of Toman Fish Protein (Channa micropeltes) Isolate with Different pH Methods using Star Fruit (Averrhoa bilimbi) Acid

Toman fish (Channa micropeltes) is a type of freshwater fish that belongs to the genus Channa which is the largest size compared to the others and has high protein. This study aims to obtain the isolate of toman fish protein (Channa micropeltes) by the method of pH regulation using star fruit acid. Wuluh star fruit acid is used as a substitute for HCl in making fish protein isolates to reduce pH. This research method uses a Completely Randomized Design (CRD). The treatments consisted of (P1 = pH 4, P2 = pH 5, P3 = pH 6). The results showed that star fruit acid can replace HCl as a protein precipitant and P1 (pH 4) obtained the most isolates, namely an average protein content of 91.64% (db), a moisture content of 1.67%, ash content of 2.06 % (db), fat content of 2.74% (db), yield of 88.46%, and total amino acid composition of 61.30%, where the type of amino acid that dominates is glutamic acid (12.30%). Glutamic acid amino acid is the one that influences a lot in giving pH isoelectric point, in the treatment of P1 with pH 4 making isolates of toman fish protein to be the best treatment, because glutamic acid has an isoelectric point of 3.22 which is the closest to pH 4 plus also the pH of isoelectric point from other amino acids.

FORMULASI DAN EVALUASI HAND SANITIZER SPRAY EKSTRAK BUAH BELIMBING WULUH (Averrhoa bilimbi L)

The tendency of society at this time to return to nature (Back to nature) is by using plants as treatment. The use of star fruit as the main ingredient in the manufacture of a hand sanitizer spray on the basis of star fruit has flavonoid compounds that have the potential as antibacterial. Hand sanitizer spray is made into 3 formulas with a concentration ratio of F1 (10%), F2 (12%), and F3 (15%). Each formula was subjected to physical tests in the form of organoleptic tests, pH tests, homogeneity tests and freeze and thaw stability tests for 6 storage cycles at 4°C and 40°C. The organoleptic results showed that the preparation did not change during four weeks of storage. On examination, the pH of the preparation decreased for F1 with a pH of 5.25 to 4.88; F2 with a pH of 5.14 becomes 4.77, and F3 with a pH of 4.87 becomes 4.60 but this value is still included in the specified range. The homogeneity of the preparations F1, F2, and F3 was only homogeneous in the first week and for the stability of the preparations F1, F2 and F3 were only stable in cycle 1 and cycle 2.

PHYTOCHEMICAL STUDIES OF STAR FRUIT AVERRHOA CARAMBOLA L.

The paper deals with a phytochemical investigation on the fruit of Averrhoa carambola L. belonging to the family Oxalidaceae. Commonly known as “star fruit”. Fruits and leaves are used widely in Ayurveda preparations to pacify impaired Kapha, pitta, skin diseases, pruritis, worm infestations, diarrhea, vomiting, hemorrhoids, intermittent fever, over-perspiration, and general debility. In the present study fruits of Averrhoa carambola L. were screened for their phytochemical constituents following hot continuous and successive extraction by Soxhlet apparatus. A qualitative assay was done using a range of solvents. The extraction process was carried using different solvents successively in the order of increasing polarity. Qualitative analysis of the extracts using standard procedures revealed the presence of alkaloids, avonoids, tannins, saponins, glycosides, steroids&terpenoids, phenols, coumarins, and phytosterols. Preliminary screening of phytochemicals is a valuable step, in the detection of the bioactive principles present in medicinal plants and subsequently, may lead to drug discovery and development.

AKTIVITAS ANTIDIABETES EKSTRAK ETANOL DAUN BELIMBING BESI (Averhhoa carrambola) PADA MENCIT (Mus musculus)

Many antioxidant compounds, flavonoids, and saponins are contained in starfruit leaves (Averrhoa carambola). The purpose of this study was to determine the antidiabetic activity of star fruit extract in mice. This study used a completely randomized design (CRD) with 6 treatments, namely P1 (aquadest without alloxan) as a negative control, P2 (aquadest with alloxan) as a positive control, P3 (metformin 500 mg / kgBB), P4 (alloxan + ethanol extract of star fruit leaves. 100 mg / kgBB), P5 (alloxan + ethanol extract of star fruit leaves 150 mg / kgBB), P6 (alloxan + ethanol extract of star fruit leaves 200 mg / kgBB). The starfruit leaf extract was made using the maceration method. In order for experimental animals to become diabetic, alloxan is used at a dose of 120 mg / kgBW which is injected intramuscularly into the thigh muscles. After the experimental animals were diabetic, We were given ethanol extract of starfruit leaves according to the experimental design, starting from day 7th to day 21st. Blood glucose levels are measured with a glucometer every week. The results showed that the best decrease in blood glucose levels occurred in the first week and the second week. In the first week, the metformin treatment was able to reduce blood glucose by 88.4%, while the ethanol extract treatment of star fruit leaves at a dose of 200 mg / kg BW was able to reduce blood glucose levels by 60%. The ethanol extract of star fruit leaves has antidiabetic activity. Keywords: mice, diabetes mellitus, blood glucose, starfruit leaves.