Transformasi Genetik Kedelai dengan Gen Proteinase Inhibitor II Menggunakan Teknik Penembakan Partikel

An experiment was conducted at the Molecular Biology and Genetic Engineering Laboratory of BB-Biogen, Bogor with an objective to obtain transgenic soybean plants containing the proteinase inhibitor II (pinII) gene. The experiment consisted of three steps, i.e., optimalization of the soybean transformation technique using the gus gene; transformation of soybean using the pinII gene, and molecular analysis of the transformed soybean plants. Two type of explants (young embryo and cotyledon) were bombarded with pRQ6 plasmid containing the gus gene with the following treatment: Helium gas pressure (1100 psi and 1300 psi), shoot distance (5 and 7 cm), and number of bombardment (1x and 2x). The result of gus assay indicated that the best bombardment was done on young cotyledon explants with 1100 psi Helium pressure, shoot distance 5 cm, and 1x bombardment. Transformation of the soybean explant using the pinII gene (inside the pTWa plasmid) was conducted using the best bombardment treatment from the first activity. Two plants from c.v. Wilis (WP1, WP2) and three plants from c.v. Tidar (TP1, TP2, TP3) were recovered from regeneration and selection of the transformed explants. Molecular analysis of the regenerated plants using the PCR technique showed that only WP2 contained the pinII gene. This plant was fertile and will be used for further evaluation.

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Signal interactions in pathogen and insect attack: expression of lipoxygenase, proteinase inhibitor II, and pathogenesis-related protein P4 in the tomato,Lycopersicon esculentum

Physiological and Molecular Plant Pathology ◽

10.1006/pmpp.1998.0192 ◽

1999 ◽

Vol 54 (3-4) ◽

pp. 97-114 ◽

Cited By ~ 143

Author(s):

A.L. Fidantsef ◽

M.J. Stout ◽

J.S. Thaler ◽

S.S. Duffey ◽

R.M. Bostock

Keyword(s):

Lycopersicon Esculentum ◽

Proteinase Inhibitor ◽

Related Protein ◽

Pathogenesis Related Protein ◽

Pathogenesis Related ◽

Proteinase Inhibitor Ii ◽

Insect Attack

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Characterization and Expression of a Wound-Inducible Proteinase Inhibitor II Gene from Potato

Tailoring Genes for Crop Improvement ◽

10.1007/978-1-4684-5329-4_11 ◽

1987 ◽

pp. 121-130

Author(s):

Robert W. Thornburg ◽

Gyn An ◽

Thomas A. Cleveland ◽

Clarence A. Ryan

Keyword(s):

Proteinase Inhibitor ◽

Proteinase Inhibitor Ii

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Selection of Trichoderma spp. strains for the control of Sclerotinia sclerotiorum in soybean

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2017001200002 ◽

2017 ◽

Vol 52 (12) ◽

pp. 1140-1148 ◽

Cited By ~ 7

Author(s):

Patrícia Elias Haddad ◽

Luis Garrigós Leite ◽

Cleusa Maria Mantovanello Lucon ◽

Ricardo Harakava

Keyword(s):

Sclerotinia Sclerotiorum ◽

Culture Medium ◽

Harmful Effect ◽

Soybean Seeds ◽

Trichoderma Spp ◽

Negative Effect ◽

Soybean Plants ◽

Selection Of

Abstract: The objective of this work was to evaluate, in vitro and in vivo, the potential of Trichoderma spp. strains to control Sclerotinia sclerotiorum in soybeans (Glycine max) and to perform the molecular identification of the best perfoming strains. The effect of 120 strains of Trichoderma spp. on the viability of S. sclerotiorum sclerotia was evaluated in vitro through immersion in suspension of conidia from the antagonists and plating in culture medium. The best performing strains were evaluated in vivo, in a greenhouse, for control of the pathogen inoculated on 'Pintado' soybean seeds and plants. Of the 120 strains tested in vitro, 22 strains of Trichoderma spp. caused 100% inhibition of sclerotia germination. In the greenhouse, five strains inhibited the negative effect of the pathogen on seed germination and two strains increased in up to 67% plant dry matter. The best performing strains were identified as T. koningiopsis (3 strains), T. asperelloides (3), T. atroviride (2), and T. virens (1). Trichoderma strains are able to protect soybean plants from the harmful effect of S. sclerotiorum and, at the same time, they can promote the growth of the aerial part in greenhouse conditions.

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The effect of mineral fertilizers and methods of basic soil tillage on the productivity of new promising soybean varieties

New Technologies ◽

10.47370/2072-0920-2020-16-5-87-94 ◽

2020 ◽

Vol 16 (5) ◽

pp. 87-94

Author(s):

K. H. Khatkov ◽

N. I. Mamsirov

Keyword(s):

Mineral Nutrition ◽

Cost Effective ◽

Optimal Dose ◽

Soil Tillage ◽

Mineral Fertilizers ◽

Organic Residues ◽

Soybean Plants ◽

Leguminous Crops ◽

Different Doses ◽

Selection Of

The agrotechnical significance of leguminous crops, in particular soybeans, consists in its ability to provide a huge bulk of vegetable protein and less nitrogen depletion of soils than non-legume crops [2]. Despite the fact that nitrogen symbiotically fixed by soybean plants is alienated with the harvest and taken out of the field with organic residues of leguminous crops, more nitrogen remains in the soil after them than with residues of other crops. In this regard soybeans are good predecessors in various stages of crop rotation. The article discusses topical issues of increasing productivity of leguminous crops, in particular, soybeans, when using different doses of ammophos against the background of its placement in various ways of main tillage of drained leached chernozems. In 2018–2019 studies were carried out to establish the influence of the optimal dose of mineral nutrition and the best method of soil cultivation on the productivity and quality indicators of soybeans of the French selection of the Amphora, Mentor and Isidor varieties. According to the results of the research, different effectiveness of the considered variants of the experiment was established. Thus, higher rates of soybean yield were noted in the later-maturing Isidor variety within 2,03–2,30 t/ha for plowing, and 1,70–1,91 for soil disking. The most cost-effective dose of mineral nutrition for all studied soybean varieties was the dose of 50 kg/ha of Ammophos (N6H26) against the background of plowing, where the profit from the increase in yield relative to the control was in the range of 2900–3650 rubles/ha, and against the background of disking soil the dose of Ammophos was 100 kg/ha (N12H52), where the profit was in the range of 1300–2050 rubles/ha.

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