Multivariate analyses of Ki‐67, cytokeratin 13 and cytokeratin 17 in diagnosis and prognosis of oral precancerous lesions

The objective: the finding of protein expression of apoptosis regulator BCL-2, Smooth Muscule Actin and the antigen Ki-67 in cervical intraepithelial neoplasia of different severity to optimize the diagnosis and prognosis of the disease. Patients and methods. The study involved 42 women of reproductive age with cervical intraepithelial the neoplasia of the cervix varying degrees applied to the doctor of cervical pathology Zhitomir regional oncologic dispensary. All women (n=42) were divided into groups. The first group included 15 patients (35.7%) with cervical intraepithelial neoplasia with mild. The second group included 13 women (31%) with cervical intraepithelial neoplasia a moderate degree. The third group was represented by patients with cervical intraepithelial neoplasia with severe – 14 respondents (33.3 per cent). Results. Marker BCL-2 in patients of the first group was positive in 7 patients (46.7%), Smooth Muscule Actin was positive in 9 patients (60%) and Ki-67 was diagnosed in 8 of the surveyed women (53.3%). In the second group of BCL-2 was positive in 8 patients (61.5%), Clone 124, Smooth Muscule Actin, Clone 1A4 was positive in 9 patients (69.2%), and Ki-67 was diagnosed in 12 of the surveyed women (92.3%). Marker BCL-2 in patients of the third group was positive in 12 patients (85.7%), Smooth Muscule Actin was positive in 10 patients (71.4%) and Ki-67 was diagnosed in 13 of the surveyed women (92.9% ). Conclusion. Carcinogenesis is associated with molecular genetic damage to the cervix. Some of the products of this process can be used as prognostic and diagnostic markers of tumor progression. Determination of protein expression of apoptosis regulator BCL-2, Smooth Muscule Actin and the antigen Ki-67 in cervical intraepithelial neoplasia makes it possible to accurately verify the diagnosis and to predict the course of pathological changes in the flat epithelium of the cervix. Key words: cervical intraepithelial neoplasia, cervical cancer, morphological diagnostics of precancerous lesions, BCL-2, Smooth Muscule Actin, Ki-67.

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Cytokeratin 13, Cytokeratin 17, Ki-67 and p53 Expression in Upper Layers of Epithelial Dysplasia Surrounding Tongue Squamous Cell Carcinoma

The Bulletin of Tokyo Dental College ◽

10.2209/tdcpublication.56.223 ◽

2015 ◽

Vol 56 (4) ◽

pp. 223-231 ◽

Cited By ~ 2

Author(s):

Akiko Matsuhira ◽

Sunaki Noguchi ◽

Kazumichi Sato ◽

Yoichi Tanaka ◽

Gou Yamamoto ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Tongue Squamous Cell Carcinoma ◽

Epithelial Dysplasia ◽

Ki 67 ◽

P53 Expression ◽

Cytokeratin 17 ◽

Cytokeratin 13

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Cytokeratin 13, Cytokeratin 17, and Ki-67 Expression in Human Acquired Cholesteatoma and Their Correlation With Its Destructive Capacity

Clinical and Experimental Otorhinolaryngology ◽

10.21053/ceo.2016.01263 ◽

2017 ◽

Vol 10 (3) ◽

pp. 213-220 ◽

Cited By ~ 2

Author(s):

Mahmood A. Hamed ◽

Seiichi Nakata ◽

Kazuya Shiogama ◽

Kenji Suzuki ◽

Ramadan H. Sayed ◽

...

Keyword(s):

Ki 67 ◽

Cytokeratin 17 ◽

Acquired Cholesteatoma ◽

Cytokeratin 13

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Interaction of tobacco chewing and smoking habit with interleukin 6 promoter polymorphism in oral precancerous lesions and oral cancer

European Archives of Oto-Rhino-Laryngology ◽

10.1007/s00405-021-06620-z ◽

2021 ◽

Author(s):

Yadvendra Shahi ◽

Sayali Mukherjee ◽

Fahad M. Samadi

Keyword(s):

Oral Cancer ◽

Interleukin 6 ◽

Precancerous Lesions ◽

Smoking Habit ◽

Promoter Polymorphism ◽

Oral Precancerous Lesions ◽

Tobacco Chewing

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The biogenesis and biological function of PIWI-interacting RNA in cancer

Journal of Hematology & Oncology ◽

10.1186/s13045-021-01104-3 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Silu Chen ◽

Shuai Ben ◽

Junyi Xin ◽

Shuwei Li ◽

Rui Zheng ◽

...

