scholarly journals Differential Expression of Ki-67 and P27 in Cholesteatoma Compared to Skin Tissue Predicts the Prognosis of Adult Acquired Cholesteatoma

2021 ◽  
Vol 17 (4) ◽  
pp. 306-312
Author(s):  
Serkan Turkili ◽  
◽  
Kemal Gorur ◽  
Onur Ismi ◽  
Ebru Serinsoz Linke ◽  
...  
Keyword(s):  
Differential Expression ◽  
Skin Tissue ◽  
Ki 67 ◽  
Acquired Cholesteatoma

Differential Expression of p53 and Ki-67 Proteins in Classic and Iatrogenic Kaposi's Sarcoma

1999 ◽  
Vol 21 (2) ◽  
pp. 138-145 ◽  
Author(s):  
Emmilia Hodak ◽  
Ilan Hammel ◽  
Meora Feinmesser ◽  
Almog Zelinger ◽  
Lea Maron ◽  
...  
Keyword(s):  
Differential Expression ◽  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Ki 67

scholarly journals Differential expression of cyclin D1, Ki‑67, pRb, and p53 in psoriatic skin lesions and normal skin

2017 ◽  
Author(s):  
Sung Kim ◽  
Young Ryu ◽  
Jun Kwon ◽  
Mi Choe ◽  
Jin Jung ◽  
...  
Keyword(s):  
Cyclin D1 ◽  
Differential Expression ◽  
Normal Skin ◽  
Skin Lesions ◽  
Psoriatic Skin ◽  
Ki 67

Differential expression of DUSP6 with expression of ERK and Ki-67 in non-small cell lung carcinoma

2011 ◽  
Vol 207 (7) ◽  
pp. 428-432 ◽  
Author(s):  
Hyunjung Lee ◽  
Jin Man Kim ◽  
Song-Mei Huang ◽  
Seung-Kiel Park ◽  
Dong-Hoon Kim ◽  
...  
Keyword(s):  
Differential Expression ◽  
Lung Carcinoma ◽  
Small Cell Lung Carcinoma ◽  
Small Cell ◽  
Small Cell Lung ◽  
Ki 67 ◽  
Cell Lung Carcinoma

Role of Langerhans cells, Ki-67 protein and apoptosis in acquired cholesteatoma: prospective clinical study

2013 ◽  
Vol 127 (3) ◽  
pp. 252-259 ◽  
Author(s):  
V Akdogan ◽  
I Yilmaz ◽  
T Canpolat ◽  
L N Ozluoglu
Keyword(s):  
Middle Ear ◽  
Langerhans Cells ◽  
Prospective Clinical Study ◽  
Ear Canal ◽  
Ki 67 ◽  
Middle Ear Cholesteatoma ◽  
Proliferation And Apoptosis ◽  
Acquired Cholesteatoma ◽  
Open Versus

AbstractObjective:To investigate the role of Langerhans cells in the pathogenesis and clinical picture of middle-ear cholesteatoma.Subjects and methods:The study included 40 patients operated upon for a diagnosis of chronic otitis due to acquired cholesteatoma.Results and analysis:A closed surgical technique was used in 20 per cent of patients and an open technique in 80 per cent. Langerhans cells were more densely accumulated in cholesteatoma epithelium, compared with external ear canal skin (p < 0.001). Staining for Ki-67 protein was greater in cholesteatoma epithelium (p < 0.001) and Apo2.7 protein staining (indicating apoptosis) was more prominent (p < 0.001), compared with ear canal skin. Regarding significant relationships between clinical and pathological findings, staining for Ki-67 (p = 0.046) and Apo2.7 (p = 0.037) was more prominent in patients undergoing open versus closed surgery.Conclusion:Using cell proliferation and apoptosis markers, a dense Langerhans cell infiltration was found to occur as a host response to middle-ear cholesteatoma.

scholarly journals Cytokeratin 13, Cytokeratin 17, and Ki-67 Expression in Human Acquired Cholesteatoma and Their Correlation With Its Destructive Capacity

2017 ◽  
Vol 10 (3) ◽  
pp. 213-220 ◽  
Author(s):  
Mahmood A. Hamed ◽  
Seiichi Nakata ◽  
Kazuya Shiogama ◽  
Kenji Suzuki ◽  
Ramadan H. Sayed ◽  
...  
Keyword(s):  
Ki 67 ◽  
Cytokeratin 17 ◽  
Acquired Cholesteatoma ◽  
Cytokeratin 13

