scholarly journals Eliminasi Carnation Mottle Virus Menggunakan Senyawa Antiviral pada Kultur Jaringan Anyelir (Dianthus caryophyllus L.)

2016 ◽  
Vol 25 (3) ◽  
pp. 229
Author(s):  
Erniawati Diningsih ◽  
Gede Suastika ◽  
Tri Asmira Damayanti ◽  
S Susanto
Keyword(s):  
Dianthus Caryophyllus ◽  
Mottle Virus ◽  
Carnation Mottle Virus

Carnation mottle virus (CarMV) merupakan salah satu virus penting pada tanaman anyelir dan semua kultivar anyelir yang ditanam di Jawa Barat terinfeksi oleh virus ini.  Penelitian bertujuan mendapatkan metode eliminasi CarMV yang efektif untuk membebaskan planlet anyelir dari virus. Inisiasi eksplant terinfeksi dilakukan pada media MSO dan perbanyakan planlet dilakukan pada media perbanyakan MS yang mengandung 1,0 ppm BA dan 0,5 ppm kinetin (MSZ). Metode eliminasi CarMV yang diuji terdiri atas perlakuan 2-thiourasil dan amantadin dengan konsentrasi masing-masing 0, 5, 10, 15, 20, 25, dan 30 ppm, dan ribavirin 5 ppm sebagai pembanding.  Tunas apikal planlet ditanam pada media perlakuan (MSZA).  Setelah tunas tumbuh, meristem terminal diambil 0,5 mm untuk ditanam pada media MSZ.  Kultur meristem terminal dari planlet pada perlakuan 2- thiourasil menghasilkan planlet bebas virus sebesar 0 – 57%.  Konsentrasi 2-thiourasil 25 ppm menghasilkan persentase planlet bebas virus tertinggi, namun perlakuan tersebut toksik pada tanaman.  Perlakuan amantadin menghasilkan 25,0 – 54,55% planlet bebas virus. Di antara perlakuan yang diuji, perlakuan antiviral amantadin dengan konsentrasi 5 – 30 ppm lebih optimal menghasilkan planlet anyelir bebas CarMV dan tidak toksik terhadap tanaman. Perlakuan amantadin 5 – 20 ppm mampu menghambat virus lebih tinggi dibandingkan perlakuan 2-thiourasil pada konsentrasi yang sama. Amantadin 5 – 30 ppm menghasilkan tingkat penghambatan virus sebesar 42,94 – 59,57%, sedangkan 2-thiourasil sebesar -8,18 – 63,03%. Senyawa 2-thiourasil dan amantadin berpotensi sebagai agen antiviral untuk mendapatkan tanaman anyelir bebas CarMV.

scholarly journals Deteksi Cepat Carnation mottle virus pada Tanaman Anyelir (Dianthus caryophyllus L.)

2017 ◽  
Vol 27 (1) ◽  
pp. 95
Author(s):  
Erniawati Diningsih ◽  
Gede Suastika ◽  
Tri Asmira Damayanti ◽  
Slamet Susanto
Keyword(s):  
Dianthus Caryophyllus ◽  
Mottle Virus ◽  
Rt Pcr ◽  
One Step ◽  
Carnation Mottle Virus

<em>Carnation mottle virus </em>(CarMV) merupakan virus penting pada tanaman anyelir di Indonesia maupun di dunia.  Deteksi virus yang mudah dan cepat, diperlukan untuk memantau sumber induk anyelir bebas virus. Tujuan penelitian adalah mengevaluasi tiga metode preparasi RNA total yang mudah dan cepat dari tanaman anyelir sebagai templat <em>one step </em>RT-PCR.  Sumber RNA total adalah dari daun dan batang anyelir terinfeksi CarMV. Metode yang dievaluasi yaitu SDT, SEM, dan kit komersial sebagai pembanding. Optimasi dilakukan terhadap konsentrasi akhir primer (0.4 – 1.0 µM) dan MgCl<sub>2 </sub>(1.5 dan 2.0 mM).  Metode SDT dan SEM berhasil mendapat RNA total dari tanaman anyelir baik dari sampel daun maupun batang.  Keberhasilan yang didapat dengan metode SDT dan SEM sebanding dengan kit komersial.  <em>One step</em> RT-PCR RNA total yang digabungkan dengan metode SDT dan SEM menghasilkan intensitas DNA yang sebanding dengan kit komersial.  RNA total dari daun sebagai sumber templat <em>one step</em> RT-PCR terbaik dibandingkan batang. Preparasi RNA total dengan metode SDT dan SEM adalah metode cepat, mudah, dan murah dalam menyediakan templat <em>one step</em> RT-PCR. Konsentrasi primer 0.4 µM dan MgCl<sub>2 </sub>2 mM merupakan konsentrasi optimum dan menghasilkan hasil amplifikasi terbaik

Carnation mottle virus. [Distribution map].