Keyword(s):

Cancer Diagnosis ◽

Precancerous Lesions ◽

Biological Activities ◽

Cancer Hallmarks ◽

Diagnosis And Prognosis ◽

Small Ncrnas ◽

Bioinformatics Databases ◽

Non Coding Rnas ◽

Underlying Mechanisms ◽

Proliferative Signaling

AbstractSmall non-coding RNAs (ncRNAs) are vital regulators of biological activities, and aberrant levels of small ncRNAs are commonly found in precancerous lesions and cancer. PIWI-interacting RNAs (piRNAs) are a novel type of small ncRNA initially discovered in germ cells that have a specific length (24–31 nucleotides), bind to PIWI proteins, and show 2′-O-methyl modification at the 3′-end. Numerous studies have revealed that piRNAs can play important roles in tumorigenesis via multiple biological regulatory mechanisms, including silencing transcriptional and posttranscriptional gene processes and accelerating multiprotein interactions. piRNAs are emerging players in the malignant transformation of normal cells and participate in the regulation of cancer hallmarks. Most of the specific cancer hallmarks regulated by piRNAs are involved in sustaining proliferative signaling, resistance to cell death or apoptosis, and activation of invasion and metastasis. Additionally, piRNAs have been used as biomarkers for cancer diagnosis and prognosis and have great potential for clinical utility. However, research on the underlying mechanisms of piRNAs in cancer is limited. Here, we systematically reviewed recent advances in the biogenesis and biological functions of piRNAs and relevant bioinformatics databases with the aim of providing insights into cancer diagnosis and clinical applications. We also focused on some cancer hallmarks rarely reported to be related to piRNAs, which can promote in-depth research of piRNAs in molecular biology and facilitate their clinical translation into cancer treatment.

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Histopathological specialized staining of oral lichen planus-induced fibrotic changes and surgical treatment of associated restricted mouth opening: a case report

Bulletin of the National Research Centre ◽

10.1186/s42269-021-00514-0 ◽

2021 ◽

Vol 45 (1) ◽

Author(s):

Atsushi Shudo

Keyword(s):

Surgical Treatment ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Histopathological Examination ◽

Mouth Opening ◽

Ki 67 ◽

Collagen Formation ◽

Immune Mediated ◽

Cytokeratin 13 ◽

Oral Lichen

Abstract Background Oral lichen planus is a chronic inflammatory and immune-mediated disease that affects the oral mucosa. Recent findings have suggested that oral lichen planus is often associated with submucosal fibrotic changes. Fibrotic changes in the buccal submucosa may cause restricted mouth opening. This report discusses the histopathological examination (including specialized staining) and surgical treatment for oral lichen planus-induced fibrotic changes. Case presentation Here, we describe a 63-year-old woman who had oral lichen planus with fibrotic changes. Her maximum mouth opening distance was approximately 30 mm due to submucosal fibrotic changes, and she exhibited gradual fibrosis progression. Histological examinations were performed to assess the oral lichen planus-induced fibrotic changes. Then, double Z-plasty were performed as treatment for restricted mouth opening. The immunohistochemical staining results were negative for cytokeratin 13 and positive in some layers for cytokeratin 17 and Ki-67/MIB-1. Masson's trichrome staining showed enhanced collagen formation. Postoperative mouth opening training enabled the patient to achieve a mouth opening distance of > 50 mm. Conclusion Our findings suggest that histopathological examination with specialized staining can aid in the evaluation of oral lichen planus-induced fibrotic changes, and that Z-plasty is effective for the treatment of restricted mouth opening due to oral lichen planus.

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The Role of Immunohistochemical Markers for the Diagnosis and Prognosis of Adrenocortical Neoplasms

Journal of Personalized Medicine ◽

10.3390/jpm11030208 ◽

2021 ◽

Vol 11 (3) ◽

pp. 208

Author(s):

Anna Angelousi ◽

Georgios Kyriakopoulos ◽

Fani Athanasouli ◽

Anastasia Dimitriadi ◽

Eva Kassi ◽

...

Keyword(s):

Transcriptome Analysis ◽

Cox Regression ◽

P53 Protein ◽

Immunohistochemical Analysis ◽

Ki 67 ◽

Diagnosis And Prognosis ◽

Immunohistochemical Markers ◽

Small Series ◽

Morphological Criteria ◽

Survival And Development

Adrenal cortical carcinoma (ACC) is a rare cancer with poor prognosis that needs to be distinguished from adrenocortical adenomas (ACAs). Although, the recently developed transcriptome analysis seems to be a reliable tool for the differential diagnosis of adrenocortical neoplasms, it is not widely available in clinical practice. We aim to evaluate histological and immunohistochemical markers for the distinction of ACCs from ACAs along with assessing their prognostic role. Clinical data were retrospectively analyzed from 37 patients; 24 archived, formalin-fixed, and paraffin-embedded ACC samples underwent histochemical analysis of reticulin and immunohistochemical analysis of p27, p53, Ki-67 markers and were compared with 13 ACA samples. Weiss and Helsinki scores were also considered. Kaplan−Meier and univariate Cox regression methods were implemented to identify prognostic effects. Altered reticulin pattern, Ki-67% labelling index and overexpression of p53 protein were found to be useful histopathological markers for distinguishing ACAs from ACCs. Among the studied markers, only pathological p53 nuclear protein expression was found to reach statistically significant association with poor survival and development of metastases, although in a small series of patients. In conclusion, altered reticulin pattern and p53/Ki-67 expression are useful markers for distinguishing ACCs from ACAs. Immunohistopathology alone cannot discriminate ACCs with different prognosis and it should be combined with morphological criteria and transcriptome analysis.

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