Differential expression of the suppressor PML and Ki-67 identifies three subtypes of human nasopharyngeal carcinoma

2002 ◽  
Vol 38 (12) ◽  
pp. 1600-1606 ◽  
Author(s):  
J.Y.H Chan ◽  
C.L Meng ◽  
K.F To ◽  
S.F Leung ◽  
A.T.C Chan ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Differential Expression ◽  
Ki 67 ◽  
Human Nasopharyngeal Carcinoma

scholarly journals Differential expression of neuroendocrine markers, TTF-1, p53, and Ki-67 in cervical and pulmonary small cell carcinoma

Medicine ◽  
2018 ◽  
Vol 97 (30) ◽  
pp. e11604 ◽  
Author(s):  
Haiping Liu ◽  
Yan Zhang ◽  
Jianfang Chang ◽  
Zhitao Liu ◽  
Ning Tang
Keyword(s):  
Cell Carcinoma ◽  
Differential Expression ◽  
Small Cell Carcinoma ◽  
Small Cell ◽  
Ki 67 ◽  
Neuroendocrine Markers

scholarly journals The significance of the expression of cell proliferation and inflammation markers in the development of acquired middle ear cholesteatoma

2018 ◽  
Vol 75 (5) ◽  
pp. 487-495
Author(s):  
Milan Erdoglija ◽  
Ugljesa Grgurevic ◽  
Snezana Cerovic ◽  
Milena Jovic ◽  
Nenad Baletic ◽  
...  
Keyword(s):  
Middle Ear ◽  
External Auditory Canal ◽  
Clinical Picture ◽  
Clinical Characteristics ◽  
Biological Behavior ◽  
Ki 67 ◽  
Middle Ear Cholesteatoma ◽  
Significant Difference ◽  
Acquired Cholesteatoma ◽  
Cox 2

Background/Aim. Permanent proliferation and periodical infection are the main clinical characteristics of acquired middle ear cholesteatoma. The aim of this study was to research immunohistochemical characteristics of the skin along with the cholesteatoma process in the nearby tissue. This research should influence further studying of etiology and development of acquired middle ear cholesteatoma. Methods. We investigated clinical, histological and immunohistochemical characteristics of cholesteatoma in 50 samples from operated patients with acquired middle ear cholesteatomas. We classified all samples according to their clinical characteristics of cholesteatoma such as bone destruction, presence of infection or cholesteatoma extension and histological characteristics of cholesteatoma such as keratinisation, inflammatory process and extracellular matrix proliferation. We used mouse monoclonal antibodies for proliferating cell nuclear antigen (MAbs for PCNA), Ki-67, COX-2, CD 4 and CD 8 lymphocytes to investigate the expression of those characteristics in the cholesteatoma and in the control skin tissue. Statistical analyses were performed using SPSS for Windows version 16.0 (SPSS, Chicago, IL, USA). We used the independent group t-test, Spearman?s correlation analysis and Mann-Whitney U test to analyze statistical analysis. Results. Expression of PCNA, Ki-67, COX-2 and CD 8 lymphocytes in more serious clinical picture of cholesteatoma was almost equal as in less serious clinical picture of cholesteatoma. There was statistically significantly higher concentration of inflammation marker CD 4 lymphocytes, both in the acquired cholesteatoma and in the skin of bony portion of the external auditory canal near fibrocartilaginous annulus in more serious clinical picture of cholesteatoma than in less serious clinical picture of cholesteatoma (p < 0.01). There was statistically significant difference of expression of PCNA, Ki- 67, COX-2, CD 4 and CD 8 lymphocytes between all cholesteatoma samples and the skin of bony portion of the external auditory canal (p < 0.05) and statistically significant difference of expression of those markers between the skin of bony portion of the external auditory canal and retroauricular skin (p < 0.05). Conclusion. Inflammation of the skin of bony portion of the external auditory canal is a milestone in pathogenesis of acquired middle ear cholesteatoma. Expression of CD 4 lymphocytes can be the prognostic factor for acquired cholesteatoma clinical picture development. We found so much diversity in biological behavior through very different levels of cholesteatoma development. Expression of Ki-67 in acquired middle ear cholesteatoma is a reliable and stable marker of proliferation for acquired middle ear cholesteatoma.