2007 ◽  
Author(s):  
Keyword(s):  
Central America ◽  
Dianthus Caryophyllus ◽  
Uttar Pradesh ◽  
Mottle Virus ◽  
Distribution Map ◽  
Calla Lily ◽  
Virus Distribution ◽  
New Distribution ◽  
Distribution In Europe ◽  
Carnation Mottle Virus

Abstract A new distribution map is provided for Carnation mottle virus. Tombusviridae: Carmovirus. Hosts: carnation (Dianthus caryophyllus), sweet william (D. barbatus), Begonia elatior [B. hiemalis], B. cheinamantha, Daphne odorata, calla lily (Zantedeschia sp.) and garden lettuce (Lactuca sativa). Information is given on the geographical distribution in Europe (Belgium, Bulgaria, Czech Republic, Denmark, Finland, France, Germany, Hungary, Italy, Latvia, Lithuania, Moldova, Netherlands, Poland, Romania, Russia, Serbia, Spain, UK and Ukraine) and Asia (Fujian, Hebei, Jiangsu, Yunnan and Zhejiang, China; Gujarat and Uttar Pradesh, India; Iran, Israel; Honshu, Japan; Korea Republic; Lebanon; and Taiwan), Africa (Egypt), North America (British Columbia and Ontario, Canada; Mexico; and California, Colorado, Ohio, Pennsylvania and Wisconsin, USA), Central America and Carribean (Cuba), South America (Argentina, Chile, Colombia, Venezuela, and São Paulo, Brazil) and Oceania (Victoria, Australia, and New Zealand).

scholarly journals First Report of Carnation mottle virus in Calla lily (Zantedeschia spp.)

Plant Disease ◽  
2003 ◽  
Vol 87 (12) ◽  
pp. 1539-1539 ◽  
Author(s):  
C.-C. Chen ◽  
W.-F. Ko ◽  
C.-Y. Lin ◽  
F.-J. Jan ◽  
H. T. Hsu
Keyword(s):  
Mosaic Virus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Dianthus Caryophyllus ◽  
Spherical Particles ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infected Leaf ◽  
First Report ◽  
Cp Gene ◽  
Carnation Mottle Virus

Calla lilies are ornamental plants of major economic importance in Taiwan. They are grown in the central and northern areas of the island, and ≈3 million stems are shipped annually. Calla lilies are susceptible to several viruses (1). Infections by Cucumber mosaic virus, Dasheen mosaic virus, Turnip mosaic virus, and Watermelon silver mottle virus were reported in Taiwan. Recently, virus-like symptoms including yellow mottling, light yellow spot, yellow ringspot, and mosaic were observed on leaves of field-grown calla lilies from Changhua County, located in central Taiwan. In March 2001, a virus culture was isolated from diseased calla lilies and established in Chenopodium quinoa Willd. and Nicotiana benthamiana Domin. When inoculated with the virus, healthy calla lilies developed chlorotic spots that enlarged and fused to form large, yellow patches on inoculated leaves. Symptoms were similar to those on the naturally infected plants observed in the fields. The virus induced chlorotic local lesions on C. quinoa, C. ficifolium Sm., C. amaranticolor Coste & Reyn, Cucurbita moschata Duchesne ex Poir, Lisianthus russellianum (Don.) Griseb, Phaseolus angularis Wight, Vigna angularis Willd., and V. radiata (L.) Wilczek. In addition to the localized chlorotic spots on inoculated leaves, systemic invasion of the virus was also observed 8 to 10 days postinoculation in Dianthus caryophyllus L., D. chinensis L., and Glycine max Merr. In N. benthamiana, the only symptom observed was systemic wilting. Examination of 2% of uranyl-acetate-stained samples using electron microscopy revealed the presence of spherical particles ≈34 to 35 nm in diameter in crude extracts of leaves of diseased calla lilies, or infected C. quinoa. Similar particles were also observed in the cytoplasm but not in the nuclei in ultrathin sections of virus-infected leaf tissues of C. quinoa and N. benthamiana. Differential centrifugation followed by sucrose density gradient centrifugation of tissue extracts of infected C. quinoa yielded virions with similar size. Sodium dodesyl sulfate polyacrylamide gel electrophoresis of the purified virus showed a single structural polypeptide ith a Mr of 41.6 kDa. The viral antigen reacted positively with its homologous antiserum and an antiserum against Carnation mottle virus (CarMV; Agdia, Inc., Elkhart, IN) in double antibody sandwich enzyme-linked immunosorbent assay. Using primers 5′-CTCCATGGTCATGGAA(A/G)ATAAA GGAGAA and 3′-CAACAAATATCCTACACTGTCCTAGGTG specific to the coat protein (CP) gene of CarMV, an expected viral CP gene product of 1.05 kb was amplified by reverse transcription-polymerase chain reaction from total RNA isolated from infected N. benthamiana. Comparisons of the 1,047-nucleotide CP gene with those of 15 CarMV isolates available in GenBank showed 94.6 to 98.2% nucleotide identity and 94.8 to 96.8% amino acid identity. Results from current studies indicate that the virus infecting calla lilies is an isolate of CarMV. To our knowledge, this is the first report of CarMV infection in calla lilies. The occurrence of CarMV in calla lilies has direct implication for the economically important nursery and floral industry in Taiwan. Reference: (1) F. W. Zettler and R. D. Hartman. Dieffenbachia, Caladium, and Zantedeschia. Pages 464–470 in: Virus and Virus-Like Diseases of Bulb and Flower Crops. G. Loebenstein, R. H. Lawson, and A. A. Brunt, eds. John Wiley and Sons, West Sussex, U.K., 1995.

CLONING OF COMPLETE CARNATION MOTTLE VIRUS cDNA AND ITS INFECTIVITY

2002 ◽  
pp. 149-154 ◽  
Author(s):  
A-P. Zhang ◽  
H-Q. Zhu ◽  
S-Q. Yu ◽  
L. Yang ◽  
Y. Lou
Keyword(s):  
Mottle Virus ◽  
Virus Cdna ◽  
Carnation Mottle Virus
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