Cytokeratin 13, Cytokeratin 17, and Ki-67 Expression in Human Acquired Cholesteatoma and Their Correlation With Its Destructive Capacity

Abstract Background Oral lichen planus is a chronic inflammatory and immune-mediated disease that affects the oral mucosa. Recent findings have suggested that oral lichen planus is often associated with submucosal fibrotic changes. Fibrotic changes in the buccal submucosa may cause restricted mouth opening. This report discusses the histopathological examination (including specialized staining) and surgical treatment for oral lichen planus-induced fibrotic changes. Case presentation Here, we describe a 63-year-old woman who had oral lichen planus with fibrotic changes. Her maximum mouth opening distance was approximately 30 mm due to submucosal fibrotic changes, and she exhibited gradual fibrosis progression. Histological examinations were performed to assess the oral lichen planus-induced fibrotic changes. Then, double Z-plasty were performed as treatment for restricted mouth opening. The immunohistochemical staining results were negative for cytokeratin 13 and positive in some layers for cytokeratin 17 and Ki-67/MIB-1. Masson's trichrome staining showed enhanced collagen formation. Postoperative mouth opening training enabled the patient to achieve a mouth opening distance of > 50 mm. Conclusion Our findings suggest that histopathological examination with specialized staining can aid in the evaluation of oral lichen planus-induced fibrotic changes, and that Z-plasty is effective for the treatment of restricted mouth opening due to oral lichen planus.

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Histopathological evaluation including cytokeratin 13 and Ki-67 in the border between Lugol-stained and -unstained areas

Oncology Reports ◽

10.3892/or_00000822 ◽

2010 ◽

Vol 24 (1) ◽

Cited By ~ 18

Author(s):

Ohta

Keyword(s):

Ki 67 ◽

Histopathological Evaluation ◽

Cytokeratin 13

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Cytokeratin 17 and Ki-67: Immunohistochemical markers for the differential diagnosis of keratoacanthoma and squamous cell carcinoma

Oncology Letters ◽

10.3892/ol.2017.5793 ◽

2017 ◽

Vol 13 (4) ◽

pp. 2539-2548

Author(s):

Cem Leblebici ◽

Esra Pasaoglu ◽

Canan Kelten ◽

Seher Darakci ◽

Nevra Dursun

Keyword(s):

Squamous Cell Carcinoma ◽

Differential Diagnosis ◽

Cell Carcinoma ◽

Squamous Cell ◽

Ki 67 ◽

Cytokeratin 17 ◽

Immunohistochemical Markers

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Role of Langerhans cells, Ki-67 protein and apoptosis in acquired cholesteatoma: prospective clinical study

The Journal of Laryngology & Otology ◽

10.1017/s0022215112003180 ◽

2013 ◽

Vol 127 (3) ◽

pp. 252-259 ◽

Cited By ~ 3

Author(s):

V Akdogan ◽

I Yilmaz ◽

T Canpolat ◽

L N Ozluoglu

Keyword(s):

Middle Ear ◽

Langerhans Cells ◽

Prospective Clinical Study ◽

Ear Canal ◽

Ki 67 ◽

Middle Ear Cholesteatoma ◽

Proliferation And Apoptosis ◽

Acquired Cholesteatoma ◽

Open Versus

AbstractObjective:To investigate the role of Langerhans cells in the pathogenesis and clinical picture of middle-ear cholesteatoma.Subjects and methods:The study included 40 patients operated upon for a diagnosis of chronic otitis due to acquired cholesteatoma.Results and analysis:A closed surgical technique was used in 20 per cent of patients and an open technique in 80 per cent. Langerhans cells were more densely accumulated in cholesteatoma epithelium, compared with external ear canal skin (p < 0.001). Staining for Ki-67 protein was greater in cholesteatoma epithelium (p < 0.001) and Apo2.7 protein staining (indicating apoptosis) was more prominent (p < 0.001), compared with ear canal skin. Regarding significant relationships between clinical and pathological findings, staining for Ki-67 (p = 0.046) and Apo2.7 (p = 0.037) was more prominent in patients undergoing open versus closed surgery.Conclusion:Using cell proliferation and apoptosis markers, a dense Langerhans cell infiltration was found to occur as a host response to middle-ear cholesteatoma.

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Evaluation of Superficial Oral Squamous Cell Malignancy Based on Morphometry and Immunoexpression of Cytokeratin 13 and Cytokeratin 17

Acta Cytologica ◽

10.1159/000355865 ◽

2014 ◽

Vol 58 (1) ◽

pp. 67-75 ◽

Cited By ~ 5

Author(s):

Mitsumasa Yamashina ◽

Kazumichi Sato ◽

Morio Tonogi ◽

Yoichi Tanaka ◽

Gen-yuki Yamane ◽

...

Keyword(s):

Squamous Cell ◽

Cytokeratin 17 ◽

Cytokeratin 13 ◽

Cell Malignancy

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The significance of the expression of cell proliferation and inflammation markers in the development of acquired middle ear cholesteatoma

Vojnosanitetski pregled ◽

10.2298/vsp160830303e ◽

2018 ◽

Vol 75 (5) ◽

pp. 487-495

Author(s):

Milan Erdoglija ◽

Ugljesa Grgurevic ◽

Snezana Cerovic ◽

Milena Jovic ◽

Nenad Baletic ◽

...