The Co-Culture Of Primary CLL Cells With Bone Marrow Mesenchymal Cells, CD40 Ligand and CpG ODN Promotes The Proliferation Of Chemoresistant CLL Cells Phenotypically Comparable To Those From The Proliferative Centers

Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 4153-4153
Author(s):  
Noelia Purroy ◽  
Pau Abrisqueta ◽  
Eva Calpe ◽  
Cecilia Carpio ◽  
Carles Palacio ◽  
...  
Keyword(s):  
Bone Marrow ◽  
B Cells ◽  
Differential Expression ◽  
Chemokine Receptors ◽  
Conflicts Of Interest ◽  
Ex Vivo ◽  
Lethal Dose ◽  
Cpg Odn ◽  
Ki 67 ◽  
Expression Levels

Abstract Chronic lymphocytic leukemia (CLL) is characterized by the accumulation and proliferation of monoclonal CD5+ mature B-cells in peripheral blood (PB), lymph nodes (LN), and bone marrow (BM). The microenvironment found in BM and LN, where CLL cells interact with different accessory cells, induces active proliferation of CLL cells and protects them from spontaneous and chemotherapy-induced apoptosis. This may explain why the BM and secondary lymphoid organs are preferential sites for minimal residual disease persistence and for relapses observed in all patients after treatment. Therefore, further characterization of primary CLL cells found in the proliferative niches can facilitate the discovery and study of new targets specifically expressed by this proliferative and resistant subset of primary CLL cells. In order to further characterize the proliferative subset of primary CLL cells we analyzed the characteristics of proliferating subclones of CLL cells found in PB and compared it to the phenotype of primary CLL cells co-cultured ex vivo in conditions mimicking the microenvironment of the proliferative niches. Firstly, we compared actively proliferating CLL cells from 40 patients diagnosed with CLL with their quiescent counterpart. For this, we analyzed by flow cytometry (FC) the differential expression of ZAP-70, CD38, and the chemokine receptors CXCR4, CXCR5 and CCR7 in Ki-67 positive vs. negative CLL cells. Ki-67 positive CLL cells had higher expression levels of ZAP-70 and CD38, while the expression levels of all the chemokine receptors analyzed were significantly lower (Figure 1). This phenotype indicates that these cells may have an increased capability to respond to different survival and migration signals provided by the cellular microenvironment, since high expression of ZAP-70 and CD38 has been related to increased response to this kind of signaling. Moreover, downregulation of chemokine receptors would indicate recent stimulation by chemoatracting cytokines. Furthermore, with the aim of ex vivo mimic the microenvironment found in the proliferative centers, we co-cultured primary CLL cells from 27 patients with the BM-derived stromal cell line UE6E7T-2, 1μg/mL soluble CD40L and 1.5μg/mL CpG ODN, and analyzed the effects in terms of proliferation, modulation of surface molecules and development of chemoresistance. Of note, already 24 hours after co-culture, CLL cells increased their size, resembling stimulated B cells, and tended to be located in sparse clusters that included dividing cells, as observed after immunocytochemistry studies. In this setting, proliferative responses assessed by Ki-67 expression analyzed by FC were already observed after 24 hours (mean % Ki-67 positive cells: 1.48±0.28 in co-culture vs. 0.56±0.11 in suspension, P<0.01) and further increased after 48 hours (mean % Ki-67 positive cells: 2.89±0.51 in co-culture vs. 0.25±0.10 in suspension, P<0.01) and 72 hours (mean % Ki-67 positive cells: 7.68±2.11 in co-culture vs. 0.81±0.24 in suspension, P<0.001). Next, we analyzed the expression levels of ZAP-70, CD38 and the chemokine receptors CXCR4, CXCR5 and CCR7. Interestingly, the modulation of the expression of these molecules in co-cultured CLL cells correlated with the previously mentioned differential expression observed between the Ki-67 positive vs. negative compartments, indicating that the ex vivo co-culture highly resembles the conditions found in proliferative niches (Figure 1). Finally, in order to assess the role of microenvironment in chemoresistance, CLL cells were cultured in suspension and in co-culture for 48 hours and subsequently treated with increasing doses of fludarabine for 24 hours. Interestingly, the co-culture of CLL cells inhibited at such extend the capacity of fludarabine to induce apoptosis that it was not possible to calculate its lethal dose 50, whereas lethal dose 50 for fludarabine in CLL cells in suspension was 416μM (95%CI 125.5-1379). In conclusion, these results suggest that culturing CLL cells in the previously described conditions promotes proliferation and the acquisition of a phenotype which bears many similarities to the one found in actively proliferating CLL cells circulating in PB. This study provides a model for a co-culture system which might serve as a basis for the development and testing of new drugs that target the proliferative and drug resistant CLL cell compartment. Disclosures: No relevant conflicts of interest to declare.

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