Keyword(s):

Middle Ear ◽

External Auditory Canal ◽

Clinical Picture ◽

Clinical Characteristics ◽

Biological Behavior ◽

Ki 67 ◽

Middle Ear Cholesteatoma ◽

Significant Difference ◽

Acquired Cholesteatoma ◽

Cox 2

Background/Aim. Permanent proliferation and periodical infection are the main clinical characteristics of acquired middle ear cholesteatoma. The aim of this study was to research immunohistochemical characteristics of the skin along with the cholesteatoma process in the nearby tissue. This research should influence further studying of etiology and development of acquired middle ear cholesteatoma. Methods. We investigated clinical, histological and immunohistochemical characteristics of cholesteatoma in 50 samples from operated patients with acquired middle ear cholesteatomas. We classified all samples according to their clinical characteristics of cholesteatoma such as bone destruction, presence of infection or cholesteatoma extension and histological characteristics of cholesteatoma such as keratinisation, inflammatory process and extracellular matrix proliferation. We used mouse monoclonal antibodies for proliferating cell nuclear antigen (MAbs for PCNA), Ki-67, COX-2, CD 4 and CD 8 lymphocytes to investigate the expression of those characteristics in the cholesteatoma and in the control skin tissue. Statistical analyses were performed using SPSS for Windows version 16.0 (SPSS, Chicago, IL, USA). We used the independent group t-test, Spearman?s correlation analysis and Mann-Whitney U test to analyze statistical analysis. Results. Expression of PCNA, Ki-67, COX-2 and CD 8 lymphocytes in more serious clinical picture of cholesteatoma was almost equal as in less serious clinical picture of cholesteatoma. There was statistically significantly higher concentration of inflammation marker CD 4 lymphocytes, both in the acquired cholesteatoma and in the skin of bony portion of the external auditory canal near fibrocartilaginous annulus in more serious clinical picture of cholesteatoma than in less serious clinical picture of cholesteatoma (p < 0.01). There was statistically significant difference of expression of PCNA, Ki- 67, COX-2, CD 4 and CD 8 lymphocytes between all cholesteatoma samples and the skin of bony portion of the external auditory canal (p < 0.05) and statistically significant difference of expression of those markers between the skin of bony portion of the external auditory canal and retroauricular skin (p < 0.05). Conclusion. Inflammation of the skin of bony portion of the external auditory canal is a milestone in pathogenesis of acquired middle ear cholesteatoma. Expression of CD 4 lymphocytes can be the prognostic factor for acquired cholesteatoma clinical picture development. We found so much diversity in biological behavior through very different levels of cholesteatoma development. Expression of Ki-67 in acquired middle ear cholesteatoma is a reliable and stable marker of proliferation for acquired middle ear cholesteatoma.

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Differential Expression of Ki-67 and P27 in Cholesteatoma Compared to Skin Tissue Predicts the Prognosis of Adult Acquired Cholesteatoma

The Journal of International Advanced Otology ◽

10.5152/iao.2021.9453 ◽

2021 ◽

Vol 17 (4) ◽

pp. 306-312

Author(s):

Serkan Turkili ◽

◽

Kemal Gorur ◽

Onur Ismi ◽

Ebru Serinsoz Linke ◽

...

Keyword(s):

Differential Expression ◽

Skin Tissue ◽

Ki 67 ◽

Acquired Cholesteatoma

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Diagnostic utility of Cytokeratin 13 and Cytokeratin 17 in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma

Asian Pacific Journal of Cancer Biology ◽

10.31557/apjcb.2020.5.4.153-158 ◽

2020 ◽

Vol 5 (4) ◽

pp. 153-158

Author(s):

Maryam Nazir Kiani ◽

Muhammad Asif ◽

Fakeha Meraj Ansari ◽

Nighat Ara ◽

Muhammad Ishaque ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Epithelial Dysplasia ◽

P Value ◽

Oral Epithelial Dysplasia ◽

Cytokeratin 17 ◽

Cytokeratin 13 ◽

Oral Epithelial

Objective: To determine the immunohistochemical expression of CK 13 and CK 17 in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma. Methods: A total of 170 cases were retrieved from record files of Histopathology Department, to conduct a cross sectional study at Armed Forces Institute of Pathology, Rawalpindi, over a period of one year from June 2018 to June 2019 along with their formalin-fixed, paraffin embedded blocks comprising 85 cases of each oral squamous cell carcinoma and oral epithelial dysplasia. Blocks were trimmed and cut into very thin sections of 5 microns using microtome and mounted on slides. Tissue mounted slides were stained with routine haematoxylin and eosin followed by immunohistochemical staining of cytokeratin 13 and cytokeratin 17. New histological diagnosis of each case was made. Mean and standard deviation were calculated for quantitative variables. Frequency and percentages were calculated for qualitative variables. Chi-square test was employed to assess the significance of difference. The P-value <0.05 was considered significant. Results: In this study, 101 (59.4%) male and 69 (40.6%) female patients with the mean age of 60.63 ± 13.814 (mean ± SD) were collected and buccal mucosa was the most common site of presentation. In a total of 170 cases, 34 (20%) cases showed positive expression of cytokeratin 13 whereas 136 (80%) cases showed negative expression of cytokeratin 13. In comparison, out of 170 cases, 133 (78.2%) cases showed positive expression of cytokeratin 17 whereas 37 (21.8%) cases showed negative expression of cytokeratin 17. The P-value was found to be < 0.001 and 0.001 for expression of cytokeratin 13 and cytokeratin 17 respectively. Conclusion: Opposite expression of cytokeratin 13 and cytokeratin 17 was seen in this study, in the form of loss of cytokeratin 13 and over expression of cytokeratin 17 with increase in the degree of dysplasia and invasive carcinoma. Correct assessment, diagnosis and management with the help of these markers can lead to early diagnosis and favorable treatment outcome.

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